(Circular) (Six-base) MAPSORT of: pFN21AB5676.seq Check: 5277 from: 1 to: 7123 pFN21AB5676 7123 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4933 278 Size: 53 83 186 936 3397 2468 Fragments arranged by size: 3397 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2403 4389 4466 1937 Size: 466 1986 77 4594 Fragments arranged by size: 4594 1986 466 77 Acc65I G'GTAC_C Cuts at: 4444 4444 Size: 7123 AclI AA'CG_TT Cuts at: 1563 5250 5623 1563 Size: 3687 373 3063 Fragments arranged by size: 3687 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3131 3536 4274 4300 4311 5186 6570 6838 Size: 1499 405 738 26 11 875 1384 268 Cuts at: 6838 1632 Size: 1917 Fragments arranged by size: 1917 1499 1384 875 738 405 268 26 11 AflII C'TTAA_G Cuts at: 820 1017 2408 820 Size: 197 1391 5535 Fragments arranged by size: 5535 1391 197 AflIII A'CryG_T Cuts at: 1203 3022 6022 6999 1203 Size: 1819 3000 977 1327 Fragments arranged by size: 3000 1819 1327 977 AgeI A'CCGG_T Cuts at: 5954 5954 Size: 7123 AhdI GACnn_n'nnGTC Cuts at: 5852 5852 Size: 7123 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7123 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3243 3262 1184 Size: 2059 19 5045 Fragments arranged by size: 5045 2059 19 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4007 4039 4007 Size: 32 7091 AlwNI CAG_nnn'CTG Cuts at: 3257 3478 3817 4190 4866 6438 3257 Size: 221 339 373 676 1572 3942 Fragments arranged by size: 3942 1572 676 373 339 221 ApaLI G'TGCA_C Cuts at: 5180 6336 5180 Size: 1156 5967 ApoI r'AATT_y Cuts at: 1493 3114 3509 4432 4658 4810 6919 1493 Size: 1621 395 923 226 152 2109 1697 Fragments arranged by size: 2109 1697 1621 923 395 226 152 AseI AT'TA_AT Cuts at: 160 1032 5677 160 Size: 872 4645 1606 Fragments arranged by size: 4645 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7123 AvaI C'yCGr_G Cuts at: 1870 1943 2769 4448 1870 Size: 73 826 1679 4545 Fragments arranged by size: 4545 1679 826 73 AvrII C'CTAG_G Cuts at: 2290 2290 Size: 7123 BamHI G'GATC_C Cuts at: 4453 4820 4453 Size: 367 6756 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3415 4444 5899 618 Size: 325 217 627 1628 1029 1455 1842 Fragments arranged by size: 1842 1628 1455 1029 627 325 217 BanII G_rGCy'C Cuts at: 1391 4442 1391 Size: 3051 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3441 4404 928 Size: 971 1542 963 3647 Fragments arranged by size: 3647 1542 971 963 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5314 5348 1903 Size: 34 3377 34 3678 Fragments arranged by size: 3678 3377 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2354 3498 5015 6225 2354 Size: 1144 1517 1210 3252 Fragments arranged by size: 3252 1517 1210 1144 BclI T'GATC_A Cuts at: 1548 2808 3922 1548 Size: 1260 1114 4749 Fragments arranged by size: 4749 1260 1114 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5734 136 Size: 107 122 71 631 605 4062 1525 Fragments arranged by size: 4062 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7118 7118 Size: 7123 BlpI GC'TnA_GC Cuts at: 4540 4540 Size: 7123 Bme1580I G_kGCm'C Cuts at: 1790 3022 5184 6340 1790 Size: 1232 2162 1156 2573 Fragments arranged by size: 2573 2162 1232 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7123 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3992 4347 4854 Size: 416 53 83 399 24 2575 355 507 Cuts at: 4854 5812 442 Size: 958 1753 Fragments arranged by size: 2575 1753 958 507 416 399 355 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2645 1056 Size: 1589 5534 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2034 2520 2811 4074 5783 1239 Size: 795 486 291 1263 1709 2579 Fragments arranged by size: 2579 1709 1263 795 486 291 Bpu10I CC'TnA_GC Cuts at: 3639 3639 Size: 7123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3391 3959 5248 6113 6411 3391 Size: 568 1289 865 298 4103 Fragments arranged by size: 4103 1289 865 568 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3302 5786 882 Size: 617 1803 2484 2219 Fragments arranged by size: 2484 2219 1803 617 BsaAI yAC'GTr Cuts at: 493 1204 4914 493 Size: 711 3710 2702 Fragments arranged by size: 3710 2702 711 BsaBI GATnn'nnATC Cuts at: 7117 7117 Size: 7123 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4930 5312 5947 275 Size: 53 83 186 936 3397 382 635 1451 Fragments arranged by size: 3397 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2442 3360 5556 5954 6228 6375 6786 2442 Size: 918 2196 398 274 147 411 2779 Fragments arranged by size: 2779 2196 918 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3608 3638 1178 Size: 30 165 30 2205 30 4663 Fragments arranged by size: 4663 2205 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2891 1186 Size: 1705 5418 BseYI C'CCAG_C Cuts at: 2130 3067 6326 6822 2130 Size: 937 3259 496 2431 Fragments arranged by size: 3259 2431 937 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6550 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4573 5334 6362 1540 Size: 275 186 2572 761 1028 2301 Fragments arranged by size: 2572 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3251 3501 5184 5269 6340 3251 Size: 250 1683 85 1071 4034 Fragments arranged by size: 4034 1683 1071 250 85 BsmI GAATG_Cn' Cuts at: 2896 3611 4634 4727 2896 Size: 715 1023 93 5292 Fragments arranged by size: 5292 1023 715 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2188 2188 Size: 7123 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3022 3251 3501 4442 5184 5269 6340 Size: 399 1232 229 250 941 742 85 1071 Cuts at: 6340 1391 Size: 2174 Fragments arranged by size: 2174 1232 1071 941 742 399 250 229 85 BspEI T'CCGG_A Cuts at: 2442 6786 2442 Size: 4344 2779 BspHI T'CATG_A Cuts at: 2826 3327 5012 6742 2826 Size: 501 1685 1730 3207 Fragments arranged by size: 3207 1730 1685 501 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3993 4478 844 Size: 3149 485 3489 Fragments arranged by size: 3489 3149 485 BsrBI CCG'CTC Cuts at: 1394 1823 2968 5010 1394 Size: 429 1145 2042 3507 Fragments arranged by size: 3507 2042 1145 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3594 5618 5792 65 Size: 1183 2346 2024 174 1396 Fragments arranged by size: 2346 2024 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3876 5767 5954 1700 Size: 110 2066 1891 187 2869 Fragments arranged by size: 2869 2066 1891 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7123 BssHII G'CGCG_C Cuts at: 1925 4232 1925 Size: 2307 4816 BssSI C'ACGA_G Cuts at: 3759 5183 6195 3759 Size: 1424 1012 4687 Fragments arranged by size: 4687 1424 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7123 BstBI TT'CG_AA Cuts at: 5927 5927 Size: 7123 BstEII G'GTnAC_C Cuts at: 1184 3424 1184 Size: 2240 4883 BstXI CCAn_nnnn'nTGG Cuts at: 4012 4012 Size: 7123 BstYI r'GATC_y Cuts at: 1972 2509 3150 3286 3813 4194 4453 4820 5205 Size: 537 641 136 527 381 259 367 385 Cuts at: 5205 5222 6674 6760 6772 7118 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 641 537 527 385 381 367 346 259 136 86 17 12 BstZ17I GTA'TAC Cuts at: 2404 4390 2404 Size: 1986 5137 BtgI C'CryG_G Cuts at: 513 1065 2004 2467 3405 4161 513 Size: 552 939 463 938 756 3475 Fragments arranged by size: 3475 939 938 756 552 463 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2914 487 Size: 683 56 1688 4696 Fragments arranged by size: 4696 1688 683 56 BtsI GCAGTG_nn' Cuts at: 2551 3392 4369 4390 4639 5433 5453 2551 Size: 841 977 21 249 794 20 4221 Fragments arranged by size: 4221 977 841 794 249 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7123 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3742 3777 529 Size: 35 3178 35 3875 Fragments arranged by size: 3875 3178 35 35 DraI TTT'AAA Cuts at: 4427 4427 Size: 7123 DraIII CAC_nnn'GTG Cuts at: 3654 3654 Size: 7123 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3915 6130 809 Size: 507 2599 2215 1802 Fragments arranged by size: 2599 2215 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3585 3913 4095 Size: 54 1425 50 275 18 1755 328 182 Cuts at: 4095 4570 5459 7088 8 Size: 475 889 1629 43 Fragments arranged by size: 1755 1629 1425 889 475 328 275 182 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4570 1537 Size: 275 2758 4090 Fragments arranged by size: 4090 2758 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2186 2399 2583 4167 5053 1353 Size: 833 213 184 1584 886 3423 Fragments arranged by size: 3423 1584 886 833 213 184 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5710 6084 6230 387 Size: 1064 138 4121 374 146 1280 Fragments arranged by size: 4121 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2520 2811 3131 3536 4074 4274 Size: 393 402 486 291 320 405 538 200 Cuts at: 4274 4300 4311 5186 5783 6570 6838 1239 Size: 26 11 875 597 787 268 1524 Fragments arranged by size: 1524 875 787 597 538 486 405 402 393 320 291 268 200 26 11 EcoNI CCTnn'n_nnAGG Cuts at: 2693 2718 2748 3194 2693 Size: 25 30 446 6622 Fragments arranged by size: 6622 446 30 25 EcoO109I rG'GnC_Cy Cuts at: 3445 3635 3445 Size: 190 6933 EcoRI G'AATT_C Cuts at: 3114 4432 4810 3114 Size: 1318 378 5427 Fragments arranged by size: 5427 1318 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2434 2434 Size: 7123 FspI TGC'GCA Cuts at: 3989 5629 3989 Size: 1640 5483 HaeII r_GCGC'y Cuts at: 1678 3870 6270 1678 Size: 2192 2400 2531 Fragments arranged by size: 2531 2400 2192 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2024 2056 2576 2608 2800 Size: 32 135 32 452 32 520 32 192 Cuts at: 2800 2832 3821 3853 3941 3973 5764 5796 5838 Size: 32 989 32 88 32 1791 32 42 Cuts at: 5838 5870 1373 Size: 32 2626 Fragments arranged by size: 2626 1791 989 520 452 192 135 88 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2991 4467 4713 669 Size: 988 281 1053 1476 246 3079 Fragments arranged by size: 3079 1476 1053 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7123 HpaI GTT'AAC Cuts at: 4713 4713 Size: 7123 KpnI G_GTAC'C Cuts at: 4448 4448 Size: 7123 MfeI C'AATT_G Cuts at: 2700 4722 2700 Size: 2022 5101 MluI A'CGCG_T Cuts at: 6999 6999 Size: 7123 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6237 6421 1305 Size: 4932 184 2007 Fragments arranged by size: 4932 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3587 4097 10 Size: 54 1425 343 1755 510 3036 Fragments arranged by size: 3036 1755 1425 510 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3219 3225 5081 5440 5599 518 Size: 648 2053 6 1856 359 159 2042 Fragments arranged by size: 2053 2042 1856 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 4539 5216 6364 6609 1606 Size: 2933 677 1148 245 2120 Fragments arranged by size: 2933 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 4161 513 Size: 552 939 2157 3475 Fragments arranged by size: 3475 2157 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7123 NheI G'CTAG_C Cuts at: 1052 2641 1052 Size: 1589 5534 NotI GC'GGCC_GC Cuts at: 4570 4570 Size: 7123 NspI r_CATG'y Cuts at: 1132 1229 3026 3224 3838 4481 6026 1132 Size: 97 1797 198 614 643 1545 2229 Fragments arranged by size: 2229 1797 1545 643 614 198 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7123 PciI A'CATG_T Cuts at: 3022 6022 3022 Size: 3000 4123 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7123 PfoI T'CCnGG_A Cuts at: 2421 2743 2421 Size: 322 6801 PmeI GTTT'AAAC Cuts at: 4427 4427 Size: 7123 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5192 5224 6705 6737 5192 Size: 32 1481 32 5578 Fragments arranged by size: 5578 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3635 3635 Size: 7123 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7123 PsiI TTA'TAA Cuts at: 4693 4693 Size: 7123 PspXI vC'TCGA_Gb Cuts at: 2769 2769 Size: 7123 PstI C_TGCA'G Cuts at: 830 2633 4475 830 Size: 1803 1842 3478 Fragments arranged by size: 3478 1842 1803 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7123 SalI G'TCGA_C Cuts at: 1936 4465 1936 Size: 2529 4594 SbfI CC_TGCA'GG Cuts at: 4475 4475 Size: 7123 ScaI AGT'ACT Cuts at: 5371 7083 5371 Size: 1712 5411 SfcI C'TryA_G Cuts at: 826 1045 2629 2775 3126 3470 4471 5606 6287 Size: 219 1584 146 351 344 1001 1135 681 Cuts at: 6287 6478 826 Size: 191 1471 Fragments arranged by size: 1584 1471 1135 1001 681 351 344 219 191 146 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7123 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7123 SmaI CCC'GGG Cuts at: 1872 4450 1872 Size: 2578 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2408 2769 3370 3974 5227 6128 Size: 197 926 465 361 601 604 1253 901 Cuts at: 6128 6390 820 Size: 262 1553 Fragments arranged by size: 1553 1253 926 901 604 601 465 361 262 197 SnaBI TAC'GTA Cuts at: 493 4914 493 Size: 4421 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7123 SphI G_CATG'C Cuts at: 1132 4481 1132 Size: 3349 3774 SspI AAT'ATT Cuts at: 5 52 5047 5 Size: 47 4995 2081 Fragments arranged by size: 4995 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7123 StyI C'CwwG_G Cuts at: 513 1065 2004 2290 3537 3753 4005 4161 4562 Size: 552 939 286 1247 216 252 156 401 Cuts at: 4562 513 Size: 3074 Fragments arranged by size: 3074 1247 939 552 401 286 252 216 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3215 3516 3813 5150 5167 5320 5505 Size: 1255 1635 301 297 1337 17 153 185 Cuts at: 5505 5950 325 Size: 445 1498 Fragments arranged by size: 1635 1498 1337 1255 445 301 297 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5369 7081 96 Size: 274 80 33 51 1443 3392 1712 138 Fragments arranged by size: 3392 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4324 4356 7083 7115 4324 Size: 32 2727 32 4332 Fragments arranged by size: 4332 2727 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3800 1316 Size: 2484 4639 XbaI T'CTAG_A Cuts at: 4459 4459 Size: 7123 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3426 4343 3426 Size: 917 6206 XhoI C'TCGA_G Cuts at: 1943 2769 1943 Size: 826 6297 XmaI C'CCGG_G Cuts at: 1870 4448 1870 Size: 2578 4545 XmnI GAAnn'nnTTC Cuts at: 5252 5252 Size: 7123 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4931 276 Size: 53 83 186 936 3397 2468 Fragments arranged by size: 3397 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI