Restriction map for
pFN21AB5670
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccII AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BciVI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaI BsiEI BsiWI BsmBI BsoBI
BspDI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I FauI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
StuI Tsp45I Tth111I Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 2531 GTAGAC GT^[AC][GT]AC
AccIII 47 TCCGGA T^CCGGA
AciI 1635 GCGG CCGC(-3/-1)
AclI 323 AACGTT AA^CGTT
AflIII 2261 ACATGT A^C[GA][CT]GT
ApaI 144 GGGCCC GGGCC^C
AseI 394 ATTAAT AT^TAAT
AsnI 394 ATTAAT AT^TAAT
AvaII 60 GGACC G^G[AT]CC
BamHI 1806 GGATCC G^GATCC
BclI 1154 TGATCA T^GATCA
BcnI 2025 CCCGG CC^[GC]GG
BlpI 1426 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1426 GCTCAGC GC^T[ACGT]AGC
BsaWI 47 TCCGGA [AT]^CCGG[AT]
BseAI 47 TCCGGA T^CCGGA
BseRI 301 GAGGAG GAGGAG(10/8)
BsmAI 1879 GAGAC GTCTC(1/5)
BspEI 47 TCCGGA T^CCGGA
BspHI 2355 TCATGA T^CATGA
BspLU11I 2261 ACATGT A^CATGT
BstAPI 2429 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1209 CCAGGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1426 GCTCAGC GC^T[ACGT]AGC
DraII 143 AGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 210 CCGTGG C^C[GA][CT]GG
EcoO109I 143 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 672 GAATTC G^AATTC
EcoRV 364 GATATC GAT^ATC
FbaI 1154 TGATCA T^GATCA
MroI 47 TCCGGA T^CCGGA
NciI 2025 CCCGG CC^[GC]GG
PacI 391 TTAATTAA TTAAT^TAA
PshBI 394 ATTAAT AT^TAAT
Psp1406I 323 AACGTT AA^CGTT
RcaI 2355 TCATGA T^CATGA
SacI 187 GAGCTC GAGCT^C
SapI 2192 GAAGAGC GCTCTTC(1/4)
SspI 2074 AATATT AAT^ATT
SwaI 1410 ATTTAAAT ATTT^AAAT
XbaI 1170 TCTAGA T^CTAGA
XcmI 1907 CCAGAGCACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 2093 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2704 CAGACACTG
BanII 144 GGGCCC G[GA]GC[CT]^C
187 GAGCTC
BbsI 51 GAAGAC GAAGAC(2/6)
1834 GAAGAC
BcgI 2596 CGACAGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1469 GCAAAGACCTCG
BglII 594 AGATCT A^GATCT
1752 AGATCT
BpuAI 51 GAAGAC GAAGAC(2/6)
1834 GAAGAC
BsgI 112 GTGCAG GTGCAG(16/14)
452 GTGCAG
Bsp1407I 710 TGTACA T^GTACA
2264 TGTACA
BspMI 238 GCAGGT ACCTGC(4/8)
448 GCAGGT
BsrGI 710 TGTACA T^GTACA
2264 TGTACA
EcoNI 92 CCTGGTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1062 CCTTAAAAAGG
EcoT14I 1268 CCTTGG C^C[AT][AT]GG
2444 CCTTGG
EcoT22I 1557 ATGCAT ATGCA^T
2048 ATGCAT
HapII 48 CCGG C^CGG
2026 CCGG
HpaII 48 CCGG C^CGG
2026 CCGG
MspI 48 CCGG C^CGG
2026 CCGG
NsiI 1557 ATGCAT ATGCA^T
2048 ATGCAT
SmlI 172 CTCAAG C^T[CT][GA]AG
598 CTTGAG
SpeI 1129 ACTAGT A^CTAGT
2224 ACTAGT
SphI 1555 GCATGC GCATG^C
1668 GCATGC
SspBI 710 TGTACA T^GTACA
2264 TGTACA
StyI 1268 CCTTGG C^C[AT][AT]GG
2444 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AspHI BbvI BfaI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI HaeIII HgaI
HinfI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII XmnI