Restriction map for
pFN21AB5663
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BsrI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MflI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AfaI 48 GTAC GT^AC
AocI 648 CCTGAGG CC^T[ACGT]AGG
AvaI 400 CCCGAG C^[CT]CG[GA]G
BalI 239 TGGCCA TGG^CCA
BbsI 229 GTCTTC GAAGAC(2/6)
BclI 707 TGATCA T^GATCA
BpuAI 229 GTCTTC GAAGAC(2/6)
BsgI 142 GTGCAG GTGCAG(16/14)
BsmFI 872 GGGAC GGGAC(10/14)
BsoBI 400 CCCGAG C^[CT]CG[GA]G
BspMI 109 ACCTGC ACCTGC(4/8)
BsrDI 460 GCAATG GCAATG(2/0)
BssHII 113 GCGCGC G^CGCGC
Bsu36I 648 CCTGAGG CC^T[ACGT]AGG
DraII 152 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 602 CCATGG C^C[GA][CT]GG
EagI 359 CGGCCG C^GGCCG
EarI 823 GAAGAG CTCTTC(1/4)
EclXI 359 CGGCCG C^GGCCG
Eco52I 359 CGGCCG C^GGCCG
Eco81I 648 CCTGAGG CC^T[ACGT]AGG
EcoNI 774 CCTGCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 152 AGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 707 TGATCA T^GATCA
HinfI 766 GAGTC G^A[ACGT]TC
HphI 432 GGTGA GGTGA(8/7)
Ksp632I 823 GAAGAG CTCTTC(1/4)
MaeIII 716 GTGAC ^GT[ACGT]AC
MluNI 239 TGGCCA TGG^CCA
MscI 239 TGGCCA TGG^CCA
NcoI 602 CCATGG C^CATGG
NspI 317 GCATGT [GA]CATG^[CT]
PleI 766 GAGTC GAGTC(4/5)
PpuMI 152 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 295 CTGCAG CTGCA^G
PvuII 298 CAGCTG CAG^CTG
RsaI 48 GTAC GT^AC
SfcI 295 CTGCAG C^T[GA][CT]AG
SmlI 442 CTCAAG C^T[CT][GA]AG
StuI 639 AGGCCT AGG^CCT
TaqI 752 TCGA T^CGA
Tsp45I 716 GTGAC ^GT[GC]AC
TspRI 632 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 752 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwNI 10 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
298 CAGCTGCTG
BanII 274 GAGCCC G[GA]GC[CT]^C
654 GAGCCC
BcnI 75 CCGGG CC^[GC]GG
732 CCGGG
BmyI 274 GAGCCC G[AGT]GC[ACT]^C
654 GAGCCC
BsaI 106 GAGACC GGTCTC(1/5)
343 GAGACC
BsiEI 72 CGACCG CG[GA][CT]^CG
359 CGGCCG
BsmAI 106 GAGAC GTCTC(1/5)
343 GAGAC
Bsp1286I 274 GAGCCC G[AGT]GC[ACT]^C
654 GAGCCC
DdeI 332 CTAAG C^T[ACGT]AG
649 CTGAG
EcoT14I 602 CCATGG C^C[AT][AT]GG
803 CCAAGG
MaeII 866 ACGT A^CGT
875 ACGT
NciI 75 CCGGG CC^[GC]GG
732 CCGGG
StyI 602 CCATGG C^C[AT][AT]GG
803 CCAAGG
XcmI 182 CCACCTGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
242 CCATGAACTACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BpmI BsaJI BseRI BsiYI BslI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII EaeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TseI