(Circular) (Six-base) MAPSORT of: pFN21AB5663.seq Check: 3199 from: 1 to: 5590 pFN21AB5663 5590 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3400 278 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2933 1937 Size: 996 4594 Acc65I G'GTAC_C Cuts at: 2911 2911 Size: 5590 AclI AA'CG_TT Cuts at: 1563 3717 4090 1563 Size: 2154 373 3063 Fragments arranged by size: 3063 2154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2120 3653 5037 5305 1632 Size: 488 1533 1384 268 1917 Fragments arranged by size: 1917 1533 1384 488 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5393 AflIII A'CryG_T Cuts at: 1203 4489 5466 1203 Size: 3286 977 1327 Fragments arranged by size: 3286 1327 977 AgeI A'CCGG_T Cuts at: 4421 4421 Size: 5590 AhdI GACnn_n'nnGTC Cuts at: 4319 4319 Size: 5590 AleI CACnn'nnGTG Cuts at: 1166 2579 2870 1166 Size: 1413 291 3886 Fragments arranged by size: 3886 1413 291 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5590 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2001 2033 2001 Size: 32 5558 AlwNI CAG_nnn'CTG Cuts at: 2020 2308 3333 4905 2020 Size: 288 1025 1572 2705 Fragments arranged by size: 2705 1572 1025 288 ApaLI G'TGCA_C Cuts at: 3647 4803 3647 Size: 1156 4434 ApoI r'AATT_y Cuts at: 1493 2899 3125 3277 5386 1493 Size: 1406 226 152 2109 1697 Fragments arranged by size: 2109 1697 1406 226 152 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2405 2915 1870 Size: 73 462 510 4545 Fragments arranged by size: 4545 510 462 73 BamHI G'GATC_C Cuts at: 2920 3287 2920 Size: 367 5223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2911 4366 618 Size: 325 217 627 1124 1455 1842 Fragments arranged by size: 1842 1455 1124 627 325 217 BanII G_rGCy'C Cuts at: 1391 2283 2663 2909 1391 Size: 892 380 246 4072 Fragments arranged by size: 4072 892 380 246 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2227 928 Size: 971 328 4291 Fragments arranged by size: 4291 971 328 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3781 3815 1903 Size: 34 1844 34 3678 Fragments arranged by size: 3678 1844 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3482 4692 3482 Size: 1210 4380 BclI T'GATC_A Cuts at: 1548 2712 1548 Size: 1164 4426 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4201 136 Size: 107 122 71 631 605 2529 1525 Fragments arranged by size: 2529 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5585 5585 Size: 5590 BlpI GC'TnA_GC Cuts at: 3007 3007 Size: 5590 Bme1580I G_kGCm'C Cuts at: 1790 3651 4807 1790 Size: 1861 1156 2573 Fragments arranged by size: 2573 1861 1156 BmgBI CAC'GTC Cuts at: 1318 2872 2881 1318 Size: 1554 9 4027 Fragments arranged by size: 4027 1554 9 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3321 4279 442 Size: 416 53 83 399 24 1904 958 1753 Fragments arranged by size: 1904 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2297 2689 2789 4250 1239 Size: 980 78 392 100 1461 2579 Fragments arranged by size: 2579 1461 980 392 100 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2432 3715 4580 4878 2432 Size: 1283 865 298 3144 Fragments arranged by size: 3144 1283 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2105 2342 4253 882 Size: 617 606 237 1911 2219 Fragments arranged by size: 2219 1911 617 606 237 BsaAI yAC'GTr Cuts at: 493 1204 3381 493 Size: 711 2177 2702 Fragments arranged by size: 2702 2177 711 BsaBI GATnn'nnATC Cuts at: 5584 5584 Size: 5590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3397 3779 4414 275 Size: 53 83 186 936 1864 382 635 1451 Fragments arranged by size: 1864 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4023 4421 4695 4842 5253 4023 Size: 398 274 147 411 4360 Fragments arranged by size: 4360 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2001 2031 2483 2513 1178 Size: 30 165 30 598 30 452 30 4255 Fragments arranged by size: 4255 598 452 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2123 2240 2243 2423 2534 2671 2834 Size: 874 63 117 3 180 111 137 163 Cuts at: 2834 2837 2846 2849 2852 2855 1186 Size: 3 9 3 3 3 3921 Fragments arranged by size: 3921 874 180 163 137 117 111 63 9 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2308 2790 4793 5289 2308 Size: 482 2003 496 2609 Fragments arranged by size: 2609 2003 496 482 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2168 1163 Size: 573 432 4585 Fragments arranged by size: 4585 573 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2080 2367 3040 3801 4829 1540 Size: 275 186 79 287 673 761 1028 2301 Fragments arranged by size: 2301 1028 761 673 287 275 186 79 BsiHKAI G_wGCw'C Cuts at: 3651 3736 4807 3651 Size: 85 1071 4434 Fragments arranged by size: 4434 1071 85 BsmI GAATG_Cn' Cuts at: 3101 3194 3101 Size: 93 5497 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2663 2909 3651 3736 4807 1391 Size: 399 493 380 246 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 493 399 380 246 85 BspEI T'CCGG_A Cuts at: 5253 5253 Size: 5590 BspHI T'CATG_A Cuts at: 3479 5209 3479 Size: 1730 3860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2123 2945 844 Size: 1279 822 3489 Fragments arranged by size: 3489 1279 822 BsrBI CCG'CTC Cuts at: 1394 1823 3477 1394 Size: 429 1654 3507 Fragments arranged by size: 3507 1654 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2472 4085 4259 65 Size: 1183 1224 1613 174 1396 Fragments arranged by size: 1613 1396 1224 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4234 4421 1700 Size: 110 2424 187 2869 Fragments arranged by size: 2869 2424 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 2118 1925 Size: 193 5397 BssSI C'ACGA_G Cuts at: 3650 4662 3650 Size: 1012 4578 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5590 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5590 BstYI r'GATC_y Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585 Size: 948 367 385 17 1452 86 12 346 Cuts at: 5585 1972 Size: 1977 Fragments arranged by size: 1977 1452 948 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2654 2654 Size: 5590 BtgI C'CryG_G Cuts at: 513 1065 2004 2607 513 Size: 552 939 603 3496 Fragments arranged by size: 3496 939 603 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4851 Fragments arranged by size: 4851 683 56 BtsI GCAGTG_nn' Cuts at: 2643 3106 3900 3920 2643 Size: 463 794 20 4313 Fragments arranged by size: 4313 794 463 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5555 DraI TTT'AAA Cuts at: 2894 2894 Size: 5590 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4597 809 Size: 507 3281 1802 Fragments arranged by size: 3281 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2244 2364 2460 Size: 54 1425 50 275 18 414 120 96 Cuts at: 2460 2631 3037 3926 5555 8 Size: 171 406 889 1629 43 Fragments arranged by size: 1629 1425 889 414 406 275 171 120 96 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2364 3037 1537 Size: 275 552 673 4090 Fragments arranged by size: 4090 673 552 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2823 3520 1353 Size: 1470 697 3423 Fragments arranged by size: 3423 1470 697 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2033 2120 2531 2589 4177 4551 Size: 1064 138 444 87 411 58 1588 374 Cuts at: 4551 4697 387 Size: 146 1280 Fragments arranged by size: 1588 1280 1064 444 411 374 146 138 87 58 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2219 2297 2689 2789 3653 4250 Size: 393 488 99 78 392 100 864 597 Cuts at: 4250 5037 5305 1239 Size: 787 268 1524 Fragments arranged by size: 1524 864 787 597 488 393 392 268 100 99 78 EcoNI CCTnn'n_nnAGG Cuts at: 2783 2783 Size: 5590 EcoO109I rG'GnC_Cy Cuts at: 2158 2158 Size: 5590 EcoRI G'AATT_C Cuts at: 2899 3277 2899 Size: 378 5212 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 4737 1678 Size: 3059 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373 Size: 32 135 32 2659 32 42 32 2626 Fragments arranged by size: 2659 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2934 3180 669 Size: 988 281 996 246 3079 Fragments arranged by size: 3079 996 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5590 HpaI GTT'AAC Cuts at: 3180 3180 Size: 5590 KpnI G_GTAC'C Cuts at: 2915 2915 Size: 5590 MfeI C'AATT_G Cuts at: 3189 3189 Size: 5590 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4704 4888 1305 Size: 3399 184 2007 Fragments arranged by size: 3399 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2246 10 Size: 54 1425 343 414 3354 Fragments arranged by size: 3354 1425 414 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2321 2327 2579 2870 3548 3907 Size: 648 1026 129 6 252 291 678 359 Cuts at: 3907 4066 518 Size: 159 2042 Fragments arranged by size: 2042 1026 678 648 359 291 252 159 129 6 MspA1I CmG'CkG Cuts at: 1606 2242 2305 2618 3006 3683 4831 5076 1606 Size: 636 63 313 388 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 636 388 313 245 63 NcoI C'CATG_G Cuts at: 513 1065 2004 2607 513 Size: 552 939 603 3496 Fragments arranged by size: 3496 939 603 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5590 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5590 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NspI r_CATG'y Cuts at: 1132 1229 2326 2948 4493 1132 Size: 97 1097 622 1545 2229 Fragments arranged by size: 2229 1545 1097 622 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5590 PciI A'CATG_T Cuts at: 4489 4489 Size: 5590 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5590 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2001 2033 2481 2513 3659 3691 5172 5204 2001 Size: 32 448 32 1146 32 1481 32 2387 Fragments arranged by size: 2387 1481 1146 448 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2158 2158 Size: 5590 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5590 PsiI TTA'TAA Cuts at: 3160 3160 Size: 5590 PstI C_TGCA'G Cuts at: 830 2304 2942 830 Size: 1474 638 3478 Fragments arranged by size: 3478 1474 638 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5590 PvuII CAG'CTG Cuts at: 2305 2305 Size: 5590 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 3838 5550 3838 Size: 1712 3878 SfcI C'TryA_G Cuts at: 826 1045 2300 2938 4073 4754 4945 826 Size: 219 1255 638 1135 681 191 1471 Fragments arranged by size: 1471 1255 1135 681 638 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2917 1872 Size: 1045 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2447 3694 4595 4857 820 Size: 197 926 504 1247 901 262 1553 Fragments arranged by size: 1553 1247 926 901 504 262 197 SnaBI TAC'GTA Cuts at: 493 3381 493 Size: 2888 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2948 1132 Size: 1816 3774 SspI AAT'ATT Cuts at: 5 52 3514 5 Size: 47 3462 2081 Fragments arranged by size: 3462 2081 47 StuI AGG'CCT Cuts at: 1517 2646 1517 Size: 1129 4461 StyI C'CwwG_G Cuts at: 513 1065 2004 2607 2808 3029 513 Size: 552 939 603 201 221 3074 Fragments arranged by size: 3074 939 603 552 221 201 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2878 3617 3634 3787 3972 4417 325 Size: 1255 1298 739 17 153 185 445 1498 Fragments arranged by size: 1498 1298 1255 739 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3836 5548 96 Size: 274 80 33 51 1443 1859 1712 138 Fragments arranged by size: 1859 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2204 2236 2483 2515 5550 5582 2204 Size: 32 247 32 3035 32 2212 Fragments arranged by size: 3035 2212 247 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5590 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2194 2254 2194 Size: 60 5530 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5590 XmaI C'CCGG_G Cuts at: 1870 2915 1870 Size: 1045 4545 XmnI GAAnn'nnTTC Cuts at: 3719 3719 Size: 5590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3398 276 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI