Restriction map for pFN21AB5663
 (Circular) (Six-base) MAPSORT of: pFN21AB5663.seq  Check: 3199  from: 1  to: 5590



pFN21AB5663  5590 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:25  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3400     278 
   Size:         53      83     186     936    1864    2468

  Fragments arranged by size: 

                2468    1864     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2933    1937 
   Size:        996    4594
 
Acc65I G'GTAC_C

Cuts at:   2911    2911 
   Size:       5590
 
AclI AA'CG_TT

Cuts at:   1563    3717    4090    1563 
   Size:       2154     373    3063

  Fragments arranged by size: 

                3063    2154     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2120    3653    5037    5305    1632 
   Size:        488    1533    1384     268    1917

  Fragments arranged by size: 

                1917    1533    1384     488     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5393
 
AflIII A'CryG_T

Cuts at:   1203    4489    5466    1203 
   Size:       3286     977    1327

  Fragments arranged by size: 

                3286    1327     977
 
AgeI A'CCGG_T

Cuts at:   4421    4421 
   Size:       5590
 
AhdI GACnn_n'nnGTC

Cuts at:   4319    4319 
   Size:       5590
 
AleI CACnn'nnGTG

Cuts at:   1166    2579    2870    1166 
   Size:       1413     291    3886

  Fragments arranged by size: 

                3886    1413     291
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5590
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2001    2033    2001 
   Size:         32    5558
 
AlwNI CAG_nnn'CTG

Cuts at:   2020    2308    3333    4905    2020 
   Size:        288    1025    1572    2705

  Fragments arranged by size: 

                2705    1572    1025     288
 
ApaLI G'TGCA_C

Cuts at:   3647    4803    3647 
   Size:       1156    4434
 
ApoI r'AATT_y

Cuts at:   1493    2899    3125    3277    5386    1493 
   Size:       1406     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1406     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4144     160 
   Size:        872    3112    1606

  Fragments arranged by size: 

                3112    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5590
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2405    2915    1870 
   Size:         73     462     510    4545

  Fragments arranged by size: 

                4545     510     462      73
 
BamHI G'GATC_C

Cuts at:   2920    3287    2920 
   Size:        367    5223
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2911    4366     618 
   Size:        325     217     627    1124    1455    1842

  Fragments arranged by size: 

                1842    1455    1124     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2283    2663    2909    1391 
   Size:        892     380     246    4072

  Fragments arranged by size: 

                4072     892     380     246
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2227     928 
   Size:        971     328    4291

  Fragments arranged by size: 

                4291     971     328
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3781    3815    1903 
   Size:         34    1844      34    3678

  Fragments arranged by size: 

                3678    1844      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3482    4692    3482 
   Size:       1210    4380
 
BclI T'GATC_A

Cuts at:   1548    2712    1548 
   Size:       1164    4426
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4201     136 
   Size:        107     122      71     631     605    2529    1525

  Fragments arranged by size: 

                2529    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5585    5585 
   Size:       5590
 
BlpI GC'TnA_GC

Cuts at:   3007    3007 
   Size:       5590
 
Bme1580I G_kGCm'C

Cuts at:   1790    3651    4807    1790 
   Size:       1861    1156    2573

  Fragments arranged by size: 

                2573    1861    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2872    2881    1318 
   Size:       1554       9    4027

  Fragments arranged by size: 

                4027    1554       9
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3321    4279     442 
   Size:        416      53      83     399      24    1904     958    1753

  Fragments arranged by size: 

                1904    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5590
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2219    2297    2689    2789    4250    1239 
   Size:        980      78     392     100    1461    2579

  Fragments arranged by size: 

                2579    1461     980     392     100      78
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2432    3715    4580    4878    2432 
   Size:       1283     865     298    3144

  Fragments arranged by size: 

                3144    1283     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2105    2342    4253     882 
   Size:        617     606     237    1911    2219

  Fragments arranged by size: 

                2219    1911     617     606     237
 
BsaAI yAC'GTr

Cuts at:    493    1204    3381     493 
   Size:        711    2177    2702

  Fragments arranged by size: 

                2702    2177     711
 
BsaBI GATnn'nnATC

Cuts at:   5584    5584 
   Size:       5590
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3397    3779    4414     275 
   Size:         53      83     186     936    1864     382     635    1451

  Fragments arranged by size: 

                1864    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4023    4421    4695    4842    5253    4023 
   Size:        398     274     147     411    4360

  Fragments arranged by size: 

                4360     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2001    2031    2483    2513    1178 
   Size:         30     165      30     598      30     452      30    4255

  Fragments arranged by size: 

                4255     598     452     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2060    2123    2240    2243    2423    2534    2671    2834 
   Size:        874      63     117       3     180     111     137     163

Cuts at:   2834    2837    2846    2849    2852    2855    1186 
   Size:          3       9       3       3       3    3921

  Fragments arranged by size: 

                3921     874     180     163     137     117     111      63
                   9       3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:   2308    2790    4793    5289    2308 
   Size:        482    2003     496    2609

  Fragments arranged by size: 

                2609    2003     496     482
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2168    1163 
   Size:        573     432    4585

  Fragments arranged by size: 

                4585     573     432
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2080    2367    3040    3801    4829    1540 
   Size:        275     186      79     287     673     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     673     287     275     186      79
            
 
BsiHKAI G_wGCw'C

Cuts at:   3651    3736    4807    3651 
   Size:         85    1071    4434

  Fragments arranged by size: 

                4434    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3101    3194    3101 
   Size:         93    5497
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2283    2663    2909    3651    3736    4807    1391 
   Size:        399     493     380     246     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     493     399     380     246      85
            
 
BspEI T'CCGG_A

Cuts at:   5253    5253 
   Size:       5590
 
BspHI T'CATG_A

Cuts at:   3479    5209    3479 
   Size:       1730    3860
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2123    2945     844 
   Size:       1279     822    3489

  Fragments arranged by size: 

                3489    1279     822
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3477    1394 
   Size:        429    1654    3507

  Fragments arranged by size: 

                3507    1654     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2472    4085    4259      65 
   Size:       1183    1224    1613     174    1396

  Fragments arranged by size: 

                1613    1396    1224    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4234    4421    1700 
   Size:        110    2424     187    2869

  Fragments arranged by size: 

                2869    2424     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5590
 
BssHII G'CGCG_C

Cuts at:   1925    2118    1925 
   Size:        193    5397
 
BssSI C'ACGA_G

Cuts at:   3650    4662    3650 
   Size:       1012    4578
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5590
 
BstBI TT'CG_AA

Cuts at:   4394    4394 
   Size:       5590
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5590
 
BstYI r'GATC_y

Cuts at:   1972    2920    3287    3672    3689    5141    5227    5239    5585 
   Size:        948     367     385      17    1452      86      12     346

Cuts at:   5585    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     948     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2654    2654 
   Size:       5590
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2607     513 
   Size:        552     939     603    3496

  Fragments arranged by size: 

                3496     939     603     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4851

  Fragments arranged by size: 

                4851     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2643    3106    3900    3920    2643 
   Size:        463     794      20    4313

  Fragments arranged by size: 

                4313     794     463      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5590
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5555
 
DraI TTT'AAA

Cuts at:   2894    2894 
   Size:       5590
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4597     809 
   Size:        507    3281    1802

  Fragments arranged by size: 

                3281    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2244    2364    2460 
   Size:         54    1425      50     275      18     414     120      96

Cuts at:   2460    2631    3037    3926    5555       8 
   Size:        171     406     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     414     406     275     171     120
                  96      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2364    3037    1537 
   Size:        275     552     673    4090

  Fragments arranged by size: 

                4090     673     552     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2823    3520    1353 
   Size:       1470     697    3423

  Fragments arranged by size: 

                3423    1470     697
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2033    2120    2531    2589    4177    4551 
   Size:       1064     138     444      87     411      58    1588     374

Cuts at:   4551    4697     387 
   Size:        146    1280

  Fragments arranged by size: 

                1588    1280    1064     444     411     374     146     138
                  87      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2120    2219    2297    2689    2789    3653    4250 
   Size:        393     488      99      78     392     100     864     597

Cuts at:   4250    5037    5305    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     864     787     597     488     393     392     268
                 100      99      78
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2783    2783 
   Size:       5590
 
EcoO109I rG'GnC_Cy

Cuts at:   2158    2158 
   Size:       5590
 
EcoRI G'AATT_C

Cuts at:   2899    3277    2899 
   Size:        378    5212
 
FspI TGC'GCA

Cuts at:   4096    4096 
   Size:       5590
 
HaeII r_GCGC'y

Cuts at:   1678    4737    1678 
   Size:       3059    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4231    4263    4305    4337    1373 
   Size:         32     135      32    2659      32      42      32    2626

  Fragments arranged by size: 

                2659    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2934    3180     669 
   Size:        988     281     996     246    3079

  Fragments arranged by size: 

                3079     996     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5590
 
HpaI GTT'AAC

Cuts at:   3180    3180 
   Size:       5590
 
KpnI G_GTAC'C

Cuts at:   2915    2915 
   Size:       5590
 
MfeI C'AATT_G

Cuts at:   3189    3189 
   Size:       5590
 
MluI A'CGCG_T

Cuts at:   5466    5466 
   Size:       5590
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4704    4888    1305 
   Size:       3399     184    2007

  Fragments arranged by size: 

                3399    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2246      10 
   Size:         54    1425     343     414    3354

  Fragments arranged by size: 

                3354    1425     414     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2192    2321    2327    2579    2870    3548    3907 
   Size:        648    1026     129       6     252     291     678     359

Cuts at:   3907    4066     518 
   Size:        159    2042

  Fragments arranged by size: 

                2042    1026     678     648     359     291     252     159
                 129       6
 
MspA1I CmG'CkG

Cuts at:   1606    2242    2305    2618    3006    3683    4831    5076    1606 
   Size:        636      63     313     388     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     636     388     313     245      63
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2607     513 
   Size:        552     939     603    3496

  Fragments arranged by size: 

                3496     939     603     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5590
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5590
 
NotI GC'GGCC_GC

Cuts at:   3037    3037 
   Size:       5590
 
NspI r_CATG'y

Cuts at:   1132    1229    2326    2948    4493    1132 
   Size:         97    1097     622    1545    2229

  Fragments arranged by size: 

                2229    1545    1097     622      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5590
 
PciI A'CATG_T

Cuts at:   4489    4489 
   Size:       5590
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5590
 
PmeI GTTT'AAAC

Cuts at:   2894    2894 
   Size:       5590
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2001    2033    2481    2513    3659    3691    5172    5204    2001 
   Size:         32     448      32    1146      32    1481      32    2387

  Fragments arranged by size: 

                2387    1481    1146     448      32      32      32      32
            
 
PpuMI rG'GwC_Cy

Cuts at:   2158    2158 
   Size:       5590
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5590
 
PsiI TTA'TAA

Cuts at:   3160    3160 
   Size:       5590
 
PstI C_TGCA'G

Cuts at:    830    2304    2942     830 
   Size:       1474     638    3478

  Fragments arranged by size: 

                3478    1474     638
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5590
 
PvuII CAG'CTG

Cuts at:   2305    2305 
   Size:       5590
 
SalI G'TCGA_C

Cuts at:   1936    2932    1936 
   Size:        996    4594
 
SbfI CC_TGCA'GG

Cuts at:   2942    2942 
   Size:       5590
 
ScaI AGT'ACT

Cuts at:   3838    5550    3838 
   Size:       1712    3878
 
SfcI C'TryA_G

Cuts at:    826    1045    2300    2938    4073    4754    4945     826 
   Size:        219    1255     638    1135     681     191    1471

  Fragments arranged by size: 

                1471    1255    1135     681     638     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5590
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5590
 
SmaI CCC'GGG

Cuts at:   1872    2917    1872 
   Size:       1045    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2447    3694    4595    4857     820 
   Size:        197     926     504    1247     901     262    1553

  Fragments arranged by size: 

                1553    1247     926     901     504     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3381     493 
   Size:       2888    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5590
 
SphI G_CATG'C

Cuts at:   1132    2948    1132 
   Size:       1816    3774
 
SspI AAT'ATT

Cuts at:      5      52    3514       5 
   Size:         47    3462    2081

  Fragments arranged by size: 

                3462    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2646    1517 
   Size:       1129    4461
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2607    2808    3029     513 
   Size:        552     939     603     201     221    3074

  Fragments arranged by size: 

                3074     939     603     552     221     201
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2878    3617    3634    3787    3972    4417     325 
   Size:       1255    1298     739      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1298    1255     739     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3836    5548      96 
   Size:        274      80      33      51    1443    1859    1712     138

  Fragments arranged by size: 

                1859    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2204    2236    2483    2515    5550    5582    2204 
   Size:         32     247      32    3035      32    2212

  Fragments arranged by size: 

                3035    2212     247      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5590
 
XbaI T'CTAG_A

Cuts at:   2926    2926 
   Size:       5590
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2194    2254    2194 
   Size:         60    5530
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5590
 
XmaI C'CCGG_G

Cuts at:   1870    2915    1870 
   Size:       1045    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3719    3719 
   Size:       5590
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3398     276 
   Size:         53      83     186     936    1864    2468

  Fragments arranged by size: 

                2468    1864     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   DraIII
  EcoICRI    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI
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