(Circular) (Six-base) MAPSORT of: pFN21AB5640.seq Check: 3642 from: 1 to: 6241 pFN21AB5640 6241 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4051 278 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2982 3584 1937 Size: 1045 602 4594 Fragments arranged by size: 4594 1045 602 Acc65I G'GTAC_C Cuts at: 3562 3562 Size: 6241 AclI AA'CG_TT Cuts at: 1563 4368 4741 1563 Size: 2805 373 3063 Fragments arranged by size: 3063 2805 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2633 2846 3284 3380 4304 5688 5956 1632 Size: 1001 213 438 96 924 1384 268 1917 Fragments arranged by size: 1917 1384 1001 924 438 268 213 96 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6044 AflIII A'CryG_T Cuts at: 1203 2156 5140 6117 1203 Size: 953 2984 977 1327 Fragments arranged by size: 2984 1327 977 953 AgeI A'CCGG_T Cuts at: 5072 5072 Size: 6241 AhdI GACnn_n'nnGTC Cuts at: 4970 4970 Size: 6241 AleI CACnn'nnGTG Cuts at: 1166 3500 1166 Size: 2334 3907 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6241 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2078 2110 2078 Size: 32 6209 AlwNI CAG_nnn'CTG Cuts at: 3984 5556 3984 Size: 1572 4669 ApaI G_GGCC'C Cuts at: 2718 2718 Size: 6241 ApaLI G'TGCA_C Cuts at: 4298 5454 4298 Size: 1156 5085 ApoI r'AATT_y Cuts at: 1493 3550 3776 3928 6037 1493 Size: 2057 226 152 2109 1697 Fragments arranged by size: 2109 2057 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4795 160 Size: 872 3763 1606 Fragments arranged by size: 3763 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 AvaI C'yCGr_G Cuts at: 1870 1943 2172 3566 1870 Size: 73 229 1394 4545 Fragments arranged by size: 4545 1394 229 73 BamHI G'GATC_C Cuts at: 3571 3938 3571 Size: 367 5874 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2268 2578 3562 5017 618 Size: 325 217 627 481 310 984 1455 1842 Fragments arranged by size: 1842 1455 984 627 481 325 310 217 BanII G_rGCy'C Cuts at: 1391 2261 2514 2718 3155 3177 3560 1391 Size: 870 253 204 437 22 383 4072 Fragments arranged by size: 4072 870 437 383 253 204 22 BbeI G_GCGC'C Cuts at: 2272 2272 Size: 6241 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3171 3187 928 Size: 971 1272 16 3982 Fragments arranged by size: 3982 1272 971 16 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4432 4466 1903 Size: 34 2495 34 3678 Fragments arranged by size: 3678 2495 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2359 4133 5343 2359 Size: 1774 1210 3257 Fragments arranged by size: 3257 1774 1210 BclI T'GATC_A Cuts at: 1548 2661 3076 1548 Size: 1113 415 4713 Fragments arranged by size: 4713 1113 415 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2345 4852 136 Size: 107 122 71 631 605 673 2507 1525 Fragments arranged by size: 2507 1525 673 631 605 122 107 71 BglII A'GATC_T Cuts at: 6236 6236 Size: 6241 BlpI GC'TnA_GC Cuts at: 3154 3658 3154 Size: 504 5737 Bme1580I G_kGCm'C Cuts at: 1790 2718 2911 4302 5458 1790 Size: 928 193 1391 1156 2573 Fragments arranged by size: 2573 1391 1156 928 193 BmgBI CAC'GTC Cuts at: 1318 2215 1318 Size: 897 5344 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3972 4930 442 Size: 416 53 83 399 24 2555 958 1753 Fragments arranged by size: 2555 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2375 2426 2667 2861 2941 4901 1239 Size: 866 270 51 241 194 80 1960 2579 Fragments arranged by size: 2579 1960 866 270 241 194 80 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4366 5231 5529 4366 Size: 865 298 5078 Fragments arranged by size: 5078 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3030 4904 882 Size: 617 1531 1874 2219 Fragments arranged by size: 2219 1874 1531 617 BsaAI yAC'GTr Cuts at: 493 1204 4032 493 Size: 711 2828 2702 Fragments arranged by size: 2828 2702 711 BsaBI GATnn'nnATC Cuts at: 2108 6235 2108 Size: 4127 2114 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2269 4048 4430 5065 Size: 53 83 186 936 736 1779 382 635 Cuts at: 5065 275 Size: 1451 Fragments arranged by size: 1779 1451 936 736 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4674 5072 5346 5493 5904 4674 Size: 398 274 147 411 5011 Fragments arranged by size: 5011 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2108 1178 Size: 30 165 30 675 30 5311 Fragments arranged by size: 5311 675 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2987 3248 1186 Size: 1801 261 4179 Fragments arranged by size: 4179 1801 261 BseYI C'CCAG_C Cuts at: 2845 5444 5940 2845 Size: 2599 496 3146 Fragments arranged by size: 3146 2599 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2220 2288 1163 Size: 573 484 68 5116 Fragments arranged by size: 5116 573 484 68 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3691 4452 5480 1540 Size: 275 186 1690 761 1028 2301 Fragments arranged by size: 2301 1690 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4302 4387 5458 4302 Size: 85 1071 5085 Fragments arranged by size: 5085 1071 85 BsmI GAATG_Cn' Cuts at: 3149 3752 3845 3149 Size: 603 93 5545 Fragments arranged by size: 5545 603 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2351 2351 Size: 6241 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2261 2514 2718 2911 3155 3177 3560 Size: 399 471 253 204 193 244 22 383 Cuts at: 3560 4302 4387 5458 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 471 399 383 253 244 204 193 85 22 BspEI T'CCGG_A Cuts at: 5904 5904 Size: 6241 BspHI T'CATG_A Cuts at: 4130 5860 4130 Size: 1730 4511 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2968 3596 844 Size: 2124 628 3489 Fragments arranged by size: 3489 2124 628 BsrBI CCG'CTC Cuts at: 1394 1823 4128 1394 Size: 429 2305 3507 Fragments arranged by size: 3507 2305 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4736 4910 65 Size: 1183 3488 174 1396 Fragments arranged by size: 3488 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2056 2271 2771 4885 5072 1700 Size: 110 246 215 500 2114 187 2869 Fragments arranged by size: 2869 2114 500 246 215 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6241 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6241 BssSI C'ACGA_G Cuts at: 4301 5313 4301 Size: 1012 5229 BstAPI GCAn_nnn'nTGC Cuts at: 794 2243 794 Size: 1449 4792 BstBI TT'CG_AA Cuts at: 5045 5045 Size: 6241 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6241 BstYI r'GATC_y Cuts at: 1972 3571 3938 4323 4340 5792 5878 5890 6236 Size: 1599 367 385 17 1452 86 12 346 Cuts at: 6236 1972 Size: 1977 Fragments arranged by size: 1977 1599 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2025 2264 2540 2604 3030 3159 Size: 552 939 21 239 276 64 426 129 Cuts at: 3159 513 Size: 3595 Fragments arranged by size: 3595 939 552 426 276 239 129 64 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5502 Fragments arranged by size: 5502 683 56 BtsI GCAGTG_nn' Cuts at: 2904 3757 4551 4571 2904 Size: 853 794 20 4574 Fragments arranged by size: 4574 853 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6241 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2767 2802 529 Size: 35 2203 35 3968 Fragments arranged by size: 3968 2203 35 35 DraI TTT'AAA Cuts at: 3445 3545 3445 Size: 100 6141 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5248 809 Size: 507 3932 1802 Fragments arranged by size: 3932 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2346 3486 Size: 54 1425 50 275 18 177 339 1140 Cuts at: 3486 3688 4577 6206 8 Size: 202 889 1629 43 Fragments arranged by size: 1629 1425 1140 889 339 275 202 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3688 1537 Size: 275 1876 4090 Fragments arranged by size: 4090 1876 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3182 4171 1353 Size: 1829 989 3423 Fragments arranged by size: 3423 1829 989 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3016 4828 5202 5348 387 Size: 1064 138 1427 1812 374 146 1280 Fragments arranged by size: 1812 1427 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2375 2426 2633 2667 2846 2861 Size: 393 473 270 51 207 34 179 15 Cuts at: 2861 2941 3284 3380 4304 4901 5688 5956 1239 Size: 80 343 96 924 597 787 268 1524 Fragments arranged by size: 1524 924 787 597 473 393 343 270 268 207 179 96 80 51 34 15 EcoNI CCTnn'n_nnAGG Cuts at: 2251 2251 Size: 6241 EcoO109I rG'GnC_Cy Cuts at: 3065 3065 Size: 6241 EcoRI G'AATT_C Cuts at: 3550 3928 3550 Size: 378 5863 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2782 3193 2782 Size: 411 5830 FspI TGC'GCA Cuts at: 4747 4747 Size: 6241 HaeII r_GCGC'y Cuts at: 1678 2272 5388 1678 Size: 594 3116 2531 Fragments arranged by size: 3116 2531 594 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4882 4914 4956 4988 1373 Size: 32 135 32 3310 32 42 32 2626 Fragments arranged by size: 3310 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3585 3831 669 Size: 988 281 1647 246 3079 Fragments arranged by size: 3079 1647 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6241 HpaI GTT'AAC Cuts at: 3831 3831 Size: 6241 KasI G'GCGC_C Cuts at: 2268 2268 Size: 6241 KpnI G_GTAC'C Cuts at: 3566 3566 Size: 6241 MfeI C'AATT_G Cuts at: 3840 3840 Size: 6241 MluI A'CGCG_T Cuts at: 6117 6117 Size: 6241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2767 5355 5539 1305 Size: 1462 2588 184 2007 Fragments arranged by size: 2588 2007 1462 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4242 Fragments arranged by size: 4242 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3500 4199 4558 4717 518 Size: 648 2334 699 359 159 2042 Fragments arranged by size: 2334 2042 699 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2311 3657 4334 5482 5727 1606 Size: 705 1346 677 1148 245 2120 Fragments arranged by size: 2120 1346 1148 705 677 245 NaeI GCC'GGC Cuts at: 2058 2273 2058 Size: 215 6026 NarI GG'CG_CC Cuts at: 2269 2269 Size: 6241 NcoI C'CATG_G Cuts at: 513 1065 2004 2264 2540 2604 3159 513 Size: 552 939 260 276 64 555 3595 Fragments arranged by size: 3595 939 555 552 276 260 64 NdeI CA'TA_TG Cuts at: 387 387 Size: 6241 NgoMIV G'CCGG_C Cuts at: 2056 2271 2056 Size: 215 6026 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6241 NotI GC'GGCC_GC Cuts at: 3688 3688 Size: 6241 NspI r_CATG'y Cuts at: 1132 1229 2160 3220 3599 5144 1132 Size: 97 931 1060 379 1545 2229 Fragments arranged by size: 2229 1545 1060 931 379 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6241 PciI A'CATG_T Cuts at: 2156 5140 2156 Size: 2984 3257 PflMI CCAn_nnn'nTGG Cuts at: 1108 3437 1108 Size: 2329 3912 PfoI T'CCnGG_A Cuts at: 2683 2813 2683 Size: 130 6111 PmeI GTTT'AAAC Cuts at: 3545 3545 Size: 6241 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2078 2110 2660 2692 4310 4342 5823 5855 2078 Size: 32 550 32 1618 32 1481 32 2464 Fragments arranged by size: 2464 1618 1481 550 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6241 PsiI TTA'TAA Cuts at: 3811 3811 Size: 6241 PspOMI G'GGCC_C Cuts at: 2714 2714 Size: 6241 PstI C_TGCA'G Cuts at: 830 3593 830 Size: 2763 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6241 PvuII CAG'CTG Cuts at: 2311 2311 Size: 6241 SalI G'TCGA_C Cuts at: 1936 3583 1936 Size: 1647 4594 SapI GCTCTTCn'nnn_ Cuts at: 3182 3182 Size: 6241 SbfI CC_TGCA'GG Cuts at: 3593 3593 Size: 6241 ScaI AGT'ACT Cuts at: 4489 6201 4489 Size: 1712 4529 SexAI A'CCwGG_T Cuts at: 2319 2319 Size: 6241 SfcI C'TryA_G Cuts at: 826 1045 3589 4724 5405 5596 826 Size: 219 2544 1135 681 191 1471 Fragments arranged by size: 2544 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2270 2270 Size: 6241 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6241 SmaI CCC'GGG Cuts at: 1872 3568 1872 Size: 1696 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4345 5246 5508 820 Size: 197 926 2402 901 262 1553 Fragments arranged by size: 2402 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4032 493 Size: 3539 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6241 SphI G_CATG'C Cuts at: 1132 3599 1132 Size: 2467 3774 SspI AAT'ATT Cuts at: 5 52 4165 5 Size: 47 4113 2081 Fragments arranged by size: 4113 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6241 StyI C'CwwG_G Cuts at: 513 1065 2004 2264 2343 2540 2604 2932 3159 Size: 552 939 260 79 197 64 328 227 Cuts at: 3159 3680 513 Size: 521 3074 Fragments arranged by size: 3074 939 552 521 328 260 227 197 79 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4268 4285 4438 4623 5068 325 Size: 1255 2688 17 153 185 445 1498 Fragments arranged by size: 2688 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4487 6199 96 Size: 274 80 33 51 1443 2510 1712 138 Fragments arranged by size: 2510 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6201 6233 6201 Size: 32 6209 Tth111I GACn'n_nGTC Cuts at: 1316 2486 1316 Size: 1170 5071 XbaI T'CTAG_A Cuts at: 3577 3577 Size: 6241 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2080 2965 2080 Size: 885 5356 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6241 XmaI C'CCGG_G Cuts at: 1870 3566 1870 Size: 1696 4545 XmnI GAAnn'nnTTC Cuts at: 4370 4370 Size: 6241 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4049 276 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI