Restriction map for
pFN21AB5640
(Linear) (Six-base) MAPSORT of: KIBB5640.seq Check: 4254 from: 1 to: 1536
KIBB5640.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 977 1536
Size: 977 559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 841 1279 1375 1536
Size: 628 213 438 96 161
Fragments arranged by size:
628 438 213 161 96
AflIII A'CryG_T
Cuts at: 0 151 1536
Size: 151 1385
AleI CACnn'nnGTG
Cuts at: 0 1495 1536
Size: 1495 41
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 73 105 1536
Size: 73 32 1431
Fragments arranged by size:
1431 73 32
ApaI G_GGCC'C
Cuts at: 0 713 1536
Size: 713 823
AvaI C'yCGr_G
Cuts at: 0 167 1536
Size: 167 1369
BanI G'GyrC_C
Cuts at: 0 263 573 1536
Size: 263 310 963
Fragments arranged by size:
963 310 263
BanII G_rGCy'C
Cuts at: 0 256 509 713 1150 1172 1536
Size: 256 253 204 437 22 364
Fragments arranged by size:
437 364 256 253 204 22
BbeI G_GCGC'C
Cuts at: 0 267 1536
Size: 267 1269
BbsI GAAGACnn'nnnn_
Cuts at: 0 1166 1182 1536
Size: 1166 16 354
Fragments arranged by size:
1166 354 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 354 1536
Size: 354 1182
BclI T'GATC_A
Cuts at: 0 656 1071 1536
Size: 656 415 465
Fragments arranged by size:
656 465 415
BglI GCCn_nnn'nGGC
Cuts at: 0 340 1536
Size: 340 1196
BlpI GC'TnA_GC
Cuts at: 0 1149 1536
Size: 1149 387
Bme1580I G_kGCm'C
Cuts at: 0 713 906 1536
Size: 713 193 630
Fragments arranged by size:
713 630 193
BmgBI CAC'GTC
Cuts at: 0 210 1536
Size: 210 1326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 370 421 662 856 936 1536
Size: 100 270 51 241 194 80 600
Fragments arranged by size:
600 270 241 194 100 80 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 1025 1536
Size: 1025 511
BsaBI GATnn'nnATC
Cuts at: 0 103 1536
Size: 103 1433
BsaHI Gr'CG_yC
Cuts at: 0 264 1536
Size: 264 1272
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 103 1536
Size: 73 30 1433
Fragments arranged by size:
1433 73 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 982 1243 1536
Size: 982 261 293
Fragments arranged by size:
982 293 261
BseYI C'CCAG_C
Cuts at: 0 840 1536
Size: 840 696
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 283 1536
Size: 215 68 1253
Fragments arranged by size:
1253 215 68
BsmI GAATG_Cn'
Cuts at: 0 1144 1536
Size: 1144 392
BsmBI CGTCTCn'nnnn_
Cuts at: 0 346 1536
Size: 346 1190
Bsp1286I G_dGCh'C
Cuts at: 0 256 509 713 906 1150 1172 1536
Size: 256 253 204 193 244 22 364
Fragments arranged by size:
364 256 253 244 204 193 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 963 1536
Size: 963 573
BsrFI r'CCGG_y
Cuts at: 0 51 266 766 1536
Size: 51 215 500 770
Fragments arranged by size:
770 500 215 51
BstAPI GCAn_nnn'nTGC
Cuts at: 0 238 1536
Size: 238 1298
BtgI C'CryG_G
Cuts at: 0 20 259 535 599 1025 1154 1536
Size: 20 239 276 64 426 129 382
Fragments arranged by size:
426 382 276 239 129 64 20
BtsI GCAGTG_nn'
Cuts at: 0 899 1536
Size: 899 637
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 762 797 1536
Size: 762 35 739
Fragments arranged by size:
762 739 35
DraI TTT'AAA
Cuts at: 0 1440 1536
Size: 1440 96
EaeI y'GGCC_r
Cuts at: 0 2 341 1481 1536
Size: 2 339 1140 55
Fragments arranged by size:
1140 339 55 2
EarI CTCTTCn'nnn_
Cuts at: 0 1177 1536
Size: 1177 359
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1011 1536
Size: 1011 525
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 370 421 628 662 841 856 936
Size: 100 270 51 207 34 179 15 80
Cuts at: 936 1279 1375 1536
Size: 343 96 161
Fragments arranged by size:
343 270 207 179 161 100 96 80
51 34 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 246 1536
Size: 246 1290
EcoO109I rG'GnC_Cy
Cuts at: 0 1060 1536
Size: 1060 476
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 777 1188 1536
Size: 777 411 348
Fragments arranged by size:
777 411 348
HaeII r_GCGC'y
Cuts at: 0 267 1536
Size: 267 1269
KasI G'GCGC_C
Cuts at: 0 263 1536
Size: 263 1273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 762 1536
Size: 762 774
MscI TGG'CCA
Cuts at: 0 4 1536
Size: 4 1532
MslI CAynn'nnrTG
Cuts at: 0 1495 1536
Size: 1495 41
MspA1I CmG'CkG
Cuts at: 0 306 1536
Size: 306 1230
NaeI GCC'GGC
Cuts at: 0 53 268 1536
Size: 53 215 1268
Fragments arranged by size:
1268 215 53
NarI GG'CG_CC
Cuts at: 0 264 1536
Size: 264 1272
NcoI C'CATG_G
Cuts at: 0 259 535 599 1154 1536
Size: 259 276 64 555 382
Fragments arranged by size:
555 382 276 259 64
NgoMIV G'CCGG_C
Cuts at: 0 51 266 1536
Size: 51 215 1270
Fragments arranged by size:
1270 215 51
NspI r_CATG'y
Cuts at: 0 155 1215 1536
Size: 155 1060 321
Fragments arranged by size:
1060 321 155
PciI A'CATG_T
Cuts at: 0 151 1536
Size: 151 1385
PflMI CCAn_nnn'nTGG
Cuts at: 0 1432 1536
Size: 1432 104
PfoI T'CCnGG_A
Cuts at: 0 678 808 1536
Size: 678 130 728
Fragments arranged by size:
728 678 130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 655 687 1536
Size: 73 32 550 32 849
Fragments arranged by size:
849 550 73 32 32
PspOMI G'GGCC_C
Cuts at: 0 709 1536
Size: 709 827
PvuII CAG'CTG
Cuts at: 0 306 1536
Size: 306 1230
SapI GCTCTTCn'nnn_
Cuts at: 0 1177 1536
Size: 1177 359
SexAI A'CCwGG_T
Cuts at: 0 314 1536
Size: 314 1222
SfoI GGC'GCC
Cuts at: 0 265 1536
Size: 265 1271
StyI C'CwwG_G
Cuts at: 0 259 338 535 599 927 1154 1536
Size: 259 79 197 64 328 227 382
Fragments arranged by size:
382 328 259 227 197 79 64
Tth111I GACn'n_nGTC
Cuts at: 0 481 1536
Size: 481 1055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 75 960 1536
Size: 75 885 576
Fragments arranged by size:
885 576 75
Enzymes that do cut:
AccI AcuI AflIII AleI AloI ApaI AvaI BanI
BanII BbeI BbsI BciVI BclI BglI BlpI Bme1580I
BmgBI BpmI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsmI BsmBI Bsp1286I BspMI BsrFI BstAPI BtgI
BtsI CspCI DraI EaeI EarI EciI Eco57MI EcoNI
EcoO109I FalI HaeII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PpiI PspOMI PvuII SapI SexAI SfoI StyI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BfrBI BglII BmrI BmtI
BplI Bpu10I BpuEI BsaAI BsaWI BsiEI BsiHKAI BsiWI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI DraIII
DrdI EagI EcoICRI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI XbaI XhoI
XmaI XmnI ZraI