Restriction map for
pFN21AB5634
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbrPI BcgI BfrI BlnI BsaAI
BsaBI BsiEI BsiWI Bsp1407I BspLU11I
BsrBI BsrDI BsrGI Bst1107I BstBI
BstZ17I Bsu36I CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI HincII HindII HpaI KpnI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1309 GTCTAC GT^[AC][GT]AC
AccIII 1871 TCCGGA T^CCGGA
AcsI 1543 AAATTC [GA]^AATT[CT]
AflIII 302 ACGTGT A^C[GA][CT]GT
AlwI 1911 GATCC GGATC(4/5)
ApaI 2101 GGGCCC GGGCC^C
ApoI 1543 AAATTC [GA]^AATT[CT]
AspHI 1984 GTGCTC G[AT]GC[AT]^C
AviII 2055 TGCGCA TGC^GCA
BbvCI 2226 CCTCAGC CCTCAGC(-5/-2)
BciVI 1216 GGATAC GTATCC(6/5)
BclI 1838 TGATCA T^GATCA
BfaI 1202 CTAG C^TAG
BglII 1496 AGATCT A^GATCT
BlpI 2311 GCTGAGC GC^T[ACGT]AGC
BpmI 391 CTGGAG CTGGAG(16/14)
Bpu1102I 2311 GCTGAGC GC^T[ACGT]AGC
BsaI 551 GAGACC GGTCTC(1/5)
BseAI 1871 TCCGGA T^CCGGA
BsiHKAI 1984 GTGCTC G[AT]GC[AT]^C
BsmBI 299 GAGACG CGTCTC(1/5)
BsmI 234 GAATGC GAATGC(1/-1)
BspDI 604 ATCGAT AT^CGAT
BspEI 1871 TCCGGA T^CCGGA
BsrFI 125 ACCGGC [GA]^CCGG[CT]
BssHII 1886 GCGCGC G^CGCGC
BssSI 1915 CACGAG CACGAG(-5/-1)
BstEII 1926 GGTCACC G^GT[ACGT]ACC
BstPI 1926 GGTCACC G^GT[ACGT]ACC
CelII 2311 GCTGAGC GC^T[ACGT]AGC
Cfr10I 125 ACCGGC [GA]^CCGG[CT]
ClaI 604 ATCGAT AT^CGAT
DrdI 1300 GACAGAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 172 GAAGAG CTCTTC(1/4)
EcoO65I 1926 GGTCACC G^GT[ACGT]ACC
FbaI 1838 TGATCA T^GATCA
FspI 2055 TGCGCA TGC^GCA
HindIII 639 AAGCTT A^AGCTT
Ksp632I 172 GAAGAG CTCTTC(1/4)
MaeI 1202 CTAG C^TAG
MroI 1871 TCCGGA T^CCGGA
PflMI 1794 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 501 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1492 CTGCAG CTGCA^G
SfcI 1492 CTGCAG C^T[GA][CT]AG
SgrAI 124 CACCGGCG C[GA]^CCGG[CT]G
Van91I 1794 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BalI 1181 TGGCCA TGG^CCA
1479 TGGCCA
BbeI 34 GGCGCC GGCGC^C
128 GGCGCC
BbsI 805 GAAGAC GAAGAC(2/6)
274 GTCTTC
BglI 2016 GCCCCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2070 GCCACCGAGGC
BpuAI 805 GAAGAC GAAGAC(2/6)
274 GTCTTC
BsaWI 869 ACCGGA [AT]^CCGG[AT]
1871 TCCGGA
BsgI 2182 CTGCAC GTGCAG(16/14)
2062 GTGCAG
BspHI 11 TCATGA T^CATGA
423 TCATGA
BstAPI 73 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
523 GCAGTGGATGC
BstXI 725 CCACCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1340 CCAAAGAACTGG
BstYI 1496 AGATCT [GA]^GATC[CT]
1910 AGATCC
HaeII 34 GGCGCC [GA]GCGC^[CT]
128 GGCGCC
KasI 34 GGCGCC G^GCGCC
128 GGCGCC
MflI 1496 AGATCT [GA]^GATC[CT]
1910 AGATCC
MluNI 1181 TGGCCA TGG^CCA
1479 TGGCCA
MscI 1181 TGGCCA TGG^CCA
1479 TGGCCA
MseI 2279 TTAA T^TAA
2300 TTAA
NarI 34 GGCGCC GG^CGCC
128 GGCGCC
NcoI 1184 CCATGG C^CATGG
1476 CCATGG
RcaI 11 TCATGA T^CATGA
423 TCATGA
SfoI 34 GGCGCC GGC^GCC
128 GGCGCC
SmaI 2104 CCCGGG CCC^GGG
2333 CCCGGG
TfiI 1351 GATTC G^A[AT]TC
1378 GAATC
Tru9I 2279 TTAA T^TAA
2300 TTAA
Tsp509I 610 AATT ^AATT
1544 AATT
XcmI 311 CCATTGAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
962 CCATGAAGCCTCTGG
XhoII 1496 AGATCT [GA]^GATC[CT]
1910 AGATCC
XmaCI 2104 CCCGGG C^CCGGG
2333 CCCGGG
XmaI 2104 CCCGGG C^CCGGG
2333 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KspI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI Tsp45I
TspRI TthHB8I