(Circular) (Six-base) MAPSORT of: pFN21AB5633.seq Check: 9771 from: 1 to: 6271 pFN21AB5633 6271 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4081 278 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3614 1937 Size: 1677 4594 Acc65I G'GTAC_C Cuts at: 3592 3592 Size: 6271 AclI AA'CG_TT Cuts at: 1563 4398 4771 1563 Size: 2835 373 3063 Fragments arranged by size: 3063 2835 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2561 3173 3339 4334 5718 5986 1632 Size: 929 612 166 995 1384 268 1917 Fragments arranged by size: 1917 1384 995 929 612 268 166 AfeI AGC'GCT Cuts at: 2089 3209 2089 Size: 1120 5151 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6074 AflIII A'CryG_T Cuts at: 1203 5170 6147 1203 Size: 3967 977 1327 Fragments arranged by size: 3967 1327 977 AgeI A'CCGG_T Cuts at: 5102 5102 Size: 6271 AhdI GACnn_n'nnGTC Cuts at: 5000 5000 Size: 6271 AleI CACnn'nnGTG Cuts at: 1166 3046 1166 Size: 1880 4391 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3064 3197 1184 Size: 1880 133 4258 Fragments arranged by size: 4258 1880 133 AlwNI CAG_nnn'CTG Cuts at: 2082 4014 5586 2082 Size: 1932 1572 2767 Fragments arranged by size: 2767 1932 1572 ApaLI G'TGCA_C Cuts at: 4328 5484 4328 Size: 1156 5115 ApoI r'AATT_y Cuts at: 1493 2516 2884 3057 3580 3806 3958 6067 1493 Size: 1023 368 173 523 226 152 2109 1697 Fragments arranged by size: 2109 1697 1023 523 368 226 173 152 AseI AT'TA_AT Cuts at: 160 1032 2526 4825 160 Size: 872 1494 2299 1606 Fragments arranged by size: 2299 1606 1494 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 AvaI C'yCGr_G Cuts at: 1870 1943 3158 3596 1870 Size: 73 1215 438 4545 Fragments arranged by size: 4545 1215 438 73 BamHI G'GATC_C Cuts at: 2036 3601 3968 2036 Size: 1565 367 4339 Fragments arranged by size: 4339 1565 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3592 5047 618 Size: 325 217 627 1805 1455 1842 Fragments arranged by size: 1842 1805 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2994 3590 1391 Size: 1603 596 4072 Fragments arranged by size: 4072 1603 596 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5300 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4462 4496 1903 Size: 34 2525 34 3678 Fragments arranged by size: 3678 2525 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4163 5373 4163 Size: 1210 5061 BclI T'GATC_A Cuts at: 1548 3310 1548 Size: 1762 4509 BfrBI ATG'CAT Cuts at: 3091 3436 3091 Size: 345 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4882 136 Size: 107 122 71 631 605 3210 1525 Fragments arranged by size: 3210 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6266 6266 Size: 6271 BlpI GC'TnA_GC Cuts at: 3204 3688 3204 Size: 484 5787 Bme1580I G_kGCm'C Cuts at: 1790 4332 5488 1790 Size: 2542 1156 2573 Fragments arranged by size: 2573 2542 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6271 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2797 4002 4960 Size: 416 53 83 399 24 1380 1205 958 Cuts at: 4960 442 Size: 1753 Fragments arranged by size: 1753 1380 1205 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6271 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2125 4931 1239 Size: 886 2806 2579 Fragments arranged by size: 2806 2579 886 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2468 3035 4396 5261 5559 2468 Size: 567 1361 865 298 3180 Fragments arranged by size: 3180 1361 865 567 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3505 4934 882 Size: 617 2006 1429 2219 Fragments arranged by size: 2219 2006 1429 617 BsaAI yAC'GTr Cuts at: 493 1204 4062 493 Size: 711 2858 2702 Fragments arranged by size: 2858 2702 711 BsaBI GATnn'nnATC Cuts at: 6265 6265 Size: 6271 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4078 4460 5095 275 Size: 53 83 186 936 2545 382 635 1451 Fragments arranged by size: 2545 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2880 4704 5102 5376 5523 5934 2880 Size: 1824 398 274 147 411 3217 Fragments arranged by size: 3217 1824 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3523 3553 1178 Size: 30 165 30 2120 30 3896 Fragments arranged by size: 3896 2120 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2171 3125 1186 Size: 985 954 4332 Fragments arranged by size: 4332 985 954 BseYI C'CCAG_C Cuts at: 5474 5970 5474 Size: 496 5775 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5698 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3721 4482 5510 1540 Size: 275 186 1720 761 1028 2301 Fragments arranged by size: 2301 1720 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2994 4332 4417 5488 2994 Size: 1338 85 1071 3777 Fragments arranged by size: 3777 1338 1071 85 BsmI GAATG_Cn' Cuts at: 2216 3093 3782 3875 2216 Size: 877 689 93 4612 Fragments arranged by size: 4612 877 689 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2994 3590 4332 4417 5488 1391 Size: 399 1204 596 742 85 1071 2174 Fragments arranged by size: 2174 1204 1071 742 596 399 85 BspEI T'CCGG_A Cuts at: 2880 5934 2880 Size: 3054 3217 BspHI T'CATG_A Cuts at: 4160 5890 4160 Size: 1730 4541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2297 3626 844 Size: 1453 1329 3489 Fragments arranged by size: 3489 1453 1329 BsrBI CCG'CTC Cuts at: 1394 1823 4158 1394 Size: 429 2335 3507 Fragments arranged by size: 3507 2335 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3058 4766 4940 65 Size: 1183 1810 1708 174 1396 Fragments arranged by size: 1810 1708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4915 5102 1700 Size: 110 3105 187 2869 Fragments arranged by size: 3105 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6271 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6271 BssSI C'ACGA_G Cuts at: 4331 5343 4331 Size: 1012 5259 BstAPI GCAn_nnn'nTGC Cuts at: 794 2280 794 Size: 1486 4785 BstBI TT'CG_AA Cuts at: 3007 5075 3007 Size: 2068 4203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6271 BstYI r'GATC_y Cuts at: 1972 2036 2160 2610 3601 3968 4353 4370 5822 Size: 64 124 450 991 367 385 17 1452 Cuts at: 5822 5908 5920 6266 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 991 450 385 367 346 124 86 64 17 12 BtgI C'CryG_G Cuts at: 513 1065 2070 3398 513 Size: 552 1005 1328 3386 Fragments arranged by size: 3386 1328 1005 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5532 Fragments arranged by size: 5532 683 56 BtsI GCAGTG_nn' Cuts at: 2250 3053 3787 4581 4601 2250 Size: 803 734 794 20 3920 Fragments arranged by size: 3920 803 794 734 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3254 3289 3493 3528 529 Size: 35 2690 35 204 35 3272 Fragments arranged by size: 3272 2690 204 35 35 35 DraI TTT'AAA Cuts at: 3575 3575 Size: 6271 DraIII CAC_nnn'GTG Cuts at: 2468 2468 Size: 6271 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5278 809 Size: 507 3962 1802 Fragments arranged by size: 3962 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2073 2260 Size: 54 1425 50 275 18 237 6 187 Cuts at: 2260 3718 4607 6236 8 Size: 1458 889 1629 43 Fragments arranged by size: 1629 1458 1425 889 275 237 187 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3718 1537 Size: 275 1906 4090 Fragments arranged by size: 4090 1906 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3102 3339 4201 1353 Size: 1749 237 862 3423 Fragments arranged by size: 3423 1749 862 237 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4858 5232 5378 387 Size: 1064 138 3269 374 146 1280 Fragments arranged by size: 3269 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2992 2992 Size: 6271 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2125 2561 3173 3339 4334 4931 5718 Size: 393 493 436 612 166 995 597 787 Cuts at: 5718 5986 1239 Size: 268 1524 Fragments arranged by size: 1524 995 787 612 597 493 436 393 268 166 EcoRI G'AATT_C Cuts at: 3580 3958 3580 Size: 378 5893 FspI TGC'GCA Cuts at: 4777 4777 Size: 6271 HaeII r_GCGC'y Cuts at: 1678 2091 3211 5418 1678 Size: 413 1120 2207 2531 Fragments arranged by size: 2531 2207 1120 413 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2374 2406 2674 2706 3378 Size: 32 135 32 802 32 268 32 672 Cuts at: 3378 3410 4912 4944 4986 5018 1373 Size: 32 1502 32 42 32 2626 Fragments arranged by size: 2626 1502 802 672 268 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2326 3615 3861 669 Size: 988 281 388 1289 246 3079 Fragments arranged by size: 3079 1289 988 388 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6271 HpaI GTT'AAC Cuts at: 3861 3861 Size: 6271 KpnI G_GTAC'C Cuts at: 3596 3596 Size: 6271 MfeI C'AATT_G Cuts at: 3870 3870 Size: 6271 MluI A'CGCG_T Cuts at: 6147 6147 Size: 6271 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5385 5569 1305 Size: 4080 184 2007 Fragments arranged by size: 4080 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2069 2075 10 Size: 54 1425 343 237 6 4206 Fragments arranged by size: 4206 1425 343 237 54 6 MslI CAynn'nnrTG Cuts at: 518 1166 2816 3046 4229 4588 4747 518 Size: 648 1650 230 1183 359 159 2042 Fragments arranged by size: 2042 1650 1183 648 359 230 159 MspA1I CmG'CkG Cuts at: 1606 2031 3687 4364 5512 5757 1606 Size: 425 1656 677 1148 245 2120 Fragments arranged by size: 2120 1656 1148 677 425 245 NcoI C'CATG_G Cuts at: 513 1065 2070 513 Size: 552 1005 4714 Fragments arranged by size: 4714 1005 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6271 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6271 NotI GC'GGCC_GC Cuts at: 3718 3718 Size: 6271 NsiI A_TGCA'T Cuts at: 3093 3438 3093 Size: 345 5926 NspI r_CATG'y Cuts at: 1132 1229 3178 3629 5174 1132 Size: 97 1949 451 1545 2229 Fragments arranged by size: 2229 1949 1545 451 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6271 PciI A'CATG_T Cuts at: 5170 5170 Size: 6271 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6271 PfoI T'CCnGG_A Cuts at: 2889 2943 2889 Size: 54 6217 PmeI GTTT'AAAC Cuts at: 3575 3575 Size: 6271 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4340 4372 5853 5885 4340 Size: 32 1481 32 4726 Fragments arranged by size: 4726 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6271 PsiI TTA'TAA Cuts at: 3841 3841 Size: 6271 PstI C_TGCA'G Cuts at: 830 3623 830 Size: 2793 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6271 PvuII CAG'CTG Cuts at: 2031 2031 Size: 6271 SacI G_AGCT'C Cuts at: 2994 2994 Size: 6271 SalI G'TCGA_C Cuts at: 1936 3613 1936 Size: 1677 4594 SbfI CC_TGCA'GG Cuts at: 3623 3623 Size: 6271 ScaI AGT'ACT Cuts at: 2740 3556 4519 6231 2740 Size: 816 963 1712 2780 Fragments arranged by size: 2780 1712 963 816 SfcI C'TryA_G Cuts at: 826 1045 2083 2712 3619 4754 5435 5626 826 Size: 219 1038 629 907 1135 681 191 1471 Fragments arranged by size: 1471 1135 1038 907 681 629 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6271 SmaI CCC'GGG Cuts at: 1872 3160 3598 1872 Size: 1288 438 4545 Fragments arranged by size: 4545 1288 438 SmlI C'TyrA_G Cuts at: 820 1017 1943 2447 3014 4375 5276 5538 820 Size: 197 926 504 567 1361 901 262 1553 Fragments arranged by size: 1553 1361 926 901 567 504 262 197 SnaBI TAC'GTA Cuts at: 493 4062 493 Size: 3569 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6271 SphI G_CATG'C Cuts at: 1132 3178 3629 1132 Size: 2046 451 3774 Fragments arranged by size: 3774 2046 451 SspI AAT'ATT Cuts at: 5 52 2530 4195 5 Size: 47 2478 1665 2081 Fragments arranged by size: 2478 2081 1665 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6271 StyI C'CwwG_G Cuts at: 513 1065 2070 2552 3272 3710 513 Size: 552 1005 482 720 438 3074 Fragments arranged by size: 3074 1005 720 552 482 438 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2102 2816 4298 4315 4468 4653 5098 Size: 1255 522 714 1482 17 153 185 445 Cuts at: 5098 325 Size: 1498 Fragments arranged by size: 1498 1482 1255 714 522 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2738 3554 4517 Size: 274 80 33 51 1443 761 816 963 Cuts at: 4517 6229 96 Size: 1712 138 Fragments arranged by size: 1712 1443 963 816 761 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6231 6263 6231 Size: 32 6239 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6271 XbaI T'CTAG_A Cuts at: 2701 3607 2701 Size: 906 5365 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2149 2224 2149 Size: 75 6196 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6271 XmaI C'CCGG_G Cuts at: 1870 3158 3596 1870 Size: 1288 438 4545 Fragments arranged by size: 4545 1288 438 XmnI GAAnn'nnTTC Cuts at: 2898 4400 2898 Size: 1502 4769 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4079 276 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI