Restriction map for
pFN21AB5633
(Linear) (Six-base) MAPSORT of: KIBB5633.seq Check: 2452 from: 1 to: 1566
KIBB5633.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1168 1334 1566
Size: 556 612 166 232
Fragments arranged by size:
612 556 232 166
AfeI AGC'GCT
Cuts at: 0 84 1204 1566
Size: 84 1120 362
Fragments arranged by size:
1120 362 84
AleI CACnn'nnGTG
Cuts at: 0 1041 1566
Size: 1041 525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1059 1192 1566
Size: 1059 133 374
Fragments arranged by size:
1059 374 133
AlwNI CAG_nnn'CTG
Cuts at: 0 77 1566
Size: 77 1489
ApoI r'AATT_y
Cuts at: 0 511 879 1052 1566
Size: 511 368 173 514
Fragments arranged by size:
514 511 368 173
AseI AT'TA_AT
Cuts at: 0 521 1566
Size: 521 1045
AvaI C'yCGr_G
Cuts at: 0 1153 1566
Size: 1153 413
BamHI G'GATC_C
Cuts at: 0 31 1566
Size: 31 1535
BanII G_rGCy'C
Cuts at: 0 989 1566
Size: 989 577
BclI T'GATC_A
Cuts at: 0 1305 1566
Size: 1305 261
BfrBI ATG'CAT
Cuts at: 0 1086 1431 1566
Size: 1086 345 135
Fragments arranged by size:
1086 345 135
BlpI GC'TnA_GC
Cuts at: 0 1199 1566
Size: 1199 367
BmrI ACTGGGnnnn_n'
Cuts at: 0 792 1566
Size: 792 774
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 1566
Size: 120 1446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1030 1566
Size: 463 567 536
Fragments arranged by size:
567 536 463
BsaI GGTCTCn'nnnn_
Cuts at: 0 1500 1566
Size: 1500 66
BsaWI w'CCGG_w
Cuts at: 0 875 1566
Size: 875 691
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1518 1548 1566
Size: 1518 30 18
Fragments arranged by size:
1518 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 1120 1566
Size: 166 954 446
Fragments arranged by size:
954 446 166
BsiHKAI G_wGCw'C
Cuts at: 0 989 1566
Size: 989 577
BsmI GAATG_Cn'
Cuts at: 0 211 1088 1566
Size: 211 877 478
Fragments arranged by size:
877 478 211
Bsp1286I G_dGCh'C
Cuts at: 0 989 1566
Size: 989 577
BspEI T'CCGG_A
Cuts at: 0 875 1566
Size: 875 691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 292 1566
Size: 292 1274
BsrDI GCAATG_nn'
Cuts at: 0 1053 1566
Size: 1053 513
BstAPI GCAn_nnn'nTGC
Cuts at: 0 275 1566
Size: 275 1291
BstBI TT'CG_AA
Cuts at: 0 1002 1566
Size: 1002 564
BstYI r'GATC_y
Cuts at: 0 31 155 605 1566
Size: 31 124 450 961
Fragments arranged by size:
961 450 124 31
BtgI C'CryG_G
Cuts at: 0 65 1393 1566
Size: 65 1328 173
Fragments arranged by size:
1328 173 65
BtsI GCAGTG_nn'
Cuts at: 0 245 1048 1566
Size: 245 803 518
Fragments arranged by size:
803 518 245
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1249 1284 1488 1523 1566
Size: 1249 35 204 35 43
Fragments arranged by size:
1249 204 43 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 463 1566
Size: 463 1103
EaeI y'GGCC_r
Cuts at: 0 62 68 255 1566
Size: 62 6 187 1311
Fragments arranged by size:
1311 187 62 6
EarI CTCTTCn'nnn_
Cuts at: 0 1097 1334 1566
Size: 1097 237 232
Fragments arranged by size:
1097 237 232
EcoICRI GAG'CTC
Cuts at: 0 987 1566
Size: 987 579
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 556 1168 1334 1566
Size: 120 436 612 166 232
Fragments arranged by size:
612 436 232 166 120
HaeII r_GCGC'y
Cuts at: 0 86 1206 1566
Size: 86 1120 360
Fragments arranged by size:
1120 360 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 369 401 669 701 1373 1405 1566
Size: 369 32 268 32 672 32 161
Fragments arranged by size:
672 369 268 161 32 32 32
HincII GTy'rAC
Cuts at: 0 321 1566
Size: 321 1245
MscI TGG'CCA
Cuts at: 0 64 70 1566
Size: 64 6 1496
Fragments arranged by size:
1496 64 6
MslI CAynn'nnrTG
Cuts at: 0 811 1041 1566
Size: 811 230 525
Fragments arranged by size:
811 525 230
MspA1I CmG'CkG
Cuts at: 0 26 1566
Size: 26 1540
NcoI C'CATG_G
Cuts at: 0 65 1566
Size: 65 1501
NsiI A_TGCA'T
Cuts at: 0 1088 1433 1566
Size: 1088 345 133
Fragments arranged by size:
1088 345 133
NspI r_CATG'y
Cuts at: 0 1173 1566
Size: 1173 393
PfoI T'CCnGG_A
Cuts at: 0 884 938 1566
Size: 884 54 628
Fragments arranged by size:
884 628 54
PvuII CAG'CTG
Cuts at: 0 26 1566
Size: 26 1540
SacI G_AGCT'C
Cuts at: 0 989 1566
Size: 989 577
ScaI AGT'ACT
Cuts at: 0 735 1551 1566
Size: 735 816 15
Fragments arranged by size:
816 735 15
SfcI C'TryA_G
Cuts at: 0 78 707 1566
Size: 78 629 859
Fragments arranged by size:
859 629 78
SmaI CCC'GGG
Cuts at: 0 1155 1566
Size: 1155 411
SmlI C'TyrA_G
Cuts at: 0 442 1009 1566
Size: 442 567 557
Fragments arranged by size:
567 557 442
SphI G_CATG'C
Cuts at: 0 1173 1566
Size: 1173 393
SspI AAT'ATT
Cuts at: 0 525 1566
Size: 525 1041
StyI C'CwwG_G
Cuts at: 0 65 547 1267 1566
Size: 65 482 720 299
Fragments arranged by size:
720 482 299 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 97 811 1566
Size: 97 714 755
Fragments arranged by size:
755 714 97
TatI w'GTAC_w
Cuts at: 0 733 1549 1566
Size: 733 816 17
Fragments arranged by size:
816 733 17
XbaI T'CTAG_A
Cuts at: 0 696 1566
Size: 696 870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 219 1566
Size: 144 75 1347
Fragments arranged by size:
1347 144 75
XmaI C'CCGG_G
Cuts at: 0 1153 1566
Size: 1153 413
XmnI GAAnn'nnTTC
Cuts at: 0 893 1566
Size: 893 673
Enzymes that do cut:
AcuI AfeI AleI AlfI AlwNI ApoI AseI AvaI
BamHI BanII BclI BfrBI BlpI BmrI BpmI BpuEI
BsaI BsaWI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrDI BstAPI BstBI BstYI BtgI BtsI CspCI
DraIII EaeI EarI EcoICRI Eco57MI HaeII Hin4I HincII
MscI MslI MspA1I NcoI NsiI NspI PfoI PvuII
SacI ScaI SfcI SmaI SmlI SphI SspI StyI
TaqII TatI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BanI BbeI BbsI BbvCI BcgI BciVI BglI BglII
Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BseYI BsgI BsiEI BsiWI BsmBI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI DraI DrdI EagI EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
StuI SwaI TstI Tth111I XhoI ZraI