Restriction map for
pFN21AB5599
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI BsoBI BspDI
BspEI BspLU11I BsrBI BsrDI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII Hin1I
HincII HindII HpaI KasI KspI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I StuI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 688 GGTACC G^GTACC
AseI 613 ATTAAT AT^TAAT
AsnI 613 ATTAAT AT^TAAT
Asp718I 688 GGTACC G^GTACC
AspHI 2359 GTGCTC G[AT]GC[AT]^C
BalI 1745 TGGCCA TGG^CCA
BanI 688 GGTACC G^G[CT][GA]CC
BciVI 2265 GGATAC GTATCC(6/5)
BsgI 683 GTGCAG GTGCAG(16/14)
BsiHKAI 2359 GTGCTC G[AT]GC[AT]^C
BsmFI 30 GTCCC GGGAC(10/14)
BssSI 1940 CACGAG CACGAG(-5/-1)
BstXI 2169 CCAGAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 49 GCGC GCG^C
DraII 28 GGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 989 CTCTTC CTCTTC(1/4)
EcoO109I 28 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 296 CCTTGG C^C[AT][AT]GG
FauI 115 GCGGG CCCGC(4/6)
HgaI 1861 GACGC GACGC(5/10)
HhaI 49 GCGC GCG^C
HinP1I 49 GCGC G^CGC
HindIII 151 AAGCTT A^AGCTT
KpnI 688 GGTACC GGTAC^C
Ksp632I 989 CTCTTC CTCTTC(1/4)
MaeII 284 ACGT A^CGT
MluNI 1745 TGGCCA TGG^CCA
MscI 1745 TGGCCA TGG^CCA
MspA1I 1930 CAGCTG C[AC]G^C[GT]G
NaeI 10 GCCGGC GCC^GGC
NgoMIV 10 GCCGGC G^CCGGC
PpuMI 28 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 613 ATTAAT AT^TAAT
PvuII 1930 CAGCTG CAG^CTG
ScaI 165 AGTACT AGT^ACT
SpeI 60 ACTAGT A^CTAGT
StyI 296 CCTTGG C^C[AT][AT]GG
SwaI 1295 ATTTAAAT ATTT^AAAT
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# TWO CUT ENZYMES ( 21 enzymes) #
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Asp700I 302 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1370 GAATGGATTC
BclI 180 TGATCA T^GATCA
2490 TGATCA
BpmI 833 CTGGAG CTGGAG(16/14)
2177 CTGGAG
BsaBI 2383 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2686 GATTCCAATC
BsiEI 15 CGGCCG CG[GA][CT]^CG
74 CGGCCG
BsmI 1026 GAATGC GAATGC(1/-1)
2172 GAATGC
BspHI 1113 TCATGA T^CATGA
1359 TCATGA
BsrFI 10 GCCGGC [GA]^CCGG[CT]
573 ACCGGC
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
573 ACCGGC
EagI 15 CGGCCG C^GGCCG
74 CGGCCG
EclXI 15 CGGCCG C^GGCCG
74 CGGCCG
Eco52I 15 CGGCCG C^GGCCG
74 CGGCCG
FbaI 180 TGATCA T^GATCA
2490 TGATCA
MamI 2383 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2686 GATTCCAATC
PflMI 1966 CCATTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2065 CCATCCAATGG
RcaI 1113 TCATGA T^CATGA
1359 TCATGA
SspI 738 AATATT AAT^ATT
823 AATATT
Tsp45I 281 GTGAC ^GT[GC]AC
814 GTGAC
Van91I 1966 CCATTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2065 CCATCCAATGG
XcmI 1733 CCAGAGATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1962 CCACCCATTTTTTGG
XmnI 302 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1370 GAATGGATTC
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EaeI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII