(Circular) (Six-base) MAPSORT of: pFN21AB5599.seq Check: 3317 from: 1 to: 7501 pFN21AB5599 7501 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3070 4247 3070 Size: 1177 6324 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5311 278 Size: 53 83 186 936 3775 2468 Fragments arranged by size: 3775 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4844 1937 Size: 2907 4594 Acc65I G'GTAC_C Cuts at: 2693 4822 2693 Size: 2129 5372 AclI AA'CG_TT Cuts at: 1563 5628 6001 1563 Size: 4065 373 3063 Fragments arranged by size: 4065 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3800 3891 5564 6948 7216 1632 Size: 2168 91 1673 1384 268 1917 Fragments arranged by size: 2168 1917 1673 1384 268 91 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7304 AflIII A'CryG_T Cuts at: 1203 6400 7377 1203 Size: 5197 977 1327 Fragments arranged by size: 5197 1327 977 AgeI A'CCGG_T Cuts at: 6332 6332 Size: 7501 AhdI GACnn_n'nnGTC Cuts at: 6230 6230 Size: 7501 AleI CACnn'nnGTG Cuts at: 1166 2108 2345 1166 Size: 942 237 6322 Fragments arranged by size: 6322 942 237 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7501 AlwNI CAG_nnn'CTG Cuts at: 2663 3937 4682 5244 6816 2663 Size: 1274 745 562 1572 3348 Fragments arranged by size: 3348 1572 1274 745 562 ApaLI G'TGCA_C Cuts at: 5558 6714 5558 Size: 1156 6345 ApoI r'AATT_y Cuts at: 1493 2309 2369 2708 3218 3298 4516 4525 4810 Size: 816 60 339 510 80 1218 9 285 Cuts at: 4810 5036 5188 7297 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1218 816 510 339 285 226 152 80 60 9 AseI AT'TA_AT Cuts at: 160 1032 2619 6055 160 Size: 872 1587 3436 1606 Fragments arranged by size: 3436 1606 1587 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7501 AvaI C'yCGr_G Cuts at: 1870 1943 4826 1870 Size: 73 2883 4545 Fragments arranged by size: 4545 2883 73 BamHI G'GATC_C Cuts at: 4831 5198 4831 Size: 367 7134 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2693 4822 6277 618 Size: 325 217 627 906 2129 1455 1842 Fragments arranged by size: 2129 1842 1455 906 627 325 217 BanII G_rGCy'C Cuts at: 1391 2133 3199 3648 4820 1391 Size: 742 1066 449 1172 4072 Fragments arranged by size: 4072 1172 1066 742 449 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3290 3444 4497 928 Size: 971 1391 154 1053 3932 Fragments arranged by size: 3932 1391 1053 971 154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5692 5726 1903 Size: 34 3755 34 3678 Fragments arranged by size: 3755 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4264 5393 6603 4264 Size: 1129 1210 5162 Fragments arranged by size: 5162 1210 1129 BclI T'GATC_A Cuts at: 1548 2185 4495 1548 Size: 637 2310 4554 Fragments arranged by size: 4554 2310 637 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6112 136 Size: 107 122 71 631 605 4440 1525 Fragments arranged by size: 4440 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7496 7496 Size: 7501 BlpI GC'TnA_GC Cuts at: 4918 4918 Size: 7501 Bme1580I G_kGCm'C Cuts at: 1790 2481 5562 6718 1790 Size: 691 3081 1156 2573 Fragments arranged by size: 3081 2573 1156 691 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7501 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3202 5232 6190 Size: 416 53 83 399 24 1785 2030 958 Cuts at: 6190 442 Size: 1753 Fragments arranged by size: 2030 1785 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7501 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2859 4203 6161 1239 Size: 1620 1344 1958 2579 Fragments arranged by size: 2579 1958 1620 1344 Bpu10I CC'TnA_GC Cuts at: 3762 3762 Size: 7501 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5626 6491 6789 5626 Size: 865 298 6338 Fragments arranged by size: 6338 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6164 882 Size: 617 4665 2219 Fragments arranged by size: 4665 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5292 493 Size: 711 4088 2702 Fragments arranged by size: 4088 2702 711 BsaBI GATnn'nnATC Cuts at: 4392 4695 7495 4392 Size: 303 2800 4398 Fragments arranged by size: 4398 2800 303 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5308 5690 6325 275 Size: 53 83 186 936 3775 382 635 1451 Fragments arranged by size: 3775 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5934 6332 6606 6753 7164 5934 Size: 398 274 147 411 6271 Fragments arranged by size: 6271 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2832 2862 4213 4243 1178 Size: 30 165 30 1429 30 1351 30 4436 Fragments arranged by size: 4436 1429 1351 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 3713 4358 4367 1186 Size: 973 1554 645 9 4320 Fragments arranged by size: 4320 1554 973 645 9 BseYI C'CCAG_C Cuts at: 2046 4710 6704 7200 2046 Size: 2664 1994 496 2347 Fragments arranged by size: 2664 2347 1994 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2709 1163 Size: 573 973 5955 Fragments arranged by size: 5955 973 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2023 2082 4951 5712 6740 1540 Size: 275 186 22 59 2869 761 1028 2301 Fragments arranged by size: 2869 2301 1028 761 275 186 59 22 BsiHKAI G_wGCw'C Cuts at: 4368 5562 5647 6718 4368 Size: 1194 85 1071 5151 Fragments arranged by size: 5151 1194 1071 85 BsmI GAATG_Cn' Cuts at: 3037 4183 5012 5105 3037 Size: 1146 829 93 5433 Fragments arranged by size: 5433 1146 829 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2481 3199 3648 4368 4820 5562 Size: 399 343 348 718 449 720 452 742 Cuts at: 5562 5647 6718 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 720 718 452 449 399 348 343 85 BspEI T'CCGG_A Cuts at: 7164 7164 Size: 7501 BspHI T'CATG_A Cuts at: 3118 3364 5390 7120 3118 Size: 246 2026 1730 3499 Fragments arranged by size: 3499 2026 1730 246 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2681 3070 3112 4247 4856 844 Size: 1837 389 42 1135 609 3489 Fragments arranged by size: 3489 1837 1135 609 389 42 BsrBI CCG'CTC Cuts at: 1394 1823 5388 1394 Size: 429 3565 3507 Fragments arranged by size: 3565 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5996 6170 65 Size: 1183 4748 174 1396 Fragments arranged by size: 4748 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2015 2578 6145 6332 1700 Size: 110 205 563 3567 187 2869 Fragments arranged by size: 3567 2869 563 205 187 110 BsrGI T'GTAC_A Cuts at: 96 2678 3952 4643 96 Size: 2582 1274 691 2954 Fragments arranged by size: 2954 2582 1274 691 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7501 BssSI C'ACGA_G Cuts at: 3945 5561 6573 3945 Size: 1616 1012 4873 Fragments arranged by size: 4873 1616 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7501 BstBI TT'CG_AA Cuts at: 6305 6305 Size: 7501 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7501 BstXI CCAn_nnnn'nTGG Cuts at: 4181 4181 Size: 7501 BstYI r'GATC_y Cuts at: 1972 3259 3600 4021 4287 4393 4831 5198 5583 Size: 1287 341 421 266 106 438 367 385 Cuts at: 5583 5600 7052 7138 7150 7496 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1287 438 421 385 367 346 341 266 106 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6949 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2071 487 Size: 683 56 845 5917 Fragments arranged by size: 5917 845 683 56 BtsI GCAGTG_nn' Cuts at: 2388 2528 4113 4234 5017 5811 5831 2388 Size: 140 1585 121 783 794 20 4058 Fragments arranged by size: 4058 1585 794 783 140 121 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7501 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7466 DraI TTT'AAA Cuts at: 2355 2625 3303 3406 4805 2355 Size: 270 678 103 1399 5051 Fragments arranged by size: 5051 1399 678 270 103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6508 809 Size: 507 5192 1802 Fragments arranged by size: 5192 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2020 2079 2100 Size: 54 1425 50 275 18 190 59 21 Cuts at: 2100 3750 4948 5837 7466 8 Size: 1650 1198 889 1629 43 Fragments arranged by size: 1650 1629 1425 1198 889 275 190 59 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2020 2079 4948 1537 Size: 275 208 59 2869 4090 Fragments arranged by size: 4090 2869 275 208 59 EarI CTCTTCn'nnn_ Cuts at: 1353 3000 5431 1353 Size: 1647 2431 3423 Fragments arranged by size: 3423 2431 1647 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6088 6462 6608 387 Size: 1064 138 4499 374 146 1280 Fragments arranged by size: 4499 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2859 3800 3891 4203 5564 6161 6948 Size: 393 1227 941 91 312 1361 597 787 Cuts at: 6948 7216 1239 Size: 268 1524 Fragments arranged by size: 1524 1361 1227 941 787 597 393 312 268 91 EcoO109I rG'GnC_Cy Cuts at: 2034 2034 Size: 7501 EcoRI G'AATT_C Cuts at: 4810 5188 4810 Size: 378 7123 FspI TGC'GCA Cuts at: 6007 6007 Size: 7501 HaeII r_GCGC'y Cuts at: 1678 6648 1678 Size: 4970 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3672 3704 4487 4519 4585 Size: 32 135 32 2100 32 783 32 66 Cuts at: 4585 4612 4617 4644 6142 6174 6216 6248 1373 Size: 27 5 27 1498 32 42 32 2626 Fragments arranged by size: 2626 2100 1498 783 135 66 42 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 4845 5091 669 Size: 988 281 2907 246 3079 Fragments arranged by size: 3079 2907 988 281 246 HindIII A'AGCT_T Cuts at: 748 2156 748 Size: 1408 6093 HpaI GTT'AAC Cuts at: 5091 5091 Size: 7501 KpnI G_GTAC'C Cuts at: 2697 4826 2697 Size: 2129 5372 MfeI C'AATT_G Cuts at: 5100 5100 Size: 7501 MluI A'CGCG_T Cuts at: 7377 7377 Size: 7501 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2848 3859 6615 6799 1305 Size: 1543 1011 2756 184 2007 Fragments arranged by size: 2756 2007 1543 1011 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3752 10 Size: 54 1425 343 1920 3759 Fragments arranged by size: 3759 1920 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 2193 2345 2961 5459 5818 5977 Size: 648 942 85 152 616 2498 359 159 Cuts at: 5977 518 Size: 2042 Fragments arranged by size: 2498 2042 942 648 616 359 159 152 85 MspA1I CmG'CkG Cuts at: 1606 3937 4917 5594 6742 6987 1606 Size: 2331 980 677 1148 245 2120 Fragments arranged by size: 2331 2120 1148 980 677 245 NaeI GCC'GGC Cuts at: 2017 2017 Size: 7501 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6949 NdeI CA'TA_TG Cuts at: 387 387 Size: 7501 NgoMIV G'CCGG_C Cuts at: 2015 2015 Size: 7501 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7501 NotI GC'GGCC_GC Cuts at: 4948 4948 Size: 7501 NspI r_CATG'y Cuts at: 1132 1229 2216 3842 4150 4859 6404 1132 Size: 97 987 1626 308 709 1545 2229 Fragments arranged by size: 2229 1626 1545 987 709 308 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7501 PciI A'CATG_T Cuts at: 6400 6400 Size: 7501 PflMI CCAn_nnn'nTGG Cuts at: 1108 3977 4076 1108 Size: 2869 99 4533 Fragments arranged by size: 4533 2869 99 PfoI T'CCnGG_A Cuts at: 4341 4341 Size: 7501 PmeI GTTT'AAAC Cuts at: 4805 4805 Size: 7501 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3527 3559 5570 5602 7083 7115 3527 Size: 32 2011 32 1481 32 3913 Fragments arranged by size: 3913 2011 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2034 2034 Size: 7501 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7501 PsiI TTA'TAA Cuts at: 5071 5071 Size: 7501 PstI C_TGCA'G Cuts at: 830 4853 830 Size: 4023 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7501 PvuII CAG'CTG Cuts at: 3937 3937 Size: 7501 SalI G'TCGA_C Cuts at: 1936 4843 1936 Size: 2907 4594 SanDI GG'GwC_CC Cuts at: 2034 2034 Size: 7501 SbfI CC_TGCA'GG Cuts at: 4853 4853 Size: 7501 ScaI AGT'ACT Cuts at: 2172 5749 7461 2172 Size: 3577 1712 2212 Fragments arranged by size: 3577 2212 1712 SfcI C'TryA_G Cuts at: 826 1045 2248 3252 4731 4849 5984 6665 6856 Size: 219 1203 1004 1479 118 1135 681 191 Cuts at: 6856 826 Size: 1471 Fragments arranged by size: 1479 1471 1203 1135 1004 681 219 191 118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7501 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7501 SmaI CCC'GGG Cuts at: 1872 4828 1872 Size: 2956 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5605 6506 6768 820 Size: 197 926 3662 901 262 1553 Fragments arranged by size: 3662 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5292 493 Size: 4799 2702 SpeI A'CTAG_T Cuts at: 152 2065 152 Size: 1913 5588 SphI G_CATG'C Cuts at: 1132 4859 1132 Size: 3727 3774 SspI AAT'ATT Cuts at: 5 52 2745 2830 5425 5 Size: 47 2693 85 2595 2081 Fragments arranged by size: 2693 2595 2081 85 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7501 StyI C'CwwG_G Cuts at: 513 1065 2301 4940 513 Size: 552 1236 2639 3074 Fragments arranged by size: 3074 2639 1236 552 SwaI ATTT'AAAT Cuts at: 3303 3303 Size: 7501 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2462 3984 4548 5528 5545 5698 5883 Size: 1255 882 1522 564 980 17 153 185 Cuts at: 5883 6328 325 Size: 445 1498 Fragments arranged by size: 1522 1498 1255 980 882 564 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2170 2678 3187 Size: 274 80 33 51 1443 193 508 509 Cuts at: 3187 3952 4643 5747 7459 96 Size: 765 691 1104 1712 138 Fragments arranged by size: 1712 1443 1104 765 691 509 508 274 193 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4155 4187 4213 4245 7461 7493 4155 Size: 32 26 32 3216 32 4163 Fragments arranged by size: 4163 3216 32 32 32 26 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7501 XbaI T'CTAG_A Cuts at: 4837 4837 Size: 7501 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3745 3974 3745 Size: 229 7272 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7501 XmaI C'CCGG_G Cuts at: 1870 4826 1870 Size: 2956 4545 XmnI GAAnn'nnTTC Cuts at: 2311 3379 5630 2311 Size: 1068 2251 4182 Fragments arranged by size: 4182 2251 1068 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5309 276 Size: 53 83 186 936 3775 2468 Fragments arranged by size: 3775 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI