Restriction map for
pFN21AB5599
(Linear) (Six-base) MAPSORT of: KIBB5599.seq Check: 1765 from: 1 to: 2796
KIBB5599.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1065 2242 2796
Size: 1065 1177 554
Fragments arranged by size:
1177 1065 554
Acc65I G'GTAC_C
Cuts at: 0 688 2796
Size: 688 2108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1795 1886 2796
Size: 1795 91 910
Fragments arranged by size:
1795 910 91
AleI CACnn'nnGTG
Cuts at: 0 103 340 2796
Size: 103 237 2456
Fragments arranged by size:
2456 237 103
AlwNI CAG_nnn'CTG
Cuts at: 0 658 1932 2677 2796
Size: 658 1274 745 119
Fragments arranged by size:
1274 745 658 119
ApoI r'AATT_y
Cuts at: 0 304 364 703 1213 1293 2511 2520 2796
Size: 304 60 339 510 80 1218 9 276
Fragments arranged by size:
1218 510 339 304 276 80 60 9
AseI AT'TA_AT
Cuts at: 0 614 2796
Size: 614 2182
BanI G'GyrC_C
Cuts at: 0 688 2796
Size: 688 2108
BanII G_rGCy'C
Cuts at: 0 128 1194 1643 2796
Size: 128 1066 449 1153
Fragments arranged by size:
1153 1066 449 128
BbsI GAAGACnn'nnnn_
Cuts at: 0 1285 1439 2492 2796
Size: 1285 154 1053 304
Fragments arranged by size:
1285 1053 304 154
BciVI GTATCCnnnnn_n'
Cuts at: 0 2259 2796
Size: 2259 537
BclI T'GATC_A
Cuts at: 0 180 2490 2796
Size: 180 2310 306
Fragments arranged by size:
2310 306 180
Bme1580I G_kGCm'C
Cuts at: 0 476 2796
Size: 476 2320
BmrI ACTGGGnnnn_n'
Cuts at: 0 1197 2796
Size: 1197 1599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 2198 2796
Size: 854 1344 598
Fragments arranged by size:
1344 854 598
Bpu10I CC'TnA_GC
Cuts at: 0 1757 2796
Size: 1757 1039
BsaBI GATnn'nnATC
Cuts at: 0 2387 2690 2796
Size: 2387 303 106
Fragments arranged by size:
2387 303 106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 827 857 2208 2238 2796
Size: 827 30 1351 30 558
Fragments arranged by size:
1351 827 558 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 1708 2353 2362 2796
Size: 154 1554 645 9 434
Fragments arranged by size:
1554 645 434 154 9
BseYI C'CCAG_C
Cuts at: 0 41 2705 2796
Size: 41 2664 91
Fragments arranged by size:
2664 91 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 2796
Size: 704 2092
BsiEI CG_ry'CG
Cuts at: 0 18 77 2796
Size: 18 59 2719
Fragments arranged by size:
2719 59 18
BsiHKAI G_wGCw'C
Cuts at: 0 2363 2796
Size: 2363 433
BsmI GAATG_Cn'
Cuts at: 0 1032 2178 2796
Size: 1032 1146 618
Fragments arranged by size:
1146 1032 618
Bsp1286I G_dGCh'C
Cuts at: 0 128 476 1194 1643 2363 2796
Size: 128 348 718 449 720 433
Fragments arranged by size:
720 718 449 433 348 128
BspHI T'CATG_A
Cuts at: 0 1113 1359 2796
Size: 1113 246 1437
Fragments arranged by size:
1437 1113 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 676 1065 1107 2242 2796
Size: 676 389 42 1135 554
Fragments arranged by size:
1135 676 554 389 42
BsrFI r'CCGG_y
Cuts at: 0 10 573 2796
Size: 10 563 2223
Fragments arranged by size:
2223 563 10
BsrGI T'GTAC_A
Cuts at: 0 673 1947 2638 2796
Size: 673 1274 691 158
Fragments arranged by size:
1274 691 673 158
BssSI C'ACGA_G
Cuts at: 0 1940 2796
Size: 1940 856
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2176 2796
Size: 2176 620
BstYI r'GATC_y
Cuts at: 0 1254 1595 2016 2282 2388 2796
Size: 1254 341 421 266 106 408
Fragments arranged by size:
1254 421 408 341 266 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 66 2796
Size: 66 2730
BtsI GCAGTG_nn'
Cuts at: 0 383 523 2108 2229 2796
Size: 383 140 1585 121 567
Fragments arranged by size:
1585 567 383 140 121
DraI TTT'AAA
Cuts at: 0 350 620 1298 1401 2796
Size: 350 270 678 103 1395
Fragments arranged by size:
1395 678 350 270 103
EaeI y'GGCC_r
Cuts at: 0 15 74 95 1745 2796
Size: 15 59 21 1650 1051
Fragments arranged by size:
1650 1051 59 21 15
EagI C'GGCC_G
Cuts at: 0 15 74 2796
Size: 15 59 2722
Fragments arranged by size:
2722 59 15
EarI CTCTTCn'nnn_
Cuts at: 0 995 2796
Size: 995 1801
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 1795 1886 2198 2796
Size: 854 941 91 312 598
Fragments arranged by size:
941 854 598 312 91
EcoO109I rG'GnC_Cy
Cuts at: 0 29 2796
Size: 29 2767
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1667 1699 2482 2514 2580 2607 2612 2639
Size: 1667 32 783 32 66 27 5 27
Cuts at: 2639 2796
Size: 157
Fragments arranged by size:
1667 783 157 66 32 32 27 27
5
HindIII A'AGCT_T
Cuts at: 0 151 2796
Size: 151 2645
KpnI G_GTAC'C
Cuts at: 0 692 2796
Size: 692 2104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 843 1854 2796
Size: 843 1011 942
Fragments arranged by size:
1011 942 843
MscI TGG'CCA
Cuts at: 0 1747 2796
Size: 1747 1049
MslI CAynn'nnrTG
Cuts at: 0 103 188 340 956 2796
Size: 103 85 152 616 1840
Fragments arranged by size:
1840 616 152 103 85
MspA1I CmG'CkG
Cuts at: 0 1932 2796
Size: 1932 864
NaeI GCC'GGC
Cuts at: 0 12 2796
Size: 12 2784
NgoMIV G'CCGG_C
Cuts at: 0 10 2796
Size: 10 2786
NspI r_CATG'y
Cuts at: 0 211 1837 2145 2796
Size: 211 1626 308 651
Fragments arranged by size:
1626 651 308 211
PflMI CCAn_nnn'nTGG
Cuts at: 0 1972 2071 2796
Size: 1972 99 725
Fragments arranged by size:
1972 725 99
PfoI T'CCnGG_A
Cuts at: 0 2336 2796
Size: 2336 460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1522 1554 2796
Size: 1522 32 1242
Fragments arranged by size:
1522 1242 32
PpuMI rG'GwC_Cy
Cuts at: 0 29 2796
Size: 29 2767
PvuII CAG'CTG
Cuts at: 0 1932 2796
Size: 1932 864
SanDI GG'GwC_CC
Cuts at: 0 29 2796
Size: 29 2767
ScaI AGT'ACT
Cuts at: 0 167 2796
Size: 167 2629
SfcI C'TryA_G
Cuts at: 0 243 1247 2726 2796
Size: 243 1004 1479 70
Fragments arranged by size:
1479 1004 243 70
SpeI A'CTAG_T
Cuts at: 0 60 2796
Size: 60 2736
SspI AAT'ATT
Cuts at: 0 740 825 2796
Size: 740 85 1971
Fragments arranged by size:
1971 740 85
StyI C'CwwG_G
Cuts at: 0 296 2796
Size: 296 2500
SwaI ATTT'AAAT
Cuts at: 0 1298 2796
Size: 1298 1498
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 457 1979 2543 2796
Size: 457 1522 564 253
Fragments arranged by size:
1522 564 457 253
TatI w'GTAC_w
Cuts at: 0 165 673 1182 1947 2638 2796
Size: 165 508 509 765 691 158
Fragments arranged by size:
765 691 509 508 165 158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2150 2182 2208 2240 2796
Size: 2150 32 26 32 556
Fragments arranged by size:
2150 556 32 32 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1740 1969 2796
Size: 1740 229 827
Fragments arranged by size:
1740 827 229
XmnI GAAnn'nnTTC
Cuts at: 0 306 1374 2796
Size: 306 1068 1422
Fragments arranged by size:
1422 1068 306
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI ApoI AseI BanI
BanII BbsI BciVI BclI Bme1580I BmrI BpmI Bpu10I
BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrFI BsrGI BssSI BstXI BstYI
BtgZI BtsI DraI EaeI EagI EarI Eco57MI EcoO109I
Hin4I HindIII KpnI MmeI MscI MslI MspA1I NaeI
NgoMIV NspI PflMI PfoI PpiI PpuMI PvuII SanDI
ScaI SfcI SpeI SspI StyI SwaI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaHI
BsaWI BsiWI BsmBI BspEI BsrBI BsrDI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HpaI KasI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI StuI Tth111I XbaI XhoI XmaI
ZraI