(Circular) (Six-base) MAPSORT of: pFN21AB5571.seq Check: 3767 from: 1 to: 7459 pFN21AB5571 7459 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5269 278 Size: 53 83 186 936 3733 2468 Fragments arranged by size: 3733 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4349 4802 1937 Size: 2412 453 4594 Fragments arranged by size: 4594 2412 453 Acc65I G'GTAC_C Cuts at: 3428 4780 3428 Size: 1352 6107 AclI AA'CG_TT Cuts at: 1563 5586 5959 1563 Size: 4023 373 3063 Fragments arranged by size: 4023 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2847 4188 5522 6906 7174 1632 Size: 1215 1341 1334 1384 268 1917 Fragments arranged by size: 1917 1384 1341 1334 1215 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7262 AflIII A'CryG_T Cuts at: 1203 6358 7335 1203 Size: 5155 977 1327 Fragments arranged by size: 5155 1327 977 AgeI A'CCGG_T Cuts at: 6290 6290 Size: 7459 AhdI GACnn_n'nnGTC Cuts at: 6188 6188 Size: 7459 AleI CACnn'nnGTG Cuts at: 1166 4179 1166 Size: 3013 4446 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7459 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3561 3593 3561 Size: 32 7427 AlwNI CAG_nnn'CTG Cuts at: 4646 5202 6774 4646 Size: 556 1572 5331 Fragments arranged by size: 5331 1572 556 ApaLI G'TGCA_C Cuts at: 5516 6672 5516 Size: 1156 6303 ApoI r'AATT_y Cuts at: 1493 2395 3818 4768 4994 5146 7255 1493 Size: 902 1423 950 226 152 2109 1697 Fragments arranged by size: 2109 1697 1423 950 902 226 152 AseI AT'TA_AT Cuts at: 160 1032 4161 6013 160 Size: 872 3129 1852 1606 Fragments arranged by size: 3129 1852 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7459 AvaI C'yCGr_G Cuts at: 1870 1943 4784 1870 Size: 73 2841 4545 Fragments arranged by size: 4545 2841 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2920 2953 2920 Size: 33 7426 BamHI G'GATC_C Cuts at: 4789 5156 4789 Size: 367 7092 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3428 4780 6235 618 Size: 325 217 627 1641 1352 1455 1842 Fragments arranged by size: 1842 1641 1455 1352 627 325 217 BanII G_rGCy'C Cuts at: 1391 4778 1391 Size: 3387 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2835 3065 4188 4324 928 Size: 971 936 230 1123 136 4063 Fragments arranged by size: 4063 1123 971 936 230 136 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5650 5684 1903 Size: 34 3713 34 3678 Fragments arranged by size: 3713 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2921 5351 6561 2921 Size: 2430 1210 3819 Fragments arranged by size: 3819 2430 1210 BclI T'GATC_A Cuts at: 1548 2694 1548 Size: 1146 6313 BfrBI ATG'CAT Cuts at: 4203 4203 Size: 7459 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6070 136 Size: 107 122 71 631 605 4398 1525 Fragments arranged by size: 4398 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4123 4387 7454 4123 Size: 264 3067 4128 Fragments arranged by size: 4128 3067 264 BlpI GC'TnA_GC Cuts at: 3883 4876 3883 Size: 993 6466 Bme1580I G_kGCm'C Cuts at: 1790 5520 6676 1790 Size: 3730 1156 2573 Fragments arranged by size: 3730 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7459 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3991 5190 6148 Size: 416 53 83 399 24 2574 1199 958 Cuts at: 6148 442 Size: 1753 Fragments arranged by size: 2574 1753 1199 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7459 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2094 2364 2953 2962 3480 6119 1239 Size: 855 270 589 9 518 2639 2579 Fragments arranged by size: 2639 2579 855 589 518 270 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3012 3389 3831 4321 5584 6449 6747 3012 Size: 377 442 490 1263 865 298 3724 Fragments arranged by size: 3724 1263 865 490 442 377 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3930 6122 882 Size: 617 2431 2192 2219 Fragments arranged by size: 2431 2219 2192 617 BsaAI yAC'GTr Cuts at: 493 1204 5250 493 Size: 711 4046 2702 Fragments arranged by size: 4046 2702 711 BsaBI GATnn'nnATC Cuts at: 3124 3189 7453 3124 Size: 65 4264 3130 Fragments arranged by size: 4264 3130 65 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5266 5648 6283 275 Size: 53 83 186 936 3733 382 635 1451 Fragments arranged by size: 3733 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5892 6290 6564 6711 7122 5892 Size: 398 274 147 411 6229 Fragments arranged by size: 6229 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3052 3082 3478 3508 1178 Size: 30 165 30 1649 30 396 30 5129 Fragments arranged by size: 5129 1649 396 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4005 4388 1186 Size: 2819 383 4257 Fragments arranged by size: 4257 2819 383 BseYI C'CCAG_C Cuts at: 4353 6662 7158 4353 Size: 2309 496 4654 Fragments arranged by size: 4654 2309 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6886 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4909 5670 6698 1540 Size: 275 186 2908 761 1028 2301 Fragments arranged by size: 2908 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4271 5520 5605 6676 4271 Size: 1249 85 1071 5054 Fragments arranged by size: 5054 1249 1071 85 BsmI GAATG_Cn' Cuts at: 2614 3299 3887 4970 5063 2614 Size: 685 588 1083 93 5010 Fragments arranged by size: 5010 1083 685 588 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4271 4778 5520 5605 6676 1391 Size: 399 2481 507 742 85 1071 2174 Fragments arranged by size: 2481 2174 1071 742 507 399 85 BspEI T'CCGG_A Cuts at: 7122 7122 Size: 7459 BspHI T'CATG_A Cuts at: 5348 7078 5348 Size: 1730 5729 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4814 844 Size: 3970 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4378 5346 1394 Size: 429 2555 968 3507 Fragments arranged by size: 3507 2555 968 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3062 5954 6128 65 Size: 1183 1814 2892 174 1396 Fragments arranged by size: 2892 1814 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6103 6290 1700 Size: 110 4293 187 2869 Fragments arranged by size: 4293 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2116 3043 3765 96 Size: 2020 927 722 3790 Fragments arranged by size: 3790 2020 927 722 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7459 BssSI C'ACGA_G Cuts at: 3050 5519 6531 3050 Size: 2469 1012 3978 Fragments arranged by size: 3978 2469 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2609 4628 794 Size: 1815 2019 3625 Fragments arranged by size: 3625 2019 1815 BstBI TT'CG_AA Cuts at: 6263 6263 Size: 7459 BstEII G'GTnAC_C Cuts at: 1184 4002 1184 Size: 2818 4641 BstXI CCAn_nnnn'nTGG Cuts at: 3000 3000 Size: 7459 BstYI r'GATC_y Cuts at: 1972 2080 3013 3289 3973 4123 4340 4387 4586 Size: 108 933 276 684 150 217 47 199 Cuts at: 4586 4789 5156 5541 5558 7010 7096 7108 7454 Size: 203 367 385 17 1452 86 12 346 Cuts at: 7454 1972 Size: 1977 Fragments arranged by size: 1977 1452 933 684 385 367 346 276 217 203 199 150 108 86 47 17 12 Bsu36I CC'TnA_GG Cuts at: 2229 4755 2229 Size: 2526 4933 BtgI C'CryG_G Cuts at: 513 1065 4287 513 Size: 552 3222 3685 Fragments arranged by size: 3685 3222 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3422 487 Size: 683 56 2196 4524 Fragments arranged by size: 4524 2196 683 56 BtsI GCAGTG_nn' Cuts at: 2136 4186 4240 4975 5769 5789 2136 Size: 2050 54 735 794 20 3806 Fragments arranged by size: 3806 2050 794 735 54 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7459 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3035 3070 529 Size: 35 2471 35 4918 Fragments arranged by size: 4918 2471 35 35 DraI TTT'AAA Cuts at: 2484 4763 2484 Size: 2279 5180 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6466 809 Size: 507 5150 1802 Fragments arranged by size: 5150 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4379 4906 5795 Size: 54 1425 50 275 18 2549 527 889 Cuts at: 5795 7424 8 Size: 1629 43 Fragments arranged by size: 2549 1629 1425 889 527 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4906 1537 Size: 275 3094 4090 Fragments arranged by size: 4090 3094 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3909 5389 1353 Size: 2556 1480 3423 Fragments arranged by size: 3423 2556 1480 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2664 6046 6420 6566 387 Size: 1064 138 1075 3382 374 146 1280 Fragments arranged by size: 3382 1280 1075 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2364 2847 2953 2962 3480 4188 Size: 393 462 270 483 106 9 518 708 Cuts at: 4188 5522 6119 6906 7174 1239 Size: 1334 597 787 268 1524 Fragments arranged by size: 1524 1334 787 708 597 518 483 462 393 270 268 106 9 EcoNI CCTnn'n_nnAGG Cuts at: 2618 3564 2618 Size: 946 6513 EcoO109I rG'GnC_Cy Cuts at: 3569 3569 Size: 7459 EcoRI G'AATT_C Cuts at: 3818 4768 5146 3818 Size: 950 378 6131 Fragments arranged by size: 6131 950 378 FspI TGC'GCA Cuts at: 5965 5965 Size: 7459 HaeII r_GCGC'y Cuts at: 1678 6606 1678 Size: 4928 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2696 2728 2822 2854 2966 Size: 32 135 32 1124 32 94 32 112 Cuts at: 2966 2998 3052 3084 3157 3189 4115 4147 4549 Size: 32 54 32 73 32 926 32 402 Cuts at: 4549 4581 6100 6132 6174 6206 1373 Size: 32 1519 32 42 32 2626 Fragments arranged by size: 2626 1519 1124 926 402 135 112 94 73 54 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2010 2581 4803 5049 669 Size: 988 281 72 571 2222 246 3079 Fragments arranged by size: 3079 2222 988 571 281 246 72 HindIII A'AGCT_T Cuts at: 748 748 Size: 7459 HpaI GTT'AAC Cuts at: 5049 5049 Size: 7459 KpnI G_GTAC'C Cuts at: 3432 4784 3432 Size: 1352 6107 MfeI C'AATT_G Cuts at: 5058 5058 Size: 7459 MluI A'CGCG_T Cuts at: 7335 7335 Size: 7459 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6573 6757 1305 Size: 5268 184 2007 Fragments arranged by size: 5268 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5637 Fragments arranged by size: 5637 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2184 3869 4179 4410 5417 5776 5935 Size: 648 1018 1685 310 231 1007 359 159 Cuts at: 5935 518 Size: 2042 Fragments arranged by size: 2042 1685 1018 1007 648 359 310 231 159 MspA1I CmG'CkG Cuts at: 1606 3239 4875 5552 6700 6945 1606 Size: 1633 1636 677 1148 245 2120 Fragments arranged by size: 2120 1636 1633 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6907 NdeI CA'TA_TG Cuts at: 387 387 Size: 7459 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7459 NotI GC'GGCC_GC Cuts at: 4906 4906 Size: 7459 NsiI A_TGCA'T Cuts at: 4205 4205 Size: 7459 NspI r_CATG'y Cuts at: 1132 1229 3319 4207 4708 4753 4817 6362 1132 Size: 97 2090 888 501 45 64 1545 2229 Fragments arranged by size: 2229 2090 1545 888 501 97 64 45 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7459 PciI A'CATG_T Cuts at: 6358 6358 Size: 7459 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7459 PmeI GTTT'AAAC Cuts at: 4763 4763 Size: 7459 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5528 5560 7041 7073 5528 Size: 32 1481 32 5914 Fragments arranged by size: 5914 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3569 3569 Size: 7459 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7459 PsiI TTA'TAA Cuts at: 2762 5029 2762 Size: 2267 5192 PstI C_TGCA'G Cuts at: 830 2143 4463 4624 4811 830 Size: 1313 2320 161 187 3478 Fragments arranged by size: 3478 2320 1313 187 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7459 PvuII CAG'CTG Cuts at: 3239 3239 Size: 7459 SalI G'TCGA_C Cuts at: 1936 4801 1936 Size: 2865 4594 SbfI CC_TGCA'GG Cuts at: 4811 4811 Size: 7459 ScaI AGT'ACT Cuts at: 4445 5707 7419 4445 Size: 1262 1712 4485 Fragments arranged by size: 4485 1712 1262 SfcI C'TryA_G Cuts at: 826 1045 2064 2139 3034 4303 4459 4620 4807 Size: 219 1019 75 895 1269 156 161 187 Cuts at: 4807 5942 6623 6814 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1269 1135 1019 895 681 219 191 187 161 156 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7459 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7459 SmaI CCC'GGG Cuts at: 1872 4786 1872 Size: 2914 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3027 3368 3846 4336 5563 6464 Size: 197 926 1084 341 478 490 1227 901 Cuts at: 6464 6726 820 Size: 262 1553 Fragments arranged by size: 1553 1227 1084 926 901 490 478 341 262 197 SnaBI TAC'GTA Cuts at: 493 5250 493 Size: 4757 2702 SpeI A'CTAG_T Cuts at: 152 3752 152 Size: 3600 3859 SphI G_CATG'C Cuts at: 1132 4708 4817 1132 Size: 3576 109 3774 Fragments arranged by size: 3774 3576 109 SspI AAT'ATT Cuts at: 5 52 2299 5383 5 Size: 47 2247 3084 2081 Fragments arranged by size: 3084 2247 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7459 StyI C'CwwG_G Cuts at: 513 1065 2614 3341 4019 4898 513 Size: 552 1549 727 678 879 3074 Fragments arranged by size: 3074 1549 879 727 678 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3313 5486 5503 5656 5841 6286 325 Size: 1255 1733 2173 17 153 185 445 1498 Fragments arranged by size: 2173 1733 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2116 3043 3765 Size: 274 80 33 51 1443 139 927 722 Cuts at: 3765 4443 5705 7417 96 Size: 678 1262 1712 138 Fragments arranged by size: 1712 1443 1262 927 722 678 274 139 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7419 7451 7419 Size: 32 7427 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7459 XbaI T'CTAG_A Cuts at: 2077 4795 2077 Size: 2718 4741 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7459 XmaI C'CCGG_G Cuts at: 1870 4784 1870 Size: 2914 4545 XmnI GAAnn'nnTTC Cuts at: 5588 5588 Size: 7459 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5267 276 Size: 53 83 186 936 3733 2468 Fragments arranged by size: 3733 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI