(Circular) (Six-base) MAPSORT of: pFN21AB5541.seq Check: 1205 from: 1 to: 7636 pFN21AB5541 7636 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2652 3024 4413 5446 Size: 53 83 186 936 1116 372 1389 1033 Cuts at: 5446 278 Size: 2468 Fragments arranged by size: 2468 1389 1116 1033 936 372 186 83 53 AccI GT'mk_AC Cuts at: 1937 2252 3119 4979 1937 Size: 315 867 1860 4594 Fragments arranged by size: 4594 1860 867 315 Acc65I G'GTAC_C Cuts at: 4957 4957 Size: 7636 AclI AA'CG_TT Cuts at: 1563 5763 6136 1563 Size: 4200 373 3063 Fragments arranged by size: 4200 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3219 5699 7083 7351 1632 Size: 1587 2480 1384 268 1917 Fragments arranged by size: 2480 1917 1587 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3326 820 Size: 197 2309 5130 Fragments arranged by size: 5130 2309 197 AflIII A'CryG_T Cuts at: 1203 2203 3540 6535 7512 1203 Size: 1000 1337 2995 977 1327 Fragments arranged by size: 2995 1337 1327 1000 977 AgeI A'CCGG_T Cuts at: 6467 6467 Size: 7636 AhdI GACnn_n'nnGTC Cuts at: 6365 6365 Size: 7636 AleI CACnn'nnGTG Cuts at: 1166 3758 1166 Size: 2592 5044 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2344 3377 4355 1184 Size: 1160 1033 978 4465 Fragments arranged by size: 4465 1160 1033 978 AlwNI CAG_nnn'CTG Cuts at: 3469 3490 5379 6951 3469 Size: 21 1889 1572 4154 Fragments arranged by size: 4154 1889 1572 21 ApaI G_GGCC'C Cuts at: 2961 2961 Size: 7636 ApaLI G'TGCA_C Cuts at: 5693 6849 5693 Size: 1156 6480 ApoI r'AATT_y Cuts at: 1493 3708 4667 4945 5171 5323 7432 1493 Size: 2215 959 278 226 152 2109 1697 Fragments arranged by size: 2215 2109 1697 959 278 226 152 AseI AT'TA_AT Cuts at: 160 1032 6190 160 Size: 872 5158 1606 Fragments arranged by size: 5158 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7636 AvaI C'yCGr_G Cuts at: 1870 1943 2054 2514 3261 3302 3565 3812 4024 Size: 73 111 460 747 41 263 247 212 Cuts at: 4024 4961 1870 Size: 937 4545 Fragments arranged by size: 4545 937 747 460 263 247 212 111 73 41 BamHI G'GATC_C Cuts at: 2658 3804 4450 4966 5333 2658 Size: 1146 646 516 367 4961 Fragments arranged by size: 4961 1146 646 516 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3569 4195 4367 4957 6412 Size: 325 217 627 1782 626 172 590 1455 Cuts at: 6412 618 Size: 1842 Fragments arranged by size: 1842 1782 1455 627 626 590 325 217 172 BanII G_rGCy'C Cuts at: 1391 2523 2961 3534 3566 3999 4899 4908 4955 Size: 1132 438 573 32 433 900 9 47 Cuts at: 4955 1391 Size: 4072 Fragments arranged by size: 4072 1132 900 573 438 433 47 32 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4245 4413 928 Size: 971 2346 168 4151 Fragments arranged by size: 4151 2346 971 168 BbvCI CC'TCA_GC Cuts at: 3747 4468 3747 Size: 721 6915 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2897 2931 3920 3954 4462 Size: 34 50 34 876 34 989 34 508 Cuts at: 4462 4496 5827 5861 1903 Size: 34 1331 34 3678 Fragments arranged by size: 3678 1331 989 876 508 50 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5528 6738 5528 Size: 1210 6426 BclI T'GATC_A Cuts at: 1548 3003 1548 Size: 1455 6181 BfrBI ATG'CAT Cuts at: 3409 3409 Size: 7636 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2014 3638 Size: 107 122 71 631 605 337 5 1624 Cuts at: 3638 6247 136 Size: 2609 1525 Fragments arranged by size: 2609 1624 1525 631 605 337 122 107 71 5 BglII A'GATC_T Cuts at: 4699 7631 4699 Size: 2932 4704 BlpI GC'TnA_GC Cuts at: 4232 5053 4232 Size: 821 6815 Bme1580I G_kGCm'C Cuts at: 1790 2928 2961 3572 3755 5697 6853 1790 Size: 1138 33 611 183 1942 1156 2573 Fragments arranged by size: 2573 1942 1156 1138 611 183 33 BmgBI CAC'GTC Cuts at: 1318 2575 3543 3588 3790 4537 4806 1318 Size: 1257 968 45 202 747 269 4148 Fragments arranged by size: 4148 1257 968 747 269 202 45 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4910 5367 6325 Size: 416 53 83 399 24 3493 457 958 Cuts at: 6325 442 Size: 1753 Fragments arranged by size: 3493 1753 958 457 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7636 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4721 4721 Size: 7636 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3152 3410 3473 3528 4013 4206 4505 4522 Size: 1913 258 63 55 485 193 299 17 Cuts at: 4522 4634 4908 6296 1239 Size: 112 274 1388 2579 Fragments arranged by size: 2579 1913 1388 485 299 274 258 193 112 63 55 17 Bpu10I CC'TnA_GC Cuts at: 3350 3401 3519 3747 4468 3350 Size: 51 118 228 721 6518 Fragments arranged by size: 6518 721 228 118 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3088 5761 6626 6924 3088 Size: 2673 865 298 3800 Fragments arranged by size: 3800 2673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2741 4059 6299 882 Size: 617 1242 1318 2240 2219 Fragments arranged by size: 2240 2219 1318 1242 617 BsaAI yAC'GTr Cuts at: 493 1204 4303 4421 4480 5427 493 Size: 711 3099 118 59 947 2702 Fragments arranged by size: 3099 2702 947 711 118 59 BsaBI GATnn'nnATC Cuts at: 2729 7630 2729 Size: 4901 2735 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2649 2846 2973 3021 Size: 53 83 186 936 1116 197 127 48 Cuts at: 3021 4410 4929 5443 5825 6460 275 Size: 1389 519 514 382 635 1451 Fragments arranged by size: 1451 1389 1116 936 635 519 514 382 197 186 127 83 53 48 BsaWI w'CCGG_w Cuts at: 2399 2523 6069 6467 6741 6888 7299 2399 Size: 124 3546 398 274 147 411 2736 Fragments arranged by size: 3546 2736 411 398 274 147 124 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2657 2687 4028 4058 1178 Size: 30 165 30 1254 30 1341 30 4756 Fragments arranged by size: 4756 1341 1254 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3287 4694 4814 1186 Size: 2101 1407 120 4008 Fragments arranged by size: 4008 2101 1407 120 BseYI C'CCAG_C Cuts at: 3011 3372 3581 4768 4908 6839 7335 3011 Size: 361 209 1187 140 1931 496 3312 Fragments arranged by size: 3312 1931 1187 496 361 209 140 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2336 3877 3960 4876 1163 Size: 573 600 1541 83 916 3923 Fragments arranged by size: 3923 1541 916 600 573 83 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4578 5086 5847 6875 1540 Size: 275 186 2577 508 761 1028 2301 Fragments arranged by size: 2577 2301 1028 761 508 275 186 BsiHKAI G_wGCw'C Cuts at: 2523 3356 3534 3566 3870 4485 5697 5782 6853 Size: 833 178 32 304 615 1212 85 1071 Cuts at: 6853 2523 Size: 3306 Fragments arranged by size: 3306 1212 1071 833 615 304 178 85 32 BsmI GAATG_Cn' Cuts at: 2536 5147 5240 2536 Size: 2611 93 4932 Fragments arranged by size: 4932 2611 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4757 4812 4757 Size: 55 7581 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2523 2928 2961 3356 3534 3566 3572 Size: 399 733 405 33 395 178 32 6 Cuts at: 3572 3755 3870 3999 4485 4899 4908 4955 5697 Size: 183 115 129 486 414 9 47 742 Cuts at: 5697 5782 6853 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 733 486 414 405 399 395 183 178 129 115 85 47 33 32 9 6 BspEI T'CCGG_A Cuts at: 7299 7299 Size: 7636 BspHI T'CATG_A Cuts at: 5525 7255 5525 Size: 1730 5906 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2182 4668 4991 844 Size: 1338 2486 323 3489 Fragments arranged by size: 3489 2486 1338 323 BsrBI CCG'CTC Cuts at: 1394 1823 5523 1394 Size: 429 3700 3507 Fragments arranged by size: 3700 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3921 4413 6131 6305 65 Size: 1183 2673 492 1718 174 1396 Fragments arranged by size: 2673 1718 1396 1183 492 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 3906 6280 6467 1700 Size: 110 198 1898 2374 187 2869 Fragments arranged by size: 2869 2374 1898 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7636 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7636 BssSI C'ACGA_G Cuts at: 2906 4862 4892 5696 6708 2906 Size: 1956 30 804 1012 3834 Fragments arranged by size: 3834 1956 1012 804 30 BstAPI GCAn_nnn'nTGC Cuts at: 794 3490 794 Size: 2696 4940 BstBI TT'CG_AA Cuts at: 6440 6440 Size: 7636 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7636 BstXI CCAn_nnnn'nTGG Cuts at: 2969 3130 3506 4328 2969 Size: 161 376 822 6277 Fragments arranged by size: 6277 822 376 161 BstYI r'GATC_y Cuts at: 1972 2179 2230 2658 3459 3804 4450 4699 4966 Size: 207 51 428 801 345 646 249 267 Cuts at: 4966 5333 5718 5735 7187 7273 7285 7631 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 801 646 428 385 367 346 345 267 249 207 86 51 17 12 BstZ17I GTA'TAC Cuts at: 2253 2253 Size: 7636 Bsu36I CC'TnA_GG Cuts at: 2565 2565 Size: 7636 BtgI C'CryG_G Cuts at: 513 1065 2929 3312 3739 4608 4845 513 Size: 552 1864 383 427 869 237 3304 Fragments arranged by size: 3304 1864 869 552 427 383 237 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4460 487 Size: 683 56 3234 3663 Fragments arranged by size: 3663 3234 683 56 BtsI GCAGTG_nn' Cuts at: 2558 4167 5152 5946 5966 2558 Size: 1609 985 794 20 4228 Fragments arranged by size: 4228 1609 985 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7636 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2187 2222 2524 2559 529 Size: 35 1623 35 302 35 5606 Fragments arranged by size: 5606 1623 302 35 35 35 DraI TTT'AAA Cuts at: 2172 4940 2172 Size: 2768 4868 DraIII CAC_nnn'GTG Cuts at: 2599 2799 2599 Size: 200 7436 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2770 3839 4771 6643 809 Size: 507 1454 1069 932 1872 1802 Fragments arranged by size: 1872 1802 1454 1069 932 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3792 5083 5972 Size: 54 1425 50 275 18 1962 1291 889 Cuts at: 5972 7601 8 Size: 1629 43 Fragments arranged by size: 1962 1629 1425 1291 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5083 1537 Size: 275 3271 4090 Fragments arranged by size: 4090 3271 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2205 2466 3794 4877 5566 1353 Size: 852 261 1328 1083 689 3423 Fragments arranged by size: 3423 1328 1083 852 689 261 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6223 6597 6743 387 Size: 1064 138 4634 374 146 1280 Fragments arranged by size: 4634 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2521 3532 3564 2521 Size: 1011 32 6593 Fragments arranged by size: 6593 1011 32 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3152 3219 3410 3473 3528 4013 4206 Size: 393 1520 67 191 63 55 485 193 Cuts at: 4206 4505 4522 4634 4908 5699 6296 7083 7351 Size: 299 17 112 274 791 597 787 268 Cuts at: 7351 1239 Size: 1524 Fragments arranged by size: 1524 1520 791 787 597 485 393 299 274 268 193 191 112 67 63 55 17 EcoO109I rG'GnC_Cy Cuts at: 2194 2957 2958 3307 3597 4674 4773 2194 Size: 763 1 349 290 1077 99 5057 Fragments arranged by size: 5057 1077 763 349 290 99 1 EcoRI G'AATT_C Cuts at: 4945 5323 4945 Size: 378 7258 FspI TGC'GCA Cuts at: 3981 6142 3981 Size: 2161 5475 HaeII r_GCGC'y Cuts at: 1678 6783 1678 Size: 5105 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2624 2656 2836 2868 3394 Size: 32 135 32 1052 32 180 32 526 Cuts at: 3394 3426 4438 4470 6277 6309 6351 6383 1373 Size: 32 1012 32 1807 32 42 32 2626 Fragments arranged by size: 2626 1807 1052 1012 526 180 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2112 3226 4980 5226 669 Size: 988 281 174 1114 1754 246 3079 Fragments arranged by size: 3079 1754 1114 988 281 246 174 HindIII A'AGCT_T Cuts at: 748 748 Size: 7636 HpaI GTT'AAC Cuts at: 5226 5226 Size: 7636 KpnI G_GTAC'C Cuts at: 4961 4961 Size: 7636 MfeI C'AATT_G Cuts at: 3756 5235 3756 Size: 1479 6157 MluI A'CGCG_T Cuts at: 7512 7512 Size: 7636 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3627 4158 6750 6934 1305 Size: 2322 531 2592 184 2007 Fragments arranged by size: 2592 2322 2007 531 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5814 Fragments arranged by size: 5814 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2326 2495 2733 3758 4094 5594 5953 Size: 648 1160 169 238 1025 336 1500 359 Cuts at: 5953 6112 518 Size: 159 2042 Fragments arranged by size: 2042 1500 1160 1025 648 359 336 238 169 159 MspA1I CmG'CkG Cuts at: 1606 2704 2931 3490 3847 4345 5052 5729 6877 Size: 1098 227 559 357 498 707 677 1148 Cuts at: 6877 7122 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1098 707 677 559 498 357 245 227 NaeI GCC'GGC Cuts at: 2010 2010 Size: 7636 NcoI C'CATG_G Cuts at: 513 1065 3739 4845 513 Size: 552 2674 1106 3304 Fragments arranged by size: 3304 2674 1106 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7636 NgoMIV G'CCGG_C Cuts at: 2008 2008 Size: 7636 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7636 NotI GC'GGCC_GC Cuts at: 5083 5083 Size: 7636 NsiI A_TGCA'T Cuts at: 3411 3411 Size: 7636 NspI r_CATG'y Cuts at: 1132 1229 2135 2207 3409 3775 4994 6539 1132 Size: 97 906 72 1202 366 1219 1545 2229 Fragments arranged by size: 2229 1545 1219 1202 906 366 97 72 PasI CC'CwG_GG Cuts at: 1348 3594 1348 Size: 2246 5390 PciI A'CATG_T Cuts at: 2203 6535 2203 Size: 4332 3304 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7636 PfoI T'CCnGG_A Cuts at: 3034 3534 4530 3034 Size: 500 996 6140 Fragments arranged by size: 6140 996 500 PmeI GTTT'AAAC Cuts at: 4940 4940 Size: 7636 PmlI CAC'GTG Cuts at: 4421 4480 4421 Size: 59 7577 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5705 5737 7218 7250 5705 Size: 32 1481 32 6091 Fragments arranged by size: 6091 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2194 4674 4773 2194 Size: 2480 99 5057 Fragments arranged by size: 5057 2480 99 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7636 PsiI TTA'TAA Cuts at: 5206 5206 Size: 7636 PspOMI G'GGCC_C Cuts at: 2957 2957 Size: 7636 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4244 4276 4244 Size: 32 7604 PstI C_TGCA'G Cuts at: 830 2193 3474 4014 4285 4344 4376 4753 4988 Size: 1363 1281 540 271 59 32 377 235 Cuts at: 4988 830 Size: 3478 Fragments arranged by size: 3478 1363 1281 540 377 271 235 59 32 PvuI CG_AT'CG Cuts at: 2001 4578 2001 Size: 2577 5059 PvuII CAG'CTG Cuts at: 3490 3847 3490 Size: 357 7279 SacI G_AGCT'C Cuts at: 2523 3534 3566 2523 Size: 1011 32 6593 Fragments arranged by size: 6593 1011 32 SacII CC_GC'GG Cuts at: 2932 2932 Size: 7636 SalI G'TCGA_C Cuts at: 1936 4978 1936 Size: 3042 4594 SanDI GG'GwC_CC Cuts at: 4773 4773 Size: 7636 SapI GCTCTTCn'nnn_ Cuts at: 2205 2205 Size: 7636 SbfI CC_TGCA'GG Cuts at: 2193 4988 2193 Size: 2795 4841 ScaI AGT'ACT Cuts at: 5884 7596 5884 Size: 1712 5924 SexAI A'CCwGG_T Cuts at: 3219 3219 Size: 7636 SfcI C'TryA_G Cuts at: 826 1045 2189 3470 4010 4281 4340 4372 4749 Size: 219 1144 1281 540 271 59 32 377 Cuts at: 4749 4984 6119 6800 6991 826 Size: 235 1135 681 191 1471 Fragments arranged by size: 1471 1281 1144 1135 681 540 377 271 235 219 191 59 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7636 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7636 SmaI CCC'GGG Cuts at: 1872 4963 1872 Size: 3091 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3067 3326 5740 6641 6903 820 Size: 197 926 1124 259 2414 901 262 1553 Fragments arranged by size: 2414 1553 1124 926 901 262 259 197 SnaBI TAC'GTA Cuts at: 493 4303 5427 493 Size: 3810 1124 2702 Fragments arranged by size: 3810 2702 1124 SpeI A'CTAG_T Cuts at: 152 152 Size: 7636 SphI G_CATG'C Cuts at: 1132 2135 3409 4994 1132 Size: 1003 1274 1585 3774 Fragments arranged by size: 3774 1585 1274 1003 SspI AAT'ATT Cuts at: 5 52 5560 5 Size: 47 5508 2081 Fragments arranged by size: 5508 2081 47 StuI AGG'CCT Cuts at: 1517 2280 1517 Size: 763 6873 StyI C'CwwG_G Cuts at: 513 1065 2817 3636 3723 3739 4845 5075 513 Size: 552 1752 819 87 16 1106 230 3074 Fragments arranged by size: 3074 1752 1106 819 552 230 87 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3364 3516 4355 4407 5663 5680 5833 Size: 1255 1784 152 839 52 1256 17 153 Cuts at: 5833 6018 6463 325 Size: 185 445 1498 Fragments arranged by size: 1784 1498 1256 1255 839 445 185 153 152 52 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3918 5882 7594 Size: 274 80 33 51 1443 1941 1964 1712 Cuts at: 7594 96 Size: 138 Fragments arranged by size: 1964 1941 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2585 2617 2639 2671 4028 4060 4068 4100 7596 Size: 32 22 32 1357 32 8 32 3496 Cuts at: 7596 7628 2585 Size: 32 2593 Fragments arranged by size: 3496 2593 1357 32 32 32 32 32 22 8 Tth111I GACn'n_nGTC Cuts at: 1316 2419 1316 Size: 1103 6533 XbaI T'CTAG_A Cuts at: 4972 4972 Size: 7636 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2308 3730 4027 4360 2308 Size: 1422 297 333 5584 Fragments arranged by size: 5584 1422 333 297 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7636 XmaI C'CCGG_G Cuts at: 1870 4961 1870 Size: 3091 4545 XmnI GAAnn'nnTTC Cuts at: 5765 5765 Size: 7636 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2650 3022 4411 5444 Size: 53 83 186 936 1116 372 1389 1033 Cuts at: 5444 276 Size: 2468 Fragments arranged by size: 2468 1389 1116 1033 936 372 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PspXI RsrII SfiI SfoI SrfI SwaI