(Circular) (Six-base) MAPSORT of: pFN21AB5522.seq Check: 7037 from: 1 to: 5851 pFN21AB5522 5851 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3661 278 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3194 1937 Size: 1257 4594 Acc65I G'GTAC_C Cuts at: 3172 3172 Size: 5851 AclI AA'CG_TT Cuts at: 1563 3978 4351 1563 Size: 2415 373 3063 Fragments arranged by size: 3063 2415 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2860 3914 5298 5566 1632 Size: 1228 1054 1384 268 1917 Fragments arranged by size: 1917 1384 1228 1054 268 AfeI AGC'GCT Cuts at: 2429 2429 Size: 5851 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5654 AflIII A'CryG_T Cuts at: 1203 4750 5727 1203 Size: 3547 977 1327 Fragments arranged by size: 3547 1327 977 AgeI A'CCGG_T Cuts at: 4682 4682 Size: 5851 AhdI GACnn_n'nnGTC Cuts at: 4580 4580 Size: 5851 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5851 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5851 AlwNI CAG_nnn'CTG Cuts at: 2502 3594 5166 2502 Size: 1092 1572 3187 Fragments arranged by size: 3187 1572 1092 ApaI G_GGCC'C Cuts at: 2013 2013 Size: 5851 ApaLI G'TGCA_C Cuts at: 3908 5064 3908 Size: 1156 4695 ApoI r'AATT_y Cuts at: 1493 2228 3020 3050 3160 3386 3538 5647 1493 Size: 735 792 30 110 226 152 2109 1697 Fragments arranged by size: 2109 1697 792 735 226 152 110 30 AseI AT'TA_AT Cuts at: 160 1032 4405 160 Size: 872 3373 1606 Fragments arranged by size: 3373 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 AvaI C'yCGr_G Cuts at: 1870 1943 2998 3176 1870 Size: 73 1055 178 4545 Fragments arranged by size: 4545 1055 178 73 BamHI G'GATC_C Cuts at: 3181 3548 3181 Size: 367 5484 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2812 2918 3172 4627 618 Size: 325 217 627 1025 106 254 1455 1842 Fragments arranged by size: 1842 1455 1025 627 325 254 217 106 BanII G_rGCy'C Cuts at: 1391 2013 3170 1391 Size: 622 1157 4072 Fragments arranged by size: 4072 1157 622 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2623 2788 2864 3117 928 Size: 971 724 165 76 253 3662 Fragments arranged by size: 3662 971 724 253 165 76 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4042 4076 1903 Size: 34 2105 34 3678 Fragments arranged by size: 3678 2105 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3743 4953 3743 Size: 1210 4641 BclI T'GATC_A Cuts at: 1548 1548 Size: 5851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2810 4462 136 Size: 107 122 71 631 605 1138 1652 1525 Fragments arranged by size: 1652 1525 1138 631 605 122 107 71 BglII A'GATC_T Cuts at: 5846 5846 Size: 5851 BlpI GC'TnA_GC Cuts at: 3268 3268 Size: 5851 Bme1580I G_kGCm'C Cuts at: 1790 2013 2815 2999 3912 5068 1790 Size: 223 802 184 913 1156 2573 Fragments arranged by size: 2573 1156 913 802 223 184 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5851 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2344 3582 4540 Size: 416 53 83 399 24 927 1238 958 Cuts at: 4540 442 Size: 1753 Fragments arranged by size: 1753 1238 958 927 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 4511 1239 Size: 968 2304 2579 Fragments arranged by size: 2579 2304 968 Bpu10I CC'TnA_GC Cuts at: 2789 2789 Size: 5851 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2753 3976 4841 5139 2753 Size: 1223 865 298 3465 Fragments arranged by size: 3465 1223 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2056 3107 4514 882 Size: 617 557 1051 1407 2219 Fragments arranged by size: 2219 1407 1051 617 557 BsaAI yAC'GTr Cuts at: 493 1204 3642 493 Size: 711 2438 2702 Fragments arranged by size: 2702 2438 711 BsaBI GATnn'nnATC Cuts at: 5845 5845 Size: 5851 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3658 4040 4675 275 Size: 53 83 186 936 2125 382 635 1451 Fragments arranged by size: 2125 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2973 4284 4682 4956 5103 5514 2973 Size: 1311 398 274 147 411 3310 Fragments arranged by size: 3310 1311 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3062 3092 1178 Size: 30 165 30 1659 30 3937 Fragments arranged by size: 3937 1659 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2399 1186 Size: 1213 4638 BseYI C'CCAG_C Cuts at: 2505 2808 5054 5550 2505 Size: 303 2246 496 2806 Fragments arranged by size: 2806 2246 496 303 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2605 1163 Size: 573 869 4409 Fragments arranged by size: 4409 869 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3301 4062 5090 1540 Size: 275 186 1300 761 1028 2301 Fragments arranged by size: 2301 1300 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2910 3912 3997 5068 2910 Size: 1002 85 1071 3693 Fragments arranged by size: 3693 1071 1002 85 BsmI GAATG_Cn' Cuts at: 3362 3455 3362 Size: 93 5758 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2815 2910 2999 3170 3912 3997 Size: 399 223 802 95 89 171 742 85 Cuts at: 3997 5068 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 802 742 399 223 171 95 89 85 BspEI T'CCGG_A Cuts at: 2973 5514 2973 Size: 2541 3310 BspHI T'CATG_A Cuts at: 3740 5470 3740 Size: 1730 4121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2316 3206 844 Size: 1472 890 3489 Fragments arranged by size: 3489 1472 890 BsrBI CCG'CTC Cuts at: 1394 1823 2593 2818 2882 3003 3738 1394 Size: 429 770 225 64 121 735 3507 Fragments arranged by size: 3507 770 735 429 225 121 64 BsrDI GCAATG_nn' Cuts at: 65 1248 4346 4520 65 Size: 1183 3098 174 1396 Fragments arranged by size: 3098 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3041 4495 4682 1700 Size: 110 1231 1454 187 2869 Fragments arranged by size: 2869 1454 1231 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5851 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5851 BssSI C'ACGA_G Cuts at: 3911 4923 3911 Size: 1012 4839 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5851 BstBI TT'CG_AA Cuts at: 4655 4655 Size: 5851 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5851 BstYI r'GATC_y Cuts at: 1972 3181 3548 3933 3950 5402 5488 5500 5846 Size: 1209 367 385 17 1452 86 12 346 Cuts at: 5846 1972 Size: 1977 Fragments arranged by size: 1977 1452 1209 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2625 513 Size: 552 939 621 3739 Fragments arranged by size: 3739 939 621 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2401 2785 3022 487 Size: 683 56 1175 384 237 3316 Fragments arranged by size: 3316 1175 683 384 237 56 BtsI GCAGTG_nn' Cuts at: 3367 4161 4181 3367 Size: 794 20 5037 Fragments arranged by size: 5037 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5851 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5816 DraI TTT'AAA Cuts at: 3155 3155 Size: 5851 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3121 4858 809 Size: 507 1805 1737 1802 Fragments arranged by size: 1805 1802 1737 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3298 4187 5816 Size: 54 1425 50 275 18 1468 889 1629 Cuts at: 5816 8 Size: 43 Fragments arranged by size: 1629 1468 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3298 1537 Size: 275 1486 4090 Fragments arranged by size: 4090 1486 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2489 2621 2825 2879 3148 3781 1353 Size: 1136 132 204 54 269 633 3423 Fragments arranged by size: 3423 1136 633 269 204 132 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2468 4438 4812 4958 387 Size: 1064 138 879 1970 374 146 1280 Fragments arranged by size: 1970 1280 1064 879 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2860 3914 4511 5298 5566 1239 Size: 393 575 653 1054 597 787 268 1524 Fragments arranged by size: 1524 1054 787 653 597 575 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2686 2686 Size: 5851 EcoO109I rG'GnC_Cy Cuts at: 2009 2009 Size: 5851 EcoRI G'AATT_C Cuts at: 3050 3160 3538 3050 Size: 110 378 5363 Fragments arranged by size: 5363 378 110 EcoRV GAT'ATC Cuts at: 2074 2074 Size: 5851 FspI TGC'GCA Cuts at: 4357 4357 Size: 5851 HaeII r_GCGC'y Cuts at: 1678 2431 2502 2765 4998 1678 Size: 753 71 263 2233 2531 Fragments arranged by size: 2531 2233 753 263 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3021 3053 3062 3094 4492 Size: 32 135 32 1449 32 9 32 1398 Cuts at: 4492 4524 4566 4598 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1449 1398 135 42 32 32 32 32 32 32 9 HincII GTy'rAC Cuts at: 669 1657 1938 2065 3139 3195 3441 669 Size: 988 281 127 1074 56 246 3079 Fragments arranged by size: 3079 1074 988 281 246 127 56 HindIII A'AGCT_T Cuts at: 748 748 Size: 5851 HpaI GTT'AAC Cuts at: 3441 3441 Size: 5851 KpnI G_GTAC'C Cuts at: 3176 3176 Size: 5851 MfeI C'AATT_G Cuts at: 3450 3450 Size: 5851 MluI A'CGCG_T Cuts at: 5727 5727 Size: 5851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4965 5149 1305 Size: 3660 184 2007 Fragments arranged by size: 3660 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4029 Fragments arranged by size: 4029 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3809 4168 4327 518 Size: 648 2643 359 159 2042 Fragments arranged by size: 2643 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2574 3267 3944 5092 5337 1606 Size: 420 548 693 677 1148 245 2120 Fragments arranged by size: 2120 1148 693 677 548 420 245 NaeI GCC'GGC Cuts at: 3043 3043 Size: 5851 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4360 Fragments arranged by size: 4360 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5851 NgoMIV G'CCGG_C Cuts at: 3041 3041 Size: 5851 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5851 NotI GC'GGCC_GC Cuts at: 3298 3298 Size: 5851 NspI r_CATG'y Cuts at: 1132 1229 3209 4754 1132 Size: 97 1980 1545 2229 Fragments arranged by size: 2229 1980 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5851 PciI A'CATG_T Cuts at: 4750 4750 Size: 5851 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5851 PfoI T'CCnGG_A Cuts at: 2184 2184 Size: 5851 PmeI GTTT'AAAC Cuts at: 3155 3155 Size: 5851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3920 3952 5433 5465 3920 Size: 32 1481 32 4306 Fragments arranged by size: 4306 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5851 PsiI TTA'TAA Cuts at: 3421 3421 Size: 5851 PspOMI G'GGCC_C Cuts at: 2009 2009 Size: 5851 PstI C_TGCA'G Cuts at: 830 2672 3203 830 Size: 1842 531 3478 Fragments arranged by size: 3478 1842 531 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5851 PvuII CAG'CTG Cuts at: 2574 2574 Size: 5851 SalI G'TCGA_C Cuts at: 1936 3193 1936 Size: 1257 4594 SapI GCTCTTCn'nnn_ Cuts at: 2825 2825 Size: 5851 SbfI CC_TGCA'GG Cuts at: 3203 3203 Size: 5851 ScaI AGT'ACT Cuts at: 2888 4099 5811 2888 Size: 1211 1712 2928 Fragments arranged by size: 2928 1712 1211 SfcI C'TryA_G Cuts at: 826 1045 2668 3199 4334 5015 5206 826 Size: 219 1623 531 1135 681 191 1471 Fragments arranged by size: 1623 1471 1135 681 531 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5851 SmaI CCC'GGG Cuts at: 1872 3178 1872 Size: 1306 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2768 3955 4856 5118 820 Size: 197 926 825 1187 901 262 1553 Fragments arranged by size: 1553 1187 926 901 825 262 197 SnaBI TAC'GTA Cuts at: 493 3642 493 Size: 3149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5851 SphI G_CATG'C Cuts at: 1132 3209 1132 Size: 2077 3774 SspI AAT'ATT Cuts at: 5 52 3775 5 Size: 47 3723 2081 Fragments arranged by size: 3723 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5851 StyI C'CwwG_G Cuts at: 513 1065 2004 2682 2730 3290 513 Size: 552 939 678 48 560 3074 Fragments arranged by size: 3074 939 678 560 552 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2038 2516 3878 3895 4048 4233 4678 Size: 1255 458 478 1362 17 153 185 445 Cuts at: 4678 325 Size: 1498 Fragments arranged by size: 1498 1362 1255 478 458 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2244 2664 2886 Size: 274 80 33 51 1443 267 420 222 Cuts at: 2886 4097 5809 96 Size: 1211 1712 138 Fragments arranged by size: 1712 1443 1211 420 274 267 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5811 5843 5811 Size: 32 5819 Tth111I GACn'n_nGTC Cuts at: 1316 2060 1316 Size: 744 5107 XbaI T'CTAG_A Cuts at: 3187 3187 Size: 5851 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2023 2023 Size: 5851 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5851 XmaI C'CCGG_G Cuts at: 1870 3176 1870 Size: 1306 4545 XmnI GAAnn'nnTTC Cuts at: 3980 3980 Size: 5851 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3659 276 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI