Restriction map for
pFN21AB5508
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbrPI BbsI
BbvCI BclI BfaI BfrI BlnI
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI HincII HindII HindIII HpaI
KpnI MaeI MaeII MamI MfeI
MluI MroI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SfcI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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BalI 1523 TGGCCA TGG^CCA
BciVI 467 GTATCC GTATCC(6/5)
BglI 1610 GCCACAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 1225 GTGCAG GTGCAG(16/14)
BsiEI 515 CGGCCG CG[GA][CT]^CG
BsmAI 673 GAGAC GTCTC(1/5)
BsmBI 673 GAGACG CGTCTC(1/5)
BssHII 701 GCGCGC G^CGCGC
BssSI 787 CTCGTG CACGAG(-5/-1)
BstXI 176 CCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 515 CGGCCG C^GGCCG
EclXI 515 CGGCCG C^GGCCG
Eco52I 515 CGGCCG C^GGCCG
FseI 189 GGCCGGCC GGCCGG^CC
HinfI 1023 GATTC G^A[ACGT]TC
KspI 498 CCGCGG CCGC^GG
MluNI 1523 TGGCCA TGG^CCA
MscI 1523 TGGCCA TGG^CCA
NspI 1496 GCATGC [GA]CATG^[CT]
PflMI 1415 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 498 CCGCGG CCGC^GG
SexAI 887 ACCTGGT A^CC[AT]GGT
SmaI 462 CCCGGG CCC^GGG
SmlI 73 CTCAAG C^T[CT][GA]AG
SphI 1496 GCATGC GCATG^C
TfiI 1023 GATTC G^A[AT]TC
Van91I 1415 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 462 CCCGGG C^CCGGG
XmaI 462 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 694 GTCTAC GT^[AC][GT]AC
754 GTCTAC
AlwI 639 GATCC GGATC(4/5)
993 GATCC
ApaI 899 GGGCCC GGGCC^C
1257 GGGCCC
AviII 570 TGCGCA TGC^GCA
872 TGCGCA
BbeI 803 GGCGCC GGCGC^C
1380 GGCGCC
BcgI 92 CGAAGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
332 GCAACGCCCTCG
BglII 584 AGATCT A^GATCT
1364 AGATCT
BlpI 1398 GCTGAGC GC^T[ACGT]AGC
1473 GCTCAGC
Bpu1102I 1398 GCTGAGC GC^T[ACGT]AGC
1473 GCTCAGC
BspMI 230 ACCTGC ACCTGC(4/8)
1574 ACCTGC
BsrBI 1270 GAGCGG CCGCTC(-3/-3)
1401 GAGCGG
BstEII 60 GGTGACC G^GT[ACGT]ACC
1335 GGTGACC
BstPI 60 GGTGACC G^GT[ACGT]ACC
1335 GGTGACC
CelII 1398 GCTGAGC GC^T[ACGT]AGC
1473 GCTCAGC
EcoNI 939 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1533 CCTCATCGAGG
EcoO65I 60 GGTGACC G^GT[ACGT]ACC
1335 GGTGACC
FspI 570 TGCGCA TGC^GCA
872 TGCGCA
KasI 803 GGCGCC G^GCGCC
1380 GGCGCC
MaeIII 61 GTGAC ^GT[ACGT]AC
1336 GTGAC
MslI 1212 CACCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1248 CACCCACATG
NaeI 141 GCCGGC GCC^GGC
190 GCCGGC
NarI 803 GGCGCC GG^CGCC
1380 GGCGCC
NcoI 176 CCATGG C^CATGG
1289 CCATGG
NgoMIV 141 GCCGGC G^CCGGC
190 GCCGGC
SfoI 803 GGCGCC GGC^GCC
1380 GGCGCC
Tsp45I 61 GTGAC ^GT[GC]AC
1336 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI Ksp632I MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI TspRI TthHB8I XcmI
XhoII