Restriction map for
pFN21AB5508
(Linear) (Six-base) MAPSORT of: KIBB5508.seq Check: 41 from: 1 to: 1629
KIBB5508.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AccI GT'mk_AC
Cuts at: 0 695 755 1629
Size: 695 60 874
Fragments arranged by size:
874 695 60
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 961 1141 1629
Size: 34 927 180 488
Fragments arranged by size:
927 488 180 34
AleI CACnn'nnGTG
Cuts at: 0 1216 1629
Size: 1216 413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 112 1629
Size: 112 1517
AlwNI CAG_nnn'CTG
Cuts at: 0 231 435 684 852 1083 1629
Size: 231 204 249 168 231 546
Fragments arranged by size:
546 249 231 231 204 168
ApaI G_GGCC'C
Cuts at: 0 903 1261 1629
Size: 903 358 368
Fragments arranged by size:
903 368 358
AvaI C'yCGr_G
Cuts at: 0 43 462 739 1629
Size: 43 419 277 890
Fragments arranged by size:
890 419 277 43
BanI G'GyrC_C
Cuts at: 0 246 319 478 803 1246 1380 1590 1629
Size: 246 73 159 325 443 134 210 39
Fragments arranged by size:
443 325 246 210 159 134 73 39
BanII G_rGCy'C
Cuts at: 0 275 903 1261 1312 1621 1629
Size: 275 628 358 51 309 8
Fragments arranged by size:
628 358 309 275 51 8
BbeI G_GCGC'C
Cuts at: 0 807 1384 1629
Size: 807 577 245
Fragments arranged by size:
807 577 245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 81 115 321 355 1629
Size: 81 34 206 34 1274
Fragments arranged by size:
1274 206 81 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 478 1629
Size: 478 1151
BglI GCCn_nnn'nGGC
Cuts at: 0 1616 1629
Size: 1616 13
BglII A'GATC_T
Cuts at: 0 584 1364 1629
Size: 584 780 265
Fragments arranged by size:
780 584 265
BlpI GC'TnA_GC
Cuts at: 0 1399 1474 1629
Size: 1399 75 155
Fragments arranged by size:
1399 155 75
Bme1580I G_kGCm'C
Cuts at: 0 30 251 322 329 417 736 794 903
Size: 30 221 71 7 88 319 58 109
Cuts at: 903 1249 1261 1629
Size: 346 12 368
Fragments arranged by size:
368 346 319 221 109 88 71 58
30 12 7
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 972 1326 1629
Size: 972 354 303
Fragments arranged by size:
972 354 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 169 256 457 601 838 1156 1225
Size: 127 42 87 201 144 237 318 69
Cuts at: 1225 1629
Size: 404
Fragments arranged by size:
404 318 237 201 144 127 87 69
42
Bpu10I CC'TnA_GC
Cuts at: 0 269 1629
Size: 269 1360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1629
Size: 58 1571
BsaHI Gr'CG_yC
Cuts at: 0 804 1381 1608 1629
Size: 804 577 227 21
Fragments arranged by size:
804 577 227 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 748 778 1603 1629
Size: 748 30 825 26
Fragments arranged by size:
825 748 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 775 835 1213 1318 1629
Size: 124 651 60 378 105 311
Fragments arranged by size:
651 378 311 124 105 60
BseYI C'CCAG_C
Cuts at: 0 416 717 776 1455 1629
Size: 416 301 59 679 174
Fragments arranged by size:
679 416 301 174 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1246 1629
Size: 1246 383
BsiEI CG_ry'CG
Cuts at: 0 518 1629
Size: 518 1111
BsiHKAI G_wGCw'C
Cuts at: 0 116 653 1213 1629
Size: 116 537 560 416
Fragments arranged by size:
560 537 416 116
BsmBI CGTCTCn'nnnn_
Cuts at: 0 667 1629
Size: 667 962
Bsp1286I G_dGCh'C
Cuts at: 0 30 116 251 275 322 329 417 653
Size: 30 86 135 24 47 7 88 236
Cuts at: 653 736 794 903 1213 1249 1261 1312 1621
Size: 83 58 109 310 36 12 51 309
Cuts at: 1621 1629
Size: 8
Fragments arranged by size:
310 309 236 135 109 88 86 83
58 51 47 36 30 24 12 8
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 1583 1629
Size: 239 1344 46
Fragments arranged by size:
1344 239 46
BsrBI CCG'CTC
Cuts at: 0 1272 1403 1629
Size: 1272 131 226
Fragments arranged by size:
1272 226 131
BsrFI r'CCGG_y
Cuts at: 0 141 190 800 1629
Size: 141 49 610 829
Fragments arranged by size:
829 610 141 49
BssHII G'CGCG_C
Cuts at: 0 701 1629
Size: 701 928
BssSI C'ACGA_G
Cuts at: 0 787 1629
Size: 787 842
BstAPI GCAn_nnn'nTGC
Cuts at: 0 246 687 1083 1629
Size: 246 441 396 546
Fragments arranged by size:
546 441 396 246
BstEII G'GTnAC_C
Cuts at: 0 60 1335 1629
Size: 60 1275 294
Fragments arranged by size:
1275 294 60
BstXI CCAn_nnnn'nTGG
Cuts at: 0 183 1629
Size: 183 1446
BstYI r'GATC_y
Cuts at: 0 584 638 1364 1629
Size: 584 54 726 265
Fragments arranged by size:
726 584 265 54
BtgI C'CryG_G
Cuts at: 0 176 498 1289 1557 1629
Size: 176 322 791 268 72
Fragments arranged by size:
791 322 268 176 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1152 1493 1629
Size: 1152 341 136
Fragments arranged by size:
1152 341 136
EaeI y'GGCC_r
Cuts at: 0 188 192 293 515 1523 1629
Size: 188 4 101 222 1008 106
Fragments arranged by size:
1008 222 188 106 101 4
EagI C'GGCC_G
Cuts at: 0 515 1629
Size: 515 1114
EarI CTCTTCn'nnn_
Cuts at: 0 814 838 889 1505 1629
Size: 814 24 51 616 124
Fragments arranged by size:
814 616 124 51 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1611 1629
Size: 1611 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 127 169 256 457 601 838 961
Size: 34 93 42 87 201 144 237 123
Cuts at: 961 1141 1156 1225 1629
Size: 180 15 69 404
Fragments arranged by size:
404 237 201 180 144 123 93 87
69 42 34 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 943 1537 1629
Size: 943 594 92
Fragments arranged by size:
943 594 92
EcoO109I rG'GnC_Cy
Cuts at: 0 128 265 432 828 849 899 1276 1629
Size: 128 137 167 396 21 50 377 353
Fragments arranged by size:
396 377 353 167 137 128 50 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 234 1629
Size: 234 1395
FseI GG_CCGG'CC
Cuts at: 0 194 1629
Size: 194 1435
FspI TGC'GCA
Cuts at: 0 572 874 1629
Size: 572 302 755
Fragments arranged by size:
755 572 302
FspAI rTGC'GCAy
Cuts at: 0 572 1629
Size: 572 1057
HaeII r_GCGC'y
Cuts at: 0 718 807 1238 1384 1629
Size: 718 89 431 146 245
Fragments arranged by size:
718 431 245 146 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 583 615 1629
Size: 583 32 1014
Fragments arranged by size:
1014 583 32
KasI G'GCGC_C
Cuts at: 0 803 1380 1629
Size: 803 577 249
Fragments arranged by size:
803 577 249
MscI TGG'CCA
Cuts at: 0 1525 1629
Size: 1525 104
MslI CAynn'nnrTG
Cuts at: 0 1216 1252 1629
Size: 1216 36 377
Fragments arranged by size:
1216 377 36
MspA1I CmG'CkG
Cuts at: 0 8 105 500 684 990 1029 1629
Size: 8 97 395 184 306 39 600
Fragments arranged by size:
600 395 306 184 97 39 8
NaeI GCC'GGC
Cuts at: 0 143 192 1629
Size: 143 49 1437
Fragments arranged by size:
1437 143 49
NarI GG'CG_CC
Cuts at: 0 804 1381 1629
Size: 804 577 248
Fragments arranged by size:
804 577 248
NcoI C'CATG_G
Cuts at: 0 176 1289 1629
Size: 176 1113 340
Fragments arranged by size:
1113 340 176
NgoMIV G'CCGG_C
Cuts at: 0 141 190 1629
Size: 141 49 1439
Fragments arranged by size:
1439 141 49
NspI r_CATG'y
Cuts at: 0 1500 1629
Size: 1500 129
PasI CC'CwG_GG
Cuts at: 0 1048 1629
Size: 1048 581
PflMI CCAn_nnn'nTGG
Cuts at: 0 1421 1629
Size: 1421 208
PfoI T'CCnGG_A
Cuts at: 0 434 1629
Size: 434 1195
PpuMI rG'GwC_Cy
Cuts at: 0 432 828 1276 1629
Size: 432 396 448 353
Fragments arranged by size:
448 432 396 353
PspOMI G'GGCC_C
Cuts at: 0 899 1257 1629
Size: 899 358 372
Fragments arranged by size:
899 372 358
PvuII CAG'CTG
Cuts at: 0 8 684 990 1029 1629
Size: 8 676 306 39 600
Fragments arranged by size:
676 600 306 39 8
SacII CC_GC'GG
Cuts at: 0 501 1629
Size: 501 1128
SexAI A'CCwGG_T
Cuts at: 0 887 1629
Size: 887 742
SfoI GGC'GCC
Cuts at: 0 805 1382 1629
Size: 805 577 247
Fragments arranged by size:
805 577 247
SmaI CCC'GGG
Cuts at: 0 464 1629
Size: 464 1165
SmlI C'TyrA_G
Cuts at: 0 73 1629
Size: 73 1556
SphI G_CATG'C
Cuts at: 0 1500 1629
Size: 1500 129
StyI C'CwwG_G
Cuts at: 0 176 1289 1340 1409 1629
Size: 176 1113 51 69 220
Fragments arranged by size:
1113 220 176 69 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1264 1320 1629
Size: 1264 56 309
Fragments arranged by size:
1264 309 56
TatI w'GTAC_w
Cuts at: 0 197 1629
Size: 197 1432
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1603 1629
Size: 1603 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 183 1422 1452 1629
Size: 183 1239 30 177
Fragments arranged by size:
1239 183 177 30
XmaI C'CCGG_G
Cuts at: 0 462 1629
Size: 462 1167
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BcgI BciVI BglI BglII BlpI Bme1580I
BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI
FseI FspI FspAI HaeII Hin4I KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PpuMI PspOMI PvuII SacII SexAI SfoI SmaI
SmlI SphI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BclI BfrBI BmgBI BmrI
BmtI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BstBI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV HincII
HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmnI ZraI