Restriction map for
pFN21AB5493
(Linear) (Six-base) MAPSORT of: KIBB5493.seq Check: 7906 from: 1 to: 2724
KIBB5493.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 627 1534 2724
Size: 627 907 1190
Fragments arranged by size:
1190 907 627
Acc65I G'GTAC_C
Cuts at: 0 2203 2724
Size: 2203 521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 254 337 809 1600 1840 2724
Size: 158 96 83 472 791 240 884
Fragments arranged by size:
884 791 472 240 158 96 83
AfeI AGC'GCT
Cuts at: 0 221 2724
Size: 221 2503
AleI CACnn'nnGTG
Cuts at: 0 559 1780 2724
Size: 559 1221 944
Fragments arranged by size:
1221 944 559
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 702 2724
Size: 702 2022
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1102 1134 1943 1975 2724
Size: 1102 32 809 32 749
Fragments arranged by size:
1102 809 749 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 132 516 1642 1807 1833 2301 2527 2724
Size: 132 384 1126 165 26 468 226 197
Fragments arranged by size:
1126 468 384 226 197 165 132 26
ApoI r'AATT_y
Cuts at: 0 289 2134 2724
Size: 289 1845 590
Fragments arranged by size:
1845 590 289
AvaI C'yCGr_G
Cuts at: 0 1001 2724
Size: 1001 1723
BanI G'GyrC_C
Cuts at: 0 24 1029 2203 2320 2724
Size: 24 1005 1174 117 404
Fragments arranged by size:
1174 1005 404 117 24
BanII G_rGCy'C
Cuts at: 0 439 1025 1332 1649 2724
Size: 439 586 307 317 1075
Fragments arranged by size:
1075 586 439 317 307
BbeI G_GCGC'C
Cuts at: 0 28 2724
Size: 28 2696
BbsI GAAGACnn'nnnn_
Cuts at: 0 842 1886 2584 2724
Size: 842 1044 698 140
Fragments arranged by size:
1044 842 698 140
BciVI GTATCCnnnnn_n'
Cuts at: 0 192 1647 2115 2724
Size: 192 1455 468 609
Fragments arranged by size:
1455 609 468 192
BfrBI ATG'CAT
Cuts at: 0 673 2724
Size: 673 2051
BglI GCCn_nnn'nGGC
Cuts at: 0 1720 2221 2567 2724
Size: 1720 501 346 157
Fragments arranged by size:
1720 501 346 157
Bme1580I G_kGCm'C
Cuts at: 0 278 756 2323 2724
Size: 278 478 1567 401
Fragments arranged by size:
1567 478 401 278
BmrI ACTGGGnnnn_n'
Cuts at: 0 1693 2605 2724
Size: 1693 912 119
Fragments arranged by size:
1693 912 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 187 244 670 934 1759 2724
Size: 32 155 57 426 264 825 965
Fragments arranged by size:
965 825 426 264 155 57 32
Bpu10I CC'TnA_GC
Cuts at: 0 500 722 996 2128 2724
Size: 500 222 274 1132 596
Fragments arranged by size:
1132 596 500 274 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1633 2724
Size: 403 1230 1091
Fragments arranged by size:
1230 1091 403
BsaI GGTCTCn'nnnn_
Cuts at: 0 1693 2724
Size: 1693 1031
BsaAI yAC'GTr
Cuts at: 0 717 2724
Size: 717 2007
BsaBI GATnn'nnATC
Cuts at: 0 2150 2724
Size: 2150 574
BsaHI Gr'CG_yC
Cuts at: 0 25 2724
Size: 25 2699
BsaWI w'CCGG_w
Cuts at: 0 1364 2057 2724
Size: 1364 693 667
Fragments arranged by size:
1364 693 667
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 361 391 397 427 1102 1132 1285 1315
Size: 361 30 6 30 675 30 153 30
Cuts at: 1315 1732 1762 1846 1876 1943 1973 2724
Size: 417 30 84 30 67 30 751
Fragments arranged by size:
751 675 417 361 153 84 67 30
30 30 30 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 949 1110 1293 2005 2518 2668 2724
Size: 19 930 161 183 712 513 150 56
Fragments arranged by size:
930 712 513 183 161 150 56 19
BseYI C'CCAG_C
Cuts at: 0 38 2724
Size: 38 2686
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 1247 1718 1846 2021 2724
Size: 52 1195 471 128 175 703
Fragments arranged by size:
1195 703 471 175 128 52
BsiEI CG_ry'CG
Cuts at: 0 2352 2724
Size: 2352 372
BsiHKAI G_wGCw'C
Cuts at: 0 1332 1649 1944 2724
Size: 1332 317 295 780
Fragments arranged by size:
1332 780 317 295
BsmI GAATG_Cn'
Cuts at: 0 883 1149 2489 2724
Size: 883 266 1340 235
Fragments arranged by size:
1340 883 266 235
Bsp1286I G_dGCh'C
Cuts at: 0 278 439 756 1025 1332 1649 1944 2323
Size: 278 161 317 269 307 317 295 379
Cuts at: 2323 2724
Size: 401
Fragments arranged by size:
401 379 317 317 307 295 278 269
161
BspEI T'CCGG_A
Cuts at: 0 1364 2057 2724
Size: 1364 693 667
Fragments arranged by size:
1364 693 667
BspHI T'CATG_A
Cuts at: 0 465 2142 2724
Size: 465 1677 582
Fragments arranged by size:
1677 582 465
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 626 627 1534 2724
Size: 626 1 907 1190
Fragments arranged by size:
1190 907 626 1
BsrBI CCG'CTC
Cuts at: 0 86 96 2724
Size: 86 10 2628
Fragments arranged by size:
2628 86 10
BsrDI GCAATG_nn'
Cuts at: 0 1596 2234 2515 2724
Size: 1596 638 281 209
Fragments arranged by size:
1596 638 281 209
BsrFI r'CCGG_y
Cuts at: 0 27 896 2351 2724
Size: 27 869 1455 373
Fragments arranged by size:
1455 869 373 27
BsrGI T'GTAC_A
Cuts at: 0 969 2724
Size: 969 1755
BssSI C'ACGA_G
Cuts at: 0 175 2724
Size: 175 2549
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1833 2724
Size: 1833 891
BstBI TT'CG_AA
Cuts at: 0 2418 2724
Size: 2418 306
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1779 2190 2724
Size: 1779 411 534
Fragments arranged by size:
1779 534 411
BtgI C'CryG_G
Cuts at: 0 1212 2183 2375 2625 2724
Size: 1212 971 192 250 99
Fragments arranged by size:
1212 971 250 192 99
BtsI GCAGTG_nn'
Cuts at: 0 1086 1390 1495 2205 2552 2724
Size: 1086 304 105 710 347 172
Fragments arranged by size:
1086 710 347 304 172 105
EaeI y'GGCC_r
Cuts at: 0 386 1155 2025 2372 2512 2645 2724
Size: 386 769 870 347 140 133 79
Fragments arranged by size:
870 769 386 347 140 133 79
EarI CTCTTCn'nnn_
Cuts at: 0 134 1337 2281 2724
Size: 134 1203 944 443
Fragments arranged by size:
1203 944 443 134
EcoICRI GAG'CTC
Cuts at: 0 1330 1647 2724
Size: 1330 317 1077
Fragments arranged by size:
1330 1077 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 158 187 244 254 337 670 809
Size: 32 126 29 57 10 83 333 139
Cuts at: 809 934 1600 1759 1840 2724
Size: 125 666 159 81 884
Fragments arranged by size:
884 666 333 159 139 126 125 83
81 57 32 29 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1702 2724
Size: 1702 1022
EcoO109I rG'GnC_Cy
Cuts at: 0 945 1583 2262 2298 2724
Size: 945 638 679 36 426
Fragments arranged by size:
945 679 638 426 36
HaeII r_GCGC'y
Cuts at: 0 28 223 2724
Size: 28 195 2501
Fragments arranged by size:
2501 195 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 946 978 1510 1542 1673 1705
Size: 361 32 553 32 532 32 131 32
Cuts at: 1705 1732 1764 2099 2131 2724
Size: 27 32 335 32 593
Fragments arranged by size:
593 553 532 361 335 131 32 32
32 32 32 32 27
KasI G'GCGC_C
Cuts at: 0 24 2724
Size: 24 2700
KpnI G_GTAC'C
Cuts at: 0 2207 2724
Size: 2207 517
MscI TGG'CCA
Cuts at: 0 388 1157 2027 2647 2724
Size: 388 769 870 620 77
Fragments arranged by size:
870 769 620 388 77
MslI CAynn'nnrTG
Cuts at: 0 394 470 559 1537 1780 2188 2724
Size: 394 76 89 978 243 408 536
Fragments arranged by size:
978 536 408 394 243 89 76
MspA1I CmG'CkG
Cuts at: 0 52 540 1231 1833 2724
Size: 52 488 691 602 891
Fragments arranged by size:
891 691 602 488 52
NaeI GCC'GGC
Cuts at: 0 898 2724
Size: 898 1826
NarI GG'CG_CC
Cuts at: 0 25 2724
Size: 25 2699
NcoI C'CATG_G
Cuts at: 0 2183 2724
Size: 2183 541
NdeI CA'TA_TG
Cuts at: 0 675 1797 2724
Size: 675 1122 927
Fragments arranged by size:
1122 927 675
NgoMIV G'CCGG_C
Cuts at: 0 896 2724
Size: 896 1828
NsiI A_TGCA'T
Cuts at: 0 675 2724
Size: 675 2049
NspI r_CATG'y
Cuts at: 0 648 2724
Size: 648 2076
PasI CC'CwG_GG
Cuts at: 0 343 756 2724
Size: 343 413 1968
Fragments arranged by size:
1968 413 343
PflMI CCAn_nnn'nTGG
Cuts at: 0 307 2547 2724
Size: 307 2240 177
Fragments arranged by size:
2240 307 177
PfoI T'CCnGG_A
Cuts at: 0 911 2117 2408 2724
Size: 911 1206 291 316
Fragments arranged by size:
1206 911 316 291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1102 1134 1422 1454 1943 1975 2724
Size: 1102 32 288 32 489 32 749
Fragments arranged by size:
1102 749 489 288 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 945 1583 2262 2724
Size: 945 638 679 462
Fragments arranged by size:
945 679 638 462
PvuII CAG'CTG
Cuts at: 0 1231 1833 2724
Size: 1231 602 891
Fragments arranged by size:
1231 891 602
SacI G_AGCT'C
Cuts at: 0 1332 1649 2724
Size: 1332 317 1075
Fragments arranged by size:
1332 1075 317
SapI GCTCTTCn'nnn_
Cuts at: 0 1337 2724
Size: 1337 1387
SfoI GGC'GCC
Cuts at: 0 26 2724
Size: 26 2698
SgrAI Cr'CCGG_yG
Cuts at: 0 27 2724
Size: 27 2697
SmlI C'TyrA_G
Cuts at: 0 418 1648 2724
Size: 418 1230 1076
Fragments arranged by size:
1230 1076 418
StyI C'CwwG_G
Cuts at: 0 1025 2183 2258 2724
Size: 1025 1158 75 466
Fragments arranged by size:
1158 1025 466 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 748 1294 2724
Size: 748 546 1430
Fragments arranged by size:
1430 748 546
TatI w'GTAC_w
Cuts at: 0 867 969 1298 2724
Size: 867 102 329 1426
Fragments arranged by size:
1426 867 329 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 397 429 1359 1391 1846 1878 2724
Size: 397 32 930 32 455 32 846
Fragments arranged by size:
930 846 455 397 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1685 2724
Size: 1685 1039
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1536 1779 2181 2190 2724
Size: 1536 243 402 9 534
Fragments arranged by size:
1536 534 402 243 9
Enzymes that do cut:
AarI Acc65I AcuI AfeI AleI AlfI AloI AlwNI
ApoI AvaI BanI BanII BbeI BbsI BciVI BfrBI
BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BtgI BtsI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI KpnI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PflMI PfoI PpiI
PpuMI PvuII SacI SapI SfoI SgrAI SmlI StyI
TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BclI BglII BlpI BmgBI BmtI BplI BsiWI
BsmBI BssHII BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI MfeI
MluI MmeI NheI NotI NruI PacI PciI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SgfI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XhoI XmaI XmnI ZraI