Restriction map for
pFN21AB5448
##################################
# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT14I EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeIII
MfeI MluI MluNI MscI MseI
MslI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 11 TCCGGA T^CCGGA
AciI 384 GCGG CCGC(-3/-1)
AfaI 465 GTAC GT^AC
AlwI 357 GGATC GGATC(4/5)
AocI 204 CCTGAGG CC^T[ACGT]AGG
AspHI 373 GAGCAC G[AT]GC[AT]^C
BanI 566 GGTGCC G^G[CT][GA]CC
BbsI 70 GTCTTC GAAGAC(2/6)
BpuAI 70 GTCTTC GAAGAC(2/6)
BsaBI 193 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 11 TCCGGA [AT]^CCGG[AT]
BseAI 11 TCCGGA T^CCGGA
BsiHKAI 373 GAGCAC G[AT]GC[AT]^C
BsiYI 421 CCTGAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 421 CCTGAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspEI 11 TCCGGA T^CCGGA
BspMI 153 GCAGGT ACCTGC(4/8)
BsrFI 411 GCCGGT [GA]^CCGG[CT]
BssKI 339 CCTGG ^CC[ACGT]GG
BstNI 339 CCTGG CC^[AT]GG
BstYI 357 GGATCT [GA]^GATC[CT]
Bsu36I 204 CCTGAGG CC^T[ACGT]AGG
CfoI 455 GCGC GCG^C
Cfr10I 411 GCCGGT [GA]^CCGG[CT]
DdeI 205 CTGAG C^T[ACGT]AG
DraII 208 AGGCCCC [GA]G^G[ACGT]CC[CT]
Eco81I 204 CCTGAGG CC^T[ACGT]AGG
EcoNI 421 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 208 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 339 CCTGG ^CC[AT]GG
EcoRV 133 GATATC GAT^ATC
FauI 384 GCGGG CCCGC(4/6)
HaeII 454 GGCGCT [GA]GCGC^[CT]
HaeIII 209 GGCC GG^CC
HhaI 455 GCGC GCG^C
HinP1I 455 GCGC G^CGC
MamI 193 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 357 GGATCT [GA]^GATC[CT]
MroI 11 TCCGGA T^CCGGA
MspA1I 179 CAGCTG C[AC]G^C[GT]G
MvaI 339 CCTGG CC^[AT]GG
NheI 390 GCTAGC G^CTAGC
NspI 55 ACATGC [GA]CATG^[CT]
PvuII 179 CAGCTG CAG^CTG
RsaI 465 GTAC GT^AC
ScrFI 339 CCTGG CC^[ACGT]GG
TspRI 110 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 357 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 81 GGACC G^G[AT]CC
296 GGACC
BbvI 4 GCTGC GCAGC(8/12)
529 GCTGC
BfaI 391 CTAG C^TAG
542 CTAG
BsmFI 173 GGGAC GGGAC(10/14)
320 GTCCC
BsrI 21 ACTGG ACTGG(1/-1)
84 CCAGT
BstXI 286 CCAGAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
332 CCATCATCCTGG
DpnII 199 GATC ^GATC
358 GATC
Fnu4HI 4 GCTGC GC^[ACGT]GC
529 GCTGC
HapII 12 CCGG C^CGG
412 CCGG
HpaII 12 CCGG C^CGG
412 CCGG
ItaI 4 GCTGC GC^[ACGT]GC
529 GCTGC
MaeI 391 CTAG C^TAG
542 CTAG
MaeII 326 ACGT A^CGT
546 ACGT
MboI 199 GATC ^GATC
358 GATC
MspI 12 CCGG C^CGG
412 CCGG
NdeII 199 GATC ^GATC
358 GATC
NlaIII 56 CATG CATG^
468 CATG
Sau3AI 199 GATC ^GATC
358 GATC
TaqI 137 TCGA T^CGA
257 TCGA
TseI 4 GCTGC G^C[AT]GC
529 GCTGC
TthHB8I 137 TCGA T^CGA
257 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AluI BmyI Bsp1286I BstF5I Cac8I
Cfr13I FokI HphI MboII MnlI
MwoI NlaIV Sau96I