(Circular) (Six-base) MAPSORT of: pFN21AB5432.seq Check: 9787 from: 1 to: 6532 pFN21AB5432 6532 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3367 3367 Size: 6532 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4342 278 Size: 53 83 186 936 2806 2468 Fragments arranged by size: 2806 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3875 1937 Size: 1938 4594 Acc65I G'GTAC_C Cuts at: 2270 3853 2270 Size: 1583 4949 AclI AA'CG_TT Cuts at: 1563 2392 4659 5032 1563 Size: 829 2267 373 3063 Fragments arranged by size: 3063 2267 829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2639 2807 3092 3448 3633 3716 3794 3806 Size: 1007 168 285 356 185 83 78 12 Cuts at: 3806 4595 5979 6247 1632 Size: 789 1384 268 1917 Fragments arranged by size: 1917 1384 1007 789 356 285 268 185 168 83 78 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6335 AflIII A'CryG_T Cuts at: 1203 5431 6408 1203 Size: 4228 977 1327 Fragments arranged by size: 4228 1327 977 AgeI A'CCGG_T Cuts at: 5363 5363 Size: 6532 AhdI GACnn_n'nnGTC Cuts at: 5261 5261 Size: 6532 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6532 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3211 1184 Size: 2027 4505 AlwNI CAG_nnn'CTG Cuts at: 2326 3799 4275 5847 2326 Size: 1473 476 1572 3011 Fragments arranged by size: 3011 1572 1473 476 ApaLI G'TGCA_C Cuts at: 4589 5745 4589 Size: 1156 5376 ApoI r'AATT_y Cuts at: 1493 2287 2296 2349 3276 3841 4067 4219 6328 Size: 794 9 53 927 565 226 152 2109 Cuts at: 6328 1493 Size: 1697 Fragments arranged by size: 2109 1697 927 794 565 226 152 53 9 AseI AT'TA_AT Cuts at: 160 1032 5086 160 Size: 872 4054 1606 Fragments arranged by size: 4054 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 AvaI C'yCGr_G Cuts at: 1870 1943 3857 1870 Size: 73 1914 4545 Fragments arranged by size: 4545 1914 73 BamHI G'GATC_C Cuts at: 3862 4229 3862 Size: 367 6165 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2270 3244 3853 5308 618 Size: 325 217 627 483 974 609 1455 1842 Fragments arranged by size: 1842 1455 974 627 609 483 325 217 BanII G_rGCy'C Cuts at: 1391 3140 3563 3851 1391 Size: 1749 423 288 4072 Fragments arranged by size: 4072 1749 423 288 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2526 2730 3704 928 Size: 971 627 204 974 3756 Fragments arranged by size: 3756 974 971 627 204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4723 4757 1903 Size: 34 2786 34 3678 Fragments arranged by size: 3678 2786 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2213 4424 5634 2213 Size: 2211 1210 3111 Fragments arranged by size: 3111 2211 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6532 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2141 2558 3761 Size: 107 122 71 631 605 469 417 1203 Cuts at: 3761 5143 136 Size: 1382 1525 Fragments arranged by size: 1525 1382 1203 631 605 469 417 122 107 71 BglII A'GATC_T Cuts at: 2245 2964 6527 2245 Size: 719 3563 2250 Fragments arranged by size: 3563 2250 719 BlpI GC'TnA_GC Cuts at: 3949 3949 Size: 6532 Bme1580I G_kGCm'C Cuts at: 1790 3741 4593 5749 1790 Size: 1951 852 1156 2573 Fragments arranged by size: 2573 1951 1156 852 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6532 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3566 4263 5221 Size: 416 53 83 399 24 2149 697 958 Cuts at: 5221 442 Size: 1753 Fragments arranged by size: 2149 1753 958 697 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6532 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3011 3194 3276 3354 3494 5192 1239 Size: 1772 183 82 78 140 1698 2579 Fragments arranged by size: 2579 1772 1698 183 140 82 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2831 4657 5522 5820 2831 Size: 1826 865 298 3543 Fragments arranged by size: 3543 1826 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2747 2964 5195 882 Size: 617 1248 217 2231 2219 Fragments arranged by size: 2231 2219 1248 617 217 BsaAI yAC'GTr Cuts at: 493 1204 4323 493 Size: 711 3119 2702 Fragments arranged by size: 3119 2702 711 BsaBI GATnn'nnATC Cuts at: 2244 3606 6526 2244 Size: 1362 2920 2250 Fragments arranged by size: 2920 2250 1362 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4339 4721 5356 275 Size: 53 83 186 936 2806 382 635 1451 Fragments arranged by size: 2806 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4965 5363 5637 5784 6195 4965 Size: 398 274 147 411 5302 Fragments arranged by size: 5302 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6307 Fragments arranged by size: 6307 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2844 3131 3279 3704 1186 Size: 1658 287 148 425 4014 Fragments arranged by size: 4014 1658 425 287 148 BseYI C'CCAG_C Cuts at: 3721 5735 6231 3721 Size: 2014 496 4022 Fragments arranged by size: 4022 2014 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3217 1163 Size: 573 1481 4478 Fragments arranged by size: 4478 1481 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3982 4743 5771 1540 Size: 275 186 1981 761 1028 2301 Fragments arranged by size: 2301 1981 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2770 3140 3157 4593 4678 5749 2770 Size: 370 17 1436 85 1071 3553 Fragments arranged by size: 3553 1436 1071 370 85 17 BsmI GAATG_Cn' Cuts at: 4043 4136 4043 Size: 93 6439 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2770 3140 3157 3563 3741 3851 4593 Size: 399 980 370 17 406 178 110 742 Cuts at: 4593 4678 5749 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 980 742 406 399 370 178 110 85 17 BspEI T'CCGG_A Cuts at: 6195 6195 Size: 6532 BspHI T'CATG_A Cuts at: 4421 6151 4421 Size: 1730 4802 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2766 3367 3887 844 Size: 1922 601 520 3489 Fragments arranged by size: 3489 1922 601 520 BsrBI CCG'CTC Cuts at: 1394 1823 4419 1394 Size: 429 2596 3507 Fragments arranged by size: 3507 2596 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2542 3524 3549 5027 5201 65 Size: 1183 1294 982 25 1478 174 1396 Fragments arranged by size: 1478 1396 1294 1183 982 174 25 BsrFI r'CCGG_y Cuts at: 1700 1810 2573 5176 5363 1700 Size: 110 763 2603 187 2869 Fragments arranged by size: 2869 2603 763 187 110 BsrGI T'GTAC_A Cuts at: 96 2214 96 Size: 2118 4414 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6532 BssSI C'ACGA_G Cuts at: 4592 5604 4592 Size: 1012 5520 BstAPI GCAn_nnn'nTGC Cuts at: 794 3799 794 Size: 3005 3527 BstBI TT'CG_AA Cuts at: 5336 5336 Size: 6532 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6532 BstYI r'GATC_y Cuts at: 1972 2245 2417 2964 3862 4229 4614 4631 6083 Size: 273 172 547 898 367 385 17 1452 Cuts at: 6083 6169 6181 6527 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 898 547 385 367 346 273 172 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2690 3089 2690 Size: 399 6133 BtgI C'CryG_G Cuts at: 513 1065 2413 2530 513 Size: 552 1348 117 4515 Fragments arranged by size: 4515 1348 552 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2055 487 Size: 683 56 829 4964 Fragments arranged by size: 4964 829 683 56 BtsI GCAGTG_nn' Cuts at: 2242 3519 4048 4842 4862 2242 Size: 1277 529 794 20 3912 Fragments arranged by size: 3912 1277 794 529 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6532 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6497 DraI TTT'AAA Cuts at: 2302 3836 2302 Size: 1534 4998 DraIII CAC_nnn'GTG Cuts at: 2435 2435 Size: 6532 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5539 809 Size: 507 4223 1802 Fragments arranged by size: 4223 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3979 4868 6497 Size: 54 1425 50 275 18 2149 889 1629 Cuts at: 6497 8 Size: 43 Fragments arranged by size: 2149 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3979 1537 Size: 275 2167 4090 Fragments arranged by size: 4090 2167 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2061 2101 3003 3252 3681 3770 4462 1353 Size: 708 40 902 249 429 89 692 3423 Fragments arranged by size: 3423 902 708 692 429 249 89 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5119 5493 5639 387 Size: 1064 138 3530 374 146 1280 Fragments arranged by size: 3530 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3138 3138 Size: 6532 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2639 2807 3011 3092 3194 3276 3354 Size: 393 1007 168 204 81 102 82 78 Cuts at: 3354 3448 3494 3633 3716 3794 3806 4595 5192 Size: 94 46 139 83 78 12 789 597 Cuts at: 5192 5979 6247 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1007 789 787 597 393 268 204 168 139 102 94 83 82 81 78 78 46 12 EcoO109I rG'GnC_Cy Cuts at: 2686 3093 2686 Size: 407 6125 EcoRI G'AATT_C Cuts at: 2349 3841 4219 2349 Size: 1492 378 4662 Fragments arranged by size: 4662 1492 378 EcoRV GAT'ATC Cuts at: 2662 2662 Size: 6532 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2090 3361 3430 2090 Size: 1271 69 5192 Fragments arranged by size: 5192 1271 69 FspI TGC'GCA Cuts at: 5038 5038 Size: 6532 HaeII r_GCGC'y Cuts at: 1678 5679 1678 Size: 4001 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2649 2681 5173 5205 5247 Size: 32 135 32 1077 32 2492 32 42 Cuts at: 5247 5279 1373 Size: 32 2626 Fragments arranged by size: 2626 2492 1077 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2175 3876 4122 669 Size: 988 281 237 1701 246 3079 Fragments arranged by size: 3079 1701 988 281 246 237 HindIII A'AGCT_T Cuts at: 748 3407 748 Size: 2659 3873 HpaI GTT'AAC Cuts at: 4122 4122 Size: 6532 KpnI G_GTAC'C Cuts at: 2274 3857 2274 Size: 1583 4949 MfeI C'AATT_G Cuts at: 4131 4131 Size: 6532 MluI A'CGCG_T Cuts at: 6408 6408 Size: 6532 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3243 5646 5830 1305 Size: 1938 2403 184 2007 Fragments arranged by size: 2403 2007 1938 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4710 Fragments arranged by size: 4710 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2535 3642 4490 4849 5008 518 Size: 648 1369 1107 848 359 159 2042 Fragments arranged by size: 2042 1369 1107 848 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3304 3948 4625 5773 6018 1606 Size: 1698 644 677 1148 245 2120 Fragments arranged by size: 2120 1698 1148 677 644 245 NcoI C'CATG_G Cuts at: 513 1065 2530 513 Size: 552 1465 4515 Fragments arranged by size: 4515 1465 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6532 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6532 NotI GC'GGCC_GC Cuts at: 3979 3979 Size: 6532 NspI r_CATG'y Cuts at: 1132 1229 3890 5435 1132 Size: 97 2661 1545 2229 Fragments arranged by size: 2661 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6532 PciI A'CATG_T Cuts at: 5431 5431 Size: 6532 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6532 PfoI T'CCnGG_A Cuts at: 3825 3825 Size: 6532 PmeI GTTT'AAAC Cuts at: 3836 3836 Size: 6532 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4601 4633 6114 6146 4601 Size: 32 1481 32 4987 Fragments arranged by size: 4987 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2686 2686 Size: 6532 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6532 PsiI TTA'TAA Cuts at: 2826 4102 2826 Size: 1276 5256 PstI C_TGCA'G Cuts at: 830 3456 3884 830 Size: 2626 428 3478 Fragments arranged by size: 3478 2626 428 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6532 PvuII CAG'CTG Cuts at: 3304 3304 Size: 6532 SacI G_AGCT'C Cuts at: 3140 3140 Size: 6532 SalI G'TCGA_C Cuts at: 1936 3874 1936 Size: 1938 4594 SapI GCTCTTCn'nnn_ Cuts at: 3003 3003 Size: 6532 SbfI CC_TGCA'GG Cuts at: 3884 3884 Size: 6532 ScaI AGT'ACT Cuts at: 4780 6492 4780 Size: 1712 4820 SfcI C'TryA_G Cuts at: 826 1045 2653 3452 3880 5015 5696 5887 826 Size: 219 1608 799 428 1135 681 191 1471 Fragments arranged by size: 1608 1471 1135 799 681 428 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6532 SmaI CCC'GGG Cuts at: 1872 3859 1872 Size: 1987 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2846 4636 5537 5799 820 Size: 197 926 903 1790 901 262 1553 Fragments arranged by size: 1790 1553 926 903 901 262 197 SnaBI TAC'GTA Cuts at: 493 4323 493 Size: 3830 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6532 SphI G_CATG'C Cuts at: 1132 3890 1132 Size: 2758 3774 SspI AAT'ATT Cuts at: 5 52 2099 2308 4456 5 Size: 47 2047 209 2148 2081 Fragments arranged by size: 2148 2081 2047 209 47 StuI AGG'CCT Cuts at: 1517 3479 3755 1517 Size: 1962 276 4294 Fragments arranged by size: 4294 1962 276 StyI C'CwwG_G Cuts at: 513 1065 2139 2530 2748 3480 3486 3531 3564 Size: 552 1074 391 218 732 6 45 33 Cuts at: 3564 3669 3971 513 Size: 105 302 3074 Fragments arranged by size: 3074 1074 732 552 391 302 218 105 45 33 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4559 4576 4729 4914 5359 325 Size: 1255 2979 17 153 185 445 1498 Fragments arranged by size: 2979 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2214 2921 4778 Size: 274 80 33 51 1443 237 707 1857 Cuts at: 4778 6490 96 Size: 1712 138 Fragments arranged by size: 1857 1712 1443 707 274 237 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6492 6524 6492 Size: 32 6500 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6532 XbaI T'CTAG_A Cuts at: 2353 3868 2353 Size: 1515 5017 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6532 XmaI C'CCGG_G Cuts at: 1870 3857 1870 Size: 1987 4545 XmnI GAAnn'nnTTC Cuts at: 4661 4661 Size: 6532 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4340 276 Size: 53 83 186 936 2806 2468 Fragments arranged by size: 2806 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI