Restriction map for
pFN21AB5432
(Linear) (Six-base) MAPSORT of: KIBB5432.seq Check: 1062 from: 1 to: 1827
KIBB5432.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1362 1827
Size: 1362 465
Acc65I G'GTAC_C
Cuts at: 0 265 1827
Size: 265 1562
AclI AA'CG_TT
Cuts at: 0 387 1827
Size: 387 1440
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 802 1087 1443 1628 1711 1789 1801
Size: 634 168 285 356 185 83 78 12
Cuts at: 1801 1827
Size: 26
Fragments arranged by size:
634 356 285 185 168 83 78 26
12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1206 1827
Size: 1206 621
AlwNI CAG_nnn'CTG
Cuts at: 0 321 1794 1827
Size: 321 1473 33
Fragments arranged by size:
1473 321 33
ApoI r'AATT_y
Cuts at: 0 282 291 344 1271 1827
Size: 282 9 53 927 556
Fragments arranged by size:
927 556 282 53 9
BanI G'GyrC_C
Cuts at: 0 265 1239 1827
Size: 265 974 588
Fragments arranged by size:
974 588 265
BanII G_rGCy'C
Cuts at: 0 1135 1558 1827
Size: 1135 423 269
Fragments arranged by size:
1135 423 269
BbsI GAAGACnn'nnnn_
Cuts at: 0 521 725 1699 1827
Size: 521 204 974 128
Fragments arranged by size:
974 521 204 128
BciVI GTATCCnnnnn_n'
Cuts at: 0 208 1827
Size: 208 1619
BglI GCCn_nnn'nGGC
Cuts at: 0 136 553 1756 1827
Size: 136 417 1203 71
Fragments arranged by size:
1203 417 136 71
BglII A'GATC_T
Cuts at: 0 240 959 1827
Size: 240 719 868
Fragments arranged by size:
868 719 240
Bme1580I G_kGCm'C
Cuts at: 0 1736 1827
Size: 1736 91
BmrI ACTGGGnnnn_n'
Cuts at: 0 1561 1827
Size: 1561 266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1189 1271 1349 1489 1827
Size: 1006 183 82 78 140 338
Fragments arranged by size:
1006 338 183 140 82 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1827
Size: 826 1001
BsaI GGTCTCn'nnnn_
Cuts at: 0 742 959 1827
Size: 742 217 868
Fragments arranged by size:
868 742 217
BsaBI GATnn'nnATC
Cuts at: 0 239 1601 1827
Size: 239 1362 226
Fragments arranged by size:
1362 239 226
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 839 1126 1274 1699 1827
Size: 839 287 148 425 128
Fragments arranged by size:
839 425 287 148 128
BseYI C'CCAG_C
Cuts at: 0 1716 1827
Size: 1716 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1212 1827
Size: 1212 615
BsiHKAI G_wGCw'C
Cuts at: 0 765 1135 1152 1827
Size: 765 370 17 675
Fragments arranged by size:
765 675 370 17
Bsp1286I G_dGCh'C
Cuts at: 0 765 1135 1152 1558 1736 1827
Size: 765 370 17 406 178 91
Fragments arranged by size:
765 406 370 178 91 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 761 1362 1827
Size: 761 601 465
Fragments arranged by size:
761 601 465
BsrDI GCAATG_nn'
Cuts at: 0 537 1519 1544 1827
Size: 537 982 25 283
Fragments arranged by size:
982 537 283 25
BsrFI r'CCGG_y
Cuts at: 0 568 1827
Size: 568 1259
BsrGI T'GTAC_A
Cuts at: 0 209 1827
Size: 209 1618
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1794 1827
Size: 1794 33
BstYI r'GATC_y
Cuts at: 0 240 412 959 1827
Size: 240 172 547 868
Fragments arranged by size:
868 547 240 172
Bsu36I CC'TnA_GG
Cuts at: 0 685 1084 1827
Size: 685 399 743
Fragments arranged by size:
743 685 399
BtgI C'CryG_G
Cuts at: 0 408 525 1827
Size: 408 117 1302
Fragments arranged by size:
1302 408 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 50 1827
Size: 50 1777
BtsI GCAGTG_nn'
Cuts at: 0 237 1514 1827
Size: 237 1277 313
Fragments arranged by size:
1277 313 237
DraI TTT'AAA
Cuts at: 0 297 1827
Size: 297 1530
DraIII CAC_nnn'GTG
Cuts at: 0 430 1827
Size: 430 1397
EarI CTCTTCn'nnn_
Cuts at: 0 56 96 998 1247 1676 1765 1827
Size: 56 40 902 249 429 89 62
Fragments arranged by size:
902 429 249 89 62 56 40
EcoICRI GAG'CTC
Cuts at: 0 1133 1827
Size: 1133 694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 802 1006 1087 1189 1271 1349 1443
Size: 634 168 204 81 102 82 78 94
Cuts at: 1443 1489 1628 1711 1789 1801 1827
Size: 46 139 83 78 12 26
Fragments arranged by size:
634 204 168 139 102 94 83 82
81 78 78 46 26 12
EcoO109I rG'GnC_Cy
Cuts at: 0 681 1088 1827
Size: 681 407 739
Fragments arranged by size:
739 681 407
EcoRI G'AATT_C
Cuts at: 0 344 1827
Size: 344 1483
EcoRV GAT'ATC
Cuts at: 0 657 1827
Size: 657 1170
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 85 1356 1425 1827
Size: 85 1271 69 402
Fragments arranged by size:
1271 402 85 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 644 676 1827
Size: 644 32 1151
Fragments arranged by size:
1151 644 32
HincII GTy'rAC
Cuts at: 0 170 1827
Size: 170 1657
HindIII A'AGCT_T
Cuts at: 0 1402 1827
Size: 1402 425
KpnI G_GTAC'C
Cuts at: 0 269 1827
Size: 269 1558
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1238 1827
Size: 1238 589
MslI CAynn'nnrTG
Cuts at: 0 530 1637 1827
Size: 530 1107 190
Fragments arranged by size:
1107 530 190
MspA1I CmG'CkG
Cuts at: 0 1299 1827
Size: 1299 528
NcoI C'CATG_G
Cuts at: 0 525 1827
Size: 525 1302
PfoI T'CCnGG_A
Cuts at: 0 1820 1827
Size: 1820 7
PpuMI rG'GwC_Cy
Cuts at: 0 681 1827
Size: 681 1146
PsiI TTA'TAA
Cuts at: 0 821 1827
Size: 821 1006
PstI C_TGCA'G
Cuts at: 0 1451 1827
Size: 1451 376
PvuII CAG'CTG
Cuts at: 0 1299 1827
Size: 1299 528
SacI G_AGCT'C
Cuts at: 0 1135 1827
Size: 1135 692
SapI GCTCTTCn'nnn_
Cuts at: 0 998 1827
Size: 998 829
SfcI C'TryA_G
Cuts at: 0 648 1447 1827
Size: 648 799 380
Fragments arranged by size:
799 648 380
SmlI C'TyrA_G
Cuts at: 0 841 1827
Size: 841 986
SspI AAT'ATT
Cuts at: 0 94 303 1827
Size: 94 209 1524
Fragments arranged by size:
1524 209 94
StuI AGG'CCT
Cuts at: 0 1474 1750 1827
Size: 1474 276 77
Fragments arranged by size:
1474 276 77
StyI C'CwwG_G
Cuts at: 0 134 525 743 1475 1481 1526 1559 1664
Size: 134 391 218 732 6 45 33 105
Cuts at: 1664 1827
Size: 163
Fragments arranged by size:
732 391 218 163 134 105 45 33
6
TatI w'GTAC_w
Cuts at: 0 209 916 1827
Size: 209 707 911
Fragments arranged by size:
911 707 209
XbaI T'CTAG_A
Cuts at: 0 348 1827
Size: 348 1479
Enzymes that do cut:
AarI Acc65I AclI AcuI AlfI AlwNI ApoI BanI
BanII BbsI BciVI BglI BglII Bme1580I BmrI BpmI
BpuEI BsaI BsaBI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstAPI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII EarI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HindIII KpnI MmeI
MslI MspA1I NcoI PfoI PpuMI PsiI PstI PvuII
SacI SapI SfcI SmlI SspI StuI StyI TatI
XbaI
Enzymes that do not cut:
AatII AccI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BlpI
BmgBI BmtI BplI Bpu10I BsaAI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII
BssSI BstBI BstEII BstXI BstZ17I ClaI CspCI DrdI
EaeI EagI EciI EcoNI FseI FspI FspAI HaeII
HpaI KasI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI TaqII TstI Tth111I XcmI XhoI
XmaI XmnI ZraI