(Circular) (Six-base) MAPSORT of: pFN21AB5383.seq Check: 524 from: 1 to: 6322 pFN21AB5383 6322 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4132 278 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3665 1937 Size: 1728 4594 Acc65I G'GTAC_C Cuts at: 3643 3643 Size: 6322 AclI AA'CG_TT Cuts at: 1563 4449 4822 1563 Size: 2886 373 3063 Fragments arranged by size: 3063 2886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2092 2360 4385 5769 6037 1632 Size: 460 268 2025 1384 268 1917 Fragments arranged by size: 2025 1917 1384 460 268 268 AfeI AGC'GCT Cuts at: 3212 3212 Size: 6322 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6125 AflIII A'CryG_T Cuts at: 1203 2454 5221 6198 1203 Size: 1251 2767 977 1327 Fragments arranged by size: 2767 1327 1251 977 AgeI A'CCGG_T Cuts at: 5153 5153 Size: 6322 AhdI GACnn_n'nnGTC Cuts at: 5051 5051 Size: 6322 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6322 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6322 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3513 3545 3513 Size: 32 6290 AlwNI CAG_nnn'CTG Cuts at: 3538 4065 5637 3538 Size: 527 1572 4223 Fragments arranged by size: 4223 1572 527 ApaLI G'TGCA_C Cuts at: 4379 5535 4379 Size: 1156 5166 ApoI r'AATT_y Cuts at: 1493 2707 2857 3005 3631 3857 4009 6118 1493 Size: 1214 150 148 626 226 152 2109 1697 Fragments arranged by size: 2109 1697 1214 626 226 152 150 148 AseI AT'TA_AT Cuts at: 160 1032 3483 4876 160 Size: 872 2451 1393 1606 Fragments arranged by size: 2451 1606 1393 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 AvaI C'yCGr_G Cuts at: 1870 1943 2009 3618 3647 1870 Size: 73 66 1609 29 4545 Fragments arranged by size: 4545 1609 73 66 29 BamHI G'GATC_C Cuts at: 3652 4019 3652 Size: 367 5955 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 2069 2318 3643 5098 Size: 325 217 627 233 49 249 1325 1455 Cuts at: 5098 618 Size: 1842 Fragments arranged by size: 1842 1455 1325 627 325 249 233 217 49 BanII G_rGCy'C Cuts at: 1391 3265 3641 1391 Size: 1874 376 4072 Fragments arranged by size: 4072 1874 376 BbeI G_GCGC'C Cuts at: 2073 2073 Size: 6322 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3003 928 Size: 971 1104 4247 Fragments arranged by size: 4247 1104 971 BbvCI CC'TCA_GC Cuts at: 3090 3090 Size: 6322 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4513 4547 1903 Size: 34 50 34 2492 34 3678 Fragments arranged by size: 3678 2492 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4214 5424 4214 Size: 1210 5112 BclI T'GATC_A Cuts at: 1548 1548 Size: 6322 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4933 136 Size: 107 122 71 631 605 3261 1525 Fragments arranged by size: 3261 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2614 6317 2614 Size: 3703 2619 BlpI GC'TnA_GC Cuts at: 3207 3739 3207 Size: 532 5790 Bme1580I G_kGCm'C Cuts at: 1790 4383 5539 1790 Size: 2593 1156 2573 Fragments arranged by size: 2593 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6322 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4053 5011 442 Size: 416 53 83 399 24 2636 958 1753 Fragments arranged by size: 2636 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3270 4982 1239 Size: 2031 1712 2579 Fragments arranged by size: 2579 2031 1712 Bpu10I CC'TnA_GC Cuts at: 3090 3090 Size: 6322 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2280 3110 4447 5312 5610 2280 Size: 830 1337 865 298 2992 Fragments arranged by size: 2992 1337 865 830 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4985 882 Size: 617 3486 2219 Fragments arranged by size: 3486 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4113 493 Size: 711 2909 2702 Fragments arranged by size: 2909 2702 711 BsaBI GATnn'nnATC Cuts at: 6316 6316 Size: 6322 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 4129 4511 5146 Size: 53 83 186 936 537 2059 382 635 Cuts at: 5146 275 Size: 1451 Fragments arranged by size: 2059 1451 936 635 537 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4755 5153 5427 5574 5985 4755 Size: 398 274 147 411 5092 Fragments arranged by size: 5092 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6097 Fragments arranged by size: 6097 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3107 1186 Size: 1921 4401 BseYI C'CCAG_C Cuts at: 2593 3070 5525 6021 2593 Size: 477 2455 496 2894 Fragments arranged by size: 2894 2455 496 477 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5749 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2059 3772 4533 5561 1540 Size: 275 186 58 1713 761 1028 2301 Fragments arranged by size: 2301 1713 1028 761 275 186 58 BsiHKAI G_wGCw'C Cuts at: 3286 3559 4383 4468 5539 3286 Size: 273 824 85 1071 4069 Fragments arranged by size: 4069 1071 824 273 85 BsmI GAATG_Cn' Cuts at: 2277 3588 3833 3926 2277 Size: 1311 245 93 4673 Fragments arranged by size: 4673 1311 245 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3265 3286 3559 3641 4383 4468 5539 Size: 399 1475 21 273 82 742 85 1071 Cuts at: 5539 1391 Size: 2174 Fragments arranged by size: 2174 1475 1071 742 399 273 85 82 21 BspEI T'CCGG_A Cuts at: 5985 5985 Size: 6322 BspHI T'CATG_A Cuts at: 3220 4211 5941 3220 Size: 991 1730 3601 Fragments arranged by size: 3601 1730 991 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3677 844 Size: 2833 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2031 4209 1394 Size: 429 208 2178 3507 Fragments arranged by size: 3507 2178 429 208 BsrDI GCAATG_nn' Cuts at: 65 1248 2337 4817 4991 65 Size: 1183 1089 2480 174 1396 Fragments arranged by size: 2480 1396 1183 1089 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 3410 3450 4966 5153 1700 Size: 110 241 1359 40 1516 187 2869 Fragments arranged by size: 2869 1516 1359 241 187 110 40 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6322 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6322 BssSI C'ACGA_G Cuts at: 4382 5394 4382 Size: 1012 5310 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6322 BstBI TT'CG_AA Cuts at: 5126 5126 Size: 6322 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6322 BstXI CCAn_nnnn'nTGG Cuts at: 2275 3514 2275 Size: 1239 5083 BstYI r'GATC_y Cuts at: 1972 2100 2614 3652 4019 4404 4421 5873 5959 Size: 128 514 1038 367 385 17 1452 86 Cuts at: 5959 5971 6317 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1038 514 385 367 346 128 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2059 513 Size: 552 994 4776 Fragments arranged by size: 4776 994 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5583 Fragments arranged by size: 5583 683 56 BtsI GCAGTG_nn' Cuts at: 2580 3838 4632 4652 2580 Size: 1258 794 20 4250 Fragments arranged by size: 4250 1258 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6322 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6287 DraI TTT'AAA Cuts at: 2856 3105 3626 2856 Size: 249 521 5552 Fragments arranged by size: 5552 521 249 DraIII CAC_nnn'GTG Cuts at: 3335 3335 Size: 6322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2455 5329 809 Size: 507 1139 2874 1802 Fragments arranged by size: 2874 1802 1139 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2056 3769 4658 Size: 54 1425 50 275 18 226 1713 889 Cuts at: 4658 6287 8 Size: 1629 43 Fragments arranged by size: 1713 1629 1425 889 275 226 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2056 3769 1537 Size: 275 244 1713 4090 Fragments arranged by size: 4090 1713 275 244 EarI CTCTTCn'nnn_ Cuts at: 1353 2832 3251 4252 1353 Size: 1479 419 1001 3423 Fragments arranged by size: 3423 1479 1001 419 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2110 4909 5283 5429 387 Size: 1064 138 521 2799 374 146 1280 Fragments arranged by size: 2799 1280 1064 521 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2092 2360 3270 4385 4982 5769 6037 Size: 393 460 268 910 1115 597 787 268 Cuts at: 6037 1239 Size: 1524 Fragments arranged by size: 1524 1115 910 787 597 460 393 268 268 EcoNI CCTnn'n_nnAGG Cuts at: 2429 2429 Size: 6322 EcoO109I rG'GnC_Cy Cuts at: 2422 2422 Size: 6322 EcoRI G'AATT_C Cuts at: 3005 3631 4009 3005 Size: 626 378 5318 Fragments arranged by size: 5318 626 378 FspI TGC'GCA Cuts at: 4828 4828 Size: 6322 HaeII r_GCGC'y Cuts at: 1678 2073 3214 5469 1678 Size: 395 1141 2255 2531 Fragments arranged by size: 2531 2255 1141 395 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2877 2909 4963 4995 5037 Size: 32 135 32 1305 32 2054 32 42 Cuts at: 5037 5069 1373 Size: 32 2626 Fragments arranged by size: 2626 2054 1305 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3666 3912 669 Size: 988 281 1728 246 3079 Fragments arranged by size: 3079 1728 988 281 246 HindIII A'AGCT_T Cuts at: 748 2369 2642 748 Size: 1621 273 4428 Fragments arranged by size: 4428 1621 273 HpaI GTT'AAC Cuts at: 3912 3912 Size: 6322 KasI G'GCGC_C Cuts at: 2069 2069 Size: 6322 KpnI G_GTAC'C Cuts at: 3647 3647 Size: 6322 MfeI C'AATT_G Cuts at: 2655 3021 3165 3921 2655 Size: 366 144 756 5056 Fragments arranged by size: 5056 756 366 144 MluI A'CGCG_T Cuts at: 6198 6198 Size: 6322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2417 3531 5436 5620 1305 Size: 1112 1114 1905 184 2007 Fragments arranged by size: 2007 1905 1114 1112 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4500 Fragments arranged by size: 4500 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4280 4639 4798 518 Size: 648 3114 359 159 2042 Fragments arranged by size: 3114 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2061 3738 4415 5563 5808 1606 Size: 455 1677 677 1148 245 2120 Fragments arranged by size: 2120 1677 1148 677 455 245 NaeI GCC'GGC Cuts at: 2053 3412 2053 Size: 1359 4963 NarI GG'CG_CC Cuts at: 2070 2070 Size: 6322 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5770 NdeI CA'TA_TG Cuts at: 387 387 Size: 6322 NgoMIV G'CCGG_C Cuts at: 2051 3410 2051 Size: 1359 4963 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6322 NotI GC'GGCC_GC Cuts at: 2056 3769 2056 Size: 1713 4609 NspI r_CATG'y Cuts at: 1132 1229 2458 3070 3458 3680 5225 1132 Size: 97 1229 612 388 222 1545 2229 Fragments arranged by size: 2229 1545 1229 612 388 222 97 PasI CC'CwG_GG Cuts at: 1348 3460 1348 Size: 2112 4210 PciI A'CATG_T Cuts at: 2454 5221 2454 Size: 2767 3555 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6322 PmeI GTTT'AAAC Cuts at: 3626 3626 Size: 6322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4391 4423 5904 5936 4391 Size: 32 1481 32 4777 Fragments arranged by size: 4777 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6322 PsiI TTA'TAA Cuts at: 3892 3892 Size: 6322 PspXI vC'TCGA_Gb Cuts at: 3618 3618 Size: 6322 PstI C_TGCA'G Cuts at: 830 3478 3674 830 Size: 2648 196 3478 Fragments arranged by size: 3478 2648 196 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6322 SacII CC_GC'GG Cuts at: 2062 2062 Size: 6322 SalI G'TCGA_C Cuts at: 1936 3664 1936 Size: 1728 4594 SbfI CC_TGCA'GG Cuts at: 3674 3674 Size: 6322 ScaI AGT'ACT Cuts at: 4570 6282 4570 Size: 1712 4610 SfcI C'TryA_G Cuts at: 826 1045 3429 3474 3670 4805 5486 5677 826 Size: 219 2384 45 196 1135 681 191 1471 Fragments arranged by size: 2384 1471 1135 681 219 196 191 45 SfoI GGC'GCC Cuts at: 2071 2071 Size: 6322 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6322 SmaI CCC'GGG Cuts at: 1872 3649 1872 Size: 1777 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2295 3125 3618 4426 5327 5589 Size: 197 926 352 830 493 808 901 262 Cuts at: 5589 820 Size: 1553 Fragments arranged by size: 1553 926 901 830 808 493 352 262 197 SnaBI TAC'GTA Cuts at: 493 4113 493 Size: 3620 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6322 SphI G_CATG'C Cuts at: 1132 3458 3680 1132 Size: 2326 222 3774 Fragments arranged by size: 3774 2326 222 SspI AAT'ATT Cuts at: 5 52 3320 4246 5 Size: 47 3268 926 2081 Fragments arranged by size: 3268 2081 926 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6322 StyI C'CwwG_G Cuts at: 513 1065 2158 3761 513 Size: 552 1093 1603 3074 Fragments arranged by size: 3074 1603 1093 552 SwaI ATTT'AAAT Cuts at: 3105 3105 Size: 6322 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2708 2838 4349 4366 4519 4704 5149 Size: 1255 1128 130 1511 17 153 185 445 Cuts at: 5149 325 Size: 1498 Fragments arranged by size: 1511 1498 1255 1128 445 185 153 130 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4568 6280 96 Size: 274 80 33 51 1443 2591 1712 138 Fragments arranged by size: 2591 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6282 6314 6282 Size: 32 6290 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6322 XbaI T'CTAG_A Cuts at: 3658 3658 Size: 6322 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2275 2779 2275 Size: 504 5818 XhoI C'TCGA_G Cuts at: 1943 3618 1943 Size: 1675 4647 XmaI C'CCGG_G Cuts at: 1870 3647 1870 Size: 1777 4545 XmnI GAAnn'nnTTC Cuts at: 3579 4451 3579 Size: 872 5450 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4130 276 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI