Restriction map for
pFN21AB5383
(Linear) (Six-base) MAPSORT of: KIBB5383.seq Check: 8229 from: 1 to: 1617
KIBB5383.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 355 1617
Size: 87 268 1262
Fragments arranged by size:
1262 268 87
AfeI AGC'GCT
Cuts at: 0 1207 1617
Size: 1207 410
AflIII A'CryG_T
Cuts at: 0 449 1617
Size: 449 1168
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1508 1540 1617
Size: 1508 32 77
Fragments arranged by size:
1508 77 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1533 1617
Size: 1533 84
ApoI r'AATT_y
Cuts at: 0 702 852 1000 1617
Size: 702 150 148 617
Fragments arranged by size:
702 617 150 148
AseI AT'TA_AT
Cuts at: 0 1478 1617
Size: 1478 139
AvaI C'yCGr_G
Cuts at: 0 4 1617
Size: 4 1613
BanI G'GyrC_C
Cuts at: 0 15 64 313 1617
Size: 15 49 249 1304
Fragments arranged by size:
1304 249 49 15
BanII G_rGCy'C
Cuts at: 0 1260 1617
Size: 1260 357
BbeI G_GCGC'C
Cuts at: 0 68 1617
Size: 68 1549
BbsI GAAGACnn'nnnn_
Cuts at: 0 998 1617
Size: 998 619
BbvCI CC'TCA_GC
Cuts at: 0 1085 1617
Size: 1085 532
BglII A'GATC_T
Cuts at: 0 609 1617
Size: 609 1008
BlpI GC'TnA_GC
Cuts at: 0 1202 1617
Size: 1202 415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1265 1617
Size: 1265 352
Bpu10I CC'TnA_GC
Cuts at: 0 1085 1617
Size: 1085 532
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1105 1617
Size: 275 830 512
Fragments arranged by size:
830 512 275
BsaHI Gr'CG_yC
Cuts at: 0 65 1617
Size: 65 1552
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1102 1617
Size: 1102 515
BseYI C'CCAG_C
Cuts at: 0 588 1065 1617
Size: 588 477 552
Fragments arranged by size:
588 552 477
BsiEI CG_ry'CG
Cuts at: 0 54 1617
Size: 54 1563
BsiHKAI G_wGCw'C
Cuts at: 0 1281 1554 1617
Size: 1281 273 63
Fragments arranged by size:
1281 273 63
BsmI GAATG_Cn'
Cuts at: 0 272 1583 1617
Size: 272 1311 34
Fragments arranged by size:
1311 272 34
Bsp1286I G_dGCh'C
Cuts at: 0 1260 1281 1554 1617
Size: 1260 21 273 63
Fragments arranged by size:
1260 273 63 21
BspHI T'CATG_A
Cuts at: 0 1215 1617
Size: 1215 402
BsrBI CCG'CTC
Cuts at: 0 26 1617
Size: 26 1591
BsrDI GCAATG_nn'
Cuts at: 0 332 1617
Size: 332 1285
BsrFI r'CCGG_y
Cuts at: 0 46 1405 1445 1617
Size: 46 1359 40 172
Fragments arranged by size:
1359 172 46 40
BstXI CCAn_nnnn'nTGG
Cuts at: 0 270 1509 1617
Size: 270 1239 108
Fragments arranged by size:
1239 270 108
BstYI r'GATC_y
Cuts at: 0 95 609 1617
Size: 95 514 1008
Fragments arranged by size:
1008 514 95
BtgI C'CryG_G
Cuts at: 0 54 1617
Size: 54 1563
BtsI GCAGTG_nn'
Cuts at: 0 575 1617
Size: 575 1042
DraI TTT'AAA
Cuts at: 0 851 1100 1617
Size: 851 249 517
Fragments arranged by size:
851 517 249
DraIII CAC_nnn'GTG
Cuts at: 0 1330 1617
Size: 1330 287
DrdI GACnn_nn'nnGTC
Cuts at: 0 450 1617
Size: 450 1167
EaeI y'GGCC_r
Cuts at: 0 51 1617
Size: 51 1566
EagI C'GGCC_G
Cuts at: 0 51 1617
Size: 51 1566
EarI CTCTTCn'nnn_
Cuts at: 0 827 1246 1617
Size: 827 419 371
Fragments arranged by size:
827 419 371
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 105 1617
Size: 105 1512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 355 1265 1617
Size: 87 268 910 352
Fragments arranged by size:
910 352 268 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 424 1617
Size: 424 1193
EcoO109I rG'GnC_Cy
Cuts at: 0 417 1617
Size: 417 1200
EcoRI G'AATT_C
Cuts at: 0 1000 1617
Size: 1000 617
HaeII r_GCGC'y
Cuts at: 0 68 1209 1617
Size: 68 1141 408
Fragments arranged by size:
1141 408 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 872 904 1617
Size: 872 32 713
Fragments arranged by size:
872 713 32
HindIII A'AGCT_T
Cuts at: 0 364 637 1617
Size: 364 273 980
Fragments arranged by size:
980 364 273
KasI G'GCGC_C
Cuts at: 0 64 1617
Size: 64 1553
MfeI C'AATT_G
Cuts at: 0 650 1016 1160 1617
Size: 650 366 144 457
Fragments arranged by size:
650 457 366 144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1526 1617
Size: 412 1114 91
Fragments arranged by size:
1114 412 91
MspA1I CmG'CkG
Cuts at: 0 56 1617
Size: 56 1561
NaeI GCC'GGC
Cuts at: 0 48 1407 1617
Size: 48 1359 210
Fragments arranged by size:
1359 210 48
NarI GG'CG_CC
Cuts at: 0 65 1617
Size: 65 1552
NgoMIV G'CCGG_C
Cuts at: 0 46 1405 1617
Size: 46 1359 212
Fragments arranged by size:
1359 212 46
NotI GC'GGCC_GC
Cuts at: 0 51 1617
Size: 51 1566
NspI r_CATG'y
Cuts at: 0 453 1065 1453 1617
Size: 453 612 388 164
Fragments arranged by size:
612 453 388 164
PasI CC'CwG_GG
Cuts at: 0 1455 1617
Size: 1455 162
PciI A'CATG_T
Cuts at: 0 449 1617
Size: 449 1168
PstI C_TGCA'G
Cuts at: 0 1473 1617
Size: 1473 144
SacII CC_GC'GG
Cuts at: 0 57 1617
Size: 57 1560
SfcI C'TryA_G
Cuts at: 0 1424 1469 1617
Size: 1424 45 148
Fragments arranged by size:
1424 148 45
SfoI GGC'GCC
Cuts at: 0 66 1617
Size: 66 1551
SmlI C'TyrA_G
Cuts at: 0 290 1120 1617
Size: 290 830 497
Fragments arranged by size:
830 497 290
SphI G_CATG'C
Cuts at: 0 1453 1617
Size: 1453 164
SspI AAT'ATT
Cuts at: 0 1315 1617
Size: 1315 302
StyI C'CwwG_G
Cuts at: 0 153 1617
Size: 153 1464
SwaI ATTT'AAAT
Cuts at: 0 1100 1617
Size: 1100 517
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 703 833 1617
Size: 703 130 784
Fragments arranged by size:
784 703 130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 270 774 1617
Size: 270 504 843
Fragments arranged by size:
843 504 270
XmnI GAAnn'nnTTC
Cuts at: 0 1574 1617
Size: 1574 43
Enzymes that do cut:
AcuI AfeI AflIII AloI AlwNI ApoI AseI AvaI
BanI BanII BbeI BbsI BbvCI BglII BlpI BpmI
Bpu10I BpuEI BsaHI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BsrBI BsrDI BsrFI BstXI BstYI BtgI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HindIII KasI
MfeI MmeI MspA1I NaeI NarI NgoMIV NotI NspI
PasI PciI PstI SacII SfcI SfoI SmlI SphI
SspI StyI SwaI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglI Bme1580I BmgBI
BmrI BmtI BplI BsaI BsaAI BsaBI BsaWI BsaXI
BsgI BsiWI BsmBI BspEI BspMI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
EcoICRI EcoRV FalI FseI FspI FspAI HincII HpaI
KpnI MluI MscI MslI NcoI NdeI NheI NruI
NsiI PacI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI TatI
TstI Tth111I XbaI XhoI XmaI ZraI