Restriction map for
pFN21AB5363
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HaeIII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 142 GTCTAC GT^[AC][GT]AC
Asp700I 124 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 327 GGACC G^G[AT]CC
BbrPI 402 CACGTG CAC^GTG
BbvI 433 GCAGC GCAGC(8/12)
BmyI 405 GTGCCC G[AGT]GC[ACT]^C
BsaAI 402 CACGTG [CT]AC^GT[GA]
BsiEI 63 CGACCG CG[GA][CT]^CG
BsiYI 21 CCCCACCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 21 CCCCACCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 405 GTGCCC G[AGT]GC[ACT]^C
BssSI 49 CACGAG CACGAG(-5/-1)
Cfr13I 327 GGACC G^G[ACGT]CC
DraII 326 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 326 GGGACCC [GA]G^G[ACGT]CC[CT]
HphI 363 TCACC GGTGA(8/7)
MaeII 403 ACGT A^CGT
MspA1I 181 CAGCTG C[AC]G^C[GT]G
NspI 350 GCATGC [GA]CATG^[CT]
PmaCI 402 CACGTG CAC^GTG
PmlI 402 CACGTG CAC^GTG
PpuMI 326 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 181 CAGCTG CAG^CTG
Sau96I 327 GGACC G^G[ACGT]CC
SfaNI 430 GATGC GCATC(5/9)
SphI 350 GCATGC GCATG^C
TaqI 336 TCGA T^CGA
TseI 433 GCAGC G^C[AT]GC
TspRI 437 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 336 TCGA T^CGA
XmnI 124 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BcnI 66 CCGGG CC^[GC]GG
288 CCGGG
BsaJI 26 CCTCGG C^C[ACGT][ACGT]GG
258 CCCTGG
BsmAI 454 GAGAC GTCTC(1/5)
54 GTCTC
BsmFI 85 GGGAC GGGAC(10/14)
326 GGGAC
BstF5I 446 CATCC GGATG(2/0)
30 GGATG
BstNI 259 CCTGG CC^[AT]GG
366 CCTGG
BstYI 284 AGATCC [GA]^GATC[CT]
394 AGATCC
DdeI 56 CTCAG C^T[ACGT]AG
179 CTCAG
EarI 190 GAAGAG CTCTTC(1/4)
263 GAAGAG
EcoRII 259 CCTGG ^CC[AT]GG
366 CCTGG
FokI 446 CATCC GGATG(9/13)
30 GGATG
HinfI 52 GAGTC G^A[ACGT]TC
165 GACTC
Ksp632I 190 GAAGAG CTCTTC(1/4)
263 GAAGAG
MboII 190 GAAGA GAAGA(8/7)
263 GAAGA
MflI 284 AGATCC [GA]^GATC[CT]
394 AGATCC
MvaI 259 CCTGG CC^[AT]GG
366 CCTGG
NciI 66 CCGGG CC^[GC]GG
288 CCGGG
NheI 70 GCTAGC G^CTAGC
171 GCTAGC
NlaIII 351 CATG CATG^
417 CATG
PleI 165 GACTC GAGTC(4/5)
52 GAGTC
Tsp509I 201 AATT ^AATT
333 AATT
XhoII 284 AGATCC [GA]^GATC[CT]
394 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AluI AlwI BfaI BsrI
BssKI Cac8I DpnII Fnu4HI HapII
HpaII ItaI MaeI MboI MnlI
MspI MwoI NdeII NlaIV Sau3AI
ScrFI