Restriction map for
pFN21AB5341
(Linear) (Six-base) MAPSORT of: KIBB5341.seq Check: 7061 from: 1 to: 1038
KIBB5341.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 718 1038
Size: 296 422 320
Fragments arranged by size:
422 320 296
AlwNI CAG_nnn'CTG
Cuts at: 0 881 1038
Size: 881 157
ApaI G_GGCC'C
Cuts at: 0 575 1038
Size: 575 463
AvaI C'yCGr_G
Cuts at: 0 110 1038
Size: 110 928
BanII G_rGCy'C
Cuts at: 0 234 297 575 1038
Size: 234 63 278 463
Fragments arranged by size:
463 278 234 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 627 1038
Size: 627 411
BciVI GTATCCnnnnn_n'
Cuts at: 0 481 1038
Size: 481 557
BglII A'GATC_T
Cuts at: 0 431 1038
Size: 431 607
Bme1580I G_kGCm'C
Cuts at: 0 575 1038
Size: 575 463
BmrI ACTGGGnnnn_n'
Cuts at: 0 260 764 847 895 1038
Size: 260 504 83 48 143
Fragments arranged by size:
504 260 143 83 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 200 387 679 690 793 1038
Size: 103 97 187 292 11 103 245
Fragments arranged by size:
292 245 187 103 103 97 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 871 901 1038
Size: 871 30 137
Fragments arranged by size:
871 137 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 52 55 541 959 1038
Size: 28 24 3 486 418 79
Fragments arranged by size:
486 418 79 28 24 3
BsiHKAI G_wGCw'C
Cuts at: 0 234 297 737 1038
Size: 234 63 440 301
Fragments arranged by size:
440 301 234 63
Bsp1286I G_dGCh'C
Cuts at: 0 234 297 575 737 1038
Size: 234 63 278 162 301
Fragments arranged by size:
301 278 234 162 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 863 1038
Size: 863 175
BsrFI r'CCGG_y
Cuts at: 0 59 1038
Size: 59 979
BstAPI GCAn_nnn'nTGC
Cuts at: 0 691 1038
Size: 691 347
BstYI r'GATC_y
Cuts at: 0 65 222 431 765 1038
Size: 65 157 209 334 273
Fragments arranged by size:
334 273 209 157 65
Bsu36I CC'TnA_GG
Cuts at: 0 863 1038
Size: 863 175
BtgI C'CryG_G
Cuts at: 0 311 483 1038
Size: 311 172 555
Fragments arranged by size:
555 311 172
BtsI GCAGTG_nn'
Cuts at: 0 681 1038
Size: 681 357
EaeI y'GGCC_r
Cuts at: 0 261 1038
Size: 261 777
EarI CTCTTCn'nnn_
Cuts at: 0 756 1038
Size: 756 282
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 58 1038
Size: 58 980
EcoICRI GAG'CTC
Cuts at: 0 232 295 1038
Size: 232 63 743
Fragments arranged by size:
743 232 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 200 296 387 679 690 718 793
Size: 103 97 96 91 292 11 28 75
Cuts at: 793 1038
Size: 245
Fragments arranged by size:
292 245 103 97 96 91 75 28
11
EcoO109I rG'GnC_Cy
Cuts at: 0 948 963 1038
Size: 948 15 75
Fragments arranged by size:
948 75 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 651 1038
Size: 651 387
HincII GTy'rAC
Cuts at: 0 426 1038
Size: 426 612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 897 1038
Size: 737 160 141
Fragments arranged by size:
737 160 141
MspA1I CmG'CkG
Cuts at: 0 960 1038
Size: 960 78
NcoI C'CATG_G
Cuts at: 0 311 483 1038
Size: 311 172 555
Fragments arranged by size:
555 311 172
NdeI CA'TA_TG
Cuts at: 0 783 1038
Size: 783 255
PfoI T'CCnGG_A
Cuts at: 0 225 1038
Size: 225 813
PpuMI rG'GwC_Cy
Cuts at: 0 963 1038
Size: 963 75
PspOMI G'GGCC_C
Cuts at: 0 571 1038
Size: 571 467
SacI G_AGCT'C
Cuts at: 0 234 297 1038
Size: 234 63 741
Fragments arranged by size:
741 234 63
SanDI GG'GwC_CC
Cuts at: 0 963 1038
Size: 963 75
StyI C'CwwG_G
Cuts at: 0 311 483 555 1026 1038
Size: 311 172 72 471 12
Fragments arranged by size:
471 311 172 72 12
Tth111I GACn'n_nGTC
Cuts at: 0 871 1038
Size: 871 167
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanII BbsI BciVI BglII
Bme1580I BmrI BpmI BsaXI BseRI BsiHKAI Bsp1286I BspMI
BsrFI BstAPI BstYI Bsu36I BtgI BtsI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I FalI HincII MmeI MspA1I
NcoI NdeI PfoI PpuMI PspOMI SacI SanDI StyI
Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BclI BfrBI BglI BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HindIII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI XbaI XcmI XhoI
XmaI XmnI ZraI