Restriction map for pFN21AB5302
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BciVI       BclI        BcnI        BglI        BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     Van91I      XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          494  GTCTAC               GT^[AC][GT]AC
AfaI          908  GTAC                 GT^AC
AflII         307  CTTAAG               C^TTAAG
AflIII        894  ACATGT               A^C[GA][CT]GT
AlwNI         152  CAGTTACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I         9  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          638  CTCGAG               C^[CT]CG[GA]G
BanI          749  GGTGCC               G^G[CT][GA]CC
BanII         716  GAGCTC               G[GA]GC[CT]^C
BcgI          135  GCAAGAGGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          307  CTTAAG               C^TTAAG
BsaI          505  GGTCTC               GGTCTC(1/5)
BsaWI         758  ACCGGA               [AT]^CCGG[AT]
BsmFI          75  GTCCC                GGGAC(10/14)
BsoBI         638  CTCGAG               C^[CT]CG[GA]G
BspLU11I      894  ACATGT               A^CATGT
BssKI          78  CCAGG                ^CC[ACGT]GG
BstEII        808  GGTAACC              G^GT[ACGT]ACC
BstF5I        477  GGATG                GGATG(2/0)
BstNI          78  CCAGG                CC^[AT]GG
BstPI         808  GGTAACC              G^GT[ACGT]ACC
BstXI          20  CCAAGGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI          674  CTGAG                C^T[ACGT]AG
DsaI          615  CCGTGG               C^C[GA][CT]GG
EcoNI         877  CCTCGTAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       808  GGTAACC              G^GT[ACGT]ACC
EcoRII         78  CCAGG                ^CC[AT]GG
FokI          477  GGATG                GGATG(9/13)
HaeIII        697  GGCC                 GG^CC
HapII         759  CCGG                 C^CGG
HincII        259  GTTGAC               GT[CT]^[GA]AC
HindII        259  GTTGAC               GT[CT]^[GA]AC
HpaII         759  CCGG                 C^CGG
MspI          759  CCGG                 C^CGG
MvaI           78  CCAGG                CC^[AT]GG
NspI          894  ACATGT               [GA]CATG^[CT]
PaeR7I        638  CTCGAG               C^TCGAG
PleI           73  GAGTC                GAGTC(4/5)
RsaI          908  GTAC                 GT^AC
SacI          716  GAGCTC               GAGCT^C
ScrFI          78  CCAGG                CC^[ACGT]GG
SfaNI         329  GCATC                GCATC(5/9)
SfcI          518  CTATAG               C^T[GA][CT]AG
SspI          431  AATATT               AAT^ATT
Tsp45I        587  GTGAC                ^GT[GC]AC
XbaI          712  TCTAGA               T^CTAGA
XhoI          638  CTCGAG               C^TCGAG
XmnI            9  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AvaII          30  GGACC                G^G[AT]CC
              577  GGTCC               
BfaI          713  CTAG                 C^TAG
              727  CTAG                
BglII         441  AGATCT               A^GATCT
              485  AGATCT              
BseRI         635  CTCCTC               GAGGAG(10/8)
              641  GAGGAG              
BsmAI         676  GAGAC                GTCTC(1/5)
              506  GTCTC               
BspHI         186  TCATGA               T^CATGA
              351  TCATGA              
Cac8I          59  GCAAGC               GC[ACGT]^[ACGT]GC
              887  GCGAGC              
Cfr13I         30  GGACC                G^G[ACGT]CC
              577  GGTCC               
EcoT14I        20  CCAAGG               C^C[AT][AT]GG
              470  CCTTGG              
MaeI          713  CTAG                 C^TAG
              727  CTAG                
MaeII         131  ACGT                 A^CGT
              703  ACGT                
MslI          688  CATGCCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              702  CACGTTGGTG          
MspA1I         46  CAGCTG               C[AC]G^C[GT]G
               64  CAGCTG              
NlaIV         576  GGGTCC               GG[ACGT]^[ACGT]CC
              749  GGTGCC              
PvuII          46  CAGCTG               CAG^CTG
               64  CAGCTG              
RcaI          186  TCATGA               T^CATGA
              351  TCATGA              
Sau96I         30  GGACC                G^G[ACGT]CC
              577  GGTCC               
SmlI          307  CTTAAG               C^T[CT][GA]AG
              638  CTCGAG              
StyI           20  CCAAGG               C^C[AT][AT]GG
              470  CCTTGG              
TfiI          124  GAATC                G^A[AT]TC
              348  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AluI        AlwI        AspHI       BbvI        BmyI        
BsaJI       BsiHKAI     BsiYI       BslI        Bsp1286I    
BsrI        BstYI       DpnII       Fnu4HI      HinfI       
HphI        ItaI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        NdeII       NlaIII      
Sau3AI      TaqI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XhoII       
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