(Circular) (Six-base) MAPSORT of: pFN21AB5274.seq Check: 9416 from: 1 to: 5752 pFN21AB5274 5752 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 278 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2208 3095 1937 Size: 271 887 4594 Fragments arranged by size: 4594 887 271 Acc65I G'GTAC_C Cuts at: 3073 3073 Size: 5752 AclI AA'CG_TT Cuts at: 1563 3879 4252 1563 Size: 2316 373 3063 Fragments arranged by size: 3063 2316 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2547 3815 5199 5467 1632 Size: 915 1268 1384 268 1917 Fragments arranged by size: 1917 1384 1268 915 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5555 AflIII A'CryG_T Cuts at: 1203 4651 5628 1203 Size: 3448 977 1327 Fragments arranged by size: 3448 1327 977 AgeI A'CCGG_T Cuts at: 4583 4583 Size: 5752 AhdI GACnn_n'nnGTC Cuts at: 4481 4481 Size: 5752 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5752 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5752 AlwNI CAG_nnn'CTG Cuts at: 2525 3495 5067 2525 Size: 970 1572 3210 Fragments arranged by size: 3210 1572 970 ApaLI G'TGCA_C Cuts at: 3809 4965 3809 Size: 1156 4596 ApoI r'AATT_y Cuts at: 1493 2844 3061 3287 3439 5548 1493 Size: 1351 217 226 152 2109 1697 Fragments arranged by size: 2109 1697 1351 226 217 152 AseI AT'TA_AT Cuts at: 160 1032 4306 160 Size: 872 3274 1606 Fragments arranged by size: 3274 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 AvaI C'yCGr_G Cuts at: 1870 1943 3077 1870 Size: 73 1134 4545 Fragments arranged by size: 4545 1134 73 BamHI G'GATC_C Cuts at: 3082 3449 3082 Size: 367 5385 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3073 4528 618 Size: 325 217 627 1286 1455 1842 Fragments arranged by size: 1842 1455 1286 627 325 217 BanII G_rGCy'C Cuts at: 1391 2979 3071 1391 Size: 1588 92 4072 Fragments arranged by size: 4072 1588 92 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2459 2997 928 Size: 971 560 538 3683 Fragments arranged by size: 3683 971 560 538 BbvCI CC'TCA_GC Cuts at: 2681 2681 Size: 5752 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3943 3977 1903 Size: 34 2006 34 3678 Fragments arranged by size: 3678 2006 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2302 3644 4854 2302 Size: 1342 1210 3200 Fragments arranged by size: 3200 1342 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5752 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4363 136 Size: 107 122 71 631 605 2691 1525 Fragments arranged by size: 2691 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2758 5747 2758 Size: 2989 2763 BlpI GC'TnA_GC Cuts at: 3169 3169 Size: 5752 Bme1580I G_kGCm'C Cuts at: 1790 3813 4969 1790 Size: 2023 1156 2573 Fragments arranged by size: 2573 2023 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3483 4441 442 Size: 416 53 83 399 24 2066 958 1753 Fragments arranged by size: 2066 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2343 1056 Size: 1287 4465 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2052 2060 2466 2592 2783 4412 1239 Size: 813 8 406 126 191 1629 2579 Fragments arranged by size: 2579 1629 813 406 191 126 8 Bpu10I CC'TnA_GC Cuts at: 2681 2681 Size: 5752 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2105 2930 3877 4742 5040 2105 Size: 825 947 865 298 2817 Fragments arranged by size: 2817 947 865 825 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2702 2879 4415 882 Size: 617 1203 177 1536 2219 Fragments arranged by size: 2219 1536 1203 617 177 BsaAI yAC'GTr Cuts at: 493 1204 3543 493 Size: 711 2339 2702 Fragments arranged by size: 2702 2339 711 BsaBI GATnn'nnATC Cuts at: 2307 2313 5746 2307 Size: 6 3433 2313 Fragments arranged by size: 3433 2313 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2158 3559 3941 4576 Size: 53 83 186 936 625 1401 382 635 Cuts at: 4576 275 Size: 1451 Fragments arranged by size: 1451 1401 936 635 625 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2448 4185 4583 4857 5004 5415 2448 Size: 1737 398 274 147 411 2785 Fragments arranged by size: 2785 1737 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2033 2063 1178 Size: 30 165 30 630 30 4867 Fragments arranged by size: 4867 630 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2019 2342 3041 1186 Size: 833 323 699 3897 Fragments arranged by size: 3897 833 699 323 BseYI C'CCAG_C Cuts at: 2712 4955 5451 2712 Size: 2243 496 3013 Fragments arranged by size: 3013 2243 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5179 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3202 3963 4991 1540 Size: 275 186 1201 761 1028 2301 Fragments arranged by size: 2301 1201 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2979 3813 3898 4969 2979 Size: 834 85 1071 3762 Fragments arranged by size: 3762 1071 834 85 BsmI GAATG_Cn' Cuts at: 3263 3356 3263 Size: 93 5659 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2979 3071 3813 3898 4969 1391 Size: 399 1189 92 742 85 1071 2174 Fragments arranged by size: 2174 1189 1071 742 399 92 85 BspEI T'CCGG_A Cuts at: 2448 5415 2448 Size: 2967 2785 BspHI T'CATG_A Cuts at: 2922 3641 5371 2922 Size: 719 1730 3303 Fragments arranged by size: 3303 1730 719 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2220 3107 844 Size: 1376 887 3489 Fragments arranged by size: 3489 1376 887 BsrBI CCG'CTC Cuts at: 1394 1823 3639 1394 Size: 429 1816 3507 Fragments arranged by size: 3507 1816 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4247 4421 65 Size: 1183 2999 174 1396 Fragments arranged by size: 2999 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4396 4583 1700 Size: 110 2586 187 2869 Fragments arranged by size: 2869 2586 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5752 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5752 BssSI C'ACGA_G Cuts at: 3812 4824 3812 Size: 1012 4740 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5752 BstBI TT'CG_AA Cuts at: 4556 4556 Size: 5752 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5752 BstXI CCAn_nnnn'nTGG Cuts at: 2833 2833 Size: 5752 BstYI r'GATC_y Cuts at: 1972 2758 2914 3082 3449 3834 3851 5303 5389 Size: 786 156 168 367 385 17 1452 86 Cuts at: 5389 5401 5747 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 786 385 367 346 168 156 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2404 513 Size: 552 1339 3861 Fragments arranged by size: 3861 1339 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5013 Fragments arranged by size: 5013 683 56 BtsI GCAGTG_nn' Cuts at: 2698 3268 4062 4082 2698 Size: 570 794 20 4368 Fragments arranged by size: 4368 794 570 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5717 DraI TTT'AAA Cuts at: 3056 3056 Size: 5752 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4759 809 Size: 507 3443 1802 Fragments arranged by size: 3443 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3199 4088 5717 Size: 54 1425 50 275 18 1369 889 1629 Cuts at: 5717 8 Size: 43 Fragments arranged by size: 1629 1425 1369 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3199 1537 Size: 275 1387 4090 Fragments arranged by size: 4090 1387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2432 2608 3682 1353 Size: 1079 176 1074 3423 Fragments arranged by size: 3423 1079 1074 176 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4339 4713 4859 387 Size: 1064 138 2750 374 146 1280 Fragments arranged by size: 2750 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2977 2977 Size: 5752 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2052 2060 2466 2547 2592 2783 3815 Size: 393 420 8 406 81 45 191 1032 Cuts at: 3815 4412 5199 5467 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1032 787 597 420 406 393 268 191 81 45 8 EcoNI CCTnn'n_nnAGG Cuts at: 3047 3047 Size: 5752 EcoRI G'AATT_C Cuts at: 2844 3061 3439 2844 Size: 217 378 5157 Fragments arranged by size: 5157 378 217 EcoRV GAT'ATC Cuts at: 2275 2599 2275 Size: 324 5428 FspI TGC'GCA Cuts at: 4258 4258 Size: 5752 HaeII r_GCGC'y Cuts at: 1678 4899 1678 Size: 3221 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2701 2733 3020 3052 4393 Size: 32 135 32 1129 32 287 32 1341 Cuts at: 4393 4425 4467 4499 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1341 1129 287 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2116 3096 3342 669 Size: 988 281 178 980 246 3079 Fragments arranged by size: 3079 988 980 281 246 178 HindIII A'AGCT_T Cuts at: 748 2230 748 Size: 1482 4270 HpaI GTT'AAC Cuts at: 3342 3342 Size: 5752 KpnI G_GTAC'C Cuts at: 3077 3077 Size: 5752 MfeI C'AATT_G Cuts at: 2136 3351 2136 Size: 1215 4537 MluI A'CGCG_T Cuts at: 5628 5628 Size: 5752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4866 5050 1305 Size: 3561 184 2007 Fragments arranged by size: 3561 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3930 Fragments arranged by size: 3930 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2151 2721 2927 3710 4069 4228 518 Size: 648 985 570 206 783 359 159 2042 Fragments arranged by size: 2042 985 783 648 570 359 206 159 MspA1I CmG'CkG Cuts at: 1606 2270 2833 3168 3845 4993 5238 1606 Size: 664 563 335 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 664 563 335 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5200 NdeI CA'TA_TG Cuts at: 387 387 Size: 5752 NheI G'CTAG_C Cuts at: 1052 2339 1052 Size: 1287 4465 NotI GC'GGCC_GC Cuts at: 3199 3199 Size: 5752 NspI r_CATG'y Cuts at: 1132 1229 3110 4655 1132 Size: 97 1881 1545 2229 Fragments arranged by size: 2229 1881 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5752 PciI A'CATG_T Cuts at: 4651 4651 Size: 5752 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5752 PfoI T'CCnGG_A Cuts at: 2608 2608 Size: 5752 PmeI GTTT'AAAC Cuts at: 3056 3056 Size: 5752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3821 3853 5334 5366 3821 Size: 32 1481 32 4207 Fragments arranged by size: 4207 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5752 PsiI TTA'TAA Cuts at: 3322 3322 Size: 5752 PstI C_TGCA'G Cuts at: 830 3104 830 Size: 2274 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5752 PvuII CAG'CTG Cuts at: 2270 2833 2270 Size: 563 5189 SacI G_AGCT'C Cuts at: 2979 2979 Size: 5752 SalI G'TCGA_C Cuts at: 1936 3094 1936 Size: 1158 4594 SbfI CC_TGCA'GG Cuts at: 3104 3104 Size: 5752 ScaI AGT'ACT Cuts at: 2539 4000 5712 2539 Size: 1461 1712 2579 Fragments arranged by size: 2579 1712 1461 SfcI C'TryA_G Cuts at: 826 1045 2774 3100 4235 4916 5107 826 Size: 219 1729 326 1135 681 191 1471 Fragments arranged by size: 1729 1471 1135 681 326 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5752 SmaI CCC'GGG Cuts at: 1872 3079 1872 Size: 1207 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2120 2945 3856 4757 5019 820 Size: 197 926 177 825 911 901 262 1553 Fragments arranged by size: 1553 926 911 901 825 262 197 177 SnaBI TAC'GTA Cuts at: 493 3543 493 Size: 3050 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5752 SphI G_CATG'C Cuts at: 1132 3110 1132 Size: 1978 3774 SspI AAT'ATT Cuts at: 5 52 3676 5 Size: 47 3624 2081 Fragments arranged by size: 3624 2081 47 StuI AGG'CCT Cuts at: 1517 2592 1517 Size: 1075 4677 StyI C'CwwG_G Cuts at: 513 1065 3191 513 Size: 552 2126 3074 Fragments arranged by size: 3074 2126 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3009 3779 3796 3949 4134 4579 325 Size: 1255 1429 770 17 153 185 445 1498 Fragments arranged by size: 1498 1429 1255 770 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2098 2516 2537 Size: 274 80 33 51 1443 121 418 21 Cuts at: 2537 2965 3998 5710 96 Size: 428 1033 1712 138 Fragments arranged by size: 1712 1443 1033 428 418 274 138 121 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5712 5744 5712 Size: 32 5720 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5752 XbaI T'CTAG_A Cuts at: 3088 3088 Size: 5752 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5752 XmaI C'CCGG_G Cuts at: 1870 3077 1870 Size: 1207 4545 XmnI GAAnn'nnTTC Cuts at: 3881 3881 Size: 5752 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 276 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI