Restriction map for
pFN21AB5274
(Linear) (Six-base) MAPSORT of: KIBB5274.seq Check: 9880 from: 1 to: 1047
KIBB5274.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AccI GT'mk_AC
Cuts at: 0 203 1047
Size: 203 844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 1047
Size: 542 505
AlwNI CAG_nnn'CTG
Cuts at: 0 520 1047
Size: 520 527
ApoI r'AATT_y
Cuts at: 0 839 1047
Size: 839 208
BanII G_rGCy'C
Cuts at: 0 974 1047
Size: 974 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 454 992 1047
Size: 454 538 55
Fragments arranged by size:
538 454 55
BbvCI CC'TCA_GC
Cuts at: 0 676 1047
Size: 676 371
BciVI GTATCCnnnnn_n'
Cuts at: 0 297 1047
Size: 297 750
BglII A'GATC_T
Cuts at: 0 753 1047
Size: 753 294
BmtI G_CTAG'C
Cuts at: 0 338 1047
Size: 338 709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 55 461 587 778 1047
Size: 47 8 406 126 191 269
Fragments arranged by size:
406 269 191 126 47 8
Bpu10I CC'TnA_GC
Cuts at: 0 676 1047
Size: 676 371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 925 1047
Size: 100 825 122
Fragments arranged by size:
825 122 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 697 874 1047
Size: 697 177 173
Fragments arranged by size:
697 177 173
BsaBI GATnn'nnATC
Cuts at: 0 302 308 1047
Size: 302 6 739
Fragments arranged by size:
739 302 6
BsaHI Gr'CG_yC
Cuts at: 0 153 1047
Size: 153 894
BsaWI w'CCGG_w
Cuts at: 0 443 1047
Size: 443 604
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 58 1047
Size: 28 30 989
Fragments arranged by size:
989 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 14 337 1036 1047
Size: 14 323 699 11
Fragments arranged by size:
699 323 14 11
BseYI C'CCAG_C
Cuts at: 0 707 1047
Size: 707 340
BsiHKAI G_wGCw'C
Cuts at: 0 974 1047
Size: 974 73
Bsp1286I G_dGCh'C
Cuts at: 0 974 1047
Size: 974 73
BspEI T'CCGG_A
Cuts at: 0 443 1047
Size: 443 604
BspHI T'CATG_A
Cuts at: 0 917 1047
Size: 917 130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 1047
Size: 215 832
BstXI CCAn_nnnn'nTGG
Cuts at: 0 828 1047
Size: 828 219
BstYI r'GATC_y
Cuts at: 0 753 909 1047
Size: 753 156 138
Fragments arranged by size:
753 156 138
BtgI C'CryG_G
Cuts at: 0 399 1047
Size: 399 648
BtsI GCAGTG_nn'
Cuts at: 0 693 1047
Size: 693 354
EarI CTCTTCn'nnn_
Cuts at: 0 427 603 1047
Size: 427 176 444
Fragments arranged by size:
444 427 176
EcoICRI GAG'CTC
Cuts at: 0 972 1047
Size: 972 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 55 461 542 587 778 1047
Size: 47 8 406 81 45 191 269
Fragments arranged by size:
406 269 191 81 47 45 8
EcoRI G'AATT_C
Cuts at: 0 839 1047
Size: 839 208
EcoRV GAT'ATC
Cuts at: 0 270 594 1047
Size: 270 324 453
Fragments arranged by size:
453 324 270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 696 728 1015 1047
Size: 696 32 287 32
Fragments arranged by size:
696 287 32 32
HincII GTy'rAC
Cuts at: 0 111 1047
Size: 111 936
HindIII A'AGCT_T
Cuts at: 0 225 1047
Size: 225 822
MfeI C'AATT_G
Cuts at: 0 131 1047
Size: 131 916
MslI CAynn'nnrTG
Cuts at: 0 146 716 922 1047
Size: 146 570 206 125
Fragments arranged by size:
570 206 146 125
MspA1I CmG'CkG
Cuts at: 0 265 828 1047
Size: 265 563 219
Fragments arranged by size:
563 265 219
NheI G'CTAG_C
Cuts at: 0 334 1047
Size: 334 713
PfoI T'CCnGG_A
Cuts at: 0 603 1047
Size: 603 444
PvuII CAG'CTG
Cuts at: 0 265 828 1047
Size: 265 563 219
Fragments arranged by size:
563 265 219
SacI G_AGCT'C
Cuts at: 0 974 1047
Size: 974 73
ScaI AGT'ACT
Cuts at: 0 534 1047
Size: 534 513
SfcI C'TryA_G
Cuts at: 0 769 1047
Size: 769 278
SmlI C'TyrA_G
Cuts at: 0 115 940 1047
Size: 115 825 107
Fragments arranged by size:
825 115 107
StuI AGG'CCT
Cuts at: 0 587 1047
Size: 587 460
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1004 1047
Size: 1004 43
TatI w'GTAC_w
Cuts at: 0 93 511 532 960 1047
Size: 93 418 21 428 87
Fragments arranged by size:
428 418 93 87 21
Enzymes that do cut:
AccI AcuI AlwNI ApoI BanII BbsI BbvCI BciVI
BglII BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspEI BspHI
BspMI BstXI BstYI BtgI BtsI EarI EcoICRI Eco57MI
EcoRI EcoRV Hin4I HincII HindIII MfeI MslI MspA1I
NheI PfoI PvuII SacI ScaI SfcI SmlI StuI
TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI
BsaAI BsgI BsiEI BsiWI BsmI BsmBI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoNI EcoO109I FalI FseI FspI FspAI
HaeII HpaI KasI KpnI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StyI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI