(Circular) (Six-base) MAPSORT of: pFN21AB5263.seq Check: 5573 from: 1 to: 5842 pFN21AB5263 5842 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2543 2543 Size: 5842 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3652 278 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2358 3185 1937 Size: 421 827 4594 Fragments arranged by size: 4594 827 421 Acc65I G'GTAC_C Cuts at: 2631 3163 2631 Size: 532 5310 AclI AA'CG_TT Cuts at: 1563 3969 4342 1563 Size: 2406 373 3063 Fragments arranged by size: 3063 2406 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3104 3905 5289 5557 1632 Size: 1472 801 1384 268 1917 Fragments arranged by size: 1917 1472 1384 801 268 AfeI AGC'GCT Cuts at: 2976 2976 Size: 5842 AflII C'TTAA_G Cuts at: 820 1017 2543 820 Size: 197 1526 4119 Fragments arranged by size: 4119 1526 197 AflIII A'CryG_T Cuts at: 1203 4741 5718 1203 Size: 3538 977 1327 Fragments arranged by size: 3538 1327 977 AgeI A'CCGG_T Cuts at: 4673 4673 Size: 5842 AhdI GACnn_n'nnGTC Cuts at: 2689 4571 2689 Size: 1882 3960 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5842 AlwNI CAG_nnn'CTG Cuts at: 2278 2374 2416 2455 2854 3585 5157 2278 Size: 96 42 39 399 731 1572 2963 Fragments arranged by size: 2963 1572 731 399 96 42 39 ApaI G_GGCC'C Cuts at: 2123 2641 2123 Size: 518 5324 ApaLI G'TGCA_C Cuts at: 3899 5055 3899 Size: 1156 4686 ApoI r'AATT_y Cuts at: 1493 3151 3377 3529 5638 1493 Size: 1658 226 152 2109 1697 Fragments arranged by size: 2109 1697 1658 226 152 AseI AT'TA_AT Cuts at: 160 1032 4396 160 Size: 872 3364 1606 Fragments arranged by size: 3364 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 AvaI C'yCGr_G Cuts at: 1870 1943 2068 2077 3167 1870 Size: 73 125 9 1090 4545 Fragments arranged by size: 4545 1090 125 73 9 BamHI G'GATC_C Cuts at: 3172 3539 3172 Size: 367 5475 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2631 3163 4618 618 Size: 325 217 627 844 532 1455 1842 Fragments arranged by size: 1842 1455 844 627 532 325 217 BanII G_rGCy'C Cuts at: 1391 2046 2123 2641 2792 3039 3093 3161 1391 Size: 655 77 518 151 247 54 68 4072 Fragments arranged by size: 4072 655 518 247 151 77 68 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4871 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4033 4067 1903 Size: 34 2096 34 3678 Fragments arranged by size: 3678 2096 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3734 4944 3734 Size: 1210 4632 BclI T'GATC_A Cuts at: 1548 1548 Size: 5842 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4453 136 Size: 107 122 71 631 605 337 2444 1525 Fragments arranged by size: 2444 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2500 5837 2500 Size: 3337 2505 BlpI GC'TnA_GC Cuts at: 2417 3259 2417 Size: 842 5000 Bme1580I G_kGCm'C Cuts at: 1790 2123 2641 3903 5059 1790 Size: 333 518 1262 1156 2573 Fragments arranged by size: 2573 1262 1156 518 333 BmgBI CAC'GTC Cuts at: 1318 2116 1318 Size: 798 5044 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3573 4531 442 Size: 416 53 83 399 24 2156 958 1753 Fragments arranged by size: 2156 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5842 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2737 2737 Size: 5842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3014 3152 4502 1239 Size: 1775 138 1350 2579 Fragments arranged by size: 2579 1775 1350 138 Bpu10I CC'TnA_GC Cuts at: 2022 2583 2022 Size: 561 5281 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2450 3967 4832 5130 2450 Size: 1517 865 298 3162 Fragments arranged by size: 3162 1517 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4505 882 Size: 617 3006 2219 Fragments arranged by size: 3006 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2191 3633 493 Size: 711 987 1442 2702 Fragments arranged by size: 2702 1442 987 711 BsaBI GATnn'nnATC Cuts at: 2273 5836 2273 Size: 3563 2279 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2330 3649 4031 4666 Size: 53 83 186 936 797 1319 382 635 Cuts at: 4666 275 Size: 1451 Fragments arranged by size: 1451 1319 936 797 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2928 4275 4673 4947 5094 5505 2928 Size: 1347 398 274 147 411 3265 Fragments arranged by size: 3265 1347 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2462 2492 1178 Size: 30 165 30 1059 30 4528 Fragments arranged by size: 4528 1059 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3047 1186 Size: 1861 3981 BseYI C'CCAG_C Cuts at: 5045 5541 5045 Size: 496 5346 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2387 1163 Size: 573 651 4618 Fragments arranged by size: 4618 651 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3292 4053 5081 1540 Size: 275 186 1291 761 1028 2301 Fragments arranged by size: 2301 1291 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2493 3093 3903 3988 5059 2493 Size: 600 810 85 1071 3276 Fragments arranged by size: 3276 1071 810 600 85 BsiWI C'GTAC_G Cuts at: 2160 2160 Size: 5842 BsmI GAATG_Cn' Cuts at: 2703 3353 3446 2703 Size: 650 93 5099 Fragments arranged by size: 5099 650 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2123 2493 2641 2792 3039 3093 Size: 399 256 77 370 148 151 247 54 Cuts at: 3093 3161 3903 3988 5059 1391 Size: 68 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 370 256 247 151 148 85 77 68 54 BspEI T'CCGG_A Cuts at: 5505 5505 Size: 5842 BspHI T'CATG_A Cuts at: 3731 5461 3731 Size: 1730 4112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2526 2543 2928 3197 844 Size: 1682 17 385 269 3489 Fragments arranged by size: 3489 1682 385 269 17 BsrBI CCG'CTC Cuts at: 1394 1823 3079 3729 1394 Size: 429 1256 650 3507 Fragments arranged by size: 3507 1256 650 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2428 4337 4511 65 Size: 1183 1180 1909 174 1396 Fragments arranged by size: 1909 1396 1183 1180 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2597 2727 4486 4673 1700 Size: 110 787 130 1759 187 2869 Fragments arranged by size: 2869 1759 787 187 130 110 BsrGI T'GTAC_A Cuts at: 96 2391 96 Size: 2295 3547 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5842 BssSI C'ACGA_G Cuts at: 3902 4914 3902 Size: 1012 4830 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5842 BstBI TT'CG_AA Cuts at: 2232 4646 2232 Size: 2414 3428 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5842 BstXI CCAn_nnnn'nTGG Cuts at: 3058 3058 Size: 5842 BstYI r'GATC_y Cuts at: 1972 2268 2274 2451 2500 3172 3539 3924 3941 Size: 296 6 177 49 672 367 385 17 Cuts at: 3941 5393 5479 5491 5837 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 672 385 367 346 296 177 86 49 17 12 6 Bsu36I CC'TnA_GG Cuts at: 3072 3072 Size: 5842 BtgI C'CryG_G Cuts at: 513 1065 2004 3051 513 Size: 552 939 1047 3304 Fragments arranged by size: 3304 1047 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5103 Fragments arranged by size: 5103 683 56 BtsI GCAGTG_nn' Cuts at: 3358 4152 4172 3358 Size: 794 20 5028 Fragments arranged by size: 5028 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5842 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5807 DraI TTT'AAA Cuts at: 3146 3146 Size: 5842 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4849 809 Size: 507 3533 1802 Fragments arranged by size: 3533 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 3027 3289 Size: 54 1425 50 275 18 378 819 262 Cuts at: 3289 4178 5807 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 819 378 275 262 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3289 1537 Size: 275 1477 4090 Fragments arranged by size: 4090 1477 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3772 1353 Size: 2419 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2279 2470 4429 4803 4949 387 Size: 1064 138 690 191 1959 374 146 1280 Fragments arranged by size: 1959 1280 1064 690 374 191 146 138 EcoICRI GAG'CTC Cuts at: 3091 3091 Size: 5842 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3014 3104 3152 3905 4502 5289 5557 Size: 393 1382 90 48 753 597 787 268 Cuts at: 5557 1239 Size: 1524 Fragments arranged by size: 1524 1382 787 753 597 393 268 90 48 EcoNI CCTnn'n_nnAGG Cuts at: 3071 3071 Size: 5842 EcoO109I rG'GnC_Cy Cuts at: 2074 2371 2461 2638 2806 3136 2074 Size: 297 90 177 168 330 4780 Fragments arranged by size: 4780 330 297 177 168 90 EcoRI G'AATT_C Cuts at: 3151 3529 3151 Size: 378 5464 FspI TGC'GCA Cuts at: 2195 4348 2195 Size: 2153 3689 HaeII r_GCGC'y Cuts at: 1678 2978 4989 1678 Size: 1300 2011 2531 Fragments arranged by size: 2531 2011 1300 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2563 2595 2675 2707 4483 Size: 32 135 32 991 32 80 32 1776 Cuts at: 4483 4515 4557 4589 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1776 991 135 80 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2937 3186 3432 669 Size: 988 281 999 249 246 3079 Fragments arranged by size: 3079 999 988 281 249 246 HindIII A'AGCT_T Cuts at: 748 2885 748 Size: 2137 3705 HpaI GTT'AAC Cuts at: 2937 3432 2937 Size: 495 5347 KpnI G_GTAC'C Cuts at: 2635 3167 2635 Size: 532 5310 MfeI C'AATT_G Cuts at: 3441 3441 Size: 5842 MluI A'CGCG_T Cuts at: 5718 5718 Size: 5842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2538 4956 5140 1305 Size: 1233 2418 184 2007 Fragments arranged by size: 2418 2007 1233 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 10 Size: 54 1425 343 378 3642 Fragments arranged by size: 3642 1425 378 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2298 2825 3800 4159 4318 518 Size: 648 1132 527 975 359 159 2042 Fragments arranged by size: 2042 1132 975 648 527 359 159 MspA1I CmG'CkG Cuts at: 1606 2416 3258 3935 5083 5328 1606 Size: 810 842 677 1148 245 2120 Fragments arranged by size: 2120 1148 842 810 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3051 513 Size: 552 939 1047 3304 Fragments arranged by size: 3304 1047 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5842 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5842 NotI GC'GGCC_GC Cuts at: 3289 3289 Size: 5842 NspI r_CATG'y Cuts at: 1132 1229 3200 4745 1132 Size: 97 1971 1545 2229 Fragments arranged by size: 2229 1971 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5842 PciI A'CATG_T Cuts at: 4741 4741 Size: 5842 PflMI CCAn_nnn'nTGG Cuts at: 1108 2776 1108 Size: 1668 4174 PfoI T'CCnGG_A Cuts at: 2262 3099 2262 Size: 837 5005 PmeI GTTT'AAAC Cuts at: 3146 3146 Size: 5842 PmlI CAC'GTG Cuts at: 2191 2191 Size: 5842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3911 3943 5424 5456 3911 Size: 32 1481 32 4297 Fragments arranged by size: 4297 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2074 2371 2806 2074 Size: 297 435 5110 Fragments arranged by size: 5110 435 297 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5842 PsiI TTA'TAA Cuts at: 3412 3412 Size: 5842 PspOMI G'GGCC_C Cuts at: 2119 2637 2119 Size: 518 5324 PstI C_TGCA'G Cuts at: 830 3194 830 Size: 2364 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5842 PvuII CAG'CTG Cuts at: 2416 2416 Size: 5842 SacI G_AGCT'C Cuts at: 3093 3093 Size: 5842 SalI G'TCGA_C Cuts at: 1936 3184 1936 Size: 1248 4594 SanDI GG'GwC_CC Cuts at: 2074 2074 Size: 5842 SbfI CC_TGCA'GG Cuts at: 3194 3194 Size: 5842 ScaI AGT'ACT Cuts at: 3002 4090 5802 3002 Size: 1088 1712 3042 Fragments arranged by size: 3042 1712 1088 SfcI C'TryA_G Cuts at: 826 1045 2846 3190 4325 5006 5197 826 Size: 219 1801 344 1135 681 191 1471 Fragments arranged by size: 1801 1471 1135 681 344 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5842 SmaI CCC'GGG Cuts at: 1872 2070 2079 3169 1872 Size: 198 9 1090 4545 Fragments arranged by size: 4545 1090 198 9 SmlI C'TyrA_G Cuts at: 820 1017 1943 2465 2543 3946 4847 5109 820 Size: 197 926 522 78 1403 901 262 1553 Fragments arranged by size: 1553 1403 926 901 522 262 197 78 SnaBI TAC'GTA Cuts at: 493 3633 493 Size: 3140 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5842 SphI G_CATG'C Cuts at: 1132 3200 1132 Size: 2068 3774 SspI AAT'ATT Cuts at: 5 52 3766 5 Size: 47 3714 2081 Fragments arranged by size: 3714 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5842 StyI C'CwwG_G Cuts at: 513 1065 2004 2661 2868 3051 3281 513 Size: 552 939 657 207 183 230 3074 Fragments arranged by size: 3074 939 657 552 230 207 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3869 3886 4039 4224 4669 325 Size: 1255 2289 17 153 185 445 1498 Fragments arranged by size: 2289 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2142 2391 2469 Size: 274 80 33 51 1443 165 249 78 Cuts at: 2469 3000 4088 5800 96 Size: 531 1088 1712 138 Fragments arranged by size: 1712 1443 1088 531 274 249 165 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5802 5834 5802 Size: 32 5810 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5842 XbaI T'CTAG_A Cuts at: 3178 3178 Size: 5842 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2869 2869 Size: 5842 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5842 XmaI C'CCGG_G Cuts at: 1870 2068 2077 3167 1870 Size: 198 9 1090 4545 Fragments arranged by size: 4545 1090 198 9 XmnI GAAnn'nnTTC Cuts at: 3971 3971 Size: 5842 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3650 276 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI