(Circular) (Six-base) MAPSORT of: pFN21AB5234.seq Check: 7466 from: 1 to: 6307 pFN21AB5234 6307 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4117 278 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2471 3650 1937 Size: 534 1179 4594 Fragments arranged by size: 4594 1179 534 Acc65I G'GTAC_C Cuts at: 2865 3628 2865 Size: 763 5544 AclI AA'CG_TT Cuts at: 1563 4434 4807 1563 Size: 2871 373 3063 Fragments arranged by size: 3063 2871 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2508 4370 5754 6022 1632 Size: 876 1862 1384 268 1917 Fragments arranged by size: 1917 1862 1384 876 268 AfeI AGC'GCT Cuts at: 2543 3068 2543 Size: 525 5782 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6110 AflIII A'CryG_T Cuts at: 1203 3468 5206 6183 1203 Size: 2265 1738 977 1327 Fragments arranged by size: 2265 1738 1327 977 AgeI A'CCGG_T Cuts at: 5138 5138 Size: 6307 AhdI GACnn_n'nnGTC Cuts at: 3520 5036 3520 Size: 1516 4791 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6307 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2711 1184 Size: 1527 4780 AlwNI CAG_nnn'CTG Cuts at: 2183 2432 3139 3280 4050 5622 2183 Size: 249 707 141 770 1572 2868 Fragments arranged by size: 2868 1572 770 707 249 141 ApaI G_GGCC'C Cuts at: 2296 2611 2296 Size: 315 5992 ApaLI G'TGCA_C Cuts at: 2987 4364 5520 2987 Size: 1377 1156 3774 Fragments arranged by size: 3774 1377 1156 ApoI r'AATT_y Cuts at: 1493 3616 3842 3994 6103 1493 Size: 2123 226 152 2109 1697 Fragments arranged by size: 2123 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4861 160 Size: 872 3829 1606 Fragments arranged by size: 3829 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 AvaI C'yCGr_G Cuts at: 1870 1943 2385 2649 3411 3632 1870 Size: 73 442 264 762 221 4545 Fragments arranged by size: 4545 762 442 264 221 73 BamHI G'GATC_C Cuts at: 3637 4004 3637 Size: 367 5940 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2028 2865 3628 5083 618 Size: 325 217 627 241 837 763 1455 1842 Fragments arranged by size: 1842 1455 837 763 627 325 241 217 BanII G_rGCy'C Cuts at: 1391 2021 2296 2418 2611 3420 3626 1391 Size: 630 275 122 193 809 206 4072 Fragments arranged by size: 4072 809 630 275 206 193 122 BbeI G_GCGC'C Cuts at: 2032 2032 Size: 6307 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5336 BbvCI CC'TCA_GC Cuts at: 2955 2955 Size: 6307 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2266 2300 4498 4532 1903 Size: 34 329 34 2198 34 3678 Fragments arranged by size: 3678 2198 329 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4199 5409 4199 Size: 1210 5097 BclI T'GATC_A Cuts at: 1548 3231 1548 Size: 1683 4624 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4918 136 Size: 107 122 71 631 605 3246 1525 Fragments arranged by size: 3246 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2562 6302 2562 Size: 3740 2567 BlpI GC'TnA_GC Cuts at: 2184 3724 2184 Size: 1540 4767 Bme1580I G_kGCm'C Cuts at: 1790 2296 2611 2991 4368 5524 1790 Size: 506 315 380 1377 1156 2573 Fragments arranged by size: 2573 1377 1156 506 380 315 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6307 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4038 4996 442 Size: 416 53 83 399 24 2621 958 1753 Fragments arranged by size: 2621 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3428 1056 Size: 2372 3935 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3510 3510 Size: 6307 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2924 4967 1239 Size: 1685 2043 2579 Fragments arranged by size: 2579 2043 1685 Bpu10I CC'TnA_GC Cuts at: 2955 2955 Size: 6307 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3047 3081 4432 5297 5595 3047 Size: 34 1351 865 298 3759 Fragments arranged by size: 3759 1351 865 298 34 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4970 882 Size: 617 3471 2219 Fragments arranged by size: 3471 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2239 4098 493 Size: 711 1035 1859 2702 Fragments arranged by size: 2702 1859 1035 711 BsaBI GATnn'nnATC Cuts at: 3398 6301 3398 Size: 2903 3404 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2029 4114 4496 5131 Size: 53 83 186 936 496 2085 382 635 Cuts at: 5131 275 Size: 1451 Fragments arranged by size: 2085 1451 936 635 496 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2709 4740 5138 5412 5559 5970 2709 Size: 2031 398 274 147 411 3046 Fragments arranged by size: 3046 2031 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6082 Fragments arranged by size: 6082 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2082 2135 2501 1186 Size: 896 53 366 4992 Fragments arranged by size: 4992 896 366 53 BseYI C'CCAG_C Cuts at: 2193 3019 3420 5510 6006 2193 Size: 826 401 2090 496 2494 Fragments arranged by size: 2494 2090 826 496 401 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2375 1163 Size: 573 639 5095 Fragments arranged by size: 5095 639 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3757 4518 5546 1540 Size: 275 186 1756 761 1028 2301 Fragments arranged by size: 2301 1756 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 2238 2991 4368 4453 5524 2190 Size: 48 753 1377 85 1071 2973 Fragments arranged by size: 2973 1377 1071 753 85 48 BsmI GAATG_Cn' Cuts at: 3818 3911 3818 Size: 93 6214 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2190 2238 2296 2418 2611 2991 Size: 399 231 169 48 58 122 193 380 Cuts at: 2991 3420 3626 4368 4453 5524 1391 Size: 429 206 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 429 399 380 231 206 193 169 122 85 58 48 BspEI T'CCGG_A Cuts at: 2709 5970 2709 Size: 3261 3046 BspHI T'CATG_A Cuts at: 2526 4196 5926 2526 Size: 1670 1730 2907 Fragments arranged by size: 2907 1730 1670 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3662 844 Size: 2818 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4194 1394 Size: 429 2371 3507 Fragments arranged by size: 3507 2371 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4802 4976 65 Size: 1183 3554 174 1396 Fragments arranged by size: 3554 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2092 3036 4951 5138 1700 Size: 110 282 944 1915 187 2869 Fragments arranged by size: 2869 1915 944 282 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6307 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6307 BssSI C'ACGA_G Cuts at: 2513 3362 4367 5379 2513 Size: 849 1005 1012 3441 Fragments arranged by size: 3441 1012 1005 849 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6307 BstBI TT'CG_AA Cuts at: 5111 5111 Size: 6307 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6307 BstXI CCAn_nnnn'nTGG Cuts at: 2902 3577 2902 Size: 675 5632 BstYI r'GATC_y Cuts at: 1972 2562 3027 3637 4004 4389 4406 5858 5944 Size: 590 465 610 367 385 17 1452 86 Cuts at: 5944 5956 6302 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 610 590 465 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2032 2418 513 Size: 552 967 386 4402 Fragments arranged by size: 4402 967 552 386 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3253 487 Size: 683 56 2027 3541 Fragments arranged by size: 3541 2027 683 56 BtsI GCAGTG_nn' Cuts at: 3823 4617 4637 3823 Size: 794 20 5493 Fragments arranged by size: 5493 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6307 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6272 DraI TTT'AAA Cuts at: 3611 3611 Size: 6307 DraIII CAC_nnn'GTG Cuts at: 3208 3208 Size: 6307 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5314 809 Size: 507 3998 1802 Fragments arranged by size: 3998 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3754 4643 6272 Size: 54 1425 50 275 18 1924 889 1629 Cuts at: 6272 8 Size: 43 Fragments arranged by size: 1924 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3754 1537 Size: 275 1942 4090 Fragments arranged by size: 4090 1942 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2406 4237 1353 Size: 1053 1831 3423 Fragments arranged by size: 3423 1831 1053 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2079 3038 4894 5268 5414 387 Size: 1064 138 490 959 1856 374 146 1280 Fragments arranged by size: 1856 1280 1064 959 490 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2508 2924 4370 4967 5754 6022 1239 Size: 393 876 416 1446 597 787 268 1524 Fragments arranged by size: 1524 1446 876 787 597 416 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2945 2945 Size: 6307 EcoO109I rG'GnC_Cy Cuts at: 2293 2608 2950 3136 3174 2293 Size: 315 342 186 38 5426 Fragments arranged by size: 5426 342 315 186 38 EcoRI G'AATT_C Cuts at: 3616 3994 3616 Size: 378 5929 EcoRV GAT'ATC Cuts at: 3460 3460 Size: 6307 FspI TGC'GCA Cuts at: 2687 4813 2687 Size: 2126 4181 HaeII r_GCGC'y Cuts at: 1678 2032 2545 3070 5454 1678 Size: 354 513 525 2384 2531 Fragments arranged by size: 2531 2384 525 513 354 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2578 2610 3506 3538 4948 Size: 32 135 32 1006 32 896 32 1410 Cuts at: 4948 4980 5022 5054 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1410 1006 896 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2145 2472 3651 3897 669 Size: 988 281 207 327 1179 246 3079 Fragments arranged by size: 3079 1179 988 327 281 246 207 HindIII A'AGCT_T Cuts at: 748 2993 748 Size: 2245 4062 HpaI GTT'AAC Cuts at: 3897 3897 Size: 6307 KasI G'GCGC_C Cuts at: 2028 2028 Size: 6307 KpnI G_GTAC'C Cuts at: 2869 3632 2869 Size: 763 5544 MfeI C'AATT_G Cuts at: 3906 3906 Size: 6307 MluI A'CGCG_T Cuts at: 6183 6183 Size: 6307 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2530 3267 5421 5605 1305 Size: 1225 737 2154 184 2007 Fragments arranged by size: 2154 2007 1225 737 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4485 Fragments arranged by size: 4485 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2525 3005 4265 4624 4783 518 Size: 648 1359 480 1260 359 159 2042 Fragments arranged by size: 2042 1359 1260 648 480 359 159 MspA1I CmG'CkG Cuts at: 1606 2034 2180 2902 2966 3723 4400 5548 5793 Size: 428 146 722 64 757 677 1148 245 Cuts at: 5793 1606 Size: 2120 Fragments arranged by size: 2120 1148 757 722 677 428 245 146 64 NaeI GCC'GGC Cuts at: 2094 2094 Size: 6307 NarI GG'CG_CC Cuts at: 2029 2029 Size: 6307 NcoI C'CATG_G Cuts at: 513 1065 2418 513 Size: 552 1353 4402 Fragments arranged by size: 4402 1353 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6307 NgoMIV G'CCGG_C Cuts at: 2092 2092 Size: 6307 NheI G'CTAG_C Cuts at: 1052 3424 1052 Size: 2372 3935 NotI GC'GGCC_GC Cuts at: 3754 3754 Size: 6307 NspI r_CATG'y Cuts at: 1132 1229 2702 3472 3665 5210 1132 Size: 97 1473 770 193 1545 2229 Fragments arranged by size: 2229 1545 1473 770 193 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6307 PciI A'CATG_T Cuts at: 3468 5206 3468 Size: 1738 4569 PflMI CCAn_nnn'nTGG Cuts at: 1108 2107 3139 1108 Size: 999 1032 4276 Fragments arranged by size: 4276 1032 999 PfoI T'CCnGG_A Cuts at: 2406 2664 3138 3330 2406 Size: 258 474 192 5383 Fragments arranged by size: 5383 474 258 192 PmeI GTTT'AAAC Cuts at: 3611 3611 Size: 6307 PmlI CAC'GTG Cuts at: 2239 2239 Size: 6307 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4376 4408 5889 5921 4376 Size: 32 1481 32 4762 Fragments arranged by size: 4762 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3136 3174 3136 Size: 38 6269 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6307 PsiI TTA'TAA Cuts at: 3877 3877 Size: 6307 PspOMI G'GGCC_C Cuts at: 2292 2607 2292 Size: 315 5992 PstI C_TGCA'G Cuts at: 830 3045 3285 3529 3659 830 Size: 2215 240 244 130 3478 Fragments arranged by size: 3478 2215 244 240 130 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6307 PvuII CAG'CTG Cuts at: 2902 2966 2902 Size: 64 6243 SacII CC_GC'GG Cuts at: 2035 2035 Size: 6307 SalI G'TCGA_C Cuts at: 1936 2470 3649 1936 Size: 534 1179 4594 Fragments arranged by size: 4594 1179 534 SapI GCTCTTCn'nnn_ Cuts at: 2406 2406 Size: 6307 SbfI CC_TGCA'GG Cuts at: 3659 3659 Size: 6307 ScaI AGT'ACT Cuts at: 2593 2909 4555 6267 2593 Size: 316 1646 1712 2633 Fragments arranged by size: 2633 1712 1646 316 SexAI A'CCwGG_T Cuts at: 3132 3132 Size: 6307 SfcI C'TryA_G Cuts at: 826 1045 2785 3041 3281 3525 3655 4790 5471 Size: 219 1740 256 240 244 130 1135 681 Cuts at: 5471 5662 826 Size: 191 1471 Fragments arranged by size: 1740 1471 1135 681 256 244 240 219 191 130 SfoI GGC'GCC Cuts at: 2030 2030 Size: 6307 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6307 SmaI CCC'GGG Cuts at: 1872 2651 3634 1872 Size: 779 983 4545 Fragments arranged by size: 4545 983 779 SmlI C'TyrA_G Cuts at: 820 1017 1943 2385 3062 3096 4411 5312 5574 Size: 197 926 442 677 34 1315 901 262 Cuts at: 5574 820 Size: 1553 Fragments arranged by size: 1553 1315 926 901 677 442 262 197 34 SnaBI TAC'GTA Cuts at: 493 4098 493 Size: 3605 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6307 SphI G_CATG'C Cuts at: 1132 2702 3665 1132 Size: 1570 963 3774 Fragments arranged by size: 3774 1570 963 SspI AAT'ATT Cuts at: 5 52 4231 5 Size: 47 4179 2081 Fragments arranged by size: 4179 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6307 StyI C'CwwG_G Cuts at: 513 1065 2217 2418 3258 3321 3384 3570 3746 Size: 552 1152 201 840 63 63 186 176 Cuts at: 3746 513 Size: 3074 Fragments arranged by size: 3074 1152 840 552 201 186 176 63 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4334 4351 4504 4689 5134 325 Size: 1255 2754 17 153 185 445 1498 Fragments arranged by size: 2754 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2591 2907 4553 Size: 274 80 33 51 1443 614 316 1646 Cuts at: 4553 6265 96 Size: 1712 138 Fragments arranged by size: 1712 1646 1443 614 316 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6267 6299 6267 Size: 32 6275 Tth111I GACn'n_nGTC Cuts at: 1316 2210 1316 Size: 894 5413 XbaI T'CTAG_A Cuts at: 3643 3643 Size: 6307 XhoI C'TCGA_G Cuts at: 1943 2385 1943 Size: 442 5865 XmaI C'CCGG_G Cuts at: 1870 2649 3632 1870 Size: 779 983 4545 Fragments arranged by size: 4545 983 779 XmnI GAAnn'nnTTC Cuts at: 4436 4436 Size: 6307 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4115 276 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SanDI SfiI SrfI SwaI XcmI