(Circular) (Six-base) MAPSORT of: pFN21AB5212.seq Check: 7296 from: 1 to: 5887 pFN21AB5212 5887 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3104 3104 Size: 5887 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3697 278 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2112 3230 1937 Size: 175 1118 4594 Fragments arranged by size: 4594 1118 175 Acc65I G'GTAC_C Cuts at: 3208 3208 Size: 5887 AclI AA'CG_TT Cuts at: 1563 4014 4387 1563 Size: 2451 373 3063 Fragments arranged by size: 3063 2451 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3950 5334 5602 1632 Size: 2318 1384 268 1917 Fragments arranged by size: 2318 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5690 AflIII A'CryG_T Cuts at: 1203 2664 3001 4786 5763 1203 Size: 1461 337 1785 977 1327 Fragments arranged by size: 1785 1461 1327 977 337 AgeI A'CCGG_T Cuts at: 4718 4718 Size: 5887 AhdI GACnn_n'nnGTC Cuts at: 4616 4616 Size: 5887 AleI CACnn'nnGTG Cuts at: 1166 2171 1166 Size: 1005 4882 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2360 1184 Size: 1176 4711 AlwNI CAG_nnn'CTG Cuts at: 2593 3630 5202 2593 Size: 1037 1572 3278 Fragments arranged by size: 3278 1572 1037 ApaLI G'TGCA_C Cuts at: 3944 5100 3944 Size: 1156 4731 ApoI r'AATT_y Cuts at: 1493 2438 2945 3196 3422 3574 5683 1493 Size: 945 507 251 226 152 2109 1697 Fragments arranged by size: 2109 1697 945 507 251 226 152 AseI AT'TA_AT Cuts at: 160 1032 4441 160 Size: 872 3409 1606 Fragments arranged by size: 3409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2369 2981 3009 3212 1870 Size: 73 72 354 612 28 203 4545 Fragments arranged by size: 4545 612 354 203 73 72 28 BamHI G'GATC_C Cuts at: 2187 2706 3217 3584 2187 Size: 519 511 367 4490 Fragments arranged by size: 4490 519 511 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2344 3208 4663 618 Size: 325 217 627 557 864 1455 1842 Fragments arranged by size: 1842 1455 864 627 557 325 217 BanII G_rGCy'C Cuts at: 1391 2988 3206 1391 Size: 1597 218 4072 Fragments arranged by size: 4072 1597 218 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4916 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2711 2745 4078 4112 1903 Size: 34 774 34 1333 34 3678 Fragments arranged by size: 3678 1333 774 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3779 4989 3779 Size: 1210 4677 BclI T'GATC_A Cuts at: 1548 2556 1548 Size: 1008 4879 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4498 136 Size: 107 122 71 631 605 2826 1525 Fragments arranged by size: 2826 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2887 5882 2887 Size: 2995 2892 BlpI GC'TnA_GC Cuts at: 3304 3304 Size: 5887 Bme1580I G_kGCm'C Cuts at: 1790 2349 2530 3948 5104 1790 Size: 559 181 1418 1156 2573 Fragments arranged by size: 2573 1418 1156 559 181 BmgBI CAC'GTC Cuts at: 1318 2554 2674 1318 Size: 1236 120 4531 Fragments arranged by size: 4531 1236 120 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2524 3618 4576 Size: 416 53 83 399 24 1107 1094 958 Cuts at: 4576 442 Size: 1753 Fragments arranged by size: 1753 1107 1094 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2169 4547 1239 Size: 930 2378 2579 Fragments arranged by size: 2579 2378 930 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4012 4877 5175 4012 Size: 865 298 4724 Fragments arranged by size: 4724 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2624 4550 882 Size: 617 1125 1926 2219 Fragments arranged by size: 2219 1926 1125 617 BsaAI yAC'GTr Cuts at: 493 1204 3678 493 Size: 711 2474 2702 Fragments arranged by size: 2702 2474 711 BsaBI GATnn'nnATC Cuts at: 2186 5881 2186 Size: 3695 2192 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3070 3694 4076 4711 Size: 53 83 186 936 1537 624 382 635 Cuts at: 4711 275 Size: 1451 Fragments arranged by size: 1537 1451 936 635 624 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4320 4718 4992 5139 5550 4320 Size: 398 274 147 411 4657 Fragments arranged by size: 4657 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2483 2513 1178 Size: 30 165 30 1080 30 4552 Fragments arranged by size: 4552 1080 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 1186 Size: 877 5010 BseYI C'CCAG_C Cuts at: 2140 2912 5090 5586 2140 Size: 772 2178 496 2441 Fragments arranged by size: 2441 2178 772 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 1163 Size: 573 564 4750 Fragments arranged by size: 4750 573 564 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3337 4098 5126 1540 Size: 275 186 1336 761 1028 2301 Fragments arranged by size: 2301 1336 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3948 4033 5104 3948 Size: 85 1071 4731 Fragments arranged by size: 4731 1071 85 BsmI GAATG_Cn' Cuts at: 3398 3491 3398 Size: 93 5794 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2349 2530 2988 3206 3948 4033 5104 Size: 399 559 181 458 218 742 85 1071 Cuts at: 5104 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 559 458 399 218 181 85 BspEI T'CCGG_A Cuts at: 5550 5550 Size: 5887 BspHI T'CATG_A Cuts at: 3776 5506 3776 Size: 1730 4157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2098 2601 3104 3242 844 Size: 1254 503 503 138 3489 Fragments arranged by size: 3489 1254 503 503 138 BsrBI CCG'CTC Cuts at: 1394 1823 3774 1394 Size: 429 1951 3507 Fragments arranged by size: 3507 1951 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4382 4556 65 Size: 1183 3134 174 1396 Fragments arranged by size: 3134 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4531 4718 1700 Size: 110 2721 187 2869 Fragments arranged by size: 2869 2721 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5887 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5887 BssSI C'ACGA_G Cuts at: 3947 4959 3947 Size: 1012 4875 BstAPI GCAn_nnn'nTGC Cuts at: 794 2131 2890 794 Size: 1337 759 3791 Fragments arranged by size: 3791 1337 759 BstBI TT'CG_AA Cuts at: 4691 4691 Size: 5887 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5887 BstYI r'GATC_y Cuts at: 1972 2187 2706 2887 3217 3584 3969 3986 5438 Size: 215 519 181 330 367 385 17 1452 Cuts at: 5438 5524 5536 5882 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 519 385 367 346 330 215 181 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2967 2967 Size: 5887 BtgI C'CryG_G Cuts at: 513 1065 2004 2352 513 Size: 552 939 348 4048 Fragments arranged by size: 4048 939 552 348 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2271 2703 487 Size: 683 56 1045 432 3671 Fragments arranged by size: 3671 1045 683 432 56 BtsI GCAGTG_nn' Cuts at: 3051 3403 4197 4217 3051 Size: 352 794 20 4721 Fragments arranged by size: 4721 794 352 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5852 DraI TTT'AAA Cuts at: 3191 3191 Size: 5887 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2108 4894 809 Size: 507 792 2786 1802 Fragments arranged by size: 2786 1802 792 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2325 2361 2680 Size: 54 1425 50 275 18 495 36 319 Cuts at: 2680 2748 3334 4223 5852 8 Size: 68 586 889 1629 43 Fragments arranged by size: 1629 1425 889 586 495 319 275 68 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 3334 1537 Size: 275 1522 4090 Fragments arranged by size: 4090 1522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2220 2825 3817 1353 Size: 867 605 992 3423 Fragments arranged by size: 3423 992 867 605 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3110 4474 4848 4994 387 Size: 1064 138 1521 1364 374 146 1280 Fragments arranged by size: 1521 1364 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2169 3950 4547 5334 5602 1239 Size: 393 537 1781 597 787 268 1524 Fragments arranged by size: 1781 1524 787 597 537 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2303 2804 2303 Size: 501 5386 EcoO109I rG'GnC_Cy Cuts at: 2230 2590 2995 2230 Size: 360 405 5122 Fragments arranged by size: 5122 405 360 EcoRI G'AATT_C Cuts at: 2438 3196 3574 2438 Size: 758 378 4751 Fragments arranged by size: 4751 758 378 FspI TGC'GCA Cuts at: 2852 4393 2852 Size: 1541 4346 HaeII r_GCGC'y Cuts at: 1678 5034 1678 Size: 3356 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4528 4560 4602 4634 1373 Size: 32 135 32 2956 32 42 32 2626 Fragments arranged by size: 2956 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2656 3231 3477 669 Size: 988 281 718 575 246 3079 Fragments arranged by size: 3079 988 718 575 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5887 HpaI GTT'AAC Cuts at: 3477 3477 Size: 5887 KpnI G_GTAC'C Cuts at: 3212 3212 Size: 5887 MfeI C'AATT_G Cuts at: 3486 3486 Size: 5887 MluI A'CGCG_T Cuts at: 3001 5763 3001 Size: 2762 3125 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2526 5001 5185 1305 Size: 1221 2475 184 2007 Fragments arranged by size: 2475 2007 1221 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 2682 2750 10 Size: 54 1425 343 531 319 68 3147 Fragments arranged by size: 3147 1425 531 343 319 68 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2199 2741 2861 3845 4204 4363 Size: 648 1005 28 542 120 984 359 159 Cuts at: 4363 518 Size: 2042 Fragments arranged by size: 2042 1005 984 648 542 359 159 120 28 MspA1I CmG'CkG Cuts at: 1606 2092 2515 3303 3980 5128 5373 1606 Size: 486 423 788 677 1148 245 2120 Fragments arranged by size: 2120 1148 788 677 486 423 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4396 Fragments arranged by size: 4396 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5887 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5887 NotI GC'GGCC_GC Cuts at: 3334 3334 Size: 5887 NspI r_CATG'y Cuts at: 1132 1229 2198 2453 2668 3163 3245 4790 1132 Size: 97 969 255 215 495 82 1545 2229 Fragments arranged by size: 2229 1545 969 495 255 215 97 82 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5887 PciI A'CATG_T Cuts at: 2664 4786 2664 Size: 2122 3765 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5887 PfoI T'CCnGG_A Cuts at: 2538 2775 2538 Size: 237 5650 PmeI GTTT'AAAC Cuts at: 3191 3191 Size: 5887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2363 2395 2621 2653 3956 3988 5469 5501 2363 Size: 32 226 32 1303 32 1481 32 2749 Fragments arranged by size: 2749 1481 1303 226 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2230 2590 2995 2230 Size: 360 405 5122 Fragments arranged by size: 5122 405 360 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5887 PsiI TTA'TAA Cuts at: 3457 3457 Size: 5887 PstI C_TGCA'G Cuts at: 830 2097 2805 3239 830 Size: 1267 708 434 3478 Fragments arranged by size: 3478 1267 708 434 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5887 PvuII CAG'CTG Cuts at: 2092 2515 2092 Size: 423 5464 SalI G'TCGA_C Cuts at: 1936 3229 1936 Size: 1293 4594 SbfI CC_TGCA'GG Cuts at: 2805 3239 2805 Size: 434 5453 ScaI AGT'ACT Cuts at: 2534 3020 4135 5847 2534 Size: 486 1115 1712 2574 Fragments arranged by size: 2574 1712 1115 486 SexAI A'CCwGG_T Cuts at: 2340 2340 Size: 5887 SfcI C'TryA_G Cuts at: 826 1045 2093 2801 3235 4370 5051 5242 826 Size: 219 1048 708 434 1135 681 191 1471 Fragments arranged by size: 1471 1135 1048 708 681 434 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5887 SmaI CCC'GGG Cuts at: 1872 3011 3214 1872 Size: 1139 203 4545 Fragments arranged by size: 4545 1139 203 SmlI C'TyrA_G Cuts at: 820 1017 1943 3991 4892 5154 820 Size: 197 926 2048 901 262 1553 Fragments arranged by size: 2048 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3678 493 Size: 3185 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5887 SphI G_CATG'C Cuts at: 1132 2453 3163 3245 1132 Size: 1321 710 82 3774 Fragments arranged by size: 3774 1321 710 82 SspI AAT'ATT Cuts at: 5 52 3811 5 Size: 47 3759 2081 Fragments arranged by size: 3759 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5887 StyI C'CwwG_G Cuts at: 513 1065 2004 2454 3326 513 Size: 552 939 450 872 3074 Fragments arranged by size: 3074 939 872 552 450 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2045 2132 2666 3914 3931 4084 4269 Size: 1255 465 87 534 1248 17 153 185 Cuts at: 4269 4714 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1248 534 465 445 185 153 87 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2532 3018 4133 Size: 274 80 33 51 1443 555 486 1115 Cuts at: 4133 5845 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1115 555 486 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5847 5879 5847 Size: 32 5855 Tth111I GACn'n_nGTC Cuts at: 1316 2651 1316 Size: 1335 4552 XbaI T'CTAG_A Cuts at: 3223 3223 Size: 5887 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2338 2641 2740 2338 Size: 303 99 5485 Fragments arranged by size: 5485 303 99 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5887 XmaI C'CCGG_G Cuts at: 1870 3009 3212 1870 Size: 1139 203 4545 Fragments arranged by size: 4545 1139 203 XmnI GAAnn'nnTTC Cuts at: 2378 2819 4016 2378 Size: 441 1197 4249 Fragments arranged by size: 4249 1197 441 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3695 276 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI