(Circular) (Six-base) MAPSORT of: pFN21AB5199.seq Check: 1989 from: 1 to: 6061 pFN21AB5199 6061 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2739 2739 Size: 6061 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2940 3871 278 Size: 53 83 186 936 1404 931 2468 Fragments arranged by size: 2468 1404 936 931 186 83 53 AccI GT'mk_AC Cuts at: 1937 2583 2940 3404 1937 Size: 646 357 464 4594 Fragments arranged by size: 4594 646 464 357 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4124 5508 5776 1632 Size: 2492 1384 268 1917 Fragments arranged by size: 2492 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 4960 5937 1203 Size: 3757 977 1327 Fragments arranged by size: 3757 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 2861 1166 Size: 1695 4366 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2675 2707 2675 Size: 32 6029 AlwNI CAG_nnn'CTG Cuts at: 3124 3804 5376 3124 Size: 680 1572 3809 Fragments arranged by size: 3809 1572 680 ApaLI G'TGCA_C Cuts at: 4118 5274 4118 Size: 1156 4905 ApoI r'AATT_y Cuts at: 1493 3370 3596 3748 5857 1493 Size: 1877 226 152 2109 1697 Fragments arranged by size: 2109 1877 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 3154 3386 1870 Size: 73 1211 232 4545 Fragments arranged by size: 4545 1211 232 73 BamHI G'GATC_C Cuts at: 3391 3758 3391 Size: 367 5694 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2830 3382 4837 618 Size: 325 217 627 279 764 552 1455 1842 Fragments arranged by size: 1842 1455 764 627 552 325 279 217 BanII G_rGCy'C Cuts at: 1391 2105 3173 3380 1391 Size: 714 1068 207 4072 Fragments arranged by size: 4072 1068 714 207 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2953 928 Size: 971 1054 4036 Fragments arranged by size: 4036 1054 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4252 4286 1903 Size: 34 2315 34 3678 Fragments arranged by size: 3678 2315 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2020 2396 2927 Size: 107 122 71 631 605 348 376 531 Cuts at: 2927 3243 4672 136 Size: 316 1429 1525 Fragments arranged by size: 1525 1429 631 605 531 376 348 316 122 107 71 BglII A'GATC_T Cuts at: 2115 6056 2115 Size: 3941 2120 BlpI GC'TnA_GC Cuts at: 2714 3478 2714 Size: 764 5297 Bme1580I G_kGCm'C Cuts at: 1790 2835 2896 3256 4122 5278 1790 Size: 1045 61 360 866 1156 2573 Fragments arranged by size: 2573 1156 1045 866 360 61 BmgBI CAC'GTC Cuts at: 1318 2413 3070 1318 Size: 1095 657 4309 Fragments arranged by size: 4309 1095 657 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3352 3792 4750 Size: 416 53 83 399 24 1935 440 958 Cuts at: 4750 442 Size: 1753 Fragments arranged by size: 1935 1753 958 440 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2234 2570 3031 4721 1239 Size: 893 102 336 461 1690 2579 Fragments arranged by size: 2579 1690 893 461 336 102 Bpu10I CC'TnA_GC Cuts at: 3229 3260 3229 Size: 31 6030 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4186 5051 5349 4186 Size: 865 298 4898 Fragments arranged by size: 4898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2318 2809 3049 4724 882 Size: 617 819 491 240 1675 2219 Fragments arranged by size: 2219 1675 819 617 491 240 BsaAI yAC'GTr Cuts at: 493 1204 2098 3852 493 Size: 711 894 1754 2702 Fragments arranged by size: 2702 1754 894 711 BsaBI GATnn'nnATC Cuts at: 6055 6055 Size: 6061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2937 3111 3868 4250 Size: 53 83 186 936 1404 174 757 382 Cuts at: 4250 4885 275 Size: 635 1451 Fragments arranged by size: 1451 1404 936 757 635 382 186 174 83 53 BsaWI w'CCGG_w Cuts at: 4494 4892 5166 5313 5724 4494 Size: 398 274 147 411 4831 Fragments arranged by size: 4831 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2087 2117 2759 2789 1178 Size: 30 165 30 684 30 642 30 4450 Fragments arranged by size: 4450 684 642 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2890 1186 Size: 847 857 4357 Fragments arranged by size: 4357 857 847 BseYI C'CCAG_C Cuts at: 5264 5760 5264 Size: 496 5565 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2261 2705 1163 Size: 573 525 444 4519 Fragments arranged by size: 4519 573 525 444 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3327 3511 4272 5300 1540 Size: 275 186 1326 184 761 1028 2301 Fragments arranged by size: 2301 1326 1028 761 275 186 184 BsiHKAI G_wGCw'C Cuts at: 2610 4122 4207 5278 2610 Size: 1512 85 1071 3393 Fragments arranged by size: 3393 1512 1071 85 BsmI GAATG_Cn' Cuts at: 3572 3665 3572 Size: 93 5968 BsmBI CGTCTCn'nnnn_ Cuts at: 3197 3197 Size: 6061 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2105 2610 2835 2896 3173 3256 3380 Size: 399 315 505 225 61 277 83 124 Cuts at: 3380 4122 4207 5278 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 505 399 315 277 225 124 85 83 61 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 3950 5680 3950 Size: 1730 4331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2739 3416 844 Size: 1895 677 3489 Fragments arranged by size: 3489 1895 677 BsrBI CCG'CTC Cuts at: 1394 1823 2035 2898 3228 3948 1394 Size: 429 212 863 330 720 3507 Fragments arranged by size: 3507 863 720 429 330 212 BsrDI GCAATG_nn' Cuts at: 65 1248 4556 4730 65 Size: 1183 3308 174 1396 Fragments arranged by size: 3308 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2150 3107 3138 4705 4892 1700 Size: 110 340 957 31 1567 187 2869 Fragments arranged by size: 2869 1567 957 340 187 110 31 BsrGI T'GTAC_A Cuts at: 96 2463 2751 96 Size: 2367 288 3406 Fragments arranged by size: 3406 2367 288 BssHII G'CGCG_C Cuts at: 1925 2049 1925 Size: 124 5937 BssSI C'ACGA_G Cuts at: 4121 5133 4121 Size: 1012 5049 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6061 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstXI CCAn_nnnn'nTGG Cuts at: 2884 2884 Size: 6061 BstYI r'GATC_y Cuts at: 1972 2115 2949 3391 3758 4143 4160 5612 5698 Size: 143 834 442 367 385 17 1452 86 Cuts at: 5698 5710 6056 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 834 442 385 367 346 143 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2062 2520 2614 2670 2708 2743 Size: 552 939 58 458 94 56 38 35 Cuts at: 2743 2859 2909 3357 513 Size: 116 50 448 3217 Fragments arranged by size: 3217 939 552 458 448 116 94 58 56 50 38 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2090 487 Size: 683 56 864 4458 Fragments arranged by size: 4458 864 683 56 BtsI GCAGTG_nn' Cuts at: 3577 4371 4391 3577 Size: 794 20 5247 Fragments arranged by size: 5247 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DraIII CAC_nnn'GTG Cuts at: 2413 2413 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2220 2508 2698 Size: 54 1425 50 275 18 390 288 190 Cuts at: 2698 3126 3324 3508 4397 6026 8 Size: 428 198 184 889 1629 43 Fragments arranged by size: 1629 1425 889 428 390 288 275 198 190 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3324 3508 1537 Size: 275 1512 184 4090 Fragments arranged by size: 4090 1512 275 184 EarI CTCTTCn'nnn_ Cuts at: 1353 3095 3991 1353 Size: 1742 896 3423 Fragments arranged by size: 3423 1742 896 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4648 5022 5168 387 Size: 1064 138 3059 374 146 1280 Fragments arranged by size: 3059 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2234 2570 3031 4124 4721 5508 Size: 393 500 102 336 461 1093 597 787 Cuts at: 5508 5776 1239 Size: 268 1524 Fragments arranged by size: 1524 1093 787 597 500 461 393 336 268 102 EcoO109I rG'GnC_Cy Cuts at: 2186 2186 Size: 6061 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 3148 5208 1678 Size: 1470 2060 2531 Fragments arranged by size: 2531 2060 1470 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2222 2254 4702 4734 4776 Size: 32 135 32 650 32 2448 32 42 Cuts at: 4776 4808 1373 Size: 32 2626 Fragments arranged by size: 2626 2448 650 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 2574 5175 5359 1305 Size: 825 444 2601 184 2007 Fragments arranged by size: 2601 2007 825 444 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2222 2510 2700 3128 10 Size: 54 1425 343 390 288 190 428 2943 Fragments arranged by size: 2943 1425 428 390 343 288 190 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 2861 2882 4019 4378 4537 518 Size: 648 1101 594 21 1137 359 159 2042 Fragments arranged by size: 2042 1137 1101 648 594 359 159 21 MspA1I CmG'CkG Cuts at: 1606 2064 2635 2696 2745 3477 4154 5302 5547 Size: 458 571 61 49 732 677 1148 245 Cuts at: 5547 1606 Size: 2120 Fragments arranged by size: 2120 1148 732 677 571 458 245 61 49 NaeI GCC'GGC Cuts at: 2152 3109 3140 2152 Size: 957 31 5073 Fragments arranged by size: 5073 957 31 NcoI C'CATG_G Cuts at: 513 1065 2004 2520 3357 513 Size: 552 939 516 837 3217 Fragments arranged by size: 3217 939 837 552 516 NdeI CA'TA_TG Cuts at: 387 387 Size: 6061 NgoMIV G'CCGG_C Cuts at: 2150 3107 3138 2150 Size: 957 31 5073 Fragments arranged by size: 5073 957 31 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 2542 2773 3419 4964 1132 Size: 97 1313 231 646 1545 2229 Fragments arranged by size: 2229 1545 1313 646 231 97 PasI CC'CwG_GG Cuts at: 1348 2329 1348 Size: 981 5080 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 2620 2799 1108 Size: 1512 179 4370 Fragments arranged by size: 4370 1512 179 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PstI C_TGCA'G Cuts at: 830 2445 2634 3413 830 Size: 1615 189 779 3478 Fragments arranged by size: 3478 1615 779 189 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 PvuII CAG'CTG Cuts at: 2635 2635 Size: 6061 SacII CC_GC'GG Cuts at: 2065 2746 2065 Size: 681 5380 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SfcI C'TryA_G Cuts at: 826 1045 2441 2630 3013 3175 3409 4544 5225 Size: 219 1396 189 383 162 234 1135 681 Cuts at: 5225 5416 826 Size: 191 1471 Fragments arranged by size: 1471 1396 1135 681 383 234 219 191 189 162 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 3156 3388 1872 Size: 1284 232 4545 Fragments arranged by size: 4545 1284 232 SmlI C'TyrA_G Cuts at: 820 1017 1943 4165 5066 5328 820 Size: 197 926 2222 901 262 1553 Fragments arranged by size: 2222 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 2542 3419 1132 Size: 1410 877 3774 Fragments arranged by size: 3774 1410 877 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6061 StyI C'CwwG_G Cuts at: 513 1065 2004 2520 3033 3357 3500 513 Size: 552 939 516 513 324 143 3074 Fragments arranged by size: 3074 939 552 516 513 324 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4088 4105 4258 4443 4888 325 Size: 1255 2508 17 153 185 445 1498 Fragments arranged by size: 2508 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2256 2463 2751 Size: 274 80 33 51 1443 279 207 288 Cuts at: 2751 4307 6019 96 Size: 1556 1712 138 Fragments arranged by size: 1712 1556 1443 288 279 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2345 2377 6021 6053 2345 Size: 32 3644 32 2353 Fragments arranged by size: 3644 2353 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2579 1316 Size: 1263 4798 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2326 2518 2929 2326 Size: 192 411 5458 Fragments arranged by size: 5458 411 192 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 3154 3386 1870 Size: 1284 232 4545 Fragments arranged by size: 4545 1284 232 XmnI GAAnn'nnTTC Cuts at: 4190 4190 Size: 6061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2938 3869 276 Size: 53 83 186 936 1404 931 2468 Fragments arranged by size: 2468 1404 936 931 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI