Restriction map for
pFN21AB5199
(Linear) (Six-base) MAPSORT of: KIBB5199.seq Check: 7985 from: 1 to: 1356
KIBB5199.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 734 1356
Size: 734 622
AatII G_ACGT'C
Cuts at: 0 935 1356
Size: 935 421
AccI GT'mk_AC
Cuts at: 0 578 935 1356
Size: 578 357 421
Fragments arranged by size:
578 421 357
AleI CACnn'nnGTG
Cuts at: 0 856 1356
Size: 856 500
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 670 702 1356
Size: 670 32 654
Fragments arranged by size:
670 654 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1119 1356
Size: 1119 237
AvaI C'yCGr_G
Cuts at: 0 1149 1356
Size: 1149 207
BanI G'GyrC_C
Cuts at: 0 61 825 1356
Size: 61 764 531
Fragments arranged by size:
764 531 61
BanII G_rGCy'C
Cuts at: 0 100 1168 1356
Size: 100 1068 188
Fragments arranged by size:
1068 188 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 948 1356
Size: 948 408
BglI GCCn_nnn'nGGC
Cuts at: 0 15 391 922 1238 1356
Size: 15 376 531 316 118
Fragments arranged by size:
531 376 316 118 15
BglII A'GATC_T
Cuts at: 0 110 1356
Size: 110 1246
BlpI GC'TnA_GC
Cuts at: 0 709 1356
Size: 709 647
Bme1580I G_kGCm'C
Cuts at: 0 830 891 1251 1356
Size: 830 61 360 105
Fragments arranged by size:
830 360 105 61
BmgBI CAC'GTC
Cuts at: 0 408 1065 1356
Size: 408 657 291
Fragments arranged by size:
657 408 291
BmrI ACTGGGnnnn_n'
Cuts at: 0 1347 1356
Size: 1347 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 229 565 1026 1356
Size: 127 102 336 461 330
Fragments arranged by size:
461 336 330 127 102
Bpu10I CC'TnA_GC
Cuts at: 0 1224 1255 1356
Size: 1224 31 101
Fragments arranged by size:
1224 101 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 313 804 1044 1356
Size: 313 491 240 312
Fragments arranged by size:
491 313 312 240
BsaAI yAC'GTr
Cuts at: 0 93 1356
Size: 93 1263
BsaHI Gr'CG_yC
Cuts at: 0 932 1106 1356
Size: 932 174 250
Fragments arranged by size:
932 250 174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 754 784 1356
Size: 82 30 642 30 572
Fragments arranged by size:
642 572 82 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 885 1356
Size: 28 857 471
Fragments arranged by size:
857 471 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 700 1356
Size: 256 444 656
Fragments arranged by size:
656 444 256
BsiEI CG_ry'CG
Cuts at: 0 1322 1356
Size: 1322 34
BsiHKAI G_wGCw'C
Cuts at: 0 605 1356
Size: 605 751
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1192 1356
Size: 1192 164
Bsp1286I G_dGCh'C
Cuts at: 0 100 605 830 891 1168 1251 1356
Size: 100 505 225 61 277 83 105
Fragments arranged by size:
505 277 225 105 100 83 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 734 1356
Size: 734 622
BsrBI CCG'CTC
Cuts at: 0 30 893 1223 1356
Size: 30 863 330 133
Fragments arranged by size:
863 330 133 30
BsrFI r'CCGG_y
Cuts at: 0 145 1102 1133 1356
Size: 145 957 31 223
Fragments arranged by size:
957 223 145 31
BsrGI T'GTAC_A
Cuts at: 0 458 746 1356
Size: 458 288 610
Fragments arranged by size:
610 458 288
BssHII G'CGCG_C
Cuts at: 0 44 1356
Size: 44 1312
BstXI CCAn_nnnn'nTGG
Cuts at: 0 879 1356
Size: 879 477
BstYI r'GATC_y
Cuts at: 0 110 944 1356
Size: 110 834 412
Fragments arranged by size:
834 412 110
BtgI C'CryG_G
Cuts at: 0 57 515 609 665 703 738 854 904
Size: 57 458 94 56 38 35 116 50
Cuts at: 904 1356
Size: 452
Fragments arranged by size:
458 452 116 94 57 56 50 38
35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 85 1356
Size: 85 1271
DraIII CAC_nnn'GTG
Cuts at: 0 408 1356
Size: 408 948
EaeI y'GGCC_r
Cuts at: 0 215 503 693 1121 1319 1356
Size: 215 288 190 428 198 37
Fragments arranged by size:
428 288 215 198 190 37
EagI C'GGCC_G
Cuts at: 0 1319 1356
Size: 1319 37
EarI CTCTTCn'nnn_
Cuts at: 0 1090 1356
Size: 1090 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 229 565 1026 1356
Size: 127 102 336 461 330
Fragments arranged by size:
461 336 330 127 102
EcoO109I rG'GnC_Cy
Cuts at: 0 181 1356
Size: 181 1175
HaeII r_GCGC'y
Cuts at: 0 1143 1356
Size: 1143 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 1356
Size: 217 32 1107
Fragments arranged by size:
1107 217 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 569 1356
Size: 125 444 787
Fragments arranged by size:
787 444 125
MscI TGG'CCA
Cuts at: 0 217 505 695 1123 1356
Size: 217 288 190 428 233
Fragments arranged by size:
428 288 233 217 190
MslI CAynn'nnrTG
Cuts at: 0 262 856 877 1356
Size: 262 594 21 479
Fragments arranged by size:
594 479 262 21
MspA1I CmG'CkG
Cuts at: 0 59 630 691 740 1356
Size: 59 571 61 49 616
Fragments arranged by size:
616 571 61 59 49
NaeI GCC'GGC
Cuts at: 0 147 1104 1135 1356
Size: 147 957 31 221
Fragments arranged by size:
957 221 147 31
NcoI C'CATG_G
Cuts at: 0 515 1356
Size: 515 841
NgoMIV G'CCGG_C
Cuts at: 0 145 1102 1133 1356
Size: 145 957 31 223
Fragments arranged by size:
957 223 145 31
NspI r_CATG'y
Cuts at: 0 537 768 1356
Size: 537 231 588
Fragments arranged by size:
588 537 231
PasI CC'CwG_GG
Cuts at: 0 324 1356
Size: 324 1032
PflMI CCAn_nnn'nTGG
Cuts at: 0 615 794 1356
Size: 615 179 562
Fragments arranged by size:
615 562 179
PstI C_TGCA'G
Cuts at: 0 440 629 1356
Size: 440 189 727
Fragments arranged by size:
727 440 189
PvuII CAG'CTG
Cuts at: 0 630 1356
Size: 630 726
SacII CC_GC'GG
Cuts at: 0 60 741 1356
Size: 60 681 615
Fragments arranged by size:
681 615 60
SfcI C'TryA_G
Cuts at: 0 436 625 1008 1170 1356
Size: 436 189 383 162 186
Fragments arranged by size:
436 383 189 186 162
SmaI CCC'GGG
Cuts at: 0 1151 1356
Size: 1151 205
SphI G_CATG'C
Cuts at: 0 537 1356
Size: 537 819
StyI C'CwwG_G
Cuts at: 0 515 1028 1356
Size: 515 513 328
Fragments arranged by size:
515 513 328
TatI w'GTAC_w
Cuts at: 0 251 458 746 1356
Size: 251 207 288 610
Fragments arranged by size:
610 288 251 207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 340 372 1356
Size: 340 32 984
Fragments arranged by size:
984 340 32
Tth111I GACn'n_nGTC
Cuts at: 0 574 1356
Size: 574 782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 321 513 924 1356
Size: 321 192 411 432
Fragments arranged by size:
432 411 321 192
XmaI C'CCGG_G
Cuts at: 0 1149 1356
Size: 1149 207
ZraI GAC'GTC
Cuts at: 0 933 1356
Size: 933 423
Enzymes that do cut:
AarI AatII AccI AleI AloI AlwNI AvaI BanI
BanII BbsI BglI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI
BssHII BstXI BstYI BtgI BtgZI DraIII EaeI EagI
EarI Eco57MI EcoO109I HaeII Hin4I MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PstI
PvuII SacII SfcI SmaI SphI StyI TatI TstI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BmtI BplI BpuEI BsaBI BsaWI BseYI BsiWI BsmI
BspEI BspHI BsrDI BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtsI ClaI CspCI DraI DrdI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TaqII XbaI XhoI XmnI