(Circular) (Six-base) MAPSORT of: pFN21AB5162.seq Check: 7396 from: 1 to: 5356 pFN21AB5162 5356 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3166 278 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2699 1937 Size: 762 4594 Acc65I G'GTAC_C Cuts at: 2677 2677 Size: 5356 AclI AA'CG_TT Cuts at: 1563 3483 3856 1563 Size: 1920 373 3063 Fragments arranged by size: 3063 1920 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3419 4803 5071 1632 Size: 1787 1384 268 1917 Fragments arranged by size: 1917 1787 1384 268 AfeI AGC'GCT Cuts at: 2275 2275 Size: 5356 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5159 AflIII A'CryG_T Cuts at: 1203 4255 5232 1203 Size: 3052 977 1327 Fragments arranged by size: 3052 1327 977 AgeI A'CCGG_T Cuts at: 4187 4187 Size: 5356 AhdI GACnn_n'nnGTC Cuts at: 4085 4085 Size: 5356 AleI CACnn'nnGTG Cuts at: 1166 2179 2639 1166 Size: 1013 460 3883 Fragments arranged by size: 3883 1013 460 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5356 AlwNI CAG_nnn'CTG Cuts at: 3099 4671 3099 Size: 1572 3784 ApaLI G'TGCA_C Cuts at: 3413 4569 3413 Size: 1156 4200 ApoI r'AATT_y Cuts at: 1493 2665 2891 3043 5152 1493 Size: 1172 226 152 2109 1697 Fragments arranged by size: 2109 1697 1172 226 152 AseI AT'TA_AT Cuts at: 160 1032 3910 160 Size: 872 2878 1606 Fragments arranged by size: 2878 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 AvaI C'yCGr_G Cuts at: 1870 1943 2681 1870 Size: 73 738 4545 Fragments arranged by size: 4545 738 73 BamHI G'GATC_C Cuts at: 2686 3053 2686 Size: 367 4989 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2464 2677 4132 618 Size: 325 217 627 677 213 1455 1842 Fragments arranged by size: 1842 1455 677 627 325 217 213 BanII G_rGCy'C Cuts at: 1391 2675 1391 Size: 1284 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4385 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3547 3581 1903 Size: 34 1610 34 3678 Fragments arranged by size: 3678 1610 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3248 4458 3248 Size: 1210 4146 BclI T'GATC_A Cuts at: 1548 2101 1548 Size: 553 4803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3967 136 Size: 107 122 71 631 605 2295 1525 Fragments arranged by size: 2295 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2411 5351 2411 Size: 2940 2416 BlpI GC'TnA_GC Cuts at: 2054 2225 2773 2054 Size: 171 548 4637 Fragments arranged by size: 4637 548 171 Bme1580I G_kGCm'C Cuts at: 1790 3417 4573 1790 Size: 1627 1156 2573 Fragments arranged by size: 2573 1627 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5356 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3087 4045 442 Size: 416 53 83 399 24 1670 958 1753 Fragments arranged by size: 1753 1670 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2410 4016 1239 Size: 1171 1606 2579 Fragments arranged by size: 2579 1606 1171 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2063 3481 4346 4644 2063 Size: 1418 865 298 2775 Fragments arranged by size: 2775 1418 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4019 882 Size: 617 2520 2219 Fragments arranged by size: 2520 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3147 493 Size: 711 1943 2702 Fragments arranged by size: 2702 1943 711 BsaBI GATnn'nnATC Cuts at: 2416 5350 2416 Size: 2934 2422 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3163 3545 4180 275 Size: 53 83 186 936 1630 382 635 1451 Fragments arranged by size: 1630 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2646 3789 4187 4461 4608 5019 2646 Size: 1143 398 274 147 411 2983 Fragments arranged by size: 2983 1143 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5131 Fragments arranged by size: 5131 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5356 BseYI C'CCAG_C Cuts at: 2527 4559 5055 2527 Size: 2032 496 2828 Fragments arranged by size: 2828 2032 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2339 1163 Size: 573 603 4180 Fragments arranged by size: 4180 603 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2806 3567 4595 1540 Size: 275 186 805 761 1028 2301 Fragments arranged by size: 2301 1028 805 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2337 3417 3502 4573 2337 Size: 1080 85 1071 3120 Fragments arranged by size: 3120 1080 1071 85 BsmI GAATG_Cn' Cuts at: 2867 2960 2867 Size: 93 5263 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2337 2675 3417 3502 4573 1391 Size: 399 547 338 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 547 399 338 85 BspEI T'CCGG_A Cuts at: 5019 5019 Size: 5356 BspHI T'CATG_A Cuts at: 3245 4975 3245 Size: 1730 3626 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2711 844 Size: 1867 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3243 1394 Size: 429 1420 3507 Fragments arranged by size: 3507 1420 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3851 4025 65 Size: 1183 2603 174 1396 Fragments arranged by size: 2603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2609 4000 4187 1700 Size: 110 799 1391 187 2869 Fragments arranged by size: 2869 1391 799 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5356 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5356 BssSI C'ACGA_G Cuts at: 3416 4428 3416 Size: 1012 4344 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5356 BstBI TT'CG_AA Cuts at: 4160 4160 Size: 5356 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5356 BstXI CCAn_nnnn'nTGG Cuts at: 2181 2519 2181 Size: 338 5018 BstYI r'GATC_y Cuts at: 1972 2411 2686 3053 3438 3455 4907 4993 5005 Size: 439 275 367 385 17 1452 86 12 Cuts at: 5005 5351 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 439 385 367 346 275 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2180 2599 513 Size: 552 939 176 419 3270 Fragments arranged by size: 3270 939 552 419 176 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 1999 487 Size: 683 56 773 3844 Fragments arranged by size: 3844 773 683 56 BtsI GCAGTG_nn' Cuts at: 2872 3666 3686 2872 Size: 794 20 4542 Fragments arranged by size: 4542 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5356 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5321 DraI TTT'AAA Cuts at: 2660 2660 Size: 5356 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4363 809 Size: 507 3047 1802 Fragments arranged by size: 3047 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2803 3692 5321 Size: 54 1425 50 275 18 973 889 1629 Cuts at: 5321 8 Size: 43 Fragments arranged by size: 1629 1425 973 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2803 1537 Size: 275 991 4090 Fragments arranged by size: 4090 991 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3286 1353 Size: 1933 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2525 3943 4317 4463 387 Size: 1064 138 936 1418 374 146 1280 Fragments arranged by size: 1418 1280 1064 936 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2410 3419 4016 4803 5071 1239 Size: 393 778 1009 597 787 268 1524 Fragments arranged by size: 1524 1009 787 778 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2247 2491 2247 Size: 244 5112 EcoRI G'AATT_C Cuts at: 2665 3043 2665 Size: 378 4978 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2240 2240 Size: 5356 FspI TGC'GCA Cuts at: 3862 3862 Size: 5356 HaeII r_GCGC'y Cuts at: 1678 2277 4503 1678 Size: 599 2226 2531 Fragments arranged by size: 2531 2226 599 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2535 2567 3997 4029 4071 Size: 32 135 32 963 32 1430 32 42 Cuts at: 4071 4103 1373 Size: 32 2626 Fragments arranged by size: 2626 1430 963 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2700 2946 669 Size: 988 281 762 246 3079 Fragments arranged by size: 3079 988 762 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5356 HpaI GTT'AAC Cuts at: 2946 2946 Size: 5356 KpnI G_GTAC'C Cuts at: 2681 2681 Size: 5356 MfeI C'AATT_G Cuts at: 2955 2955 Size: 5356 MluI A'CGCG_T Cuts at: 5232 5232 Size: 5356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4470 4654 1305 Size: 3165 184 2007 Fragments arranged by size: 3165 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3534 Fragments arranged by size: 3534 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2165 2179 2639 3314 3673 3832 518 Size: 648 999 14 460 675 359 159 2042 Fragments arranged by size: 2042 999 675 648 460 359 159 14 MspA1I CmG'CkG Cuts at: 1606 2236 2772 3449 4597 4842 1606 Size: 630 536 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 630 536 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3865 Fragments arranged by size: 3865 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5356 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5356 NotI GC'GGCC_GC Cuts at: 2803 2803 Size: 5356 NspI r_CATG'y Cuts at: 1132 1229 2617 2714 4259 1132 Size: 97 1388 97 1545 2229 Fragments arranged by size: 2229 1545 1388 97 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5356 PciI A'CATG_T Cuts at: 4255 4255 Size: 5356 PflMI CCAn_nnn'nTGG Cuts at: 1108 2560 2583 1108 Size: 1452 23 3881 Fragments arranged by size: 3881 1452 23 PmeI GTTT'AAAC Cuts at: 2660 2660 Size: 5356 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3425 3457 4938 4970 3425 Size: 32 1481 32 3811 Fragments arranged by size: 3811 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2483 1613 Size: 870 4486 PsiI TTA'TAA Cuts at: 2926 2926 Size: 5356 PstI C_TGCA'G Cuts at: 830 2044 2708 830 Size: 1214 664 3478 Fragments arranged by size: 3478 1214 664 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5356 SalI G'TCGA_C Cuts at: 1936 2698 1936 Size: 762 4594 SbfI CC_TGCA'GG Cuts at: 2708 2708 Size: 5356 ScaI AGT'ACT Cuts at: 2357 3604 5316 2357 Size: 1247 1712 2397 Fragments arranged by size: 2397 1712 1247 SfcI C'TryA_G Cuts at: 826 1045 2040 2704 3839 4520 4711 826 Size: 219 995 664 1135 681 191 1471 Fragments arranged by size: 1471 1135 995 681 664 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5356 SmaI CCC'GGG Cuts at: 1872 2683 1872 Size: 811 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2078 3460 4361 4623 820 Size: 197 926 135 1382 901 262 1553 Fragments arranged by size: 1553 1382 926 901 262 197 135 SnaBI TAC'GTA Cuts at: 493 3147 493 Size: 2654 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5356 SphI G_CATG'C Cuts at: 1132 2714 1132 Size: 1582 3774 SspI AAT'ATT Cuts at: 5 52 3280 5 Size: 47 3228 2081 Fragments arranged by size: 3228 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5356 StyI C'CwwG_G Cuts at: 513 1065 2004 2208 2795 513 Size: 552 939 204 587 3074 Fragments arranged by size: 3074 939 587 552 204 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2356 3383 3400 3553 3738 4183 325 Size: 1255 776 1027 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1027 776 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2355 3602 5314 Size: 274 80 33 51 1443 378 1247 1712 Cuts at: 5314 96 Size: 138 Fragments arranged by size: 1712 1443 1247 378 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2301 2333 5316 5348 2301 Size: 32 2983 32 2309 Fragments arranged by size: 2983 2309 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5356 XbaI T'CTAG_A Cuts at: 2692 2692 Size: 5356 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5356 XmaI C'CCGG_G Cuts at: 1870 2681 1870 Size: 811 4545 XmnI GAAnn'nnTTC Cuts at: 2357 3485 2357 Size: 1128 4228 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3164 276 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI