Restriction map for pFN21AB5110
 (Circular) (Six-base) MAPSORT of: pFN21AB5110.seq  Check: 6453  from: 1  to: 6619



pFN21AB5110  6619 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:34  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4429     278 
   Size:         53      83     186     936    2893    2468

  Fragments arranged by size: 

                2893    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2241    3213    3962    1937 
   Size:        304     972     749    4594

  Fragments arranged by size: 

                4594     972     749     304
 
Acc65I G'GTAC_C

Cuts at:   3940    3940 
   Size:       6619
 
AclI AA'CG_TT

Cuts at:   1563    4746    5119    1563 
   Size:       3183     373    3063

  Fragments arranged by size: 

                3183    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2858    2996    3328    4682    6066    6334    1632 
   Size:       1226     138     332    1354    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1354    1226     332     268     138
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6422
 
AflIII A'CryG_T

Cuts at:   1203    2094    5518    6495    1203 
   Size:        891    3424     977    1327

  Fragments arranged by size: 

                3424    1327     977     891
 
AgeI A'CCGG_T

Cuts at:   5450    5450 
   Size:       6619
 
AhdI GACnn_n'nnGTC

Cuts at:   5348    5348 
   Size:       6619
 
AleI CACnn'nnGTG

Cuts at:   1166    3461    1166 
   Size:       2295    4324
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3479    3603    1184 
   Size:       2295     124    4200

  Fragments arranged by size: 

                4200    2295     124
 
AlwNI CAG_nnn'CTG

Cuts at:   2567    2881    3193    4362    5934    2567 
   Size:        314     312    1169    1572    3252

  Fragments arranged by size: 

                3252    1572    1169     314     312
 
ApaLI G'TGCA_C

Cuts at:   4676    5832    4676 
   Size:       1156    5463
 
ApoI r'AATT_y

Cuts at:   1493    2417    3770    3928    4154    4306    6415    1493 
   Size:        924    1353     158     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1353     924     226     158     152
 
AseI AT'TA_AT

Cuts at:    160    1032    5173     160 
   Size:        872    4141    1606

  Fragments arranged by size: 

                4141    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6619
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2739    3944    1870 
   Size:         73     796    1205    4545

  Fragments arranged by size: 

                4545    1205     796      73
 
BamHI G'GATC_C

Cuts at:   3949    4316    3949 
   Size:        367    6252
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3848    3940    5395     618 
   Size:        325     217     627    2061      92    1455    1842

  Fragments arranged by size: 

                2061    1842    1455     627     325     217      92
 
BanII G_rGCy'C

Cuts at:   1391    3938    1391 
   Size:       2547    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2576    3018     928 
   Size:        971     677     442    4529

  Fragments arranged by size: 

                4529     971     677     442
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    2894    2928    3817    3851    4810 
   Size:         34      50      34     873      34     889      34     959

Cuts at:   4810    4844    1903 
   Size:         34    3678

  Fragments arranged by size: 

                3678     959     889     873      50      34      34      34
                  34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4511    5721    4511 
   Size:       1210    5409
 
BclI T'GATC_A

Cuts at:   1548    3043    1548 
   Size:       1495    5124
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5230     136 
   Size:        107     122      71     631     605    3558    1525

  Fragments arranged by size: 

                3558    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6614    6614 
   Size:       6619
 
BlpI GC'TnA_GC

Cuts at:   4036    4036 
   Size:       6619
 
Bme1580I G_kGCm'C

Cuts at:   1790    3427    3851    4680    5836    1790 
   Size:       1637     424     829    1156    2573

  Fragments arranged by size: 

                2573    1637    1156     829     424
 
BmgBI CAC'GTC

Cuts at:   1318    2260    3550    1318 
   Size:        942    1290    4387

  Fragments arranged by size: 

                4387    1290     942
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2762    2865    2963 
   Size:        416      53      83     399      24    1345     103      98

Cuts at:   2963    4350    5308     442 
   Size:       1387     958    1753

  Fragments arranged by size: 

                1753    1387    1345     958     416     399     103      98
                  83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6619
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3420    5279    1239 
   Size:       2181    1859    2579

  Fragments arranged by size: 

                2579    2181    1859
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2150    3022    4744    5609    5907    2150 
   Size:        872    1722     865     298    2862

  Fragments arranged by size: 

                2862    1722     872     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5282     882 
   Size:        617    3783    2219

  Fragments arranged by size: 

                3783    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3533    4410     493 
   Size:        711    2329     877    2702

  Fragments arranged by size: 

                2702    2329     877     711
 
BsaBI GATnn'nnATC

Cuts at:   6613    6613 
   Size:       6619
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4426    4808    5443     275 
   Size:         53      83     186     936    2893     382     635    1451

  Fragments arranged by size: 

                2893    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2081    5052    5450    5724    5871    6282    2081 
   Size:       2971     398     274     147     411    2418

  Fragments arranged by size: 

                2971    2418     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    6394

  Fragments arranged by size: 

                6394     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2393    2639    2663    1186 
   Size:       1207     246      24    5142

  Fragments arranged by size: 

                5142    1207     246      24
 
BseYI C'CCAG_C

Cuts at:   3240    3380    5822    6318    3240 
   Size:        140    2442     496    3541

  Fragments arranged by size: 

                3541    2442     496     140
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3206    3485    1163 
   Size:        573    1470     279    4297

  Fragments arranged by size: 

                4297    1470     573     279
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4069    4830    5858    1540 
   Size:        275     186    2068     761    1028    2301

  Fragments arranged by size: 

                2301    2068    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2049    2401    4680    4765    5836    2049 
   Size:        352    2279      85    1071    2832

  Fragments arranged by size: 

                2832    2279    1071     352      85
 
BsmI GAATG_Cn'

Cuts at:   4130    4223    4130 
   Size:         93    6526
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2049    2401    3427    3851    3938    4680    4765 
   Size:        399     259     352    1026     424      87     742      85

Cuts at:   4765    5836    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071    1026     742     424     399     352     259
                  87      85
 
BspEI T'CCGG_A

Cuts at:   6282    6282 
   Size:       6619
 
BspHI T'CATG_A

Cuts at:   3909    4508    6238    3909 
   Size:        599    1730    4290

  Fragments arranged by size: 

                4290    1730     599
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3974     844 
   Size:       3130    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4506    1394 
   Size:        429    2683    3507

  Fragments arranged by size: 

                3507    2683     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3051    3075    3354    3504    3519    3679    5114 
   Size:       1183    1803      24     279     150      15     160    1435

Cuts at:   5114    5288      65 
   Size:        174    1396

  Fragments arranged by size: 

                1803    1435    1396    1183     279     174     160     150
                  24      15
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3851    5263    5450    1700 
   Size:        110    2041    1412     187    2869

  Fragments arranged by size: 

                2869    2041    1412     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6619
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6619
 
BssSI C'ACGA_G

Cuts at:   4679    5691    4679 
   Size:       1012    5607
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3077    3193     794 
   Size:       2283     116    4220

  Fragments arranged by size: 

                4220    2283     116
 
BstBI TT'CG_AA

Cuts at:   5423    5423 
   Size:       6619
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6619
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3112    3895    3112 
   Size:        783    5836
 
BstYI r'GATC_y

Cuts at:   1972    2220    2295    2391    2878    3949    4316    4701    4718 
   Size:        248      75      96     487    1071     367     385      17

Cuts at:   4718    6170    6256    6268    6614    1972 
   Size:       1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452    1071     487     385     367     346     248
                  96      86      75      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2814    2814 
   Size:       6619
 
BtgI C'CryG_G

Cuts at:    513    1065    3387    3459    3510    3681     513 
   Size:        552    2322      72      51     171    3451

  Fragments arranged by size: 

                3451    2322     552     171      72      51
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5880

  Fragments arranged by size: 

                5880     683      56
 
BtsI GCAGTG_nn'

Cuts at:   4135    4929    4949    4135 
   Size:        794      20    5805

  Fragments arranged by size: 

                5805     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6619
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6584
 
DraI TTT'AAA

Cuts at:   3923    3923 
   Size:       6619
 
DraIII CAC_nnn'GTG

Cuts at:   2957    2957 
   Size:       6619
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3080    3692    5626     809 
   Size:        507    1764     612    1934    1802

  Fragments arranged by size: 

                1934    1802    1764     612     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3451    3609    3853 
   Size:         54    1425      50     275      18    1621     158     244

Cuts at:   3853    4066    4955    6584       8 
   Size:        213     889    1629      43

  Fragments arranged by size: 

                1629    1621    1425     889     275     244     213     158
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4066    1537 
   Size:        275    2254    4090

  Fragments arranged by size: 

                4090    2254     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    4549    1353 
   Size:       3196    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5206    5580    5726     387 
   Size:       1064     138    3617     374     146    1280

  Fragments arranged by size: 

                3617    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2858    2996    3328    3420    4682    5279    6066 
   Size:        393    1226     138     332      92    1262     597     787

Cuts at:   6066    6334    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1262    1226     787     597     393     332     268
                 138      92
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3911    3911 
   Size:       6619
 
EcoO109I rG'GnC_Cy

Cuts at:   2594    2980    2594 
   Size:        386    6233
 
EcoRI G'AATT_C

Cuts at:   3928    4306    3928 
   Size:        378    6241
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3337    3337 
   Size:       6619
 
FspI TGC'GCA

Cuts at:   5125    5125 
   Size:       6619
 
HaeII r_GCGC'y

Cuts at:   1678    3256    5766    1678 
   Size:       1578    2510    2531

  Fragments arranged by size: 

                2531    2510    1578
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2383    2415    2989    3005    3021 
   Size:         32     135      32     811      32     574      16      16

Cuts at:   3021    3028    3037    3060    5260    5292    5334    5366    1373 
   Size:          7       9      23    2200      32      42      32    2626

  Fragments arranged by size: 

                2626    2200     811     574     135      42      32      32
                  32      32      32      23      16      16       9       7
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2092    3963    4209     669 
   Size:        988     281     154    1871     246    3079

  Fragments arranged by size: 

                3079    1871     988     281     246     154
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6619
 
HpaI GTT'AAC

Cuts at:   2092    4209    2092 
   Size:       2117    4502
 
KpnI G_GTAC'C

Cuts at:   3944    3944 
   Size:       6619
 
MfeI C'AATT_G

Cuts at:   4218    4218 
   Size:       6619
 
MluI A'CGCG_T

Cuts at:   2094    6495    2094 
   Size:       4401    2218
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2323    2712    2760    3093    5733    5917    1305 
   Size:       1018     389      48     333    2640     184    2007

  Fragments arranged by size: 

                2640    2007    1018     389     333     184      48
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    3611      10 
   Size:         54    1425     343    1779    3018

  Fragments arranged by size: 

                3018    1779    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2408    3461    3509    4577    4936    5095     518 
   Size:        648    1242    1053      48    1068     359     159    2042

  Fragments arranged by size: 

                2042    1242    1068    1053     648     359     159      48
            
 
MspA1I CmG'CkG

Cuts at:   1606    3397    3691    4035    4712    5860    6105    1606 
   Size:       1791     294     344     677    1148     245    2120

  Fragments arranged by size: 

                2120    1791    1148     677     344     294     245
 
NcoI C'CATG_G

Cuts at:    513    1065    3459    3510     513 
   Size:        552    2394      51    3622

  Fragments arranged by size: 

                3622    2394     552      51
 
NdeI CA'TA_TG

Cuts at:    387    2236     387 
   Size:       1849    4770
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6619
 
NotI GC'GGCC_GC

Cuts at:   4066    4066 
   Size:       6619
 
NspI r_CATG'y

Cuts at:   1132    1229    2965    3220    3580    3977    5522    1132 
   Size:         97    1736     255     360     397    1545    2229

  Fragments arranged by size: 

                2229    1736    1545     397     360     255      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6619
 
PciI A'CATG_T

Cuts at:   5518    5518 
   Size:       6619
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3727    1108 
   Size:       2619    4000
 
PfoI T'CCnGG_A

Cuts at:   3222    3222 
   Size:       6619
 
PmeI GTTT'AAAC

Cuts at:   3923    3923 
   Size:       6619
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4688    4720    6201    6233    4688 
   Size:         32    1481      32    5074

  Fragments arranged by size: 

                5074    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2594    2594 
   Size:       6619
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6619
 
PsiI TTA'TAA

Cuts at:   4189    4189 
   Size:       6619
 
PstI C_TGCA'G

Cuts at:    830    2121    2572    2891    3073    3198    3971     830 
   Size:       1291     451     319     182     125     773    3478

  Fragments arranged by size: 

                3478    1291     773     451     319     182     125
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6619
 
PvuII CAG'CTG

Cuts at:   3397    3691    3397 
   Size:        294    6325
 
SalI G'TCGA_C

Cuts at:   1936    3961    1936 
   Size:       2025    4594
 
SbfI CC_TGCA'GG

Cuts at:   3971    3971 
   Size:       6619
 
ScaI AGT'ACT

Cuts at:   2791    4867    6579    2791 
   Size:       2076    1712    2831

  Fragments arranged by size: 

                2831    2076    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    2117    2568    2887    3069    3170    3194    3967 
   Size:        219    1072     451     319     182     101      24     773

Cuts at:   3967    5102    5783    5974     826 
   Size:       1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1072     773     681     451     319     219
                 191     182     101      24
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6619
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6619
 
SmaI CCC'GGG

Cuts at:   1872    2741    3946    1872 
   Size:        869    1205    4545

  Fragments arranged by size: 

                4545    1205     869
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2165    3001    4723    5624    5886     820 
   Size:        197     926     222     836    1722     901     262    1553

  Fragments arranged by size: 

                1722    1553     926     901     836     262     222     197
            
 
SnaBI TAC'GTA

Cuts at:    493    4410     493 
   Size:       3917    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6619
 
SphI G_CATG'C

Cuts at:   1132    3580    3977    1132 
   Size:       2448     397    3774

  Fragments arranged by size: 

                3774    2448     397
 
SspI AAT'ATT

Cuts at:      5      52    4543       5 
   Size:         47    4491    2081

  Fragments arranged by size: 

                4491    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6619
 
StyI C'CwwG_G

Cuts at:    513    1065    2031    2484    2943    3459    3510    3538    3711 
   Size:        552     966     453     459     516      51      28     173

Cuts at:   3711    3888    4058     513 
   Size:        177     170    3074

  Fragments arranged by size: 

                3074     966     552     516     459     453     177     173
                 170      51      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2162    2744    2945    4646    4663    4816    5001 
   Size:       1255     582     582     201    1701      17     153     185

Cuts at:   5001    5446     325 
   Size:        445    1498

  Fragments arranged by size: 

                1701    1498    1255     582     582     445     201     185
                 153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2789    4865    6577 
   Size:        274      80      33      51    1443     812    2076    1712

Cuts at:   6577      96 
   Size:        138

  Fragments arranged by size: 

                2076    1712    1443     812     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6579    6611    6579 
   Size:         32    6587
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2857    1316 
   Size:       1541    5078
 
XbaI T'CTAG_A

Cuts at:   3955    3955 
   Size:       6619
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2950    3841    3895    2950 
   Size:        891      54    5674

  Fragments arranged by size: 

                5674     891      54
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6619
 
XmaI C'CCGG_G

Cuts at:   1870    2739    3944    1870 
   Size:        869    1205    4545

  Fragments arranged by size: 

                4545    1205     869
 
XmnI GAAnn'nnTTC

Cuts at:   3341    4748    3341 
   Size:       1407    5212
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4427     276 
   Size:         53      83     186     936    2893    2468

  Fragments arranged by size: 

                2893    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PstI     PvuI    PvuII     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I  EcoICRI
    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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