(Circular) (Six-base) MAPSORT of: pFN21AB5110.seq Check: 6453 from: 1 to: 6619 pFN21AB5110 6619 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4429 278 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2241 3213 3962 1937 Size: 304 972 749 4594 Fragments arranged by size: 4594 972 749 304 Acc65I G'GTAC_C Cuts at: 3940 3940 Size: 6619 AclI AA'CG_TT Cuts at: 1563 4746 5119 1563 Size: 3183 373 3063 Fragments arranged by size: 3183 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2858 2996 3328 4682 6066 6334 1632 Size: 1226 138 332 1354 1384 268 1917 Fragments arranged by size: 1917 1384 1354 1226 332 268 138 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6422 AflIII A'CryG_T Cuts at: 1203 2094 5518 6495 1203 Size: 891 3424 977 1327 Fragments arranged by size: 3424 1327 977 891 AgeI A'CCGG_T Cuts at: 5450 5450 Size: 6619 AhdI GACnn_n'nnGTC Cuts at: 5348 5348 Size: 6619 AleI CACnn'nnGTG Cuts at: 1166 3461 1166 Size: 2295 4324 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3479 3603 1184 Size: 2295 124 4200 Fragments arranged by size: 4200 2295 124 AlwNI CAG_nnn'CTG Cuts at: 2567 2881 3193 4362 5934 2567 Size: 314 312 1169 1572 3252 Fragments arranged by size: 3252 1572 1169 314 312 ApaLI G'TGCA_C Cuts at: 4676 5832 4676 Size: 1156 5463 ApoI r'AATT_y Cuts at: 1493 2417 3770 3928 4154 4306 6415 1493 Size: 924 1353 158 226 152 2109 1697 Fragments arranged by size: 2109 1697 1353 924 226 158 152 AseI AT'TA_AT Cuts at: 160 1032 5173 160 Size: 872 4141 1606 Fragments arranged by size: 4141 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 AvaI C'yCGr_G Cuts at: 1870 1943 2739 3944 1870 Size: 73 796 1205 4545 Fragments arranged by size: 4545 1205 796 73 BamHI G'GATC_C Cuts at: 3949 4316 3949 Size: 367 6252 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3848 3940 5395 618 Size: 325 217 627 2061 92 1455 1842 Fragments arranged by size: 2061 1842 1455 627 325 217 92 BanII G_rGCy'C Cuts at: 1391 3938 1391 Size: 2547 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2576 3018 928 Size: 971 677 442 4529 Fragments arranged by size: 4529 971 677 442 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2894 2928 3817 3851 4810 Size: 34 50 34 873 34 889 34 959 Cuts at: 4810 4844 1903 Size: 34 3678 Fragments arranged by size: 3678 959 889 873 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4511 5721 4511 Size: 1210 5409 BclI T'GATC_A Cuts at: 1548 3043 1548 Size: 1495 5124 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5230 136 Size: 107 122 71 631 605 3558 1525 Fragments arranged by size: 3558 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6614 6614 Size: 6619 BlpI GC'TnA_GC Cuts at: 4036 4036 Size: 6619 Bme1580I G_kGCm'C Cuts at: 1790 3427 3851 4680 5836 1790 Size: 1637 424 829 1156 2573 Fragments arranged by size: 2573 1637 1156 829 424 BmgBI CAC'GTC Cuts at: 1318 2260 3550 1318 Size: 942 1290 4387 Fragments arranged by size: 4387 1290 942 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2762 2865 2963 Size: 416 53 83 399 24 1345 103 98 Cuts at: 2963 4350 5308 442 Size: 1387 958 1753 Fragments arranged by size: 1753 1387 1345 958 416 399 103 98 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6619 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3420 5279 1239 Size: 2181 1859 2579 Fragments arranged by size: 2579 2181 1859 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2150 3022 4744 5609 5907 2150 Size: 872 1722 865 298 2862 Fragments arranged by size: 2862 1722 872 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5282 882 Size: 617 3783 2219 Fragments arranged by size: 3783 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3533 4410 493 Size: 711 2329 877 2702 Fragments arranged by size: 2702 2329 877 711 BsaBI GATnn'nnATC Cuts at: 6613 6613 Size: 6619 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4426 4808 5443 275 Size: 53 83 186 936 2893 382 635 1451 Fragments arranged by size: 2893 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2081 5052 5450 5724 5871 6282 2081 Size: 2971 398 274 147 411 2418 Fragments arranged by size: 2971 2418 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6394 Fragments arranged by size: 6394 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2393 2639 2663 1186 Size: 1207 246 24 5142 Fragments arranged by size: 5142 1207 246 24 BseYI C'CCAG_C Cuts at: 3240 3380 5822 6318 3240 Size: 140 2442 496 3541 Fragments arranged by size: 3541 2442 496 140 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3206 3485 1163 Size: 573 1470 279 4297 Fragments arranged by size: 4297 1470 573 279 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4069 4830 5858 1540 Size: 275 186 2068 761 1028 2301 Fragments arranged by size: 2301 2068 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2049 2401 4680 4765 5836 2049 Size: 352 2279 85 1071 2832 Fragments arranged by size: 2832 2279 1071 352 85 BsmI GAATG_Cn' Cuts at: 4130 4223 4130 Size: 93 6526 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2401 3427 3851 3938 4680 4765 Size: 399 259 352 1026 424 87 742 85 Cuts at: 4765 5836 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1026 742 424 399 352 259 87 85 BspEI T'CCGG_A Cuts at: 6282 6282 Size: 6619 BspHI T'CATG_A Cuts at: 3909 4508 6238 3909 Size: 599 1730 4290 Fragments arranged by size: 4290 1730 599 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3974 844 Size: 3130 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4506 1394 Size: 429 2683 3507 Fragments arranged by size: 3507 2683 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3051 3075 3354 3504 3519 3679 5114 Size: 1183 1803 24 279 150 15 160 1435 Cuts at: 5114 5288 65 Size: 174 1396 Fragments arranged by size: 1803 1435 1396 1183 279 174 160 150 24 15 BsrFI r'CCGG_y Cuts at: 1700 1810 3851 5263 5450 1700 Size: 110 2041 1412 187 2869 Fragments arranged by size: 2869 2041 1412 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6619 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6619 BssSI C'ACGA_G Cuts at: 4679 5691 4679 Size: 1012 5607 BstAPI GCAn_nnn'nTGC Cuts at: 794 3077 3193 794 Size: 2283 116 4220 Fragments arranged by size: 4220 2283 116 BstBI TT'CG_AA Cuts at: 5423 5423 Size: 6619 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6619 BstXI CCAn_nnnn'nTGG Cuts at: 3112 3895 3112 Size: 783 5836 BstYI r'GATC_y Cuts at: 1972 2220 2295 2391 2878 3949 4316 4701 4718 Size: 248 75 96 487 1071 367 385 17 Cuts at: 4718 6170 6256 6268 6614 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1071 487 385 367 346 248 96 86 75 17 12 Bsu36I CC'TnA_GG Cuts at: 2814 2814 Size: 6619 BtgI C'CryG_G Cuts at: 513 1065 3387 3459 3510 3681 513 Size: 552 2322 72 51 171 3451 Fragments arranged by size: 3451 2322 552 171 72 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5880 Fragments arranged by size: 5880 683 56 BtsI GCAGTG_nn' Cuts at: 4135 4929 4949 4135 Size: 794 20 5805 Fragments arranged by size: 5805 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6619 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6584 DraI TTT'AAA Cuts at: 3923 3923 Size: 6619 DraIII CAC_nnn'GTG Cuts at: 2957 2957 Size: 6619 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3080 3692 5626 809 Size: 507 1764 612 1934 1802 Fragments arranged by size: 1934 1802 1764 612 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3451 3609 3853 Size: 54 1425 50 275 18 1621 158 244 Cuts at: 3853 4066 4955 6584 8 Size: 213 889 1629 43 Fragments arranged by size: 1629 1621 1425 889 275 244 213 158 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4066 1537 Size: 275 2254 4090 Fragments arranged by size: 4090 2254 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4549 1353 Size: 3196 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5206 5580 5726 387 Size: 1064 138 3617 374 146 1280 Fragments arranged by size: 3617 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2858 2996 3328 3420 4682 5279 6066 Size: 393 1226 138 332 92 1262 597 787 Cuts at: 6066 6334 1239 Size: 268 1524 Fragments arranged by size: 1524 1262 1226 787 597 393 332 268 138 92 EcoNI CCTnn'n_nnAGG Cuts at: 3911 3911 Size: 6619 EcoO109I rG'GnC_Cy Cuts at: 2594 2980 2594 Size: 386 6233 EcoRI G'AATT_C Cuts at: 3928 4306 3928 Size: 378 6241 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3337 3337 Size: 6619 FspI TGC'GCA Cuts at: 5125 5125 Size: 6619 HaeII r_GCGC'y Cuts at: 1678 3256 5766 1678 Size: 1578 2510 2531 Fragments arranged by size: 2531 2510 1578 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2383 2415 2989 3005 3021 Size: 32 135 32 811 32 574 16 16 Cuts at: 3021 3028 3037 3060 5260 5292 5334 5366 1373 Size: 7 9 23 2200 32 42 32 2626 Fragments arranged by size: 2626 2200 811 574 135 42 32 32 32 32 32 23 16 16 9 7 HincII GTy'rAC Cuts at: 669 1657 1938 2092 3963 4209 669 Size: 988 281 154 1871 246 3079 Fragments arranged by size: 3079 1871 988 281 246 154 HindIII A'AGCT_T Cuts at: 748 748 Size: 6619 HpaI GTT'AAC Cuts at: 2092 4209 2092 Size: 2117 4502 KpnI G_GTAC'C Cuts at: 3944 3944 Size: 6619 MfeI C'AATT_G Cuts at: 4218 4218 Size: 6619 MluI A'CGCG_T Cuts at: 2094 6495 2094 Size: 4401 2218 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2323 2712 2760 3093 5733 5917 1305 Size: 1018 389 48 333 2640 184 2007 Fragments arranged by size: 2640 2007 1018 389 333 184 48 MscI TGG'CCA Cuts at: 10 64 1489 1832 3611 10 Size: 54 1425 343 1779 3018 Fragments arranged by size: 3018 1779 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2408 3461 3509 4577 4936 5095 518 Size: 648 1242 1053 48 1068 359 159 2042 Fragments arranged by size: 2042 1242 1068 1053 648 359 159 48 MspA1I CmG'CkG Cuts at: 1606 3397 3691 4035 4712 5860 6105 1606 Size: 1791 294 344 677 1148 245 2120 Fragments arranged by size: 2120 1791 1148 677 344 294 245 NcoI C'CATG_G Cuts at: 513 1065 3459 3510 513 Size: 552 2394 51 3622 Fragments arranged by size: 3622 2394 552 51 NdeI CA'TA_TG Cuts at: 387 2236 387 Size: 1849 4770 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6619 NotI GC'GGCC_GC Cuts at: 4066 4066 Size: 6619 NspI r_CATG'y Cuts at: 1132 1229 2965 3220 3580 3977 5522 1132 Size: 97 1736 255 360 397 1545 2229 Fragments arranged by size: 2229 1736 1545 397 360 255 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6619 PciI A'CATG_T Cuts at: 5518 5518 Size: 6619 PflMI CCAn_nnn'nTGG Cuts at: 1108 3727 1108 Size: 2619 4000 PfoI T'CCnGG_A Cuts at: 3222 3222 Size: 6619 PmeI GTTT'AAAC Cuts at: 3923 3923 Size: 6619 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4688 4720 6201 6233 4688 Size: 32 1481 32 5074 Fragments arranged by size: 5074 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2594 2594 Size: 6619 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6619 PsiI TTA'TAA Cuts at: 4189 4189 Size: 6619 PstI C_TGCA'G Cuts at: 830 2121 2572 2891 3073 3198 3971 830 Size: 1291 451 319 182 125 773 3478 Fragments arranged by size: 3478 1291 773 451 319 182 125 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6619 PvuII CAG'CTG Cuts at: 3397 3691 3397 Size: 294 6325 SalI G'TCGA_C Cuts at: 1936 3961 1936 Size: 2025 4594 SbfI CC_TGCA'GG Cuts at: 3971 3971 Size: 6619 ScaI AGT'ACT Cuts at: 2791 4867 6579 2791 Size: 2076 1712 2831 Fragments arranged by size: 2831 2076 1712 SfcI C'TryA_G Cuts at: 826 1045 2117 2568 2887 3069 3170 3194 3967 Size: 219 1072 451 319 182 101 24 773 Cuts at: 3967 5102 5783 5974 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 773 681 451 319 219 191 182 101 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6619 SmaI CCC'GGG Cuts at: 1872 2741 3946 1872 Size: 869 1205 4545 Fragments arranged by size: 4545 1205 869 SmlI C'TyrA_G Cuts at: 820 1017 1943 2165 3001 4723 5624 5886 820 Size: 197 926 222 836 1722 901 262 1553 Fragments arranged by size: 1722 1553 926 901 836 262 222 197 SnaBI TAC'GTA Cuts at: 493 4410 493 Size: 3917 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6619 SphI G_CATG'C Cuts at: 1132 3580 3977 1132 Size: 2448 397 3774 Fragments arranged by size: 3774 2448 397 SspI AAT'ATT Cuts at: 5 52 4543 5 Size: 47 4491 2081 Fragments arranged by size: 4491 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6619 StyI C'CwwG_G Cuts at: 513 1065 2031 2484 2943 3459 3510 3538 3711 Size: 552 966 453 459 516 51 28 173 Cuts at: 3711 3888 4058 513 Size: 177 170 3074 Fragments arranged by size: 3074 966 552 516 459 453 177 173 170 51 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2162 2744 2945 4646 4663 4816 5001 Size: 1255 582 582 201 1701 17 153 185 Cuts at: 5001 5446 325 Size: 445 1498 Fragments arranged by size: 1701 1498 1255 582 582 445 201 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2789 4865 6577 Size: 274 80 33 51 1443 812 2076 1712 Cuts at: 6577 96 Size: 138 Fragments arranged by size: 2076 1712 1443 812 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6579 6611 6579 Size: 32 6587 Tth111I GACn'n_nGTC Cuts at: 1316 2857 1316 Size: 1541 5078 XbaI T'CTAG_A Cuts at: 3955 3955 Size: 6619 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2950 3841 3895 2950 Size: 891 54 5674 Fragments arranged by size: 5674 891 54 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6619 XmaI C'CCGG_G Cuts at: 1870 2739 3944 1870 Size: 869 1205 4545 Fragments arranged by size: 4545 1205 869 XmnI GAAnn'nnTTC Cuts at: 3341 4748 3341 Size: 1407 5212 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4427 276 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI