Restriction map for
pFN21AB5108
(Linear) (Six-base) MAPSORT of: KIBB5108.seq Check: 1267 from: 1 to: 2385
KIBB5108.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
Acc65I G'GTAC_C
Cuts at: 0 20 2385
Size: 20 2365
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 1684 1709 1745 1771 1838 2385
Size: 941 743 25 36 26 67 547
Fragments arranged by size:
941 743 547 67 36 26 25
AgeI A'CCGG_T
Cuts at: 0 259 2385
Size: 259 2126
AleI CACnn'nnGTG
Cuts at: 0 1871 2385
Size: 1871 514
AlwNI CAG_nnn'CTG
Cuts at: 0 1489 1510 2385
Size: 1489 21 875
Fragments arranged by size:
1489 875 21
ApaI G_GGCC'C
Cuts at: 0 141 2385
Size: 141 2244
ApoI r'AATT_y
Cuts at: 0 364 610 1198 1345 2376 2385
Size: 364 246 588 147 1031 9
Fragments arranged by size:
1031 588 364 246 147 9
AvaI C'yCGr_G
Cuts at: 0 113 2385
Size: 113 2272
BanI G'GyrC_C
Cuts at: 0 20 2385
Size: 20 2365
BanII G_rGCy'C
Cuts at: 0 141 1441 1911 2385
Size: 141 1300 470 474
Fragments arranged by size:
1300 474 470 141
BbsI GAAGACnn'nnnn_
Cuts at: 0 578 2385
Size: 578 1807
BfrBI ATG'CAT
Cuts at: 0 215 284 2385
Size: 215 69 2101
Fragments arranged by size:
2101 215 69
BglII A'GATC_T
Cuts at: 0 473 757 2385
Size: 473 284 1628
Fragments arranged by size:
1628 473 284
BlpI GC'TnA_GC
Cuts at: 0 28 1001 2385
Size: 28 973 1384
Fragments arranged by size:
1384 973 28
Bme1580I G_kGCm'C
Cuts at: 0 141 2385
Size: 141 2244
BmrI ACTGGGnnnn_n'
Cuts at: 0 1865 2385
Size: 1865 520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 1747 2385
Size: 210 1537 638
Fragments arranged by size:
1537 638 210
BsaI GGTCTCn'nnnn_
Cuts at: 0 110 2385
Size: 110 2275
BsaBI GATnn'nnATC
Cuts at: 0 1316 2385
Size: 1316 1069
BsaWI w'CCGG_w
Cuts at: 0 259 2385
Size: 259 2126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 86 116 1092 1122 2385
Size: 86 30 976 30 1263
Fragments arranged by size:
1263 976 86 30 30
BseYI C'CCAG_C
Cuts at: 0 1393 2385
Size: 1393 992
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1667 1820 2385
Size: 1667 153 565
Fragments arranged by size:
1667 565 153
BsiHKAI G_wGCw'C
Cuts at: 0 792 839 2385
Size: 792 47 1546
Fragments arranged by size:
1546 792 47
BsiWI C'GTAC_G
Cuts at: 0 60 2385
Size: 60 2325
BsmI GAATG_Cn'
Cuts at: 0 2362 2385
Size: 2362 23
Bsp1286I G_dGCh'C
Cuts at: 0 141 792 839 1441 1911 2385
Size: 141 651 47 602 470 474
Fragments arranged by size:
651 602 474 470 141 47
BspHI T'CATG_A
Cuts at: 0 2024 2385
Size: 2024 361
BsrBI CCG'CTC
Cuts at: 0 8 2385
Size: 8 2377
BsrDI GCAATG_nn'
Cuts at: 0 1899 2096 2385
Size: 1899 197 289
Fragments arranged by size:
1899 289 197
BsrFI r'CCGG_y
Cuts at: 0 23 259 2385
Size: 23 236 2126
Fragments arranged by size:
2126 236 23
BsrGI T'GTAC_A
Cuts at: 0 2277 2385
Size: 2277 108
BssSI C'ACGA_G
Cuts at: 0 49 2385
Size: 49 2336
BstAPI GCAn_nnn'nTGC
Cuts at: 0 631 2385
Size: 631 1754
BstYI r'GATC_y
Cuts at: 0 473 757 1404 1447 1473 2385
Size: 473 284 647 43 26 912
Fragments arranged by size:
912 647 473 284 43 26
BtgI C'CryG_G
Cuts at: 0 1529 2385
Size: 1529 856
BtsI GCAGTG_nn'
Cuts at: 0 398 464 2385
Size: 398 66 1921
Fragments arranged by size:
1921 398 66
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1215 1250 2385
Size: 1215 35 1135
Fragments arranged by size:
1215 1135 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1058 2385
Size: 1058 1327
EaeI y'GGCC_r
Cuts at: 0 186 1532 2004 2385
Size: 186 1346 472 381
Fragments arranged by size:
1346 472 381 186
EarI CTCTTCn'nnn_
Cuts at: 0 1446 1685 2385
Size: 1446 239 700
Fragments arranged by size:
1446 700 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 77 2385
Size: 77 2308
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 1684 1709 1745 1771 1838 2385
Size: 941 743 25 36 26 67 547
Fragments arranged by size:
941 743 547 67 36 26 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1481 2385
Size: 1481 904
EcoO109I rG'GnC_Cy
Cuts at: 0 66 138 2385
Size: 66 72 2247
Fragments arranged by size:
2247 72 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 742 774 1008 1040 1725 1757 2006 2038
Size: 742 32 234 32 685 32 249 32
Cuts at: 2038 2385
Size: 347
Fragments arranged by size:
742 685 347 249 234 32 32 32
32
HincII GTy'rAC
Cuts at: 0 500 1283 1703 2385
Size: 500 783 420 682
Fragments arranged by size:
783 682 500 420
HpaI GTT'AAC
Cuts at: 0 1283 1703 2385
Size: 1283 420 682
Fragments arranged by size:
1283 682 420
KpnI G_GTAC'C
Cuts at: 0 24 2385
Size: 24 2361
MfeI C'AATT_G
Cuts at: 0 1217 1992 2385
Size: 1217 775 393
Fragments arranged by size:
1217 775 393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 825 2385
Size: 825 1560
MscI TGG'CCA
Cuts at: 0 188 2006 2385
Size: 188 1818 379
Fragments arranged by size:
1818 379 188
MslI CAynn'nnrTG
Cuts at: 0 1028 1871 2385
Size: 1028 843 514
Fragments arranged by size:
1028 843 514
MspA1I CmG'CkG
Cuts at: 0 454 1576 1968 2385
Size: 454 1122 392 417
Fragments arranged by size:
1122 454 417 392
NcoI C'CATG_G
Cuts at: 0 1529 2385
Size: 1529 856
NsiI A_TGCA'T
Cuts at: 0 217 286 2385
Size: 217 69 2099
Fragments arranged by size:
2099 217 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 54 2385
Size: 54 2331
PspOMI G'GGCC_C
Cuts at: 0 137 2385
Size: 137 2248
PstI C_TGCA'G
Cuts at: 0 459 2385
Size: 459 1926
PvuII CAG'CTG
Cuts at: 0 454 1576 1968 2385
Size: 454 1122 392 417
Fragments arranged by size:
1122 454 417 392
SapI GCTCTTCn'nnn_
Cuts at: 0 1446 2385
Size: 1446 939
SexAI A'CCwGG_T
Cuts at: 0 16 1035 1503 2385
Size: 16 1019 468 882
Fragments arranged by size:
1019 882 468 16
SfcI C'TryA_G
Cuts at: 0 455 2361 2385
Size: 455 1906 24
Fragments arranged by size:
1906 455 24
SgrAI Cr'CCGG_yG
Cuts at: 0 259 2385
Size: 259 2126
SmlI C'TyrA_G
Cuts at: 0 225 1726 2385
Size: 225 1501 659
Fragments arranged by size:
1501 659 225
SspI AAT'ATT
Cuts at: 0 1596 1652 2385
Size: 1596 56 733
Fragments arranged by size:
1596 733 56
StyI C'CwwG_G
Cuts at: 0 1276 1529 2385
Size: 1276 253 856
Fragments arranged by size:
1276 856 253
TatI w'GTAC_w
Cuts at: 0 2205 2277 2385
Size: 2205 72 108
Fragments arranged by size:
2205 108 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 86 118 1092 1124 1458 1490 2385
Size: 86 32 974 32 334 32 895
Fragments arranged by size:
974 895 334 86 32 32 32
Enzymes that do cut:
Acc65I AcuI AgeI AleI AlwNI ApaI ApoI AvaI
BanI BanII BbsI BfrBI BglII BlpI Bme1580I BmrI
BpuEI BsaI BsaBI BsaWI BsaXI BseYI BsgI BsiHKAI
BsiWI BsmI Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstYI BtgI BtsI CspCI DrdI EaeI
EarI EciI Eco57MI EcoNI EcoO109I Hin4I HincII HpaI
KpnI MfeI MmeI MscI MslI MspA1I NcoI NsiI
PflMI PspOMI PstI PvuII SapI SexAI SfcI SgrAI
SmlI SspI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BglI BmgBI
BmtI BplI BpmI Bpu10I BsaAI BsaHI BseRI BsiEI
BsmBI BspEI BspMI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI DraI DraIII EagI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII KasI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI