(Circular) (Six-base) MAPSORT of: pFN21AB5101.seq Check: 5401 from: 1 to: 7852 pFN21AB5101 7852 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2537 2537 Size: 7852 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3009 5662 278 Size: 53 83 186 936 1473 2653 2468 Fragments arranged by size: 2653 2468 1473 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2292 3099 3105 3462 5047 5195 1937 Size: 355 807 6 357 1585 148 4594 Fragments arranged by size: 4594 1585 807 357 355 148 6 Acc65I G'GTAC_C Cuts at: 5173 5173 Size: 7852 AclI AA'CG_TT Cuts at: 1563 5979 6352 1563 Size: 4416 373 3063 Fragments arranged by size: 4416 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2614 3626 4916 5915 7299 7567 1632 Size: 982 1012 1290 999 1384 268 1917 Fragments arranged by size: 1917 1384 1290 1012 999 982 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7655 AflIII A'CryG_T Cuts at: 1203 3607 6751 7728 1203 Size: 2404 3144 977 1327 Fragments arranged by size: 3144 2404 1327 977 AgeI A'CCGG_T Cuts at: 6683 6683 Size: 7852 AhdI GACnn_n'nnGTC Cuts at: 4478 6581 4478 Size: 2103 5749 AleI CACnn'nnGTG Cuts at: 1166 4958 1166 Size: 3792 4060 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2301 1184 Size: 1117 6735 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4379 4411 4379 Size: 32 7820 AlwNI CAG_nnn'CTG Cuts at: 2704 3331 3340 3724 4106 4561 4729 5595 7167 Size: 627 9 384 382 455 168 866 1572 Cuts at: 7167 2704 Size: 3389 Fragments arranged by size: 3389 1572 866 627 455 384 382 168 9 ApaI G_GGCC'C Cuts at: 2060 2169 3299 4779 2060 Size: 109 1130 1480 5133 Fragments arranged by size: 5133 1480 1130 109 ApaLI G'TGCA_C Cuts at: 2326 5909 7065 2326 Size: 3583 1156 3113 Fragments arranged by size: 3583 3113 1156 ApoI r'AATT_y Cuts at: 1493 2339 2559 2843 3791 5161 5387 5539 7648 Size: 846 220 284 948 1370 226 152 2109 Cuts at: 7648 1493 Size: 1697 Fragments arranged by size: 2109 1697 1370 948 846 284 226 220 152 AseI AT'TA_AT Cuts at: 160 1032 6406 160 Size: 872 5374 1606 Fragments arranged by size: 5374 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7852 AvaI C'yCGr_G Cuts at: 1870 1943 2161 2170 2744 3744 4440 4655 4714 Size: 73 218 9 574 1000 696 215 59 Cuts at: 4714 4772 5177 1870 Size: 58 405 4545 Fragments arranged by size: 4545 1000 696 574 405 218 215 73 59 58 9 AvrII C'CTAG_G Cuts at: 2818 5041 2818 Size: 2223 5629 BamHI G'GATC_C Cuts at: 4651 5182 5549 4651 Size: 531 367 6954 Fragments arranged by size: 6954 531 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2064 2309 2525 3043 4064 Size: 325 217 627 277 245 216 518 1021 Cuts at: 4064 5102 5173 6628 618 Size: 1038 71 1455 1842 Fragments arranged by size: 1842 1455 1038 1021 627 518 325 277 245 217 216 71 BanII G_rGCy'C Cuts at: 1391 2060 2169 2177 2449 3135 3247 3299 3314 Size: 669 109 8 272 686 112 52 15 Cuts at: 3314 3745 4500 4773 4779 5171 1391 Size: 431 755 273 6 392 4072 Fragments arranged by size: 4072 755 686 669 431 392 273 272 112 109 52 15 8 6 BbeI G_GCGC'C Cuts at: 2068 4068 2068 Size: 2000 5852 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2871 3951 928 Size: 971 972 1080 4829 Fragments arranged by size: 4829 1080 972 971 BbvCI CC'TCA_GC Cuts at: 4307 4307 Size: 7852 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2142 2176 3735 3769 6043 6077 1903 Size: 34 205 34 1559 34 2274 34 3678 Fragments arranged by size: 3678 2274 1559 205 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2816 5744 6954 2816 Size: 2928 1210 3714 Fragments arranged by size: 3714 2928 1210 BclI T'GATC_A Cuts at: 1548 2754 1548 Size: 1206 6646 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2625 3180 3242 Size: 107 122 71 631 605 953 555 62 Cuts at: 3242 4073 6463 136 Size: 831 2390 1525 Fragments arranged by size: 2390 1525 953 831 631 605 555 122 107 71 62 BglII A'GATC_T Cuts at: 3768 3924 7847 3768 Size: 156 3923 3773 Fragments arranged by size: 3923 3773 156 BlpI GC'TnA_GC Cuts at: 4077 5121 5269 4077 Size: 1044 148 6660 Fragments arranged by size: 6660 1044 148 Bme1580I G_kGCm'C Cuts at: 1790 2060 2169 2330 2528 2886 3046 3299 3685 Size: 270 109 161 198 358 160 253 386 Cuts at: 3685 4779 5913 7069 1790 Size: 1094 1134 1156 2573 Fragments arranged by size: 2573 1156 1134 1094 386 358 270 253 198 161 160 109 BmgBI CAC'GTC Cuts at: 1318 4339 1318 Size: 3021 4831 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3848 4415 5583 Size: 416 53 83 399 24 2431 567 1168 Cuts at: 5583 6541 442 Size: 958 1753 Fragments arranged by size: 2431 1753 1168 958 567 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7852 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3821 3821 Size: 7852 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2387 2585 3682 4083 4129 4664 5118 6512 Size: 1148 198 1097 401 46 535 454 1394 Cuts at: 6512 1239 Size: 2579 Fragments arranged by size: 2579 1394 1148 1097 535 454 401 198 46 Bpu10I CC'TnA_GC Cuts at: 3578 4151 4307 4368 3578 Size: 573 156 61 7062 Fragments arranged by size: 7062 573 156 61 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5111 5977 6842 7140 5111 Size: 866 865 298 5823 Fragments arranged by size: 5823 866 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3254 3390 4437 6515 882 Size: 617 1755 136 1047 2078 2219 Fragments arranged by size: 2219 2078 1755 1047 617 136 BsaAI yAC'GTr Cuts at: 493 1204 5643 493 Size: 711 4439 2702 Fragments arranged by size: 4439 2702 711 BsaBI GATnn'nnATC Cuts at: 3767 3818 5009 7846 3767 Size: 51 1191 2837 3773 Fragments arranged by size: 3773 2837 1191 51 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 2065 3006 4065 Size: 53 83 186 936 485 47 941 1059 Cuts at: 4065 4992 5659 6041 6676 275 Size: 927 667 382 635 1451 Fragments arranged by size: 1451 1059 941 936 927 667 635 485 382 186 83 53 47 BsaWI w'CCGG_w Cuts at: 6285 6683 6957 7104 7515 6285 Size: 398 274 147 411 6622 Fragments arranged by size: 6622 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2399 2429 4081 4111 4379 Size: 30 165 30 996 30 1652 30 268 Cuts at: 4379 4409 4733 4763 5076 5106 1178 Size: 30 324 30 313 30 3924 Fragments arranged by size: 3924 1652 996 324 313 268 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2411 2446 3402 3545 4475 1186 Size: 1225 35 956 143 930 4563 Fragments arranged by size: 4563 1225 956 930 143 35 BseYI C'CCAG_C Cuts at: 2052 2149 2595 3118 3264 3307 4514 7055 7551 Size: 97 446 523 146 43 1207 2541 496 Cuts at: 7551 2052 Size: 2353 Fragments arranged by size: 2541 2353 1207 523 496 446 146 97 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 4594 4652 4766 1163 Size: 573 327 2531 58 114 4249 Fragments arranged by size: 4249 2531 573 327 114 58 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2210 5302 6063 7091 1540 Size: 275 186 209 3092 761 1028 2301 Fragments arranged by size: 3092 2301 1028 761 275 209 186 BsiHKAI G_wGCw'C Cuts at: 2245 2330 4500 4773 4854 5913 5998 7069 2245 Size: 85 2170 273 81 1059 85 1071 3028 Fragments arranged by size: 3028 2170 1071 1059 273 85 85 81 BsmI GAATG_Cn' Cuts at: 4024 5363 5456 4024 Size: 1339 93 6420 Fragments arranged by size: 6420 1339 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2169 2177 2245 2330 2449 2528 Size: 399 270 109 8 68 85 119 79 Cuts at: 2528 2886 3046 3135 3247 3299 3314 3685 3745 Size: 358 160 89 112 52 15 371 60 Cuts at: 3745 4500 4773 4779 4854 5171 5913 5998 7069 Size: 755 273 6 75 317 742 85 1071 Cuts at: 7069 1391 Size: 2174 Fragments arranged by size: 2174 1071 755 742 399 371 358 317 273 270 160 119 112 109 89 85 85 79 75 68 60 52 15 8 6 BspEI T'CCGG_A Cuts at: 7515 7515 Size: 7852 BspHI T'CATG_A Cuts at: 5741 7471 5741 Size: 1730 6122 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2537 3117 3975 4483 5207 844 Size: 1693 580 858 508 724 3489 Fragments arranged by size: 3489 1693 858 724 580 508 BsrBI CCG'CTC Cuts at: 1394 1823 2583 3535 5739 1394 Size: 429 760 952 2204 3507 Fragments arranged by size: 3507 2204 952 760 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3415 4970 6347 6521 65 Size: 1183 2167 1555 1377 174 1396 Fragments arranged by size: 2167 1555 1396 1377 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2205 3122 6496 6683 1700 Size: 110 395 917 3374 187 2869 Fragments arranged by size: 3374 2869 917 395 187 110 BsrGI T'GTAC_A Cuts at: 96 2481 4884 96 Size: 2385 2403 3064 Fragments arranged by size: 3064 2403 2385 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7852 BssSI C'ACGA_G Cuts at: 5912 6924 5912 Size: 1012 6840 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7852 BstBI TT'CG_AA Cuts at: 6656 6656 Size: 7852 BstEII G'GTnAC_C Cuts at: 1184 4087 4378 1184 Size: 2903 291 4658 Fragments arranged by size: 4658 2903 291 BstYI r'GATC_y Cuts at: 1972 2700 3768 3924 4302 4651 4725 5182 5549 Size: 728 1068 156 378 349 74 457 367 Cuts at: 5549 5934 5951 7403 7489 7501 7847 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1068 728 457 385 378 367 349 346 156 86 74 17 12 BstZ17I GTA'TAC Cuts at: 3463 3463 Size: 7852 Bsu36I CC'TnA_GG Cuts at: 3081 3776 3872 4404 3081 Size: 695 96 532 6529 Fragments arranged by size: 6529 695 532 96 BtgI C'CryG_G Cuts at: 513 1065 2004 2449 2520 3865 4359 5109 513 Size: 552 939 445 71 1345 494 750 3256 Fragments arranged by size: 3256 1345 939 750 552 494 445 71 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3805 487 Size: 683 56 2579 4534 Fragments arranged by size: 4534 2579 683 56 BtsI GCAGTG_nn' Cuts at: 2429 2997 4416 4877 5368 6162 6182 2429 Size: 568 1419 461 491 794 20 4099 Fragments arranged by size: 4099 1419 794 568 491 461 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7852 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7817 DraI TTT'AAA Cuts at: 5156 5156 Size: 7852 DraIII CAC_nnn'GTG Cuts at: 2041 2325 2041 Size: 284 7568 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2023 6859 809 Size: 507 707 4836 1802 Fragments arranged by size: 4836 1802 707 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2207 2653 2994 Size: 54 1425 50 275 18 377 446 341 Cuts at: 2994 3157 3172 3234 4950 5299 6188 7817 8 Size: 163 15 62 1716 349 889 1629 43 Fragments arranged by size: 1716 1629 1425 889 446 377 349 341 275 163 62 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2207 5299 1537 Size: 275 395 3092 4090 Fragments arranged by size: 4090 3092 395 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5782 1353 Size: 4429 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6439 6813 6959 387 Size: 1064 138 4850 374 146 1280 Fragments arranged by size: 4850 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4498 4771 4498 Size: 273 7579 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2387 2585 2614 3626 3682 4083 4129 Size: 393 755 198 29 1012 56 401 46 Cuts at: 4129 4664 4916 5118 5915 6512 7299 7567 1239 Size: 535 252 202 797 597 787 268 1524 Fragments arranged by size: 1524 1012 797 787 755 597 535 401 393 268 252 202 198 56 46 29 EcoNI CCTnn'n_nnAGG Cuts at: 2765 3212 2765 Size: 447 7405 EcoO109I rG'GnC_Cy Cuts at: 2056 2057 2146 2165 2166 3205 3295 3435 4465 Size: 1 89 19 1 1039 90 140 1030 Cuts at: 4465 4472 4511 4639 4718 4776 2056 Size: 7 39 128 79 58 5132 Fragments arranged by size: 5132 1039 1030 140 128 90 89 79 58 39 19 7 1 1 EcoRI G'AATT_C Cuts at: 2559 2843 5161 5539 2559 Size: 284 2318 378 4872 Fragments arranged by size: 4872 2318 378 284 EcoRV GAT'ATC Cuts at: 3416 3416 Size: 7852 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4855 4855 Size: 7852 FspI TGC'GCA Cuts at: 6358 6358 Size: 7852 HaeII r_GCGC'y Cuts at: 1678 2068 4068 6999 1678 Size: 390 2000 2931 2531 Fragments arranged by size: 2931 2531 2000 390 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2399 2431 2924 2956 3791 Size: 32 135 32 827 32 493 32 835 Cuts at: 3791 3823 4464 4496 4710 4742 4786 4818 6493 Size: 32 641 32 214 32 44 32 1675 Cuts at: 6493 6525 6567 6599 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1675 835 827 641 493 214 135 44 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5196 5442 669 Size: 988 281 3258 246 3079 Fragments arranged by size: 3258 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 5069 748 Size: 4321 3531 HpaI GTT'AAC Cuts at: 5442 5442 Size: 7852 KasI G'GCGC_C Cuts at: 2064 4064 2064 Size: 2000 5852 KpnI G_GTAC'C Cuts at: 5177 5177 Size: 7852 MfeI C'AATT_G Cuts at: 5451 5451 Size: 7852 MluI A'CGCG_T Cuts at: 7728 7728 Size: 7852 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4629 6966 7150 1305 Size: 3324 2337 184 2007 Fragments arranged by size: 3324 2337 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3159 3236 4952 10 Size: 54 1425 343 1327 77 1716 2910 Fragments arranged by size: 2910 1716 1425 1327 343 77 54 MslI CAynn'nnrTG Cuts at: 518 1166 3956 4491 4958 5108 5810 6169 6328 Size: 648 2790 535 467 150 702 359 159 Cuts at: 6328 518 Size: 2042 Fragments arranged by size: 2790 2042 702 648 535 467 359 159 150 MspA1I CmG'CkG Cuts at: 1606 2047 2077 2086 2487 2595 2776 3646 3973 Size: 441 30 9 401 108 181 870 327 Cuts at: 3973 4548 4846 4927 4958 5268 5945 7093 7338 Size: 575 298 81 31 310 677 1148 245 Cuts at: 7338 1606 Size: 2120 Fragments arranged by size: 2120 1148 870 677 575 441 401 327 310 298 245 181 108 81 31 30 9 NaeI GCC'GGC Cuts at: 3124 3124 Size: 7852 NarI GG'CG_CC Cuts at: 2065 4065 2065 Size: 2000 5852 NcoI C'CATG_G Cuts at: 513 1065 2004 2449 5109 513 Size: 552 939 445 2660 3256 Fragments arranged by size: 3256 2660 939 552 445 NdeI CA'TA_TG Cuts at: 387 5098 387 Size: 4711 3141 NgoMIV G'CCGG_C Cuts at: 3122 3122 Size: 7852 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7852 NotI GC'GGCC_GC Cuts at: 5299 5299 Size: 7852 NspI r_CATG'y Cuts at: 1132 1229 2472 5210 6755 1132 Size: 97 1243 2738 1545 2229 Fragments arranged by size: 2738 2229 1545 1243 97 PasI CC'CwG_GG Cuts at: 1348 2115 3199 4133 1348 Size: 767 1084 934 5067 Fragments arranged by size: 5067 1084 934 767 PciI A'CATG_T Cuts at: 6751 6751 Size: 7852 PflMI CCAn_nnn'nTGG Cuts at: 1108 4051 4233 4561 1108 Size: 2943 182 328 4399 Fragments arranged by size: 4399 2943 328 182 PfoI T'CCnGG_A Cuts at: 2364 4266 2364 Size: 1902 5950 PmeI GTTT'AAAC Cuts at: 5156 5156 Size: 7852 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4379 4411 5921 5953 7434 7466 4379 Size: 32 1510 32 1481 32 4765 Fragments arranged by size: 4765 1510 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3205 3435 4465 4472 4718 3205 Size: 230 1030 7 246 6339 Fragments arranged by size: 6339 1030 246 230 7 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7852 PsiI TTA'TAA Cuts at: 5422 5422 Size: 7852 PspOMI G'GGCC_C Cuts at: 2056 2165 3295 4775 2056 Size: 109 1130 1480 5133 Fragments arranged by size: 5133 1480 1130 109 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2885 2917 3986 4018 2885 Size: 32 1069 32 6719 Fragments arranged by size: 6719 1069 32 32 PstI C_TGCA'G Cuts at: 830 2280 2781 3114 3336 3545 3972 4809 4872 Size: 1450 501 333 222 209 427 837 63 Cuts at: 4872 5204 830 Size: 332 3478 Fragments arranged by size: 3478 1450 837 501 427 333 332 222 209 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7852 PvuII CAG'CTG Cuts at: 2047 2487 2595 2776 3646 3973 4548 4927 4958 Size: 440 108 181 870 327 575 379 31 Cuts at: 4958 2047 Size: 4941 Fragments arranged by size: 4941 870 575 440 379 327 181 108 31 SacI G_AGCT'C Cuts at: 4500 4773 4500 Size: 273 7579 SalI G'TCGA_C Cuts at: 1936 5194 1936 Size: 3258 4594 SbfI CC_TGCA'GG Cuts at: 5204 5204 Size: 7852 ScaI AGT'ACT Cuts at: 2157 4112 6100 7812 2157 Size: 1955 1988 1712 2197 Fragments arranged by size: 2197 1988 1955 1712 SexAI A'CCwGG_T Cuts at: 2760 2760 Size: 7852 SfcI C'TryA_G Cuts at: 826 1045 2276 2777 3110 3332 3541 3968 4805 Size: 219 1231 501 333 222 209 427 837 Cuts at: 4805 4868 5200 6335 7016 7207 826 Size: 63 332 1135 681 191 1471 Fragments arranged by size: 1471 1231 1135 837 681 501 427 333 332 222 219 209 191 63 SfoI GGC'GCC Cuts at: 2066 4066 2066 Size: 2000 5852 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7852 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7852 SmaI CCC'GGG Cuts at: 1872 2172 5179 1872 Size: 300 3007 4545 Fragments arranged by size: 4545 3007 300 SmlI C'TyrA_G Cuts at: 820 1017 1943 3744 5126 5956 6857 7119 820 Size: 197 926 1801 1382 830 901 262 1553 Fragments arranged by size: 1801 1553 1382 926 901 830 262 197 SnaBI TAC'GTA Cuts at: 493 5643 493 Size: 5150 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7852 SphI G_CATG'C Cuts at: 1132 2472 5210 1132 Size: 1340 2738 3774 Fragments arranged by size: 3774 2738 1340 SspI AAT'ATT Cuts at: 5 52 5776 5 Size: 47 5724 2081 Fragments arranged by size: 5724 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7852 StyI C'CwwG_G Cuts at: 513 1065 2004 2247 2303 2449 2620 2818 3837 Size: 552 939 243 56 146 171 198 1019 Cuts at: 3837 4507 4971 5041 5109 5291 513 Size: 670 464 70 68 182 3074 Fragments arranged by size: 3074 1019 939 670 552 464 243 198 182 171 146 70 68 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2128 2260 3132 3998 4043 4146 5879 Size: 1255 548 132 872 866 45 103 1733 Cuts at: 5879 5896 6049 6234 6679 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1733 1498 1255 872 866 548 445 185 153 132 103 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2155 2409 2481 Size: 274 80 33 51 1443 178 254 72 Cuts at: 2481 4110 4884 6098 7810 96 Size: 1629 774 1214 1712 138 Fragments arranged by size: 1712 1629 1443 1214 774 274 254 178 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2178 2210 2814 2846 4081 4113 4733 4765 7812 Size: 32 604 32 1235 32 620 32 3047 Cuts at: 7812 7844 2178 Size: 32 2186 Fragments arranged by size: 3047 2186 1235 620 604 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2222 1316 Size: 906 6946 XbaI T'CTAG_A Cuts at: 5188 5188 Size: 7852 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2484 3167 4428 2484 Size: 683 1261 5908 Fragments arranged by size: 5908 1261 683 XhoI C'TCGA_G Cuts at: 1943 3744 1943 Size: 1801 6051 XmaI C'CCGG_G Cuts at: 1870 2170 5177 1870 Size: 300 3007 4545 Fragments arranged by size: 4545 3007 300 XmnI GAAnn'nnTTC Cuts at: 5981 5981 Size: 7852 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3007 5660 276 Size: 53 83 186 936 1473 2653 2468 Fragments arranged by size: 2653 2468 1473 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BfrBI BsiWI BsmBI BstXI FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SacII SanDI SapI SfiI SrfI SwaI