Restriction map for pFN21AB5101
 (Linear) (Six-base) MAPSORT of: KIBB5101.seq  Check: 8437  from: 1  to: 3147



KIBB5101.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     532    3147 
   Size:        532    2615
 
AatII G_ACGT'C

Cuts at:      0    1004    3147 
   Size:       1004    2143
 
AccI GT'mk_AC

Cuts at:      0     287    1094    1100    1457    3042    3147 
   Size:        287     807       6     357    1585     105

  Fragments arranged by size: 

                1585     807     357     287     105       6
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     609    1621    2911    3147 
   Size:        609    1012    1290     236

  Fragments arranged by size: 

                1290    1012     609     236
 
AflIII A'CryG_T

Cuts at:      0    1602    3147 
   Size:       1602    1545
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2473    3147 
   Size:       2473     674
 
AleI CACnn'nnGTG

Cuts at:      0    2953    3147 
   Size:       2953     194
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     296    3147 
   Size:        296    2851
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2374    2406    3147 
   Size:       2374      32     741

  Fragments arranged by size: 

                2374     741      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     699    1326    1335    1719    2101    2556    2724    3147 
   Size:        699     627       9     384     382     455     168     423

  Fragments arranged by size: 

                 699     627     455     423     384     382     168       9
            
 
ApaI G_GGCC'C

Cuts at:      0      55     164    1294    2774    3147 
   Size:         55     109    1130    1480     373

  Fragments arranged by size: 

                1480    1130     373     109      55
 
ApaLI G'TGCA_C

Cuts at:      0     321    3147 
   Size:        321    2826
 
ApoI r'AATT_y

Cuts at:      0     334     554     838    1786    3147 
   Size:        334     220     284     948    1361

  Fragments arranged by size: 

                1361     948     334     284     220
 
AvaI C'yCGr_G

Cuts at:      0     156     165     739    1739    2435    2650    2709    2767 
   Size:        156       9     574    1000     696     215      59      58

Cuts at:   2767    3147 
   Size:        380

  Fragments arranged by size: 

                1000     696     574     380     215     156      59      58
                   9
 
AvrII C'CTAG_G

Cuts at:      0     813    3036    3147 
   Size:        813    2223     111

  Fragments arranged by size: 

                2223     813     111
 
BamHI G'GATC_C

Cuts at:      0    2646    3147 
   Size:       2646     501
 
BanI G'GyrC_C

Cuts at:      0      59     304     520    1038    2059    3097    3147 
   Size:         59     245     216     518    1021    1038      50

  Fragments arranged by size: 

                1038    1021     518     245     216      59      50
 
BanII G_rGCy'C

Cuts at:      0      55     164     172     444    1130    1242    1294    1309 
   Size:         55     109       8     272     686     112      52      15

Cuts at:   1309    1740    2495    2768    2774    3147 
   Size:        431     755     273       6     373

  Fragments arranged by size: 

                 755     686     431     373     273     272     112     109
                  55      52      15       8       6
 
BbeI G_GCGC'C

Cuts at:      0      63    2063    3147 
   Size:         63    2000    1084

  Fragments arranged by size: 

                2000    1084      63
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     866    1946    3147 
   Size:        866    1080    1201

  Fragments arranged by size: 

                1201    1080     866
 
BbvCI CC'TCA_GC

Cuts at:      0    2302    3147 
   Size:       2302     845
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     137     171    1730    1764    3147 
   Size:        137      34    1559      34    1383

  Fragments arranged by size: 

                1559    1383     137      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     811    3147 
   Size:        811    2336
 
BclI T'GATC_A

Cuts at:      0     749    3147 
   Size:        749    2398
 
BglI GCCn_nnn'nGGC

Cuts at:      0     620    1175    1237    2068    3147 
   Size:        620     555      62     831    1079

  Fragments arranged by size: 

                1079     831     620     555      62
 
BglII A'GATC_T

Cuts at:      0    1763    1919    3147 
   Size:       1763     156    1228

  Fragments arranged by size: 

                1763    1228     156
 
BlpI GC'TnA_GC

Cuts at:      0    2072    3116    3147 
   Size:       2072    1044      31

  Fragments arranged by size: 

                2072    1044      31
 
Bme1580I G_kGCm'C

Cuts at:      0      55     164     325     523     881    1041    1294    1680 
   Size:         55     109     161     198     358     160     253     386

Cuts at:   1680    2774    3147 
   Size:       1094     373

  Fragments arranged by size: 

                1094     386     373     358     253     198     161     160
                 109      55
 
BmgBI CAC'GTC

Cuts at:      0    2334    3147 
   Size:       2334     813
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1843    2410    3147 
   Size:       1843     567     737

  Fragments arranged by size: 

                1843     737     567
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1816    3147 
   Size:       1816    1331
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     580    1677    2078    2124    2659    3113    3147 
   Size:        382     198    1097     401      46     535     454      34

  Fragments arranged by size: 

                1097     535     454     401     382     198      46      34
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1573    2146    2302    2363    3147 
   Size:       1573     573     156      61     784

  Fragments arranged by size: 

                1573     784     573     156      61
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3106    3147 
   Size:       3106      41
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1249    1385    2432    3147 
   Size:       1249     136    1047     715

  Fragments arranged by size: 

                1249    1047     715     136
 
BsaBI GATnn'nnATC

Cuts at:      0    1762    1813    3004    3147 
   Size:       1762      51    1191     143

  Fragments arranged by size: 

                1762    1191     143      51
 
BsaHI Gr'CG_yC

Cuts at:      0      13      60    1001    2060    2987    3147 
   Size:         13      47     941    1059     927     160

  Fragments arranged by size: 

                1059     941     927     160      47      13
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     394     424    2076    2106    2374    2404    2728    2758 
   Size:        394      30    1652      30     268      30     324      30

Cuts at:   2758    3071    3101    3147 
   Size:        313      30      46

  Fragments arranged by size: 

                1652     394     324     313     268      46      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     406     441    1397    1540    2470    3147 
   Size:        406      35     956     143     930     677

  Fragments arranged by size: 

                 956     930     677     406     143      35
 
BseYI C'CCAG_C

Cuts at:      0      47     144     590    1113    1259    1302    2509    3147 
   Size:         47      97     446     523     146      43    1207     638

  Fragments arranged by size: 

                1207     638     523     446     146      97      47      43
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58    2589    2647    2761    3147 
   Size:         58    2531      58     114     386

  Fragments arranged by size: 

                2531     386     114      58      58
 
BsiEI CG_ry'CG

Cuts at:      0     205    3147 
   Size:        205    2942
 
BsiHKAI G_wGCw'C

Cuts at:      0     240     325    2495    2768    2849    3147 
   Size:        240      85    2170     273      81     298

  Fragments arranged by size: 

                2170     298     273     240      85      81
 
BsmI GAATG_Cn'

Cuts at:      0    2019    3147 
   Size:       2019    1128
 
Bsp1286I G_dGCh'C

Cuts at:      0      55     164     172     240     325     444     523     881 
   Size:         55     109       8      68      85     119      79     358

Cuts at:    881    1041    1130    1242    1294    1309    1680    1740    2495 
   Size:        160      89     112      52      15     371      60     755

Cuts at:   2495    2768    2774    2849    3147 
   Size:        273       6      75     298

  Fragments arranged by size: 

                 755     371     358     298     273     160     119     112
                 109      89      85      79      75      68      60      55
                  52      15       8       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     532    1112    1970    2478    3147 
   Size:        532     580     858     508     669

  Fragments arranged by size: 

                 858     669     580     532     508
 
BsrBI CCG'CTC

Cuts at:      0     578    1530    3147 
   Size:        578     952    1617

  Fragments arranged by size: 

                1617     952     578
 
BsrDI GCAATG_nn'

Cuts at:      0    1410    2965    3147 
   Size:       1410    1555     182

  Fragments arranged by size: 

                1555    1410     182
 
BsrFI r'CCGG_y

Cuts at:      0     200    1117    3147 
   Size:        200     917    2030

  Fragments arranged by size: 

                2030     917     200
 
BsrGI T'GTAC_A

Cuts at:      0     476    2879    3147 
   Size:        476    2403     268

  Fragments arranged by size: 

                2403     476     268
 
BstEII G'GTnAC_C

Cuts at:      0    2082    2373    3147 
   Size:       2082     291     774

  Fragments arranged by size: 

                2082     774     291
 
BstYI r'GATC_y

Cuts at:      0     695    1763    1919    2297    2646    2720    3147 
   Size:        695    1068     156     378     349      74     427

  Fragments arranged by size: 

                1068     695     427     378     349     156      74
 
BstZ17I GTA'TAC

Cuts at:      0    1458    3147 
   Size:       1458    1689
 
Bsu36I CC'TnA_GG

Cuts at:      0    1076    1771    1867    2399    3147 
   Size:       1076     695      96     532     748

  Fragments arranged by size: 

                1076     748     695     532      96
 
BtgI C'CryG_G

Cuts at:      0     444     515    1860    2354    3104    3147 
   Size:        444      71    1345     494     750      43

  Fragments arranged by size: 

                1345     750     494     444      71      43
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1800    3147 
   Size:       1800    1347
 
BtsI GCAGTG_nn'

Cuts at:      0     424     992    2411    2872    3147 
   Size:        424     568    1419     461     275

  Fragments arranged by size: 

                1419     568     461     424     275
 
DraIII CAC_nnn'GTG

Cuts at:      0      36     320    3147 
   Size:         36     284    2827

  Fragments arranged by size: 

                2827     284      36
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      18    3147 
   Size:         18    3129
 
EaeI y'GGCC_r

Cuts at:      0     202     648     989    1152    1167    1229    2945    3147 
   Size:        202     446     341     163      15      62    1716     202

  Fragments arranged by size: 

                1716     446     341     202     202     163      62      15
            
 
EagI C'GGCC_G

Cuts at:      0     202    3147 
   Size:        202    2945
 
EcoICRI GAG'CTC

Cuts at:      0    2493    2766    3147 
   Size:       2493     273     381

  Fragments arranged by size: 

                2493     381     273
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     580     609    1621    1677    2078    2124    2659 
   Size:        382     198      29    1012      56     401      46     535

Cuts at:   2659    2911    3113    3147 
   Size:        252     202      34

  Fragments arranged by size: 

                1012     535     401     382     252     202     198      56
                  46      34      29
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     760    1207    3147 
   Size:        760     447    1940

  Fragments arranged by size: 

                1940     760     447
 
EcoO109I rG'GnC_Cy

Cuts at:      0      51      52     141     160     161    1200    1290    1430 
   Size:         51       1      89      19       1    1039      90     140

Cuts at:   1430    2460    2467    2506    2634    2713    2771    3147 
   Size:       1030       7      39     128      79      58     376

  Fragments arranged by size: 

                1039    1030     376     140     128      90      89      79
                  58      51      39      19       7       1       1
 
EcoRI G'AATT_C

Cuts at:      0     554     838    3147 
   Size:        554     284    2309

  Fragments arranged by size: 

                2309     554     284
 
EcoRV GAT'ATC

Cuts at:      0    1411    3147 
   Size:       1411    1736
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2850    3147 
   Size:       2850     297
 
HaeII r_GCGC'y

Cuts at:      0      63    2063    3147 
   Size:         63    2000    1084

  Fragments arranged by size: 

                2000    1084      63
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     394     426     919     951    1786    1818    2459    2491 
   Size:        394      32     493      32     835      32     641      32

Cuts at:   2491    2705    2737    2781    2813    3147 
   Size:        214      32      44      32     334

  Fragments arranged by size: 

                 835     641     493     394     334     214      44      32
                  32      32      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    3064    3147 
   Size:       3064      83
 
KasI G'GCGC_C

Cuts at:      0      59    2059    3147 
   Size:         59    2000    1088

  Fragments arranged by size: 

                2000    1088      59
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2624    3147 
   Size:       2624     523
 
MscI TGG'CCA

Cuts at:      0    1154    1231    2947    3147 
   Size:       1154      77    1716     200

  Fragments arranged by size: 

                1716    1154     200      77
 
MslI CAynn'nnrTG

Cuts at:      0    1951    2486    2953    3103    3147 
   Size:       1951     535     467     150      44

  Fragments arranged by size: 

                1951     535     467     150      44
 
MspA1I CmG'CkG

Cuts at:      0      42      72      81     482     590     771    1641    1968 
   Size:         42      30       9     401     108     181     870     327

Cuts at:   1968    2543    2841    2922    2953    3147 
   Size:        575     298      81      31     194

  Fragments arranged by size: 

                 870     575     401     327     298     194     181     108
                  81      42      31      30       9
 
NaeI GCC'GGC

Cuts at:      0    1119    3147 
   Size:       1119    2028
 
NarI GG'CG_CC

Cuts at:      0      60    2060    3147 
   Size:         60    2000    1087

  Fragments arranged by size: 

                2000    1087      60
 
NcoI C'CATG_G

Cuts at:      0     444    3104    3147 
   Size:        444    2660      43

  Fragments arranged by size: 

                2660     444      43
 
NdeI CA'TA_TG

Cuts at:      0    3093    3147 
   Size:       3093      54
 
NgoMIV G'CCGG_C

Cuts at:      0    1117    3147 
   Size:       1117    2030
 
NspI r_CATG'y

Cuts at:      0     467    3147 
   Size:        467    2680
 
PasI CC'CwG_GG

Cuts at:      0     110    1194    2128    3147 
   Size:        110    1084     934    1019

  Fragments arranged by size: 

                1084    1019     934     110
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2046    2228    2556    3147 
   Size:       2046     182     328     591

  Fragments arranged by size: 

                2046     591     328     182
 
PfoI T'CCnGG_A

Cuts at:      0     359    2261    3147 
   Size:        359    1902     886

  Fragments arranged by size: 

                1902     886     359
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2374    2406    3147 
   Size:       2374      32     741

  Fragments arranged by size: 

                2374     741      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1200    1430    2460    2467    2713    3147 
   Size:       1200     230    1030       7     246     434

  Fragments arranged by size: 

                1200    1030     434     246     230       7
 
PspOMI G'GGCC_C

Cuts at:      0      51     160    1290    2770    3147 
   Size:         51     109    1130    1480     377

  Fragments arranged by size: 

                1480    1130     377     109      51
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     880     912    1981    2013    3147 
   Size:        880      32    1069      32    1134

  Fragments arranged by size: 

                1134    1069     880      32      32
 
PstI C_TGCA'G

Cuts at:      0     275     776    1109    1331    1540    1967    2804    2867 
   Size:        275     501     333     222     209     427     837      63

Cuts at:   2867    3147 
   Size:        280

  Fragments arranged by size: 

                 837     501     427     333     280     275     222     209
                  63
 
PvuII CAG'CTG

Cuts at:      0      42     482     590     771    1641    1968    2543    2922 
   Size:         42     440     108     181     870     327     575     379

Cuts at:   2922    2953    3147 
   Size:         31     194

  Fragments arranged by size: 

                 870     575     440     379     327     194     181     108
                  42      31
 
SacI G_AGCT'C

Cuts at:      0    2495    2768    3147 
   Size:       2495     273     379

  Fragments arranged by size: 

                2495     379     273
 
ScaI AGT'ACT

Cuts at:      0     152    2107    3147 
   Size:        152    1955    1040

  Fragments arranged by size: 

                1955    1040     152
 
SexAI A'CCwGG_T

Cuts at:      0     755    3147 
   Size:        755    2392
 
SfcI C'TryA_G

Cuts at:      0     271     772    1105    1327    1536    1963    2800    2863 
   Size:        271     501     333     222     209     427     837      63

Cuts at:   2863    3147 
   Size:        284

  Fragments arranged by size: 

                 837     501     427     333     284     271     222     209
                  63
 
SfoI GGC'GCC

Cuts at:      0      61    2061    3147 
   Size:         61    2000    1086

  Fragments arranged by size: 

                2000    1086      61
 
SmaI CCC'GGG

Cuts at:      0     167    3147 
   Size:        167    2980
 
SmlI C'TyrA_G

Cuts at:      0    1739    3121    3147 
   Size:       1739    1382      26

  Fragments arranged by size: 

                1739    1382      26
 
SphI G_CATG'C

Cuts at:      0     467    3147 
   Size:        467    2680
 
StyI C'CwwG_G

Cuts at:      0     242     298     444     615     813    1832    2502    2966 
   Size:        242      56     146     171     198    1019     670     464

Cuts at:   2966    3036    3104    3147 
   Size:         70      68      43

  Fragments arranged by size: 

                1019     670     464     242     198     171     146      70
                  68      56      43
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     123     255    1127    1993    2038    2141    3147 
   Size:        123     132     872     866      45     103    1006

  Fragments arranged by size: 

                1006     872     866     132     123     103      45
 
TatI w'GTAC_w

Cuts at:      0     150     404     476    2105    2879    3147 
   Size:        150     254      72    1629     774     268

  Fragments arranged by size: 

                1629     774     268     254     150      72
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     173     205     809     841    2076    2108    2728    2760 
   Size:        173      32     604      32    1235      32     620      32

Cuts at:   2760    3147 
   Size:        387

  Fragments arranged by size: 

                1235     620     604     387     173      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0     217    3147 
   Size:        217    2930
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     479    1162    2423    3147 
   Size:        479     683    1261     724

  Fragments arranged by size: 

                1261     724     683     479
 
XhoI C'TCGA_G

Cuts at:      0    1739    3147 
   Size:       1739    1408
 
XmaI C'CCGG_G

Cuts at:      0     165    3147 
   Size:        165    2982
 
ZraI GAC'GTC

Cuts at:      0    1002    3147 
   Size:       1002    2145
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI   DraIII     DrdI     EaeI     EagI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I  HindIII     KasI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NspI     PasI    PflMI     PfoI     PpiI    PpuMI   PspOMI
     PsrI     PstI    PvuII     SacI     ScaI    SexAI     SfcI     SfoI
     SmaI     SmlI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AscI     AseI    AsiSI
     BaeI    BfrBI     BmtI    BsaAI    BsaWI    BsiWI    BsmBI    BspEI
    BspHI   BssHII    BssSI   BstAPI    BstBI    BstXI     ClaI    CspCI
     DraI     EarI     EciI     FseI     FspI    FspAI   HincII     HpaI
     KpnI     MfeI     MluI     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI     PmlI    PshAI     PsiI    PspXI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     XbaI     XmnI
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