Restriction map for
pFN21AB5098
(Linear) (Six-base) MAPSORT of: KIBB5098.seq Check: 7687 from: 1 to: 3474
KIBB5098.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2697 3474
Size: 2697 777
AccI GT'mk_AC
Cuts at: 0 1634 3474
Size: 1634 1840
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1036 1416 2565 3474
Size: 1036 380 1149 909
Fragments arranged by size:
1149 1036 909 380
AflIII A'CryG_T
Cuts at: 0 1953 3474
Size: 1953 1521
AgeI A'CCGG_T
Cuts at: 0 1261 3474
Size: 1261 2213
AleI CACnn'nnGTG
Cuts at: 0 1516 3262 3474
Size: 1516 1746 212
Fragments arranged by size:
1746 1516 212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1280 1900 1984 3474
Size: 1280 620 84 1490
Fragments arranged by size:
1490 1280 620 84
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1834 1866 3474
Size: 1834 32 1608
Fragments arranged by size:
1834 1608 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1923 2352 3474
Size: 1923 429 1122
Fragments arranged by size:
1923 1122 429
ApaLI G'TGCA_C
Cuts at: 0 2710 3474
Size: 2710 764
AseI AT'TA_AT
Cuts at: 0 2789 3474
Size: 2789 685
AvaI C'yCGr_G
Cuts at: 0 112 146 178 571 796 1846 2375 2841
Size: 112 34 32 393 225 1050 529 466
Cuts at: 2841 2883 3295 3319 3367 3474
Size: 42 412 24 48 107
Fragments arranged by size:
1050 529 466 412 393 225 112 107
48 42 34 32 24
AvrII C'CTAG_G
Cuts at: 0 3129 3474
Size: 3129 345
BamHI G'GATC_C
Cuts at: 0 1868 3474
Size: 1868 1606
BanI G'GyrC_C
Cuts at: 0 18 68 312 597 752 1264 1688 1992
Size: 18 50 244 285 155 512 424 304
Cuts at: 1992 3107 3474
Size: 1115 367
Fragments arranged by size:
1115 512 424 367 304 285 244 155
50 18
BanII G_rGCy'C
Cuts at: 0 146 185 818 1100 1532 1780 1847 2041
Size: 146 39 633 282 432 248 67 194
Cuts at: 2041 2486 2612 3200 3296 3320 3474
Size: 445 126 588 96 24 154
Fragments arranged by size:
633 588 445 432 282 248 194 154
146 126 96 67 39 24
BbeI G_GCGC'C
Cuts at: 0 22 72 601 756 3111 3474
Size: 22 50 529 155 2355 363
Fragments arranged by size:
2355 529 363 155 50 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 2809 3474
Size: 2809 665
BbvCI CC'TCA_GC
Cuts at: 0 2135 3474
Size: 2135 1339
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1294 1328 2514 2548 3474
Size: 1294 34 1186 34 926
Fragments arranged by size:
1294 1186 926 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2229 2316 3474
Size: 2229 87 1158
Fragments arranged by size:
2229 1158 87
BglII A'GATC_T
Cuts at: 0 1793 1808 3474
Size: 1793 15 1666
Fragments arranged by size:
1793 1666 15
BlpI GC'TnA_GC
Cuts at: 0 28 983 3474
Size: 28 955 2491
Fragments arranged by size:
2491 955 28
Bme1580I G_kGCm'C
Cuts at: 0 61 317 1236 1744 1997 2714 3093 3474
Size: 61 256 919 508 253 717 379 381
Fragments arranged by size:
919 717 508 381 379 256 253 61
BmgBI CAC'GTC
Cuts at: 0 159 3474
Size: 159 3315
BmrI ACTGGGnnnn_n'
Cuts at: 0 1317 1846 2064 3327 3474
Size: 1317 529 218 1263 147
Fragments arranged by size:
1317 1263 529 218 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 556 1374 1624 2402 2626 3474
Size: 83 473 818 250 778 224 848
Fragments arranged by size:
848 818 778 473 250 224 83
Bpu10I CC'TnA_GC
Cuts at: 0 140 665 2135 3181 3474
Size: 140 525 1470 1046 293
Fragments arranged by size:
1470 1046 525 293 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1774 3474
Size: 1774 1700
BsaI GGTCTCn'nnnn_
Cuts at: 0 628 673 1048 2403 3244 3322 3346 3439
Size: 628 45 375 1355 841 78 24 93
Cuts at: 3439 3474
Size: 35
Fragments arranged by size:
1355 841 628 375 93 78 45 35
24
BsaAI yAC'GTr
Cuts at: 0 918 1954 3474
Size: 918 1036 1520
Fragments arranged by size:
1520 1036 918
BsaHI Gr'CG_yC
Cuts at: 0 19 69 598 753 1132 1367 3048 3063
Size: 19 50 529 155 379 235 1681 15
Cuts at: 3063 3108 3474
Size: 45 366
Fragments arranged by size:
1681 529 379 366 235 155 50 45
19 15
BsaWI w'CCGG_w
Cuts at: 0 258 1261 2435 3474
Size: 258 1003 1174 1039
Fragments arranged by size:
1174 1039 1003 258
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1111 1141 1834 1864 2383 2413 3132 3162
Size: 1111 30 693 30 519 30 719 30
Cuts at: 3162 3283 3307 3313 3331 3337 3361 3474
Size: 121 24 6 18 6 24 113
Fragments arranged by size:
1111 719 693 519 121 113 30 30
30 30 24 24 18 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 589 646 688 691 804 838 865
Size: 118 471 57 42 3 113 34 27
Cuts at: 865 950 1024 1066 1126 1135 1138 1197 1750
Size: 85 74 42 60 9 3 59 553
Cuts at: 1750 2092 2152 3397 3400 3403 3406 3415 3418
Size: 342 60 1245 3 3 3 9 3
Cuts at: 3418 3474
Size: 56
Fragments arranged by size:
1245 553 471 342 118 113 85 74
60 60 59 57 56 42 42 34
27 9 9 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 559 840 1611 1864 1887 2061 2271 3230
Size: 559 281 771 253 23 174 210 959
Cuts at: 3230 3356 3474
Size: 126 118
Fragments arranged by size:
959 771 559 281 253 210 174 126
118 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1990 3474
Size: 1990 1484
BsiEI CG_ry'CG
Cuts at: 0 8 245 1245 3474
Size: 8 237 1000 2229
Fragments arranged by size:
2229 1000 237 8
BsiHKAI G_wGCw'C
Cuts at: 0 659 1532 1621 1780 2126 2612 2714 3187
Size: 659 873 89 159 346 486 102 473
Cuts at: 3187 3474
Size: 287
Fragments arranged by size:
873 659 486 473 346 287 159 102
89
BsmI GAATG_Cn'
Cuts at: 0 3424 3474
Size: 3424 50
Bsp1286I G_dGCh'C
Cuts at: 0 61 146 185 317 659 818 1100 1236
Size: 61 85 39 132 342 159 282 136
Cuts at: 1236 1532 1621 1744 1780 1847 1997 2041 2126
Size: 296 89 123 36 67 150 44 85
Cuts at: 2126 2486 2612 2714 3093 3187 3200 3296 3320
Size: 360 126 102 379 94 13 96 24
Cuts at: 3320 3474
Size: 154
Fragments arranged by size:
379 360 342 296 282 159 154 150
136 132 126 123 102 96 94 89
85 85 67 61 44 39 36 24
13
BspEI T'CCGG_A
Cuts at: 0 258 3474
Size: 258 3216
BspHI T'CATG_A
Cuts at: 0 1718 2643 3474
Size: 1718 925 831
Fragments arranged by size:
1718 925 831
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2597 2697 2905 3474
Size: 2597 100 208 569
Fragments arranged by size:
2597 569 208 100
BsrBI CCG'CTC
Cuts at: 0 801 2298 3474
Size: 801 1497 1176
Fragments arranged by size:
1497 1176 801
BsrDI GCAATG_nn'
Cuts at: 0 1443 2240 2756 2907 3474
Size: 1443 797 516 151 567
Fragments arranged by size:
1443 797 567 516 151
BsrFI r'CCGG_y
Cuts at: 0 1244 1261 1539 2518 2996 3474
Size: 1244 17 278 979 478 478
Fragments arranged by size:
1244 979 478 478 278 17
BsrGI T'GTAC_A
Cuts at: 0 1460 3474
Size: 1460 2014
BssSI C'ACGA_G
Cuts at: 0 1781 3474
Size: 1781 1693
BstAPI GCAn_nnn'nTGC
Cuts at: 0 81 1592 3474
Size: 81 1511 1882
Fragments arranged by size:
1882 1511 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1167 1577 2604 3474
Size: 1167 410 1027 870
Fragments arranged by size:
1167 1027 870 410
BstYI r'GATC_y
Cuts at: 0 1196 1793 1808 1868 2109 3474
Size: 1196 597 15 60 241 1365
Fragments arranged by size:
1365 1196 597 241 60 15
Bsu36I CC'TnA_GG
Cuts at: 0 407 821 3474
Size: 407 414 2653
Fragments arranged by size:
2653 414 407
BtgI C'CryG_G
Cuts at: 0 190 1221 1802 1872 2213 2222 2363 2490
Size: 190 1031 581 70 341 9 141 127
Cuts at: 2490 2715 3474
Size: 225 759
Fragments arranged by size:
1031 759 581 341 225 190 141 127
70 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1955 3005 3474
Size: 1955 1050 469
Fragments arranged by size:
1955 1050 469
BtsI GCAGTG_nn'
Cuts at: 0 544 1076 1859 3474
Size: 544 532 783 1615
Fragments arranged by size:
1615 783 544 532
DraIII CAC_nnn'GTG
Cuts at: 0 1992 2799 2930 3144 3474
Size: 1992 807 131 214 330
Fragments arranged by size:
1992 807 330 214 131
EaeI y'GGCC_r
Cuts at: 0 5 232 242 541 808 1242 1664 2354
Size: 5 227 10 299 267 434 422 690
Cuts at: 2354 2981 3417 3474
Size: 627 436 57
Fragments arranged by size:
690 627 436 434 422 299 267 227
57 10 5
EagI C'GGCC_G
Cuts at: 0 5 242 1242 3474
Size: 5 237 1000 2232
Fragments arranged by size:
2232 1000 237 5
EarI CTCTTCn'nnn_
Cuts at: 0 496 995 3374 3392 3474
Size: 496 499 2379 18 82
Fragments arranged by size:
2379 499 496 82 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 953 3474
Size: 953 2521
EcoICRI GAG'CTC
Cuts at: 0 1530 1778 2610 3474
Size: 1530 248 832 864
Fragments arranged by size:
1530 864 832 248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 556 1036 1374 1416 1624 2402 2565
Size: 83 473 480 338 42 208 778 163
Cuts at: 2565 2626 3474
Size: 61 848
Fragments arranged by size:
848 778 480 473 338 208 163 83
61 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1969 3474
Size: 1969 1505
EcoO109I rG'GnC_Cy
Cuts at: 0 165 304 335 506 831 1319 2151 2163
Size: 165 139 31 171 325 488 832 12
Cuts at: 2163 3133 3204 3474
Size: 970 71 270
Fragments arranged by size:
970 832 488 325 270 171 165 139
71 31 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 342 2280 3474
Size: 342 1938 1194
Fragments arranged by size:
1938 1194 342
FspI TGC'GCA
Cuts at: 0 1960 3474
Size: 1960 1514
HaeII r_GCGC'y
Cuts at: 0 22 72 601 756 990 2062 2689 2891
Size: 22 50 529 155 234 1072 627 202
Cuts at: 2891 3111 3474
Size: 220 363
Fragments arranged by size:
1072 627 529 363 234 220 202 155
50 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 849 881 2101 2133 2383 2415 3474
Size: 849 32 1220 32 250 32 1059
Fragments arranged by size:
1220 1059 849 250 32 32 32
HincII GTy'rAC
Cuts at: 0 2538 2907 3474
Size: 2538 369 567
Fragments arranged by size:
2538 567 369
KasI G'GCGC_C
Cuts at: 0 18 68 597 752 3107 3474
Size: 18 50 529 155 2355 367
Fragments arranged by size:
2355 529 367 155 50 18
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 2978 3221 3317 3474
Size: 1097 1881 243 96 157
Fragments arranged by size:
1881 1097 243 157 96
MscI TGG'CCA
Cuts at: 0 543 810 1666 2983 3419 3474
Size: 543 267 856 1317 436 55
Fragments arranged by size:
1317 856 543 436 267 55
MslI CAynn'nnrTG
Cuts at: 0 1165 1516 1723 2648 2698 2776 2983 3262
Size: 1165 351 207 925 50 78 207 279
Cuts at: 3262 3474
Size: 212
Fragments arranged by size:
1165 925 351 279 212 207 207 78
50
MspA1I CmG'CkG
Cuts at: 0 32 192 891 2349 2492 2717 3226 3360
Size: 32 160 699 1458 143 225 509 134
Cuts at: 3360 3474
Size: 114
Fragments arranged by size:
1458 699 509 225 160 143 134 114
32
NaeI GCC'GGC
Cuts at: 0 1246 3474
Size: 1246 2228
NarI GG'CG_CC
Cuts at: 0 19 69 598 753 3108 3474
Size: 19 50 529 155 2355 366
Fragments arranged by size:
2355 529 366 155 50 19
NcoI C'CATG_G
Cuts at: 0 1802 1872 2363 3474
Size: 1802 70 491 1111
Fragments arranged by size:
1802 1111 491 70
NgoMIV G'CCGG_C
Cuts at: 0 1244 3474
Size: 1244 2230
NspI r_CATG'y
Cuts at: 0 1257 1599 1710 3474
Size: 1257 342 111 1764
Fragments arranged by size:
1764 1257 342 111
PasI CC'CwG_GG
Cuts at: 0 1377 1822 3200 3474
Size: 1377 445 1378 274
Fragments arranged by size:
1378 1377 445 274
PflMI CCAn_nnn'nTGG
Cuts at: 0 1227 3474
Size: 1227 2247
PfoI T'CCnGG_A
Cuts at: 0 624 2105 2969 3474
Size: 624 1481 864 505
Fragments arranged by size:
1481 864 624 505
PmlI CAC'GTG
Cuts at: 0 1954 3474
Size: 1954 1520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1834 1866 3474
Size: 1834 32 1608
Fragments arranged by size:
1834 1608 32
PpuMI rG'GwC_Cy
Cuts at: 0 335 1319 2151 2163 3474
Size: 335 984 832 12 1311
Fragments arranged by size:
1311 984 832 335 12
PspXI vC'TCGA_Gb
Cuts at: 0 112 3474
Size: 112 3362
PstI C_TGCA'G
Cuts at: 0 3056 3474
Size: 3056 418
PvuII CAG'CTG
Cuts at: 0 32 2349 3360 3474
Size: 32 2317 1011 114
Fragments arranged by size:
2317 1011 114 32
RsrII CG'GwC_CG
Cuts at: 0 2561 3474
Size: 2561 913
SacI G_AGCT'C
Cuts at: 0 1532 1780 2612 3474
Size: 1532 248 832 862
Fragments arranged by size:
1532 862 832 248
SacII CC_GC'GG
Cuts at: 0 193 2493 2718 3474
Size: 193 2300 225 756
Fragments arranged by size:
2300 756 225 193
SanDI GG'GwC_CC
Cuts at: 0 335 2151 2163 3474
Size: 335 1816 12 1311
Fragments arranged by size:
1816 1311 335 12
SexAI A'CCwGG_T
Cuts at: 0 1988 3474
Size: 1988 1486
SfcI C'TryA_G
Cuts at: 0 1635 3052 3474
Size: 1635 1417 422
Fragments arranged by size:
1635 1417 422
SfoI GGC'GCC
Cuts at: 0 20 70 599 754 3109 3474
Size: 20 50 529 155 2355 365
Fragments arranged by size:
2355 529 365 155 50 20
SgrAI Cr'CCGG_yG
Cuts at: 0 2518 3474
Size: 2518 956
SmaI CCC'GGG
Cuts at: 0 148 2377 2843 3474
Size: 148 2229 466 631
Fragments arranged by size:
2229 631 466 148
SmlI C'TyrA_G
Cuts at: 0 112 1789 3474
Size: 112 1677 1685
Fragments arranged by size:
1685 1677 112
SphI G_CATG'C
Cuts at: 0 1599 3474
Size: 1599 1875
StuI AGG'CCT
Cuts at: 0 1787 3474
Size: 1787 1687
StyI C'CwwG_G
Cuts at: 0 308 317 326 374 392 425 440 473
Size: 308 9 9 48 18 33 15 33
Cuts at: 473 1046 1802 1872 2363 3129 3308 3474
Size: 573 756 70 491 766 179 166
Fragments arranged by size:
766 756 573 491 308 179 166 70
48 33 33 18 15 9 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 103 387 453 486 697 1072 1390 1443
Size: 103 284 66 33 211 375 318 53
Cuts at: 1443 2494 3298 3474
Size: 1051 804 176
Fragments arranged by size:
1051 804 375 318 284 211 176 103
66 53 33
TatI w'GTAC_w
Cuts at: 0 743 1460 3474
Size: 743 717 2014
Fragments arranged by size:
2014 743 717
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1111 1143 1938 1970 3132 3164 3474
Size: 1111 32 795 32 1162 32 310
Fragments arranged by size:
1162 1111 795 310 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2395 3474
Size: 2395 1079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 324 1422 1674 3474
Size: 324 1098 252 1800
Fragments arranged by size:
1800 1098 324 252
XhoI C'TCGA_G
Cuts at: 0 112 3474
Size: 112 3362
XmaI C'CCGG_G
Cuts at: 0 146 2375 2841 3474
Size: 146 2229 466 633
Fragments arranged by size:
2229 633 466 146
XmnI GAAnn'nnTTC
Cuts at: 0 2263 3474
Size: 2263 1211
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AleI AlfI AloI
AlwNI ApaLI AseI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PfoI PmlI PpiI PpuMI PspXI PstI
PvuII RsrII SacI SacII SanDI SexAI SfcI SfoI
SgrAI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AhdI ApaI ApoI
AscI AsiSI BaeI BclI BfrBI BglI BmtI BplI
BsaBI BsiWI BsmBI BssHII BstBI BstEII BstZ17I ClaI
CspCI DraI DrdI EcoRI EcoRV FseI FspAI HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PshAI PsiI PspOMI PsrI
PvuI SalI SapI SbfI ScaI SfiI SgfI SnaBI
SpeI SrfI SspI SwaI XbaI ZraI