Restriction map for
pFN21AB5093
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccI AccIII AgeI
AhdI AscI Asp718I AspEI AspI
AviII BalI BamHI BbrPI BcgI
BsaAI BsaWI BseAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Cfr10I ClaI CpoI DrdI DsaI
Eam1105I EcoO65I EcoRI FseI FspI
HgaI KpnI KspI MluI MluNI
MroI MscI NaeI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AclI 2124 AACGTT AA^CGTT
AflII 819 CTTAAG C^TTAAG
AflIII 2451 ACGTGT A^C[GA][CT]GT
Alw44I 508 GTGCAC G^TGCAC
AlwI 2470 GGATC GGATC(4/5)
Aor51HI 180 AGCGCT AGC^GCT
ApaLI 508 GTGCAC G^TGCAC
AspHI 508 GTGCAC G[AT]GC[AT]^C
AvrII 2010 CCTAGG C^CTAGG
BbsI 2428 GAAGAC GAAGAC(2/6)
BbvCI 516 CCTCAGC CCTCAGC(-5/-2)
BciVI 572 GTATCC GTATCC(6/5)
BclI 2312 TGATCA T^GATCA
BfrI 819 CTTAAG C^TTAAG
BlnI 2010 CCTAGG C^CTAGG
BlpI 162 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 162 GCTCAGC GC^T[ACGT]AGC
BpuAI 2428 GAAGAC GAAGAC(2/6)
BsaBI 805 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1881 CTCCTC GAGGAG(10/8)
BsiHKAI 508 GTGCAC G[AT]GC[AT]^C
BsmFI 430 GTCCC GGGAC(10/14)
BspMI 1667 ACCTGC ACCTGC(4/8)
BsrDI 2372 CATTGC GCAATG(2/0)
CelII 162 GCTCAGC GC^T[ACGT]AGC
DraII 2204 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1474 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 180 AGCGCT AGC^GCT
EcoO109I 2204 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 224 ATGCAT ATGCA^T
FbaI 2312 TGATCA T^GATCA
HindIII 1929 AAGCTT A^AGCTT
HpaI 1369 GTTAAC GTT^AAC
MamI 805 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 832 CATTTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1394 CATATG CA^TATG
NheI 1911 GCTAGC G^CTAGC
NsiI 224 ATGCAT ATGCA^T
PpuMI 2204 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2124 AACGTT AA^CGTT
PstI 96 CTGCAG CTGCA^G
SexAI 2762 ACCAGGT A^CC[AT]GGT
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# TWO CUT ENZYMES ( 55 enzymes) #
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AccII 69 CGCG CG^CG
87 CGCG
AcyI 72 GGCGCC G[GA]^CG[CT]C
90 GGCGCC
ApaI 6 GGGCCC GGGCC^C
58 GGGCCC
AseI 1905 ATTAAT AT^TAAT
2157 ATTAAT
AsnI 1905 ATTAAT AT^TAAT
2157 ATTAAT
Asp700I 367 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2584 GAATCGTTTC
AvaI 154 CCCGAG C^[CT]CG[GA]G
433 CCCGAG
BbeI 72 GGCGCC GGCGC^C
90 GGCGCC
BglI 153 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1996 GCCCTTTTGGC
BglII 563 AGATCT A^GATCT
1182 AGATCT
BsaHI 72 GGCGCC G[GA]^CG[CT]C
90 GGCGCC
BsaI 682 GAGACC GGTCTC(1/5)
1067 GAGACC
BsgI 925 GTGCAG GTGCAG(16/14)
2767 GTGCAG
BsoBI 154 CCCGAG C^[CT]CG[GA]G
433 CCCGAG
Bsp1407I 1564 TGTACA T^GTACA
1699 TGTACA
BsrGI 1564 TGTACA T^GTACA
1699 TGTACA
BstAPI 393 GCAGTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1844 GCAGTGGCTGC
BstUI 69 CGCG CG^CG
87 CGCG
BstYI 563 AGATCT [GA]^GATC[CT]
1182 AGATCT
DraI 1443 TTTAAA TTT^AAA
2422 TTTAAA
EagI 53 CGGCCG C^GGCCG
146 CGGCCG
EarI 250 GAAGAG CTCTTC(1/4)
1962 GAAGAG
EclXI 53 CGGCCG C^GGCCG
146 CGGCCG
Eco52I 53 CGGCCG C^GGCCG
146 CGGCCG
EcoNI 1285 CCTACGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1570 CCTTCAACAGG
EcoRV 187 GATATC GAT^ATC
1164 GATATC
EcoT14I 2010 CCTAGG C^C[AT][AT]GG
2496 CCAAGG
FauI 9 CCCGC CCCGC(4/6)
265 GCGGG
Hin1I 72 GGCGCC G[GA]^CG[CT]C
90 GGCGCC
HincII 650 GTCAAC GT[CT]^[GA]AC
1369 GTTAAC
HindII 650 GTCAAC GT[CT]^[GA]AC
1369 GTTAAC
KasI 72 GGCGCC G^GCGCC
90 GGCGCC
Ksp632I 250 GAAGAG CTCTTC(1/4)
1962 GAAGAG
MaeII 2125 ACGT A^CGT
2451 ACGT
MfeI 259 CAATTG C^AATTG
2265 CAATTG
MflI 563 AGATCT [GA]^GATC[CT]
1182 AGATCT
MunI 259 CAATTG C^AATTG
2265 CAATTG
MvnI 69 CGCG CG^CG
87 CGCG
NarI 72 GGCGCC GG^CGCC
90 GGCGCC
NspI 2241 GCATGT [GA]CATG^[CT]
2537 GCATGT
PleI 1429 GACTC GAGTC(4/5)
648 GAGTC
PshBI 1905 ATTAAT AT^TAAT
2157 ATTAAT
PvuII 99 CAGCTG CAG^CTG
2637 CAGCTG
SapI 250 GAAGAGC GCTCTTC(1/4)
1962 GAAGAGC
SfoI 72 GGCGCC GGC^GCC
90 GGCGCC
SmlI 567 CTCAAG C^T[CT][GA]AG
819 CTTAAG
SspBI 1564 TGTACA T^GTACA
1699 TGTACA
SspI 1598 AATATT AAT^ATT
2057 AATATT
StyI 2010 CCTAGG C^C[AT][AT]GG
2496 CCAAGG
TaqI 375 TCGA T^CGA
1922 TCGA
Tsp45I 1427 GTGAC ^GT[GC]AC
1516 GTCAC
TthHB8I 375 TCGA T^CGA
1922 TCGA
XbaI 246 TCTAGA T^CTAGA
1179 TCTAGA
XhoII 563 AGATCT [GA]^GATC[CT]
1182 AGATCT
XmnI 367 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2584 GAATCGTTTC
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
AocI ApoI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsiEI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII EaeI Eco81I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI TfiI Tru9I TseI
Tsp509I TspRI Van91I