(Circular) (Six-base) MAPSORT of: pFN21AB5093.seq Check: 1426 from: 1 to: 7558 pFN21AB5093 7558 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5368 278 Size: 53 83 186 936 3832 2468 Fragments arranged by size: 3832 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4901 1937 Size: 2964 4594 Acc65I G'GTAC_C Cuts at: 4879 4879 Size: 7558 AclI AA'CG_TT Cuts at: 1563 4130 5685 6058 1563 Size: 2567 1555 373 3063 Fragments arranged by size: 3063 2567 1555 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4469 5621 7005 7273 1632 Size: 2837 1152 1384 268 1917 Fragments arranged by size: 2837 1917 1384 1152 268 AfeI AGC'GCT Cuts at: 2187 2187 Size: 7558 AflII C'TTAA_G Cuts at: 820 1017 2824 820 Size: 197 1807 5554 Fragments arranged by size: 5554 1807 197 AflIII A'CryG_T Cuts at: 1203 4456 6457 7434 1203 Size: 3253 2001 977 1327 Fragments arranged by size: 3253 2001 1327 977 AgeI A'CCGG_T Cuts at: 6389 6389 Size: 7558 AhdI GACnn_n'nnGTC Cuts at: 6287 6287 Size: 7558 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7558 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7558 AlwNI CAG_nnn'CTG Cuts at: 2548 2957 3855 5301 6873 2548 Size: 409 898 1446 1572 3233 Fragments arranged by size: 3233 1572 1446 898 409 ApaI G_GGCC'C Cuts at: 2015 2067 2015 Size: 52 7506 ApaLI G'TGCA_C Cuts at: 2513 5615 6771 2513 Size: 3102 1156 3300 Fragments arranged by size: 3300 3102 1156 ApoI r'AATT_y Cuts at: 1493 2720 3111 3213 3445 3824 4867 5093 5245 Size: 1227 391 102 232 379 1043 226 152 Cuts at: 5245 7354 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1227 1043 391 379 232 226 152 102 AseI AT'TA_AT Cuts at: 160 1032 3911 4163 6112 160 Size: 872 2879 252 1949 1606 Fragments arranged by size: 2879 1949 1606 872 252 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7558 AvaI C'yCGr_G Cuts at: 1870 1943 2159 2438 4883 1870 Size: 73 216 279 2445 4545 Fragments arranged by size: 4545 2445 279 216 73 AvrII C'CTAG_G Cuts at: 4015 4015 Size: 7558 BamHI G'GATC_C Cuts at: 4888 5255 4888 Size: 367 7191 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 2095 2506 3152 4879 Size: 325 217 627 290 18 411 646 1727 Cuts at: 4879 6334 618 Size: 1455 1842 Fragments arranged by size: 1842 1727 1455 646 627 411 325 290 217 18 BanII G_rGCy'C Cuts at: 1391 2015 2067 2169 4877 1391 Size: 624 52 102 2708 4072 Fragments arranged by size: 4072 2708 624 102 52 BbeI G_GCGC'C Cuts at: 2081 2099 2081 Size: 18 7540 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4440 928 Size: 971 2541 4046 Fragments arranged by size: 4046 2541 971 BbvCI CC'TCA_GC Cuts at: 2522 2522 Size: 7558 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5749 5783 1903 Size: 34 50 34 3728 34 3678 Fragments arranged by size: 3728 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2588 5450 6660 2588 Size: 2862 1210 3486 Fragments arranged by size: 3486 2862 1210 BclI T'GATC_A Cuts at: 1548 4317 1548 Size: 2769 4789 BfrBI ATG'CAT Cuts at: 2231 2231 Size: 7558 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2164 4007 6169 Size: 107 122 71 631 605 492 1843 2162 Cuts at: 6169 136 Size: 1525 Fragments arranged by size: 2162 1843 1525 631 605 492 122 107 71 BglII A'GATC_T Cuts at: 2568 3187 7553 2568 Size: 619 4366 2573 Fragments arranged by size: 4366 2573 619 BlpI GC'TnA_GC Cuts at: 2168 4975 2168 Size: 2807 4751 Bme1580I G_kGCm'C Cuts at: 1790 2015 2067 2160 2517 5619 6775 1790 Size: 225 52 93 357 3102 1156 2573 Fragments arranged by size: 3102 2573 1156 357 225 93 52 BmgBI CAC'GTC Cuts at: 1318 4457 1318 Size: 3139 4419 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2361 3620 5289 Size: 416 53 83 399 24 944 1259 1669 Cuts at: 5289 6247 442 Size: 958 1753 Fragments arranged by size: 1753 1669 1259 958 944 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3920 1056 Size: 2864 4694 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4231 4231 Size: 7558 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 2705 3455 6218 1239 Size: 1085 381 750 2763 2579 Fragments arranged by size: 2763 2579 1085 750 381 Bpu10I CC'TnA_GC Cuts at: 2522 3038 3355 2522 Size: 516 317 6725 Fragments arranged by size: 6725 516 317 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2557 5683 6548 6846 2557 Size: 3126 865 298 3269 Fragments arranged by size: 3269 3126 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2681 3066 6221 882 Size: 617 1182 385 3155 2219 Fragments arranged by size: 3155 2219 1182 617 385 BsaAI yAC'GTr Cuts at: 493 1204 5349 493 Size: 711 4145 2702 Fragments arranged by size: 4145 2702 711 BsaBI GATnn'nnATC Cuts at: 2814 7552 2814 Size: 4738 2820 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2078 2096 5365 5747 Size: 53 83 186 936 545 18 3269 382 Cuts at: 5747 6382 275 Size: 635 1451 Fragments arranged by size: 3269 1451 936 635 545 382 186 83 53 18 BsaWI w'CCGG_w Cuts at: 5991 6389 6663 6810 7221 5991 Size: 398 274 147 411 6328 Fragments arranged by size: 6328 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7333 Fragments arranged by size: 7333 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3877 1186 Size: 2691 4867 BseYI C'CCAG_C Cuts at: 2066 3866 6761 7257 2066 Size: 1800 2895 496 2367 Fragments arranged by size: 2895 2367 1800 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2951 4793 1163 Size: 573 1215 1842 3928 Fragments arranged by size: 3928 1842 1215 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 2119 2154 5008 5769 6797 Size: 275 186 60 58 35 2854 761 1028 Cuts at: 6797 1540 Size: 2301 Fragments arranged by size: 2854 2301 1028 761 275 186 60 58 35 BsiHKAI G_wGCw'C Cuts at: 2517 5619 5704 6775 2517 Size: 3102 85 1071 3300 Fragments arranged by size: 3300 3102 1071 85 BsmI GAATG_Cn' Cuts at: 5069 5162 5069 Size: 93 7465 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2067 2160 2169 2517 4877 5619 Size: 399 225 52 93 9 348 2360 742 Cuts at: 5619 5704 6775 1391 Size: 85 1071 2174 Fragments arranged by size: 2360 2174 1071 742 399 348 225 93 85 52 9 BspEI T'CCGG_A Cuts at: 7221 7221 Size: 7558 BspHI T'CATG_A Cuts at: 5447 7177 5447 Size: 1730 5828 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3681 4913 844 Size: 2837 1232 3489 Fragments arranged by size: 3489 2837 1232 BsrBI CCG'CTC Cuts at: 1394 1823 5445 1394 Size: 429 3622 3507 Fragments arranged by size: 3622 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4376 6053 6227 65 Size: 1183 3128 1677 174 1396 Fragments arranged by size: 3128 1677 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6202 6389 1700 Size: 110 4392 187 2869 Fragments arranged by size: 4392 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3569 3704 96 Size: 3473 135 3950 Fragments arranged by size: 3950 3473 135 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7558 BssSI C'ACGA_G Cuts at: 5618 6630 5618 Size: 1012 6546 BstAPI GCAn_nnn'nTGC Cuts at: 794 2404 3855 794 Size: 1610 1451 4497 Fragments arranged by size: 4497 1610 1451 BstBI TT'CG_AA Cuts at: 6362 6362 Size: 7558 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7558 BstYI r'GATC_y Cuts at: 1972 2568 3187 4888 5255 5640 5657 7109 7195 Size: 596 619 1701 367 385 17 1452 86 Cuts at: 7195 7207 7553 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1701 1452 619 596 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2727 3380 4680 2727 Size: 653 1300 5605 Fragments arranged by size: 5605 1300 653 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7006 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6819 Fragments arranged by size: 6819 683 56 BtsI GCAGTG_nn' Cuts at: 3710 3856 4320 4665 5074 5868 5888 3710 Size: 146 464 345 409 794 20 5380 Fragments arranged by size: 5380 794 464 409 345 146 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7558 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4453 4488 529 Size: 35 3889 35 3599 Fragments arranged by size: 3889 3599 35 35 DraI TTT'AAA Cuts at: 3450 4429 4862 3450 Size: 979 433 6146 Fragments arranged by size: 6146 979 433 DraIII CAC_nnn'GTG Cuts at: 3484 3484 Size: 7558 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6565 809 Size: 507 5249 1802 Fragments arranged by size: 5249 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2151 4167 Size: 54 1425 50 275 18 228 93 2016 Cuts at: 4167 5005 5894 7523 8 Size: 838 889 1629 43 Fragments arranged by size: 2016 1629 1425 889 838 275 228 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2058 2151 5005 1537 Size: 275 246 93 2854 4090 Fragments arranged by size: 4090 2854 275 246 93 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 3962 5488 1353 Size: 897 1712 1526 3423 Fragments arranged by size: 3423 1712 1526 897 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6145 6519 6665 387 Size: 1064 138 4556 374 146 1280 Fragments arranged by size: 4556 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2705 3455 4469 5621 6218 7005 Size: 393 692 381 750 1014 1152 597 787 Cuts at: 7005 7273 1239 Size: 268 1524 Fragments arranged by size: 1524 1152 1014 787 750 692 597 393 381 268 EcoNI CCTnn'n_nnAGG Cuts at: 3294 3579 3294 Size: 285 7273 EcoO109I rG'GnC_Cy Cuts at: 4210 4210 Size: 7558 EcoRI G'AATT_C Cuts at: 4867 5245 4867 Size: 378 7180 EcoRV GAT'ATC Cuts at: 2194 3171 2194 Size: 977 6581 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2994 2994 Size: 7558 FspI TGC'GCA Cuts at: 6064 6064 Size: 7558 HaeII r_GCGC'y Cuts at: 1678 2081 2099 2175 2189 2549 6705 1678 Size: 403 18 76 14 360 4156 2531 Fragments arranged by size: 4156 2531 403 360 76 18 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2542 2574 3172 3204 3613 Size: 32 135 32 970 32 598 32 409 Cuts at: 3613 3645 3788 3820 3961 3993 6199 6231 6273 Size: 32 143 32 141 32 2206 32 42 Cuts at: 6273 6305 1373 Size: 32 2626 Fragments arranged by size: 2626 2206 970 598 409 143 141 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2657 3376 4902 5148 669 Size: 988 281 719 719 1526 246 3079 Fragments arranged by size: 3079 1526 988 719 719 281 246 HindIII A'AGCT_T Cuts at: 748 3934 748 Size: 3186 4372 HpaI GTT'AAC Cuts at: 3376 5148 3376 Size: 1772 5786 KasI G'GCGC_C Cuts at: 2077 2095 2077 Size: 18 7540 KpnI G_GTAC'C Cuts at: 4883 4883 Size: 7558 MfeI C'AATT_G Cuts at: 2264 4270 5157 2264 Size: 2006 887 4665 Fragments arranged by size: 4665 2006 887 MluI A'CGCG_T Cuts at: 7434 7434 Size: 7558 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4719 6672 6856 1305 Size: 3414 1953 184 2007 Fragments arranged by size: 3414 2007 1953 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5736 Fragments arranged by size: 5736 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2841 5516 5875 6034 518 Size: 648 1675 2675 359 159 2042 Fragments arranged by size: 2675 2042 1675 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2070 2106 4644 4974 5651 6799 7044 1606 Size: 464 36 2538 330 677 1148 245 2120 Fragments arranged by size: 2538 2120 1148 677 464 330 245 36 NarI GG'CG_CC Cuts at: 2078 2096 2078 Size: 18 7540 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7006 NdeI CA'TA_TG Cuts at: 387 3400 387 Size: 3013 4545 NheI G'CTAG_C Cuts at: 1052 3916 1052 Size: 2864 4694 NotI GC'GGCC_GC Cuts at: 5005 5005 Size: 7558 NsiI A_TGCA'T Cuts at: 2233 2233 Size: 7558 NspI r_CATG'y Cuts at: 1132 1229 4250 4546 4916 6461 1132 Size: 97 3021 296 370 1545 2229 Fragments arranged by size: 3021 2229 1545 370 296 97 PasI CC'CwG_GG Cuts at: 1348 2041 1348 Size: 693 6865 PciI A'CATG_T Cuts at: 6457 6457 Size: 7558 PflMI CCAn_nnn'nTGG Cuts at: 1108 2188 3103 3484 1108 Size: 1080 915 381 5182 Fragments arranged by size: 5182 1080 915 381 PfoI T'CCnGG_A Cuts at: 2135 3471 2135 Size: 1336 6222 PmeI GTTT'AAAC Cuts at: 4862 4862 Size: 7558 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5627 5659 7140 7172 5627 Size: 32 1481 32 6013 Fragments arranged by size: 6013 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4210 4210 Size: 7558 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7558 PsiI TTA'TAA Cuts at: 2706 5128 2706 Size: 2422 5136 PspOMI G'GGCC_C Cuts at: 2011 2063 2011 Size: 52 7506 PstI C_TGCA'G Cuts at: 830 2105 4910 830 Size: 1275 2805 3478 Fragments arranged by size: 3478 2805 1275 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7558 PvuII CAG'CTG Cuts at: 2106 4644 2106 Size: 2538 5020 SalI G'TCGA_C Cuts at: 1936 4900 1936 Size: 2964 4594 SapI GCTCTTCn'nnn_ Cuts at: 2250 3962 2250 Size: 1712 5846 SbfI CC_TGCA'GG Cuts at: 4910 4910 Size: 7558 ScaI AGT'ACT Cuts at: 5806 7518 5806 Size: 1712 5846 SexAI A'CCwGG_T Cuts at: 4767 4767 Size: 7558 SfcI C'TryA_G Cuts at: 826 1045 2101 2540 4023 4095 4906 6041 6722 Size: 219 1056 439 1483 72 811 1135 681 Cuts at: 6722 6913 826 Size: 191 1471 Fragments arranged by size: 1483 1471 1135 1056 811 681 439 219 191 72 SfoI GGC'GCC Cuts at: 2079 2097 2079 Size: 18 7540 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7558 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7558 SmaI CCC'GGG Cuts at: 1872 4885 1872 Size: 3013 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2572 2824 5662 6563 6825 820 Size: 197 926 629 252 2838 901 262 1553 Fragments arranged by size: 2838 1553 926 901 629 262 252 197 SnaBI TAC'GTA Cuts at: 493 5349 493 Size: 4856 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7558 SphI G_CATG'C Cuts at: 1132 4916 1132 Size: 3784 3774 SspI AAT'ATT Cuts at: 5 52 3605 4064 5482 5 Size: 47 3553 459 1418 2081 Fragments arranged by size: 3553 2081 1418 459 47 StuI AGG'CCT Cuts at: 1517 2303 3165 3300 4778 1517 Size: 786 862 135 1478 4297 Fragments arranged by size: 4297 1478 862 786 135 StyI C'CwwG_G Cuts at: 513 1065 4015 4501 4997 513 Size: 552 2950 486 496 3074 Fragments arranged by size: 3074 2950 552 496 486 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2195 2310 5585 5602 5755 5940 6385 Size: 1255 615 115 3275 17 153 185 445 Cuts at: 6385 325 Size: 1498 Fragments arranged by size: 3275 1498 1255 615 445 185 153 115 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3569 3704 4545 Size: 274 80 33 51 1443 1592 135 841 Cuts at: 4545 5804 7516 96 Size: 1259 1712 138 Fragments arranged by size: 1712 1592 1443 1259 841 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7518 7550 7518 Size: 32 7526 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7558 XbaI T'CTAG_A Cuts at: 2251 3184 4894 2251 Size: 933 1710 4915 Fragments arranged by size: 4915 1710 933 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7558 XmaI C'CCGG_G Cuts at: 1870 4883 1870 Size: 3013 4545 XmnI GAAnn'nnTTC Cuts at: 2376 4593 5687 2376 Size: 2217 1094 4247 Fragments arranged by size: 4247 2217 1094 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5366 276 Size: 53 83 186 936 3832 2468 Fragments arranged by size: 3832 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BsiWI BsmBI BstXI BstZ17I EcoICRI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI