(Circular) (Six-base) MAPSORT of: pFN21AB5090.seq Check: 8890 from: 1 to: 7012 pFN21AB5090 7012 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4822 278 Size: 53 83 186 936 3286 2468 Fragments arranged by size: 3286 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4355 1937 Size: 2418 4594 Acc65I G'GTAC_C Cuts at: 2043 4333 2043 Size: 2290 4722 AclI AA'CG_TT Cuts at: 1563 5139 5512 1563 Size: 3576 373 3063 Fragments arranged by size: 3576 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2258 2432 2813 4110 4139 5075 6459 6727 Size: 626 174 381 1297 29 936 1384 268 Cuts at: 6727 1632 Size: 1917 Fragments arranged by size: 1917 1384 1297 936 626 381 268 174 29 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6815 AflIII A'CryG_T Cuts at: 1203 3945 5911 6888 1203 Size: 2742 1966 977 1327 Fragments arranged by size: 2742 1966 1327 977 AgeI A'CCGG_T Cuts at: 5843 5843 Size: 7012 AhdI GACnn_n'nnGTC Cuts at: 5741 5741 Size: 7012 AleI CACnn'nnGTG Cuts at: 1166 2168 3179 3872 1166 Size: 1002 1011 693 4306 Fragments arranged by size: 4306 1011 1002 693 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2766 3011 3839 1184 Size: 1582 245 828 4357 Fragments arranged by size: 4357 1582 828 245 AlwNI CAG_nnn'CTG Cuts at: 2104 2366 2434 2611 3937 4106 4755 6327 2104 Size: 262 68 177 1326 169 649 1572 2789 Fragments arranged by size: 2789 1572 1326 649 262 177 169 68 ApaLI G'TGCA_C Cuts at: 5069 6225 5069 Size: 1156 5856 ApoI r'AATT_y Cuts at: 1493 3065 3152 3224 3647 4321 4547 4699 6808 Size: 1572 87 72 423 674 226 152 2109 Cuts at: 6808 1493 Size: 1697 Fragments arranged by size: 2109 1697 1572 674 423 226 152 87 72 AseI AT'TA_AT Cuts at: 160 1032 5566 160 Size: 872 4534 1606 Fragments arranged by size: 4534 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7012 AvaI C'yCGr_G Cuts at: 1870 1943 4337 1870 Size: 73 2394 4545 Fragments arranged by size: 4545 2394 73 BamHI G'GATC_C Cuts at: 3143 4342 4709 3143 Size: 1199 367 5446 Fragments arranged by size: 5446 1199 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2043 2325 3815 4333 5788 Size: 325 217 627 256 282 1490 518 1455 Cuts at: 5788 618 Size: 1842 Fragments arranged by size: 1842 1490 1455 627 518 325 282 256 217 BanII G_rGCy'C Cuts at: 1391 4331 1391 Size: 2940 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4127 928 Size: 971 2228 3813 Fragments arranged by size: 3813 2228 971 BbvCI CC'TCA_GC Cuts at: 4240 4240 Size: 7012 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5203 5237 1903 Size: 34 3266 34 3678 Fragments arranged by size: 3678 3266 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4904 6114 4904 Size: 1210 5802 BclI T'GATC_A Cuts at: 1548 3318 1548 Size: 1770 5242 BfrBI ATG'CAT Cuts at: 2998 2998 Size: 7012 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2369 5623 136 Size: 107 122 71 631 605 697 3254 1525 Fragments arranged by size: 3254 1525 697 631 605 122 107 71 BglII A'GATC_T Cuts at: 2563 7007 2563 Size: 4444 2568 BlpI GC'TnA_GC Cuts at: 4429 4429 Size: 7012 Bme1580I G_kGCm'C Cuts at: 1790 3170 5073 6229 1790 Size: 1380 1903 1156 2573 Fragments arranged by size: 2573 1903 1380 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7012 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4743 5701 442 Size: 416 53 83 399 24 3326 958 1753 Fragments arranged by size: 3326 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7012 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3391 4303 3391 Size: 912 6100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2456 2492 2876 2939 3795 5672 1239 Size: 830 387 36 384 63 856 1877 2579 Fragments arranged by size: 2579 1877 856 830 387 384 63 36 Bpu10I CC'TnA_GC Cuts at: 3527 4240 3527 Size: 713 6299 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2309 2525 3338 3817 5137 6002 6300 2309 Size: 216 813 479 1320 865 298 3021 Fragments arranged by size: 3021 1320 865 813 479 298 216 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3323 3362 5675 882 Size: 617 1824 39 2313 2219 Fragments arranged by size: 2313 2219 1824 617 39 BsaAI yAC'GTr Cuts at: 493 1204 2695 4803 493 Size: 711 1491 2108 2702 Fragments arranged by size: 2702 2108 1491 711 BsaBI GATnn'nnATC Cuts at: 7006 7006 Size: 7012 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3030 3428 3858 4819 Size: 53 83 186 936 1497 398 430 961 Cuts at: 4819 5201 5836 275 Size: 382 635 1451 Fragments arranged by size: 1497 1451 961 936 635 430 398 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2457 5445 5843 6117 6264 6675 2457 Size: 2988 398 274 147 411 2794 Fragments arranged by size: 2988 2794 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3793 3823 1178 Size: 30 165 30 2390 30 4367 Fragments arranged by size: 4367 2390 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2732 2873 2936 3320 3532 4058 1186 Size: 1546 141 63 384 212 526 4140 Fragments arranged by size: 4140 1546 526 384 212 141 63 BseYI C'CCAG_C Cuts at: 2373 6215 6711 2373 Size: 3842 496 2674 Fragments arranged by size: 3842 2674 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2489 2717 3296 1163 Size: 573 753 228 579 4879 Fragments arranged by size: 4879 753 579 573 228 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2912 4462 5223 6251 1540 Size: 275 186 911 1550 761 1028 2301 Fragments arranged by size: 2301 1550 1028 911 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3803 5073 5158 6229 3803 Size: 1270 85 1071 4586 Fragments arranged by size: 4586 1270 1071 85 BsmI GAATG_Cn' Cuts at: 4049 4523 4616 4049 Size: 474 93 6445 Fragments arranged by size: 6445 474 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3170 3803 4331 5073 5158 6229 1391 Size: 399 1380 633 528 742 85 1071 2174 Fragments arranged by size: 2174 1380 1071 742 633 528 399 85 BspEI T'CCGG_A Cuts at: 2457 6675 2457 Size: 4218 2794 BspHI T'CATG_A Cuts at: 4901 6631 4901 Size: 1730 5282 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2271 4160 4179 4367 844 Size: 1427 1889 19 188 3489 Fragments arranged by size: 3489 1889 1427 188 19 BsrBI CCG'CTC Cuts at: 1394 1823 2977 3781 3787 4899 1394 Size: 429 1154 804 6 1112 3507 Fragments arranged by size: 3507 1154 1112 804 429 6 BsrDI GCAATG_nn' Cuts at: 65 1248 5507 5681 65 Size: 1183 4259 174 1396 Fragments arranged by size: 4259 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2907 3006 3026 5656 5843 1700 Size: 110 1097 99 20 2630 187 2869 Fragments arranged by size: 2869 2630 1097 187 110 99 20 BsrGI T'GTAC_A Cuts at: 96 2899 96 Size: 2803 4209 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7012 BssSI C'ACGA_G Cuts at: 5072 6084 5072 Size: 1012 6000 BstAPI GCAn_nnn'nTGC Cuts at: 794 2366 2431 2602 794 Size: 1572 65 171 5204 Fragments arranged by size: 5204 1572 171 65 BstBI TT'CG_AA Cuts at: 5816 5816 Size: 7012 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7012 BstXI CCAn_nnnn'nTGG Cuts at: 3423 4000 3423 Size: 577 6435 BstYI r'GATC_y Cuts at: 1972 2473 2563 3143 3903 4342 4709 5094 5111 Size: 501 90 580 760 439 367 385 17 Cuts at: 5111 6563 6649 6661 7007 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 760 580 501 439 385 367 346 90 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3242 3242 Size: 7012 BtgI C'CryG_G Cuts at: 513 1065 2004 2409 3912 4308 513 Size: 552 939 405 1503 396 3217 Fragments arranged by size: 3217 1503 939 552 405 396 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2699 487 Size: 683 56 1473 4800 Fragments arranged by size: 4800 1473 683 56 BtsI GCAGTG_nn' Cuts at: 2364 2625 4528 5322 5342 2364 Size: 261 1903 794 20 4034 Fragments arranged by size: 4034 1903 794 261 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7012 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6977 DraI TTT'AAA Cuts at: 4316 4316 Size: 7012 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6019 809 Size: 507 4703 1802 Fragments arranged by size: 4703 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2909 3516 Size: 54 1425 50 275 18 312 767 607 Cuts at: 3516 4067 4459 5348 6977 8 Size: 551 392 889 1629 43 Fragments arranged by size: 1629 1425 889 767 607 551 392 312 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2909 4459 1537 Size: 275 1097 1550 4090 Fragments arranged by size: 4090 1550 1097 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2456 2729 4942 1353 Size: 1103 273 2213 3423 Fragments arranged by size: 3423 2213 1103 273 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5599 5973 6119 387 Size: 1064 138 4010 374 146 1280 Fragments arranged by size: 4010 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2258 2432 2456 2492 2813 2876 Size: 393 437 189 174 24 36 321 63 Cuts at: 2876 2939 3795 4110 4139 5075 5672 6459 6727 Size: 63 856 315 29 936 597 787 268 Cuts at: 6727 1239 Size: 1524 Fragments arranged by size: 1524 936 856 787 597 437 393 321 315 268 189 174 63 63 36 29 24 EcoO109I rG'GnC_Cy Cuts at: 2890 3523 3605 2890 Size: 633 82 6297 Fragments arranged by size: 6297 633 82 EcoRI G'AATT_C Cuts at: 3224 4321 4699 3224 Size: 1097 378 5537 Fragments arranged by size: 5537 1097 378 FspI TGC'GCA Cuts at: 5518 5518 Size: 7012 HaeII r_GCGC'y Cuts at: 1678 4107 6159 1678 Size: 2429 2052 2531 Fragments arranged by size: 2531 2429 2052 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2107 2139 2269 2301 3101 Size: 32 135 32 535 32 130 32 800 Cuts at: 3101 3133 3445 3477 3552 3584 5653 5685 5727 Size: 32 312 32 75 32 2069 32 42 Cuts at: 5727 5759 1373 Size: 32 2626 Fragments arranged by size: 2626 2069 800 535 312 135 130 75 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2840 4284 4356 4602 669 Size: 988 281 902 1444 72 246 3079 Fragments arranged by size: 3079 1444 988 902 281 246 72 HindIII A'AGCT_T Cuts at: 748 748 Size: 7012 HpaI GTT'AAC Cuts at: 4602 4602 Size: 7012 KpnI G_GTAC'C Cuts at: 2047 4337 2047 Size: 2290 4722 MfeI C'AATT_G Cuts at: 3277 3495 4611 3277 Size: 218 1116 5678 Fragments arranged by size: 5678 1116 218 MluI A'CGCG_T Cuts at: 6888 6888 Size: 7012 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3225 3423 6126 6310 1305 Size: 1920 198 2703 184 2007 Fragments arranged by size: 2703 2007 1920 198 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5190 Fragments arranged by size: 5190 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 2993 3179 3872 4970 5329 5488 Size: 648 1002 825 186 693 1098 359 159 Cuts at: 5488 518 Size: 2042 Fragments arranged by size: 2042 1098 1002 825 693 648 359 186 159 MspA1I CmG'CkG Cuts at: 1606 2611 3577 3891 3937 4428 5105 6253 6498 Size: 1005 966 314 46 491 677 1148 245 Cuts at: 6498 1606 Size: 2120 Fragments arranged by size: 2120 1148 1005 966 677 491 314 245 46 NcoI C'CATG_G Cuts at: 513 1065 2004 4308 513 Size: 552 939 2304 3217 Fragments arranged by size: 3217 2304 939 552 NdeI CA'TA_TG Cuts at: 387 2137 387 Size: 1750 5262 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7012 NotI GC'GGCC_GC Cuts at: 4459 4459 Size: 7012 NsiI A_TGCA'T Cuts at: 3000 3000 Size: 7012 NspI r_CATG'y Cuts at: 1132 1229 2162 2560 2992 3451 3949 4370 5915 Size: 97 933 398 432 459 498 421 1545 Cuts at: 5915 1132 Size: 2229 Fragments arranged by size: 2229 1545 933 498 459 432 421 398 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7012 PciI A'CATG_T Cuts at: 3945 5911 3945 Size: 1966 5046 PflMI CCAn_nnn'nTGG Cuts at: 1108 3438 3913 1108 Size: 2330 475 4207 Fragments arranged by size: 4207 2330 475 PfoI T'CCnGG_A Cuts at: 2508 3146 2508 Size: 638 6374 PmeI GTTT'AAAC Cuts at: 4316 4316 Size: 7012 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2607 2639 5081 5113 6594 6626 2607 Size: 32 2442 32 1481 32 2993 Fragments arranged by size: 2993 2442 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2890 3523 3605 2890 Size: 633 82 6297 Fragments arranged by size: 6297 633 82 PshAI GACnn'nnGTC Cuts at: 1613 3029 1613 Size: 1416 5596 PsiI TTA'TAA Cuts at: 4582 4582 Size: 7012 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3737 3769 3737 Size: 32 6980 PstI C_TGCA'G Cuts at: 830 2362 3223 4157 4364 830 Size: 1532 861 934 207 3478 Fragments arranged by size: 3478 1532 934 861 207 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7012 PvuII CAG'CTG Cuts at: 2611 3577 3937 2611 Size: 966 360 5686 Fragments arranged by size: 5686 966 360 SalI G'TCGA_C Cuts at: 1936 4354 1936 Size: 2418 4594 SanDI GG'GwC_CC Cuts at: 2890 2890 Size: 7012 SbfI CC_TGCA'GG Cuts at: 4364 4364 Size: 7012 ScaI AGT'ACT Cuts at: 5260 6972 5260 Size: 1712 5300 SexAI A'CCwGG_T Cuts at: 3607 3607 Size: 7012 SfcI C'TryA_G Cuts at: 826 1045 2024 2068 2351 2358 3219 4153 4360 Size: 219 979 44 283 7 861 934 207 Cuts at: 4360 5495 6176 6367 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 979 934 861 681 283 219 207 191 44 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7012 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7012 SmaI CCC'GGG Cuts at: 1872 4339 1872 Size: 2467 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2288 2540 3353 3796 5116 6017 Size: 197 926 345 252 813 443 1320 901 Cuts at: 6017 6279 820 Size: 262 1553 Fragments arranged by size: 1553 1320 926 901 813 443 345 262 252 197 SnaBI TAC'GTA Cuts at: 493 4803 493 Size: 4310 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7012 SphI G_CATG'C Cuts at: 1132 2992 4370 1132 Size: 1860 1378 3774 Fragments arranged by size: 3774 1860 1378 SspI AAT'ATT Cuts at: 5 52 4936 5 Size: 47 4884 2081 Fragments arranged by size: 4884 2081 47 StuI AGG'CCT Cuts at: 1517 3351 1517 Size: 1834 5178 StyI C'CwwG_G Cuts at: 513 1065 2004 2111 3346 4038 4308 4451 513 Size: 552 939 107 1235 692 270 143 3074 Fragments arranged by size: 3074 1235 939 692 552 270 143 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3347 3862 5039 5056 5209 5394 5839 Size: 1255 1767 515 1177 17 153 185 445 Cuts at: 5839 325 Size: 1498 Fragments arranged by size: 1767 1498 1255 1177 515 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2899 3726 5258 Size: 274 80 33 51 1443 922 827 1532 Cuts at: 5258 6970 96 Size: 1712 138 Fragments arranged by size: 1712 1532 1443 922 827 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3597 3629 3793 3825 6972 7004 3597 Size: 32 164 32 3147 32 3605 Fragments arranged by size: 3605 3147 164 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7012 XbaI T'CTAG_A Cuts at: 2476 4348 2476 Size: 1872 5140 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2407 3501 2407 Size: 1094 5918 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7012 XmaI C'CCGG_G Cuts at: 1870 4337 1870 Size: 2467 4545 XmnI GAAnn'nnTTC Cuts at: 5141 5141 Size: 7012 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4820 276 Size: 53 83 186 936 3286 2468 Fragments arranged by size: 3286 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI