(Circular) (Six-base) MAPSORT of: pFN21AB5089.seq Check: 544 from: 1 to: 8407 pFN21AB5089 8407 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2482 3419 5004 2482 Size: 937 1585 5885 Fragments arranged by size: 5885 1585 937 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6217 278 Size: 53 83 186 936 4681 2468 Fragments arranged by size: 4681 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5750 1937 Size: 3813 4594 Acc65I G'GTAC_C Cuts at: 4095 5728 4095 Size: 1633 6774 AclI AA'CG_TT Cuts at: 1563 6534 6907 1563 Size: 4971 373 3063 Fragments arranged by size: 4971 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2504 2552 6470 7854 8122 1632 Size: 872 48 3918 1384 268 1917 Fragments arranged by size: 3918 1917 1384 872 268 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8210 AflIII A'CryG_T Cuts at: 1203 3096 4254 7306 8283 1203 Size: 1893 1158 3052 977 1327 Fragments arranged by size: 3052 1893 1327 1158 977 AgeI A'CCGG_T Cuts at: 7238 7238 Size: 8407 AhdI GACnn_n'nnGTC Cuts at: 2112 2875 4690 7136 2112 Size: 763 1815 2446 3383 Fragments arranged by size: 3383 2446 1815 763 AleI CACnn'nnGTG Cuts at: 1166 2057 3161 3962 1166 Size: 891 1104 801 5611 Fragments arranged by size: 5611 1104 891 801 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8407 AlwNI CAG_nnn'CTG Cuts at: 2860 2971 3379 4216 4702 5128 5197 5476 6150 Size: 111 408 837 486 426 69 279 674 Cuts at: 6150 7722 2860 Size: 1572 3545 Fragments arranged by size: 3545 1572 837 674 486 426 408 279 111 69 ApaI G_GGCC'C Cuts at: 2174 4624 2174 Size: 2450 5957 ApaLI G'TGCA_C Cuts at: 6464 7620 6464 Size: 1156 7251 ApoI r'AATT_y Cuts at: 1493 2591 4415 5716 5942 6094 8203 1493 Size: 1098 1824 1301 226 152 2109 1697 Fragments arranged by size: 2109 1824 1697 1301 1098 226 152 AseI AT'TA_AT Cuts at: 160 1032 6961 160 Size: 872 5929 1606 Fragments arranged by size: 5929 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8407 AvaI C'yCGr_G Cuts at: 1870 1943 2773 5645 5732 1870 Size: 73 830 2872 87 4545 Fragments arranged by size: 4545 2872 830 87 73 AvrII C'CTAG_G Cuts at: 5038 5038 Size: 8407 BamHI G'GATC_C Cuts at: 2511 4431 5737 6104 2511 Size: 1920 1306 367 4814 Fragments arranged by size: 4814 1920 1306 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 2705 3163 3414 3956 Size: 325 217 627 225 693 458 251 542 Cuts at: 3956 4008 4095 4425 5016 5033 5658 5728 7183 Size: 52 87 330 591 17 625 70 1455 Cuts at: 7183 618 Size: 1842 Fragments arranged by size: 1842 1455 693 627 625 591 542 458 330 325 251 225 217 87 70 52 17 BanII G_rGCy'C Cuts at: 1391 2174 2847 3497 3513 3902 4344 4624 4875 Size: 783 673 650 16 389 442 280 251 Cuts at: 4875 5502 5726 1391 Size: 627 224 4072 Fragments arranged by size: 4072 783 673 650 627 442 389 280 251 224 16 BbeI G_GCGC'C Cuts at: 2016 2709 2016 Size: 693 7714 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 7436 BbvCI CC'TCA_GC Cuts at: 4799 5007 4799 Size: 208 8199 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3479 3513 3740 3774 6598 6632 1903 Size: 34 1542 34 227 34 2824 34 3678 Fragments arranged by size: 3678 2824 1542 227 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3401 4454 6299 7509 3401 Size: 1053 1845 1210 4299 Fragments arranged by size: 4299 1845 1210 1053 BclI T'GATC_A Cuts at: 1548 4581 1548 Size: 3033 5374 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2806 5519 5580 Size: 107 122 71 631 605 1134 2713 61 Cuts at: 5580 5596 7018 136 Size: 16 1422 1525 Fragments arranged by size: 2713 1525 1422 1134 631 605 122 107 71 61 16 BglII A'GATC_T Cuts at: 8402 8402 Size: 8407 BlpI GC'TnA_GC Cuts at: 3935 5824 3935 Size: 1889 6518 Bme1580I G_kGCm'C Cuts at: 1790 2174 3740 3959 4428 4624 5038 5661 6468 Size: 384 1566 219 469 196 414 623 807 Cuts at: 6468 7624 1790 Size: 1156 2573 Fragments arranged by size: 2573 1566 1156 807 623 469 414 384 219 196 BmgBI CAC'GTC Cuts at: 1318 3097 3178 4600 4677 1318 Size: 1779 81 1422 77 5048 Fragments arranged by size: 5048 1779 1422 81 77 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4684 6138 7096 Size: 416 53 83 399 24 3267 1454 958 Cuts at: 7096 442 Size: 1753 Fragments arranged by size: 3267 1753 1454 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8407 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2125 3532 4674 5211 2125 Size: 1407 1142 537 5321 Fragments arranged by size: 5321 1407 1142 537 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2095 2321 2717 2753 2882 3203 3485 3620 Size: 856 226 396 36 129 321 282 135 Cuts at: 3620 5153 5441 7067 1239 Size: 1533 288 1626 2579 Fragments arranged by size: 2579 1626 1533 856 396 321 288 282 226 135 129 36 Bpu10I CC'TnA_GC Cuts at: 3485 3560 4659 4799 4995 5007 5207 3485 Size: 75 1099 140 196 12 200 6685 Fragments arranged by size: 6685 1099 200 196 140 75 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3605 4088 6532 7397 7695 3605 Size: 483 2444 865 298 4317 Fragments arranged by size: 4317 2444 865 483 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2057 2099 2862 3537 3863 4713 4914 Size: 617 558 42 763 675 326 850 201 Cuts at: 4914 7070 882 Size: 2156 2219 Fragments arranged by size: 2219 2156 850 763 675 617 558 326 201 42 BsaAI yAC'GTr Cuts at: 493 1204 4243 6198 493 Size: 711 3039 1955 2702 Fragments arranged by size: 3039 2702 1955 711 BsaBI GATnn'nnATC Cuts at: 8401 8401 Size: 8407 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2013 2025 2706 6214 Size: 53 83 186 936 480 12 681 3508 Cuts at: 6214 6596 7231 275 Size: 382 635 1451 Fragments arranged by size: 3508 1451 936 681 635 480 382 186 83 53 12 BsaWI w'CCGG_w Cuts at: 2598 4428 4457 4902 6840 7238 7512 7659 8070 Size: 1830 29 445 1938 398 274 147 411 Cuts at: 8070 2598 Size: 2935 Fragments arranged by size: 2935 1938 1830 445 411 398 274 147 29 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3278 3308 1178 Size: 30 165 30 1875 30 6277 Fragments arranged by size: 6277 1875 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2318 2417 2927 2983 3134 3505 3587 Size: 931 201 99 510 56 151 371 82 Cuts at: 3587 3692 3710 3782 3961 4418 5438 5510 5705 Size: 105 18 72 179 457 1020 72 195 Cuts at: 5705 1186 Size: 3888 Fragments arranged by size: 3888 1020 931 510 457 371 201 195 179 151 105 99 82 72 72 56 18 BseYI C'CCAG_C Cuts at: 2016 2964 3029 3365 3422 3526 3536 3593 3987 Size: 948 65 336 57 104 10 57 394 Cuts at: 3987 4702 4733 5166 5459 5514 5578 5599 7610 Size: 715 31 433 293 55 64 21 2011 Cuts at: 7610 8106 2016 Size: 496 2317 Fragments arranged by size: 2317 2011 948 715 496 433 394 336 293 104 65 64 57 57 55 31 21 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2393 5141 1163 Size: 573 657 2748 4429 Fragments arranged by size: 4429 2748 657 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2675 3113 4127 5857 6618 7646 Size: 275 186 674 438 1014 1730 761 1028 Cuts at: 7646 1540 Size: 2301 Fragments arranged by size: 2301 1730 1028 1014 761 674 438 275 186 BsiHKAI G_wGCw'C Cuts at: 2954 3497 4875 6468 6553 7624 2954 Size: 543 1378 1593 85 1071 3737 Fragments arranged by size: 3737 1593 1378 1071 543 85 BsmI GAATG_Cn' Cuts at: 3763 5918 6011 3763 Size: 2155 93 6159 Fragments arranged by size: 6159 2155 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3300 4667 3300 Size: 1367 7040 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2174 2847 2954 3497 3513 3740 3902 Size: 399 384 673 107 543 16 227 162 Cuts at: 3902 3959 4344 4428 4624 4875 5038 5502 5661 Size: 57 385 84 196 251 163 464 159 Cuts at: 5661 5726 6468 6553 7624 1391 Size: 65 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 673 543 464 399 385 384 251 227 196 163 162 159 107 85 84 65 57 16 BspEI T'CCGG_A Cuts at: 4457 4902 8070 4457 Size: 445 3168 4794 Fragments arranged by size: 4794 3168 445 BspHI T'CATG_A Cuts at: 6296 8026 6296 Size: 1730 6677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2482 3419 3667 4107 4932 5004 5461 5762 Size: 1638 937 248 440 825 72 457 301 Cuts at: 5762 844 Size: 3489 Fragments arranged by size: 3489 1638 937 825 457 440 301 248 72 BsrBI CCG'CTC Cuts at: 1394 1823 2647 3294 4455 6294 1394 Size: 429 824 647 1161 1839 3507 Fragments arranged by size: 3507 1839 1161 824 647 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3189 3571 4389 6902 7076 65 Size: 1183 1941 382 818 2513 174 1396 Fragments arranged by size: 2513 1941 1396 1183 818 382 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2229 4510 4758 4908 5019 7051 7238 Size: 110 419 2281 248 150 111 2032 187 Cuts at: 7238 1700 Size: 2869 Fragments arranged by size: 2869 2281 2032 419 248 187 150 111 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8407 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8407 BssSI C'ACGA_G Cuts at: 6467 7479 6467 Size: 1012 7395 BstAPI GCAn_nnn'nTGC Cuts at: 794 5128 5476 794 Size: 4334 348 3725 Fragments arranged by size: 4334 3725 348 BstBI TT'CG_AA Cuts at: 7211 7211 Size: 8407 BstEII G'GTnAC_C Cuts at: 1184 2125 1184 Size: 941 7466 BstXI CCAn_nnnn'nTGG Cuts at: 2059 3880 4741 4864 5509 2059 Size: 1821 861 123 645 4957 Fragments arranged by size: 4957 1821 861 645 123 BstYI r'GATC_y Cuts at: 1972 2259 2511 2688 2944 4431 5292 5562 5737 Size: 287 252 177 256 1487 861 270 175 Cuts at: 5737 6104 6489 6506 7958 8044 8056 8402 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1487 1452 861 385 367 346 287 270 256 252 177 175 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3117 3117 Size: 8407 BtgI C'CryG_G Cuts at: 513 1065 2004 2055 2880 3139 3879 3903 513 Size: 552 939 51 825 259 740 24 5017 Fragments arranged by size: 5017 939 825 740 552 259 51 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4064 4813 487 Size: 683 56 2838 749 4081 Fragments arranged by size: 4081 2838 749 683 56 BtsI GCAGTG_nn' Cuts at: 3012 5923 6717 6737 3012 Size: 2911 794 20 4682 Fragments arranged by size: 4682 2911 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8407 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8372 DraI TTT'AAA Cuts at: 5711 5711 Size: 8407 DraIII CAC_nnn'GTG Cuts at: 2975 2975 Size: 8407 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2323 7414 809 Size: 507 1007 5091 1802 Fragments arranged by size: 5091 1802 1007 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2367 2672 3110 Size: 54 1425 50 275 18 537 305 438 Cuts at: 3110 3501 4124 4176 4632 4666 5027 5854 6743 Size: 391 623 52 456 34 361 827 889 Cuts at: 6743 8372 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 827 623 537 456 438 391 361 305 275 54 52 50 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 2672 3110 4124 5854 1537 Size: 275 860 438 1014 1730 4090 Fragments arranged by size: 4090 1730 1014 860 438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3687 3998 4332 5487 6337 1353 Size: 2334 311 334 1155 850 3423 Fragments arranged by size: 3423 2334 1155 850 334 311 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5375 5555 6994 7368 7514 387 Size: 1064 138 3786 180 1439 374 146 1280 Fragments arranged by size: 3786 1439 1280 1064 374 180 146 138 EcoICRI GAG'CTC Cuts at: 3495 4873 3495 Size: 1378 7029 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2321 2504 2552 2717 2753 2882 Size: 393 463 226 183 48 165 36 129 Cuts at: 2882 3203 3485 3620 5153 5441 6470 7067 7854 Size: 321 282 135 1533 288 1029 597 787 Cuts at: 7854 8122 1239 Size: 268 1524 Fragments arranged by size: 1533 1524 1029 787 597 463 393 321 288 282 268 226 183 165 135 129 48 36 EcoNI CCTnn'n_nnAGG Cuts at: 2246 4844 4994 5189 2246 Size: 2598 150 195 5464 Fragments arranged by size: 5464 2598 195 150 EcoO109I rG'GnC_Cy Cuts at: 2171 2251 2285 3033 3376 3628 3641 4576 4620 Size: 80 34 748 343 252 13 935 44 Cuts at: 4620 5473 2171 Size: 853 5105 Fragments arranged by size: 5105 935 853 748 343 252 80 44 34 13 EcoRI G'AATT_C Cuts at: 5716 6094 5716 Size: 378 8029 FspI TGC'GCA Cuts at: 2465 4937 6913 2465 Size: 2472 1976 3959 Fragments arranged by size: 3959 2472 1976 HaeII r_GCGC'y Cuts at: 1678 2016 2709 3447 5311 5673 7554 1678 Size: 338 693 738 1864 362 1881 2531 Fragments arranged by size: 2531 1881 1864 738 693 362 338 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2098 2130 2861 2893 3758 Size: 32 135 32 526 32 731 32 865 Cuts at: 3758 3790 3842 3874 4676 4708 5365 5397 7048 Size: 32 52 32 802 32 657 32 1651 Cuts at: 7048 7080 7122 7154 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1651 865 802 731 657 526 135 52 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3787 5404 5751 5997 669 Size: 988 281 1849 1617 347 246 3079 Fragments arranged by size: 3079 1849 1617 988 347 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8407 HpaI GTT'AAC Cuts at: 5997 5997 Size: 8407 KasI G'GCGC_C Cuts at: 2012 2705 2012 Size: 693 7714 KpnI G_GTAC'C Cuts at: 4099 5732 4099 Size: 1633 6774 MfeI C'AATT_G Cuts at: 6006 6006 Size: 8407 MluI A'CGCG_T Cuts at: 8283 8283 Size: 8407 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2022 2096 2489 3999 4245 7521 7705 1305 Size: 717 74 393 1510 246 3276 184 2007 Fragments arranged by size: 3276 2007 1510 717 393 246 184 74 MscI TGG'CCA Cuts at: 10 64 1489 1832 4178 4668 10 Size: 54 1425 343 2346 490 3749 Fragments arranged by size: 3749 2346 1425 490 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2183 2369 2957 3067 3161 3962 Size: 648 891 126 186 588 110 94 801 Cuts at: 3962 4241 6365 6724 6883 518 Size: 279 2124 359 159 2042 Fragments arranged by size: 2124 2042 891 801 648 588 359 279 186 159 126 110 94 MspA1I CmG'CkG Cuts at: 1606 3025 3081 3343 3526 3549 4092 4501 4630 Size: 1419 56 262 183 23 543 409 129 Cuts at: 4630 4702 4864 4951 5051 5170 5203 5440 5485 Size: 72 162 87 100 119 33 237 45 Cuts at: 5485 5518 5542 5655 5823 6500 7648 7893 1606 Size: 33 24 113 168 677 1148 245 2120 Fragments arranged by size: 2120 1419 1148 677 543 409 262 245 237 183 168 162 129 119 113 100 87 72 56 45 33 33 24 23 NaeI GCC'GGC Cuts at: 4512 4760 4910 5021 4512 Size: 248 150 111 7898 Fragments arranged by size: 7898 248 150 111 NarI GG'CG_CC Cuts at: 2013 2706 2013 Size: 693 7714 NcoI C'CATG_G Cuts at: 513 1065 2004 2880 3879 3903 513 Size: 552 939 876 999 24 5017 Fragments arranged by size: 5017 999 939 876 552 24 NdeI CA'TA_TG Cuts at: 387 387 Size: 8407 NgoMIV G'CCGG_C Cuts at: 4510 4758 4908 5019 4510 Size: 248 150 111 7898 Fragments arranged by size: 7898 248 150 111 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8407 NotI GC'GGCC_GC Cuts at: 5854 5854 Size: 8407 NspI r_CATG'y Cuts at: 1132 1229 3985 4258 5765 7310 1132 Size: 97 2756 273 1507 1545 2229 Fragments arranged by size: 2756 2229 1545 1507 273 97 PasI CC'CwG_GG Cuts at: 1348 4808 1348 Size: 3460 4947 PciI A'CATG_T Cuts at: 4254 7306 4254 Size: 3052 5355 PflMI CCAn_nnn'nTGG Cuts at: 1108 2394 1108 Size: 1286 7121 PfoI T'CCnGG_A Cuts at: 2262 2447 5295 2262 Size: 185 2848 5374 Fragments arranged by size: 5374 2848 185 PmeI GTTT'AAAC Cuts at: 5711 5711 Size: 8407 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6476 6508 7989 8021 6476 Size: 32 1481 32 6862 Fragments arranged by size: 6862 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2251 2285 3033 3376 5473 2251 Size: 34 748 343 2097 5185 Fragments arranged by size: 5185 2097 748 343 34 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8407 PsiI TTA'TAA Cuts at: 5977 5977 Size: 8407 PspOMI G'GGCC_C Cuts at: 2170 4620 2170 Size: 2450 5957 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5087 5119 5087 Size: 32 8375 PstI C_TGCA'G Cuts at: 830 2040 2493 5202 5289 5759 830 Size: 1210 453 2709 87 470 3478 Fragments arranged by size: 3478 2709 1210 470 453 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8407 PvuII CAG'CTG Cuts at: 3025 3526 3549 4630 4702 5485 5518 3025 Size: 501 23 1081 72 783 33 5914 Fragments arranged by size: 5914 1081 783 501 72 33 23 SacI G_AGCT'C Cuts at: 3497 4875 3497 Size: 1378 7029 SalI G'TCGA_C Cuts at: 1936 5749 1936 Size: 3813 4594 SanDI GG'GwC_CC Cuts at: 2285 2285 Size: 8407 SapI GCTCTTCn'nnn_ Cuts at: 3998 4332 3998 Size: 334 8073 SbfI CC_TGCA'GG Cuts at: 5759 5759 Size: 8407 ScaI AGT'ACT Cuts at: 3287 6655 8367 3287 Size: 3368 1712 3327 Fragments arranged by size: 3368 3327 1712 SexAI A'CCwGG_T Cuts at: 2121 2121 Size: 8407 SfcI C'TryA_G Cuts at: 826 1045 2036 2489 3726 5198 5285 5755 6890 Size: 219 991 453 1237 1472 87 470 1135 Cuts at: 6890 7571 7762 826 Size: 681 191 1471 Fragments arranged by size: 1472 1471 1237 1135 991 681 470 453 219 191 87 SfiI GGCCn_nnn'nGGCC Cuts at: 5519 5519 Size: 8407 SfoI GGC'GCC Cuts at: 2014 2707 2014 Size: 693 7714 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8407 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8407 SmaI CCC'GGG Cuts at: 1872 5647 5734 1872 Size: 3775 87 4545 Fragments arranged by size: 4545 3775 87 SmlI C'TyrA_G Cuts at: 820 1017 1943 3584 4103 6511 7412 7674 820 Size: 197 926 1641 519 2408 901 262 1553 Fragments arranged by size: 2408 1641 1553 926 901 519 262 197 SnaBI TAC'GTA Cuts at: 493 6198 493 Size: 5705 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8407 SphI G_CATG'C Cuts at: 1132 5765 1132 Size: 4633 3774 SspI AAT'ATT Cuts at: 5 52 6331 5 Size: 47 6279 2081 Fragments arranged by size: 6279 2081 47 StuI AGG'CCT Cuts at: 1517 3558 1517 Size: 2041 6366 StyI C'CwwG_G Cuts at: 513 1065 2004 2247 2388 2631 2709 2880 3873 Size: 552 939 243 141 243 78 171 993 Cuts at: 3873 3879 3903 3951 4356 5038 5846 513 Size: 6 24 48 405 682 808 3074 Fragments arranged by size: 3074 993 939 808 682 552 405 243 243 171 141 78 48 24 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3608 4703 6434 6451 6604 6789 7234 Size: 1255 2028 1095 1731 17 153 185 445 Cuts at: 7234 325 Size: 1498 Fragments arranged by size: 2028 1731 1498 1255 1095 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2160 3285 4077 Size: 274 80 33 51 1443 183 1125 792 Cuts at: 4077 6653 8365 96 Size: 2576 1712 138 Fragments arranged by size: 2576 1712 1443 1125 792 274 183 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8367 8399 8367 Size: 32 8375 Tth111I GACn'n_nGTC Cuts at: 1316 3037 1316 Size: 1721 6686 XbaI T'CTAG_A Cuts at: 5743 5743 Size: 8407 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2119 2182 3901 3910 5416 2119 Size: 63 1719 9 1506 5110 Fragments arranged by size: 5110 1719 1506 63 9 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8407 XmaI C'CCGG_G Cuts at: 1870 5645 5732 1870 Size: 3775 87 4545 Fragments arranged by size: 4545 3775 87 XmnI GAAnn'nnTTC Cuts at: 2456 2537 4316 6536 2456 Size: 81 1779 2220 4327 Fragments arranged by size: 4327 2220 1779 81 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6215 276 Size: 53 83 186 936 4681 2468 Fragments arranged by size: 4681 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SacII SrfI SwaI