Restriction map for
pFN21AB5062
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanII BbeI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII CfoI Cfr10I CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MamI
MluI MroI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AcsI 1753 GAATTT [GA]^AATT[CT]
AflIII 1819 ACATGT A^C[GA][CT]GT
AocI 779 CCTCAGG CC^T[ACGT]AGG
ApoI 1753 GAATTT [GA]^AATT[CT]
AseI 892 ATTAAT AT^TAAT
AsnI 892 ATTAAT AT^TAAT
Asp700I 1356 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 329 GAGCAC G[AT]GC[AT]^C
AvaII 238 GGACC G^G[AT]CC
BalI 1574 TGGCCA TGG^CCA
BanI 1228 GGTGCC G^G[CT][GA]CC
BbrPI 1888 CACGTG CAC^GTG
BcnI 1058 CCGGG CC^[GC]GG
BfaI 581 CTAG C^TAG
BsaI 220 GGTCTC GGTCTC(1/5)
BsiHKAI 329 GAGCAC G[AT]GC[AT]^C
BsmI 1715 GCATTC GAATGC(1/-1)
BspDI 1855 ATCGAT AT^CGAT
BspLU11I 1819 ACATGT A^CATGT
BsrDI 1680 CATTGC GCAATG(2/0)
BstYI 291 AGATCC [GA]^GATC[CT]
Bsu36I 779 CCTCAGG CC^T[ACGT]AGG
ClaI 1855 ATCGAT AT^CGAT
DraI 937 TTTAAA TTT^AAA
DraII 237 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 1574 TGGCCA [CT]^GGCC[GA]
Eco81I 779 CCTCAGG CC^T[ACGT]AGG
EcoO109I 237 GGGACCC [GA]G^G[ACGT]CC[CT]
FauI 61 CCCGC CCCGC(4/6)
HapII 1058 CCGG C^CGG
HincII 1009 GTCAAC GT[CT]^[GA]AC
HindII 1009 GTCAAC GT[CT]^[GA]AC
HpaII 1058 CCGG C^CGG
MaeI 581 CTAG C^TAG
MfeI 1925 CAATTG C^AATTG
MflI 291 AGATCC [GA]^GATC[CT]
MluNI 1574 TGGCCA TGG^CCA
MscI 1574 TGGCCA TGG^CCA
MspI 1058 CCGG C^CGG
MunI 1925 CAATTG C^AATTG
NciI 1058 CCGGG CC^[GC]GG
NcoI 1565 CCATGG C^CATGG
NspI 1819 ACATGT [GA]CATG^[CT]
PflMI 1720 CCACGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1888 CACGTG CAC^GTG
PmlI 1888 CACGTG CAC^GTG
PpuMI 237 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 892 ATTAAT AT^TAAT
PstI 869 CTGCAG CTGCA^G
PvuII 836 CAGCTG CAG^CTG
ScaI 1706 AGTACT AGT^ACT
SexAI 216 ACCTGGT A^CC[AT]GGT
SmlI 651 CTCAAG C^T[CT][GA]AG
SpeI 580 ACTAGT A^CTAGT
Van91I 1720 CCACGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 291 AGATCC [GA]^GATC[CT]
XmnI 1356 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 63 CGCG CG^CG
335 CGCG
BciVI 823 GGATAC GTATCC(6/5)
1384 GGATAC
BmyI 329 GAGCAC G[AGT]GC[ACT]^C
393 GGGCAC
BpmI 308 CTGGAG CTGGAG(16/14)
560 CTGGAG
BsaAI 367 TACGTG [CT]AC^GT[GA]
1888 CACGTG
BseRI 1014 CTCCTC GAGGAG(10/8)
563 GAGGAG
BsmAI 1030 GAGAC GTCTC(1/5)
221 GTCTC
Bsp1286I 329 GAGCAC G[AGT]GC[ACT]^C
393 GGGCAC
BspMI 339 ACCTGC ACCTGC(4/8)
1585 GCAGGT
BstUI 63 CGCG CG^CG
335 CGCG
Cac8I 441 GCTTGC GC[ACGT]^[ACGT]GC
804 GCCTGC
Cfr13I 238 GGACC G^G[ACGT]CC
802 GGGCC
MaeII 368 ACGT A^CGT
1889 ACGT
MslI 906 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1440 CATGAGGATG
MspA1I 836 CAGCTG C[AC]G^C[GT]G
1380 CAGCGG
MvnI 63 CGCG CG^CG
335 CGCG
SapI 433 GCTCTTC GCTCTTC(1/4)
1359 GCTCTTC
Sau96I 238 GGACC G^G[ACGT]CC
802 GGGCC
Tsp45I 36 GTCAC ^GT[GC]AC
1201 GTCAC
XcmI 614 CCATCTTGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1269 CCAGAACAGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwI BbsI
BbvI BpuAI BsaJI BsiYI BslI
BsmFI BspHI BsrI BssKI BstF5I
BstNI BstXI DdeI DpnII DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI RcaI RsaI Sau3AI ScrFI
SfaNI SfcI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I