Restriction map for pFN21AB5062
 (Circular) (Six-base) MAPSORT of: pFN21AB5062.seq  Check: 9566  from: 1  to: 6670



pFN21AB5062  6670 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:33  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4480     278 
   Size:         53      83     186     936    2944    2468

  Fragments arranged by size: 

                2944    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    4013    1937 
   Size:       2076    4594
 
Acc65I G'GTAC_C

Cuts at:   3991    3991 
   Size:       6670
 
AclI AA'CG_TT

Cuts at:   1563    4797    5170    1563 
   Size:       3234     373    3063

  Fragments arranged by size: 

                3234    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2108    2121    2168    3692    3700    4733    6117    6385 
   Size:        476      13      47    1524       8    1033    1384     268

Cuts at:   6385    1632 
   Size:       1917

  Fragments arranged by size: 

                1917    1524    1384    1033     476     268      47      13
                   8
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6473
 
AflIII A'CryG_T

Cuts at:   1203    3824    5569    6546    1203 
   Size:       2621    1745     977    1327

  Fragments arranged by size: 

                2621    1745    1327     977
 
AgeI A'CCGG_T

Cuts at:   5501    5501 
   Size:       6670
 
AhdI GACnn_n'nnGTC

Cuts at:   5399    5399 
   Size:       6670
 
AleI CACnn'nnGTG

Cuts at:   1166    2915    1166 
   Size:       1749    4921
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6670
 
AlwNI CAG_nnn'CTG

Cuts at:   4413    5985    4413 
   Size:       1572    5098
 
ApaLI G'TGCA_C

Cuts at:   4727    5883    4727 
   Size:       1156    5514
 
ApoI r'AATT_y

Cuts at:   1493    3758    3979    4205    4357    6466    1493 
   Size:       2265     221     226     152    2109    1697

  Fragments arranged by size: 

                2265    2109    1697     226     221     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2898    5224     160 
   Size:        872    1866    2326    1606

  Fragments arranged by size: 

                2326    1866    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6670
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3995    1870 
   Size:         73    2052    4545

  Fragments arranged by size: 

                4545    2052      73
 
BamHI G'GATC_C

Cuts at:   4000    4367    4000 
   Size:        367    6303
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3233    3991    5446     618 
   Size:        325     217     627    1446     758    1455    1842

  Fragments arranged by size: 

                1842    1455    1446     758     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3989    1391 
   Size:       2598    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2001    2797    2943    3085     928 
   Size:        971     102     796     146     142    4513

  Fragments arranged by size: 

                4513     971     796     146     142     102
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4861    4895    1903 
   Size:         34    2924      34    3678

  Fragments arranged by size: 

                3678    2924      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2822    3383    4562    5772    2822 
   Size:        561    1179    1210    3720

  Fragments arranged by size: 

                3720    1210    1179     561
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6670
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5281     136 
   Size:        107     122      71     631     605    3609    1525

  Fragments arranged by size: 

                3609    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6665    6665 
   Size:       6670
 
BlpI GC'TnA_GC

Cuts at:   4087    4087 
   Size:       6670
 
Bme1580I G_kGCm'C

Cuts at:   1790    2402    4731    5887    1790 
   Size:        612    2329    1156    2573

  Fragments arranged by size: 

                2573    2329    1156     612
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6670
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2030    3227    4401 
   Size:        416      53      83     399      24     613    1197    1174

Cuts at:   4401    5359     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1197    1174     958     613     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6670
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2334    2586    5330    1239 
   Size:       1095     252    2744    2579

  Fragments arranged by size: 

                2744    2579    1095     252
 
Bpu10I CC'TnA_GC

Cuts at:   2814    2814 
   Size:       6670
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2641    4795    5660    5958    2641 
   Size:       2154     865     298    3353

  Fragments arranged by size: 

                3353    2154     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2231    5333     882 
   Size:        617     732    3102    2219

  Fragments arranged by size: 

                3102    2219     732     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2374    3895    4461     493 
   Size:        711    1170    1521     566    2702

  Fragments arranged by size: 

                2702    1521    1170     711     566
 
BsaBI GATnn'nnATC

Cuts at:   6664    6664 
   Size:       6670
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4477    4859    5494     275 
   Size:         53      83     186     936    2944     382     635    1451

  Fragments arranged by size: 

                2944    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   5103    5501    5775    5922    6333    5103 
   Size:        398     274     147     411    5440

  Fragments arranged by size: 

                5440     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3101    3131    1178 
   Size:         30     165      30    1698      30    4717

  Fragments arranged by size: 

                4717    1698     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2583    3010    1186 
   Size:       1397     427    4846

  Fragments arranged by size: 

                4846    1397     427
 
BseYI C'CCAG_C

Cuts at:   3169    3284    5873    6369    3169 
   Size:        115    2589     496    3470

  Fragments arranged by size: 

                3470    2589     496     115
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    6097
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4120    4881    5909    1540 
   Size:        275     186    2119     761    1028    2301

  Fragments arranged by size: 

                2301    2119    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2338    4731    4816    5887    2338 
   Size:       2393      85    1071    3121

  Fragments arranged by size: 

                3121    2393    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3720    4181    4274    3720 
   Size:        461      93    6116

  Fragments arranged by size: 

                6116     461      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2338    2402    3989    4731    4816    5887    1391 
   Size:        399     548      64    1587     742      85    1071    2174

  Fragments arranged by size: 

                2174    1587    1071     742     548     399      85      64
            
 
BspEI T'CCGG_A

Cuts at:   6333    6333 
   Size:       6670
 
BspHI T'CATG_A

Cuts at:   2214    2467    3624    4559    6289    2214 
   Size:        253    1157     935    1730    2595

  Fragments arranged by size: 

                2595    1730    1157     935     253
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2353    3581    4025     844 
   Size:       1509    1228     444    3489

  Fragments arranged by size: 

                3489    1509    1228     444
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4557    1394 
   Size:        429    2734    3507

  Fragments arranged by size: 

                3507    2734     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3684    5165    5339      65 
   Size:       1183    2436    1481     174    1396

  Fragments arranged by size: 

                2436    1481    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5314    5501    1700 
   Size:        110    3504     187    2869

  Fragments arranged by size: 

                3504    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6670
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6670
 
BssSI C'ACGA_G

Cuts at:   4730    5742    4730 
   Size:       1012    5658
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6670
 
BstBI TT'CG_AA

Cuts at:   5474    5474 
   Size:       6670
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6670
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2917    3571    3577    2917 
   Size:        654       6    6010

  Fragments arranged by size: 

                6010     654       6
 
BstYI r'GATC_y

Cuts at:   1972    2296    4000    4367    4752    4769    6221    6307    6319 
   Size:        324    1704     367     385      17    1452      86      12

Cuts at:   6319    6665    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1704    1452     385     367     346     324      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2785    2785 
   Size:       6670
 
BtgI C'CryG_G

Cuts at:    513    1065    2554    2803    3336    3570     513 
   Size:        552    1489     249     533     234    3613

  Fragments arranged by size: 

                3613    1489     552     533     249     234
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5931

  Fragments arranged by size: 

                5931     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2910    3331    4186    4980    5000    2910 
   Size:        421     855     794      20    4580

  Fragments arranged by size: 

                4580     855     794     421      20
 
ClaI AT'CG_AT

Cuts at:   1554    3861    1554 
   Size:       2307    4363
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6635
 
DraI TTT'AAA

Cuts at:   2944    3974    2944 
   Size:       1030    5640
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5677     809 
   Size:        507    4361    1802

  Fragments arranged by size: 

                4361    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3579    4117    5006 
   Size:         54    1425      50     275      18    1749     538     889

Cuts at:   5006    6635       8 
   Size:       1629      43

  Fragments arranged by size: 

                1749    1629    1425     889     538     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4117    1537 
   Size:        275    2305    4090

  Fragments arranged by size: 

                4090    2305     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2144    2445    3299    3371    4600    1353 
   Size:        791     301     854      72    1229    3423

  Fragments arranged by size: 

                3423    1229     854     791     301      72
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5257    5631    5777     387 
   Size:       1064     138    3668     374     146    1280

  Fragments arranged by size: 

                3668    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2108    2121    2168    2334    2586    3692    3700 
   Size:        393     476      13      47     166     252    1106       8

Cuts at:   3700    4733    5330    6117    6385    1239 
   Size:       1033     597     787     268    1524

  Fragments arranged by size: 

                1524    1106    1033     787     597     476     393     268
                 252     166      47      13       8
 
EcoO109I rG'GnC_Cy

Cuts at:   2243    2243 
   Size:       6670
 
EcoRI G'AATT_C

Cuts at:   3979    4357    3979 
   Size:        378    6292
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2173    3382    2173 
   Size:       1209    5461
 
FspI TGC'GCA

Cuts at:   5176    5176 
   Size:       6670
 
HaeII r_GCGC'y

Cuts at:   1678    5817    1678 
   Size:       4139    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2386    2418    2700    2732    2733 
   Size:         32     135      32     814      32     282      32       1

Cuts at:   2733    2765    5311    5343    5385    5417    1373 
   Size:         32    2546      32      42      32    2626

  Fragments arranged by size: 

                2626    2546     814     282     135      42      32      32
                  32      32      32      32      32       1
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3016    4014    4260     669 
   Size:        988     281    1078     998     246    3079

  Fragments arranged by size: 

                3079    1078     998     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6670
 
HpaI GTT'AAC

Cuts at:   4260    4260 
   Size:       6670
 
KpnI G_GTAC'C

Cuts at:   3995    3995 
   Size:       6670
 
MfeI C'AATT_G

Cuts at:   3930    4269    3930 
   Size:        339    6331
 
MluI A'CGCG_T

Cuts at:   6546    6546 
   Size:       6670
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2518    5784    5968    1305 
   Size:       1213    3266     184    2007

  Fragments arranged by size: 

                3266    2007    1213     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    3581      10 
   Size:         54    1425     343    1749    3099

  Fragments arranged by size: 

                3099    1749    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2915    3449    4628    4987    5146     518 
   Size:        648    1749     534    1179     359     159    2042

  Fragments arranged by size: 

                2042    1749    1179     648     534     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2843    3387    4086    4763    5911    6156    1606 
   Size:       1237     544     699     677    1148     245    2120

  Fragments arranged by size: 

                2120    1237    1148     699     677     544     245
 
NcoI C'CATG_G

Cuts at:    513    1065    3570     513 
   Size:        552    2505    3613

  Fragments arranged by size: 

                3613    2505     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6670
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6670
 
NotI GC'GGCC_GC

Cuts at:   4117    4117 
   Size:       6670
 
NspI r_CATG'y

Cuts at:   1132    1229    3828    4028    5573    1132 
   Size:         97    2599     200    1545    2229

  Fragments arranged by size: 

                2599    2229    1545     200      97
 
PasI CC'CwG_GG

Cuts at:   1348    3873    1348 
   Size:       2525    4145
 
PciI A'CATG_T

Cuts at:   3824    5569    3824 
   Size:       1745    4925
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3731    1108 
   Size:       2623    4047
 
PmeI GTTT'AAAC

Cuts at:   3974    3974 
   Size:       6670
 
PmlI CAC'GTG

Cuts at:   3895    3895 
   Size:       6670
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3273    3305    4739    4771    6252    6284    3273 
   Size:         32    1434      32    1481      32    3659

  Fragments arranged by size: 

                3659    1481    1434      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2243    2243 
   Size:       6670
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6670
 
PsiI TTA'TAA

Cuts at:   4240    4240 
   Size:       6670
 
PstI C_TGCA'G

Cuts at:    830    2878    4022     830 
   Size:       2048    1144    3478

  Fragments arranged by size: 

                3478    2048    1144
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6670
 
PvuII CAG'CTG

Cuts at:   2843    2843 
   Size:       6670
 
SalI G'TCGA_C

Cuts at:   1936    4012    1936 
   Size:       2076    4594
 
SanDI GG'GwC_CC

Cuts at:   2243    2243 
   Size:       6670
 
SapI GCTCTTCn'nnn_

Cuts at:   2445    3371    2445 
   Size:        926    5744
 
SbfI CC_TGCA'GG

Cuts at:   4022    4022 
   Size:       6670
 
ScaI AGT'ACT

Cuts at:   3713    4918    6630    3713 
   Size:       1205    1712    3753

  Fragments arranged by size: 

                3753    1712    1205
 
SexAI A'CCwGG_T

Cuts at:   2221    2221 
   Size:       6670
 
SfcI C'TryA_G

Cuts at:    826    1045    2874    3189    3394    3609    4018    5153    5834 
   Size:        219    1829     315     205     215     409    1135     681

Cuts at:   5834    6025     826 
   Size:        191    1471

  Fragments arranged by size: 

                1829    1471    1135     681     409     315     219     215
                 205     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6670
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6670
 
SmaI CCC'GGG

Cuts at:   1872    3997    1872 
   Size:       2125    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2656    4774    5675    5937     820 
   Size:        197     926     713    2118     901     262    1553

  Fragments arranged by size: 

                2118    1553     926     901     713     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4461     493 
   Size:       3968    2702
 
SpeI A'CTAG_T

Cuts at:    152    2585     152 
   Size:       2433    4237
 
SphI G_CATG'C

Cuts at:   1132    4028    1132 
   Size:       2896    3774
 
SspI AAT'ATT

Cuts at:      5      52    2193    3842    3965    4594       5 
   Size:         47    2141    1649     123     629    2081

  Fragments arranged by size: 

                2141    2081    1649     629     123      47
 
StuI AGG'CCT

Cuts at:   1517    2822    2861    3185    1517 
   Size:       1305      39     324    5002

  Fragments arranged by size: 

                5002    1305     324      39
 
StyI C'CwwG_G

Cuts at:    513    1065    2532    2823    3415    3570    3900    4109     513 
   Size:        552    1467     291     592     155     330     209    3074

  Fragments arranged by size: 

                3074    1467     592     552     330     291     209     155
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4697    4714    4867    5052    5497     325 
   Size:       1255    3117      17     153     185     445    1498

  Fragments arranged by size: 

                3117    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3483    3711    3827 
   Size:        274      80      33      51    1443    1506     228     116

Cuts at:   3827    4916    6628      96 
   Size:       1089    1712     138

  Fragments arranged by size: 

                1712    1506    1443    1089     274     228     138     116
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6630    6662    6630 
   Size:         32    6638
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6670
 
XbaI T'CTAG_A

Cuts at:   4006    4006 
   Size:       6670
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2626    3281    2626 
   Size:        655    6015
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6670
 
XmaI C'CCGG_G

Cuts at:   1870    3995    1870 
   Size:       2125    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3365    4799    3365 
   Size:       1434    5236
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4478     276 
   Size:         53      83     186     936    2944    2468

  Fragments arranged by size: 

                2944    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
     PstI     PvuI    PvuII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BsmBI  BstZ17I   DraIII  EcoICRI
    EcoNI    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI   PspOMI    PspXI     PsrI    RsrII
     SacI    SacII     SfiI     SfoI     SrfI     SwaI
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