(Circular) (Six-base) MAPSORT of: pFN21AB5062.seq Check: 9566 from: 1 to: 6670 pFN21AB5062 6670 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4480 278 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4013 1937 Size: 2076 4594 Acc65I G'GTAC_C Cuts at: 3991 3991 Size: 6670 AclI AA'CG_TT Cuts at: 1563 4797 5170 1563 Size: 3234 373 3063 Fragments arranged by size: 3234 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2108 2121 2168 3692 3700 4733 6117 6385 Size: 476 13 47 1524 8 1033 1384 268 Cuts at: 6385 1632 Size: 1917 Fragments arranged by size: 1917 1524 1384 1033 476 268 47 13 8 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6473 AflIII A'CryG_T Cuts at: 1203 3824 5569 6546 1203 Size: 2621 1745 977 1327 Fragments arranged by size: 2621 1745 1327 977 AgeI A'CCGG_T Cuts at: 5501 5501 Size: 6670 AhdI GACnn_n'nnGTC Cuts at: 5399 5399 Size: 6670 AleI CACnn'nnGTG Cuts at: 1166 2915 1166 Size: 1749 4921 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6670 AlwNI CAG_nnn'CTG Cuts at: 4413 5985 4413 Size: 1572 5098 ApaLI G'TGCA_C Cuts at: 4727 5883 4727 Size: 1156 5514 ApoI r'AATT_y Cuts at: 1493 3758 3979 4205 4357 6466 1493 Size: 2265 221 226 152 2109 1697 Fragments arranged by size: 2265 2109 1697 226 221 152 AseI AT'TA_AT Cuts at: 160 1032 2898 5224 160 Size: 872 1866 2326 1606 Fragments arranged by size: 2326 1866 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 AvaI C'yCGr_G Cuts at: 1870 1943 3995 1870 Size: 73 2052 4545 Fragments arranged by size: 4545 2052 73 BamHI G'GATC_C Cuts at: 4000 4367 4000 Size: 367 6303 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3233 3991 5446 618 Size: 325 217 627 1446 758 1455 1842 Fragments arranged by size: 1842 1455 1446 758 627 325 217 BanII G_rGCy'C Cuts at: 1391 3989 1391 Size: 2598 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2001 2797 2943 3085 928 Size: 971 102 796 146 142 4513 Fragments arranged by size: 4513 971 796 146 142 102 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4861 4895 1903 Size: 34 2924 34 3678 Fragments arranged by size: 3678 2924 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2822 3383 4562 5772 2822 Size: 561 1179 1210 3720 Fragments arranged by size: 3720 1210 1179 561 BclI T'GATC_A Cuts at: 1548 1548 Size: 6670 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5281 136 Size: 107 122 71 631 605 3609 1525 Fragments arranged by size: 3609 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6665 6665 Size: 6670 BlpI GC'TnA_GC Cuts at: 4087 4087 Size: 6670 Bme1580I G_kGCm'C Cuts at: 1790 2402 4731 5887 1790 Size: 612 2329 1156 2573 Fragments arranged by size: 2573 2329 1156 612 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6670 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2030 3227 4401 Size: 416 53 83 399 24 613 1197 1174 Cuts at: 4401 5359 442 Size: 958 1753 Fragments arranged by size: 1753 1197 1174 958 613 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6670 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2334 2586 5330 1239 Size: 1095 252 2744 2579 Fragments arranged by size: 2744 2579 1095 252 Bpu10I CC'TnA_GC Cuts at: 2814 2814 Size: 6670 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2641 4795 5660 5958 2641 Size: 2154 865 298 3353 Fragments arranged by size: 3353 2154 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2231 5333 882 Size: 617 732 3102 2219 Fragments arranged by size: 3102 2219 732 617 BsaAI yAC'GTr Cuts at: 493 1204 2374 3895 4461 493 Size: 711 1170 1521 566 2702 Fragments arranged by size: 2702 1521 1170 711 566 BsaBI GATnn'nnATC Cuts at: 6664 6664 Size: 6670 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4477 4859 5494 275 Size: 53 83 186 936 2944 382 635 1451 Fragments arranged by size: 2944 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5103 5501 5775 5922 6333 5103 Size: 398 274 147 411 5440 Fragments arranged by size: 5440 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3101 3131 1178 Size: 30 165 30 1698 30 4717 Fragments arranged by size: 4717 1698 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2583 3010 1186 Size: 1397 427 4846 Fragments arranged by size: 4846 1397 427 BseYI C'CCAG_C Cuts at: 3169 3284 5873 6369 3169 Size: 115 2589 496 3470 Fragments arranged by size: 3470 2589 496 115 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6097 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4120 4881 5909 1540 Size: 275 186 2119 761 1028 2301 Fragments arranged by size: 2301 2119 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2338 4731 4816 5887 2338 Size: 2393 85 1071 3121 Fragments arranged by size: 3121 2393 1071 85 BsmI GAATG_Cn' Cuts at: 3720 4181 4274 3720 Size: 461 93 6116 Fragments arranged by size: 6116 461 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2338 2402 3989 4731 4816 5887 1391 Size: 399 548 64 1587 742 85 1071 2174 Fragments arranged by size: 2174 1587 1071 742 548 399 85 64 BspEI T'CCGG_A Cuts at: 6333 6333 Size: 6670 BspHI T'CATG_A Cuts at: 2214 2467 3624 4559 6289 2214 Size: 253 1157 935 1730 2595 Fragments arranged by size: 2595 1730 1157 935 253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2353 3581 4025 844 Size: 1509 1228 444 3489 Fragments arranged by size: 3489 1509 1228 444 BsrBI CCG'CTC Cuts at: 1394 1823 4557 1394 Size: 429 2734 3507 Fragments arranged by size: 3507 2734 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3684 5165 5339 65 Size: 1183 2436 1481 174 1396 Fragments arranged by size: 2436 1481 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5314 5501 1700 Size: 110 3504 187 2869 Fragments arranged by size: 3504 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6670 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6670 BssSI C'ACGA_G Cuts at: 4730 5742 4730 Size: 1012 5658 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6670 BstBI TT'CG_AA Cuts at: 5474 5474 Size: 6670 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6670 BstXI CCAn_nnnn'nTGG Cuts at: 2917 3571 3577 2917 Size: 654 6 6010 Fragments arranged by size: 6010 654 6 BstYI r'GATC_y Cuts at: 1972 2296 4000 4367 4752 4769 6221 6307 6319 Size: 324 1704 367 385 17 1452 86 12 Cuts at: 6319 6665 1972 Size: 346 1977 Fragments arranged by size: 1977 1704 1452 385 367 346 324 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2785 2785 Size: 6670 BtgI C'CryG_G Cuts at: 513 1065 2554 2803 3336 3570 513 Size: 552 1489 249 533 234 3613 Fragments arranged by size: 3613 1489 552 533 249 234 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5931 Fragments arranged by size: 5931 683 56 BtsI GCAGTG_nn' Cuts at: 2910 3331 4186 4980 5000 2910 Size: 421 855 794 20 4580 Fragments arranged by size: 4580 855 794 421 20 ClaI AT'CG_AT Cuts at: 1554 3861 1554 Size: 2307 4363 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6635 DraI TTT'AAA Cuts at: 2944 3974 2944 Size: 1030 5640 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5677 809 Size: 507 4361 1802 Fragments arranged by size: 4361 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3579 4117 5006 Size: 54 1425 50 275 18 1749 538 889 Cuts at: 5006 6635 8 Size: 1629 43 Fragments arranged by size: 1749 1629 1425 889 538 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4117 1537 Size: 275 2305 4090 Fragments arranged by size: 4090 2305 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2144 2445 3299 3371 4600 1353 Size: 791 301 854 72 1229 3423 Fragments arranged by size: 3423 1229 854 791 301 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5257 5631 5777 387 Size: 1064 138 3668 374 146 1280 Fragments arranged by size: 3668 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2121 2168 2334 2586 3692 3700 Size: 393 476 13 47 166 252 1106 8 Cuts at: 3700 4733 5330 6117 6385 1239 Size: 1033 597 787 268 1524 Fragments arranged by size: 1524 1106 1033 787 597 476 393 268 252 166 47 13 8 EcoO109I rG'GnC_Cy Cuts at: 2243 2243 Size: 6670 EcoRI G'AATT_C Cuts at: 3979 4357 3979 Size: 378 6292 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2173 3382 2173 Size: 1209 5461 FspI TGC'GCA Cuts at: 5176 5176 Size: 6670 HaeII r_GCGC'y Cuts at: 1678 5817 1678 Size: 4139 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2386 2418 2700 2732 2733 Size: 32 135 32 814 32 282 32 1 Cuts at: 2733 2765 5311 5343 5385 5417 1373 Size: 32 2546 32 42 32 2626 Fragments arranged by size: 2626 2546 814 282 135 42 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 3016 4014 4260 669 Size: 988 281 1078 998 246 3079 Fragments arranged by size: 3079 1078 998 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6670 HpaI GTT'AAC Cuts at: 4260 4260 Size: 6670 KpnI G_GTAC'C Cuts at: 3995 3995 Size: 6670 MfeI C'AATT_G Cuts at: 3930 4269 3930 Size: 339 6331 MluI A'CGCG_T Cuts at: 6546 6546 Size: 6670 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2518 5784 5968 1305 Size: 1213 3266 184 2007 Fragments arranged by size: 3266 2007 1213 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3581 10 Size: 54 1425 343 1749 3099 Fragments arranged by size: 3099 1749 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2915 3449 4628 4987 5146 518 Size: 648 1749 534 1179 359 159 2042 Fragments arranged by size: 2042 1749 1179 648 534 359 159 MspA1I CmG'CkG Cuts at: 1606 2843 3387 4086 4763 5911 6156 1606 Size: 1237 544 699 677 1148 245 2120 Fragments arranged by size: 2120 1237 1148 699 677 544 245 NcoI C'CATG_G Cuts at: 513 1065 3570 513 Size: 552 2505 3613 Fragments arranged by size: 3613 2505 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6670 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6670 NotI GC'GGCC_GC Cuts at: 4117 4117 Size: 6670 NspI r_CATG'y Cuts at: 1132 1229 3828 4028 5573 1132 Size: 97 2599 200 1545 2229 Fragments arranged by size: 2599 2229 1545 200 97 PasI CC'CwG_GG Cuts at: 1348 3873 1348 Size: 2525 4145 PciI A'CATG_T Cuts at: 3824 5569 3824 Size: 1745 4925 PflMI CCAn_nnn'nTGG Cuts at: 1108 3731 1108 Size: 2623 4047 PmeI GTTT'AAAC Cuts at: 3974 3974 Size: 6670 PmlI CAC'GTG Cuts at: 3895 3895 Size: 6670 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3273 3305 4739 4771 6252 6284 3273 Size: 32 1434 32 1481 32 3659 Fragments arranged by size: 3659 1481 1434 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2243 2243 Size: 6670 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6670 PsiI TTA'TAA Cuts at: 4240 4240 Size: 6670 PstI C_TGCA'G Cuts at: 830 2878 4022 830 Size: 2048 1144 3478 Fragments arranged by size: 3478 2048 1144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6670 PvuII CAG'CTG Cuts at: 2843 2843 Size: 6670 SalI G'TCGA_C Cuts at: 1936 4012 1936 Size: 2076 4594 SanDI GG'GwC_CC Cuts at: 2243 2243 Size: 6670 SapI GCTCTTCn'nnn_ Cuts at: 2445 3371 2445 Size: 926 5744 SbfI CC_TGCA'GG Cuts at: 4022 4022 Size: 6670 ScaI AGT'ACT Cuts at: 3713 4918 6630 3713 Size: 1205 1712 3753 Fragments arranged by size: 3753 1712 1205 SexAI A'CCwGG_T Cuts at: 2221 2221 Size: 6670 SfcI C'TryA_G Cuts at: 826 1045 2874 3189 3394 3609 4018 5153 5834 Size: 219 1829 315 205 215 409 1135 681 Cuts at: 5834 6025 826 Size: 191 1471 Fragments arranged by size: 1829 1471 1135 681 409 315 219 215 205 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6670 SmaI CCC'GGG Cuts at: 1872 3997 1872 Size: 2125 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2656 4774 5675 5937 820 Size: 197 926 713 2118 901 262 1553 Fragments arranged by size: 2118 1553 926 901 713 262 197 SnaBI TAC'GTA Cuts at: 493 4461 493 Size: 3968 2702 SpeI A'CTAG_T Cuts at: 152 2585 152 Size: 2433 4237 SphI G_CATG'C Cuts at: 1132 4028 1132 Size: 2896 3774 SspI AAT'ATT Cuts at: 5 52 2193 3842 3965 4594 5 Size: 47 2141 1649 123 629 2081 Fragments arranged by size: 2141 2081 1649 629 123 47 StuI AGG'CCT Cuts at: 1517 2822 2861 3185 1517 Size: 1305 39 324 5002 Fragments arranged by size: 5002 1305 324 39 StyI C'CwwG_G Cuts at: 513 1065 2532 2823 3415 3570 3900 4109 513 Size: 552 1467 291 592 155 330 209 3074 Fragments arranged by size: 3074 1467 592 552 330 291 209 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4697 4714 4867 5052 5497 325 Size: 1255 3117 17 153 185 445 1498 Fragments arranged by size: 3117 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3483 3711 3827 Size: 274 80 33 51 1443 1506 228 116 Cuts at: 3827 4916 6628 96 Size: 1089 1712 138 Fragments arranged by size: 1712 1506 1443 1089 274 228 138 116 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6630 6662 6630 Size: 32 6638 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6670 XbaI T'CTAG_A Cuts at: 4006 4006 Size: 6670 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2626 3281 2626 Size: 655 6015 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6670 XmaI C'CCGG_G Cuts at: 1870 3995 1870 Size: 2125 4545 XmnI GAAnn'nnTTC Cuts at: 3365 4799 3365 Size: 1434 5236 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4478 276 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI