Restriction map for
pFN21AB5062
(Linear) (Six-base) MAPSORT of: KIBB5062.seq Check: 1020 from: 1 to: 1965
KIBB5062.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 116 163 1687 1695 1965
Size: 103 13 47 1524 8 270
Fragments arranged by size:
1524 270 103 47 13 8
AflIII A'CryG_T
Cuts at: 0 1819 1965
Size: 1819 146
AleI CACnn'nnGTG
Cuts at: 0 910 1965
Size: 910 1055
ApoI r'AATT_y
Cuts at: 0 1753 1965
Size: 1753 212
AseI AT'TA_AT
Cuts at: 0 893 1965
Size: 893 1072
BanI G'GyrC_C
Cuts at: 0 1228 1965
Size: 1228 737
BbsI GAAGACnn'nnnn_
Cuts at: 0 792 938 1080 1965
Size: 792 146 142 885
Fragments arranged by size:
885 792 146 142
BciVI GTATCCnnnnn_n'
Cuts at: 0 817 1378 1965
Size: 817 561 587
Fragments arranged by size:
817 587 561
Bme1580I G_kGCm'C
Cuts at: 0 397 1965
Size: 397 1568
BmrI ACTGGGnnnn_n'
Cuts at: 0 25 1222 1965
Size: 25 1197 743
Fragments arranged by size:
1197 743 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 581 1965
Size: 329 252 1384
Fragments arranged by size:
1384 329 252
Bpu10I CC'TnA_GC
Cuts at: 0 809 1965
Size: 809 1156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 1965
Size: 636 1329
BsaI GGTCTCn'nnnn_
Cuts at: 0 226 1965
Size: 226 1739
BsaAI yAC'GTr
Cuts at: 0 369 1890 1965
Size: 369 1521 75
Fragments arranged by size:
1521 369 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1096 1126 1965
Size: 1096 30 839
Fragments arranged by size:
1096 839 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 578 1005 1965
Size: 578 427 960
Fragments arranged by size:
960 578 427
BseYI C'CCAG_C
Cuts at: 0 1164 1279 1965
Size: 1164 115 686
Fragments arranged by size:
1164 686 115
BsiHKAI G_wGCw'C
Cuts at: 0 333 1965
Size: 333 1632
BsmI GAATG_Cn'
Cuts at: 0 1715 1965
Size: 1715 250
Bsp1286I G_dGCh'C
Cuts at: 0 333 397 1965
Size: 333 64 1568
Fragments arranged by size:
1568 333 64
BspHI T'CATG_A
Cuts at: 0 209 462 1619 1965
Size: 209 253 1157 346
Fragments arranged by size:
1157 346 253 209
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 348 1576 1965
Size: 348 1228 389
Fragments arranged by size:
1228 389 348
BsrDI GCAATG_nn'
Cuts at: 0 1679 1965
Size: 1679 286
BstXI CCAn_nnnn'nTGG
Cuts at: 0 912 1566 1572 1965
Size: 912 654 6 393
Fragments arranged by size:
912 654 393 6
BstYI r'GATC_y
Cuts at: 0 291 1965
Size: 291 1674
Bsu36I CC'TnA_GG
Cuts at: 0 780 1965
Size: 780 1185
BtgI C'CryG_G
Cuts at: 0 549 798 1331 1565 1965
Size: 549 249 533 234 400
Fragments arranged by size:
549 533 400 249 234
BtsI GCAGTG_nn'
Cuts at: 0 905 1326 1965
Size: 905 421 639
Fragments arranged by size:
905 639 421
ClaI AT'CG_AT
Cuts at: 0 1856 1965
Size: 1856 109
DraI TTT'AAA
Cuts at: 0 939 1965
Size: 939 1026
EaeI y'GGCC_r
Cuts at: 0 1574 1965
Size: 1574 391
EarI CTCTTCn'nnn_
Cuts at: 0 139 440 1294 1366 1965
Size: 139 301 854 72 599
Fragments arranged by size:
854 599 301 139 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 116 163 329 581 1687 1695 1965
Size: 103 13 47 166 252 1106 8 270
Fragments arranged by size:
1106 270 252 166 103 47 13 8
EcoO109I rG'GnC_Cy
Cuts at: 0 238 1965
Size: 238 1727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 168 1377 1965
Size: 168 1209 588
Fragments arranged by size:
1209 588 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 381 413 695 727 728 760 1965
Size: 381 32 282 32 1 32 1205
Fragments arranged by size:
1205 381 282 32 32 32 1
HincII GTy'rAC
Cuts at: 0 1011 1965
Size: 1011 954
MfeI C'AATT_G
Cuts at: 0 1925 1965
Size: 1925 40
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 1965
Size: 513 1452
MscI TGG'CCA
Cuts at: 0 1576 1965
Size: 1576 389
MslI CAynn'nnrTG
Cuts at: 0 910 1444 1965
Size: 910 534 521
Fragments arranged by size:
910 534 521
MspA1I CmG'CkG
Cuts at: 0 838 1382 1965
Size: 838 544 583
Fragments arranged by size:
838 583 544
NcoI C'CATG_G
Cuts at: 0 1565 1965
Size: 1565 400
NspI r_CATG'y
Cuts at: 0 1823 1965
Size: 1823 142
PasI CC'CwG_GG
Cuts at: 0 1868 1965
Size: 1868 97
PciI A'CATG_T
Cuts at: 0 1819 1965
Size: 1819 146
PflMI CCAn_nnn'nTGG
Cuts at: 0 1726 1965
Size: 1726 239
PmlI CAC'GTG
Cuts at: 0 1890 1965
Size: 1890 75
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1268 1300 1965
Size: 1268 32 665
Fragments arranged by size:
1268 665 32
PpuMI rG'GwC_Cy
Cuts at: 0 238 1965
Size: 238 1727
PstI C_TGCA'G
Cuts at: 0 873 1965
Size: 873 1092
PvuII CAG'CTG
Cuts at: 0 838 1965
Size: 838 1127
SanDI GG'GwC_CC
Cuts at: 0 238 1965
Size: 238 1727
SapI GCTCTTCn'nnn_
Cuts at: 0 440 1366 1965
Size: 440 926 599
Fragments arranged by size:
926 599 440
ScaI AGT'ACT
Cuts at: 0 1708 1965
Size: 1708 257
SexAI A'CCwGG_T
Cuts at: 0 216 1965
Size: 216 1749
SfcI C'TryA_G
Cuts at: 0 869 1184 1389 1604 1965
Size: 869 315 205 215 361
Fragments arranged by size:
869 361 315 215 205
SmlI C'TyrA_G
Cuts at: 0 651 1965
Size: 651 1314
SpeI A'CTAG_T
Cuts at: 0 580 1965
Size: 580 1385
SspI AAT'ATT
Cuts at: 0 188 1837 1960 1965
Size: 188 1649 123 5
Fragments arranged by size:
1649 188 123 5
StuI AGG'CCT
Cuts at: 0 817 856 1180 1965
Size: 817 39 324 785
Fragments arranged by size:
817 785 324 39
StyI C'CwwG_G
Cuts at: 0 527 818 1410 1565 1895 1965
Size: 527 291 592 155 330 70
Fragments arranged by size:
592 527 330 291 155 70
TatI w'GTAC_w
Cuts at: 0 1478 1706 1822 1965
Size: 1478 228 116 143
Fragments arranged by size:
1478 228 143 116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 621 1276 1965
Size: 621 655 689
Fragments arranged by size:
689 655 621
XmnI GAAnn'nnTTC
Cuts at: 0 1360 1965
Size: 1360 605
Enzymes that do cut:
AcuI AflIII AleI ApoI AseI BanI BbsI BciVI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BstXI BstYI Bsu36I BtgI BtsI ClaI DraI EaeI
EarI Eco57MI EcoO109I FalI Hin4I HincII MfeI MmeI
MscI MslI MspA1I NcoI NspI PasI PciI PflMI
PmlI PpiI PpuMI PstI PvuII SanDI SapI ScaI
SexAI SfcI SmlI SpeI SspI StuI StyI TatI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PfoI PmeI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SwaI TaqII TstI Tth111I XbaI XhoI
XmaI ZraI