Restriction map for
pFN21AB5053
(Linear) (Six-base) MAPSORT of: KIBB5053.seq Check: 3822 from: 1 to: 2340
KIBB5053.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 460 2340
Size: 460 1880
AccI GT'mk_AC
Cuts at: 0 815 2340
Size: 815 1525
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 766 1054 2116 2206 2233 2340
Size: 691 75 288 1062 90 27 107
Fragments arranged by size:
1062 691 288 107 90 75 27
AleI CACnn'nnGTG
Cuts at: 0 883 2340
Size: 883 1457
AlwNI CAG_nnn'CTG
Cuts at: 0 1192 1209 1692 1701 1798 2109 2184 2340
Size: 1192 17 483 9 97 311 75 156
Fragments arranged by size:
1192 483 311 156 97 75 17 9
ApaI G_GGCC'C
Cuts at: 0 1055 2340
Size: 1055 1285
ApoI r'AATT_y
Cuts at: 0 961 2340
Size: 961 1379
AvaI C'yCGr_G
Cuts at: 0 1450 2004 2340
Size: 1450 554 336
Fragments arranged by size:
1450 554 336
BamHI G'GATC_C
Cuts at: 0 656 2078 2340
Size: 656 1422 262
Fragments arranged by size:
1422 656 262
BanI G'GyrC_C
Cuts at: 0 3 472 628 978 1858 2340
Size: 3 469 156 350 880 482
Fragments arranged by size:
880 482 469 350 156 3
BanII G_rGCy'C
Cuts at: 0 116 709 1055 1678 2005 2340
Size: 116 593 346 623 327 335
Fragments arranged by size:
623 593 346 335 327 116
BbeI G_GCGC'C
Cuts at: 0 7 2340
Size: 7 2333
BbsI GAAGACnn'nnnn_
Cuts at: 0 944 2340
Size: 944 1396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1441 1475 2340
Size: 1441 34 865
Fragments arranged by size:
1441 865 34
BglI GCCn_nnn'nGGC
Cuts at: 0 94 313 605 1204 2340
Size: 94 219 292 599 1136
Fragments arranged by size:
1136 599 292 219 94
BglII A'GATC_T
Cuts at: 0 1301 2340
Size: 1301 1039
BlpI GC'TnA_GC
Cuts at: 0 1185 2340
Size: 1185 1155
Bme1580I G_kGCm'C
Cuts at: 0 679 1055 1549 1826 2313 2340
Size: 679 376 494 277 487 27
Fragments arranged by size:
679 494 487 376 277 27
BmgBI CAC'GTC
Cuts at: 0 1472 1671 2340
Size: 1472 199 669
Fragments arranged by size:
1472 669 199
BmrI ACTGGGnnnn_n'
Cuts at: 0 1230 2340
Size: 1230 1110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1235 1591 1723 1756 2047 2340
Size: 307 928 356 132 33 291 293
Fragments arranged by size:
928 356 307 293 291 132 33
Bpu10I CC'TnA_GC
Cuts at: 0 919 2340
Size: 919 1421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1207 1326 1498 1795 2340
Size: 1207 119 172 297 545
Fragments arranged by size:
1207 545 297 172 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 1447 1522 2080 2242 2340
Size: 1447 75 558 162 98
Fragments arranged by size:
1447 558 162 98 75
BsaBI GATnn'nnATC
Cuts at: 0 1753 2340
Size: 1753 587
BsaHI Gr'CG_yC
Cuts at: 0 4 2340
Size: 4 2336
BsaWI w'CCGG_w
Cuts at: 0 715 1578 2340
Size: 715 863 762
Fragments arranged by size:
863 762 715
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 658 688 808 838 1233 1263 2340
Size: 658 30 120 30 395 30 1077
Fragments arranged by size:
1077 658 395 120 30 30 30
BseYI C'CCAG_C
Cuts at: 0 652 2340
Size: 652 1688
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 881 1137 2340
Size: 813 68 256 1203
Fragments arranged by size:
1203 813 256 68
BsiEI CG_ry'CG
Cuts at: 0 184 277 933 2340
Size: 184 93 656 1407
Fragments arranged by size:
1407 656 184 93
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1976 2272 2340
Size: 1976 296 68
Fragments arranged by size:
1976 296 68
Bsp1286I G_dGCh'C
Cuts at: 0 116 679 709 1055 1549 1678 1826 2005
Size: 116 563 30 346 494 129 148 179
Cuts at: 2005 2313 2340
Size: 308 27
Fragments arranged by size:
563 494 346 308 179 148 129 116
30 27
BspEI T'CCGG_A
Cuts at: 0 1578 2340
Size: 1578 762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 146 272 425 460 1751 1856 2340
Size: 146 126 153 35 1291 105 484
Fragments arranged by size:
1291 484 153 146 126 105 35
BsrBI CCG'CTC
Cuts at: 0 1284 2340
Size: 1284 1056
BsrDI GCAATG_nn'
Cuts at: 0 941 1499 1905 2340
Size: 941 558 406 435
Fragments arranged by size:
941 558 435 406
BsrFI r'CCGG_y
Cuts at: 0 179 928 2340
Size: 179 749 1412
Fragments arranged by size:
1412 749 179
BssSI C'ACGA_G
Cuts at: 0 2083 2340
Size: 2083 257
BstXI CCAn_nnnn'nTGG
Cuts at: 0 808 2340
Size: 808 1532
BstYI r'GATC_y
Cuts at: 0 505 656 1265 1301 1311 1748 2078 2340
Size: 505 151 609 36 10 437 330 262
Fragments arranged by size:
609 505 437 330 262 151 36 10
BtgI C'CryG_G
Cuts at: 0 801 1215 1818 2340
Size: 801 414 603 522
Fragments arranged by size:
801 603 522 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 112 2340
Size: 112 2228
BtsI GCAGTG_nn'
Cuts at: 0 1934 2313 2340
Size: 1934 379 27
Fragments arranged by size:
1934 379 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1323 1358 1800 1835 2340
Size: 1323 35 442 35 505
Fragments arranged by size:
1323 505 442 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 978 1080 2160 2340
Size: 978 102 1080 180
Fragments arranged by size:
1080 978 180 102
EaeI y'GGCC_r
Cuts at: 0 181 274 287 314 930 1196 1331 1356
Size: 181 93 13 27 616 266 135 25
Cuts at: 1356 1496 2340
Size: 140 844
Fragments arranged by size:
844 616 266 181 140 135 93 27
25 13
EagI C'GGCC_G
Cuts at: 0 181 274 930 2340
Size: 181 93 656 1410
Fragments arranged by size:
1410 656 181 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 691 766 1054 1235 1591 1723 1756
Size: 307 384 75 288 181 356 132 33
Cuts at: 1756 2047 2116 2206 2233 2340
Size: 291 69 90 27 107
Fragments arranged by size:
384 356 307 291 288 181 132 107
90 75 69 33 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1416 1756 2340
Size: 1416 340 584
Fragments arranged by size:
1416 584 340
EcoO109I rG'GnC_Cy
Cuts at: 0 1052 1731 1740 2106 2181 2240 2340
Size: 1052 679 9 366 75 59 100
Fragments arranged by size:
1052 679 366 100 75 59 9
EcoRI G'AATT_C
Cuts at: 0 961 2340
Size: 961 1379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 990 2340
Size: 990 1350
FspI TGC'GCA
Cuts at: 0 1897 2340
Size: 1897 443
HaeII r_GCGC'y
Cuts at: 0 7 1185 1692 2340
Size: 7 1178 507 648
Fragments arranged by size:
1178 648 507 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 497 529 693 725 808 840 1774 1806
Size: 497 32 164 32 83 32 934 32
Cuts at: 1806 1822 1854 2340
Size: 16 32 486
Fragments arranged by size:
934 497 486 164 83 32 32 32
32 32 16
KasI G'GCGC_C
Cuts at: 0 3 2340
Size: 3 2337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1865 2340
Size: 1865 475
MscI TGG'CCA
Cuts at: 0 289 316 1198 1333 1358 1498 2340
Size: 289 27 882 135 25 140 842
Fragments arranged by size:
882 842 289 140 135 27 25
MslI CAynn'nnrTG
Cuts at: 0 221 835 883 1046 1132 2340
Size: 221 614 48 163 86 1208
Fragments arranged by size:
1208 614 221 163 86 48
MspA1I CmG'CkG
Cuts at: 0 186 338 1192 1655 1795 2340
Size: 186 152 854 463 140 545
Fragments arranged by size:
854 545 463 186 152 140
NarI GG'CG_CC
Cuts at: 0 4 2340
Size: 4 2336
NcoI C'CATG_G
Cuts at: 0 1215 2340
Size: 1215 1125
NspI r_CATG'y
Cuts at: 0 1666 2020 2340
Size: 1666 354 320
Fragments arranged by size:
1666 354 320
PasI CC'CwG_GG
Cuts at: 0 662 1615 2340
Size: 662 953 725
Fragments arranged by size:
953 725 662
PfoI T'CCnGG_A
Cuts at: 0 1700 2340
Size: 1700 640
PpuMI rG'GwC_Cy
Cuts at: 0 1731 1740 2240 2340
Size: 1731 9 500 100
Fragments arranged by size:
1731 500 100 9
PsiI TTA'TAA
Cuts at: 0 452 2340
Size: 452 1888
PspOMI G'GGCC_C
Cuts at: 0 1051 2340
Size: 1051 1289
PspXI vC'TCGA_Gb
Cuts at: 0 2004 2340
Size: 2004 336
PstI C_TGCA'G
Cuts at: 0 35 471 1697 1748 2340
Size: 35 436 1226 51 592
Fragments arranged by size:
1226 592 436 51 35
PvuII CAG'CTG
Cuts at: 0 1192 1795 2340
Size: 1192 603 545
Fragments arranged by size:
1192 603 545
RsrII CG'GwC_CG
Cuts at: 0 279 2340
Size: 279 2061
SanDI GG'GwC_CC
Cuts at: 0 2240 2340
Size: 2240 100
SfcI C'TryA_G
Cuts at: 0 9 31 467 1693 1744 2340
Size: 9 22 436 1226 51 596
Fragments arranged by size:
1226 596 436 51 22 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 313 2340
Size: 313 2027
SfoI GGC'GCC
Cuts at: 0 5 2340
Size: 5 2335
SmlI C'TyrA_G
Cuts at: 0 1222 1305 1513 1810 2004 2340
Size: 1222 83 208 297 194 336
Fragments arranged by size:
1222 336 297 208 194 83
SphI G_CATG'C
Cuts at: 0 2020 2340
Size: 2020 320
StyI C'CwwG_G
Cuts at: 0 89 347 428 600 680 1215 1643 2040
Size: 89 258 81 172 80 535 428 397
Cuts at: 2040 2340
Size: 300
Fragments arranged by size:
535 428 397 300 258 172 89 81
80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 34 744 847 1306 1546 1652 1656 1959
Size: 34 710 103 459 240 106 4 303
Cuts at: 1959 2340
Size: 381
Fragments arranged by size:
710 459 381 303 240 106 103 34
4
TatI w'GTAC_w
Cuts at: 0 122 2340
Size: 122 2218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 658 690 1233 1265 2340
Size: 658 32 543 32 1075
Fragments arranged by size:
1075 658 543 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 483 586 1206 2340
Size: 483 103 620 1134
Fragments arranged by size:
1134 620 483 103
XhoI C'TCGA_G
Cuts at: 0 2004 2340
Size: 2004 336
XmnI GAAnn'nnTTC
Cuts at: 0 2191 2340
Size: 2191 149
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BcgI BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstXI
BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EagI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PfoI PpuMI PsiI PspOMI PspXI PstI PvuII
RsrII SanDI SfcI SfiI SfoI SmlI SphI StyI
TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbvCI BciVI BclI BfrBI BmtI BplI BsaAI BseRI
BsiHKAI BsiWI BsmI BspHI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I ClaI DraI DrdI EarI EciI
EcoICRI EcoRV FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsrI PvuI SacI SacII SalI SapI SbfI ScaI
SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI Tth111I XbaI XmaI ZraI