(Circular) (Six-base) MAPSORT of: pFN21AB5048.seq Check: 5821 from: 1 to: 6877 pFN21AB5048 6877 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2152 2234 3939 2152 Size: 82 1705 5090 Fragments arranged by size: 5090 1705 82 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2673 4687 278 Size: 53 83 186 936 1137 2014 2468 Fragments arranged by size: 2468 2014 1137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2721 4220 1937 Size: 784 1499 4594 Fragments arranged by size: 4594 1499 784 Acc65I G'GTAC_C Cuts at: 4198 4198 Size: 6877 AclI AA'CG_TT Cuts at: 1563 5004 5377 1563 Size: 3441 373 3063 Fragments arranged by size: 3441 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2218 2503 2720 3161 4940 6324 6592 1632 Size: 586 285 217 441 1779 1384 268 1917 Fragments arranged by size: 1917 1779 1384 586 441 285 268 217 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6680 AflIII A'CryG_T Cuts at: 1203 2706 5776 6753 1203 Size: 1503 3070 977 1327 Fragments arranged by size: 3070 1503 1327 977 AgeI A'CCGG_T Cuts at: 5708 5708 Size: 6877 AhdI GACnn_n'nnGTC Cuts at: 5606 5606 Size: 6877 AleI CACnn'nnGTG Cuts at: 1166 2268 1166 Size: 1102 5775 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2700 1184 Size: 1516 5361 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3675 3707 4037 4069 3675 Size: 32 330 32 6483 Fragments arranged by size: 6483 330 32 32 AlwNI CAG_nnn'CTG Cuts at: 2806 3082 3263 3358 4620 6192 2806 Size: 276 181 95 1262 1572 3491 Fragments arranged by size: 3491 1572 1262 276 181 95 ApaI G_GGCC'C Cuts at: 3041 3310 3630 3041 Size: 269 320 6288 Fragments arranged by size: 6288 320 269 ApaLI G'TGCA_C Cuts at: 3596 4934 6090 3596 Size: 1338 1156 4383 Fragments arranged by size: 4383 1338 1156 ApoI r'AATT_y Cuts at: 1493 4186 4412 4564 6673 1493 Size: 2693 226 152 2109 1697 Fragments arranged by size: 2693 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5431 160 Size: 872 4399 1606 Fragments arranged by size: 4399 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6877 AvaI C'yCGr_G Cuts at: 1870 1943 2909 2928 3759 4164 4202 1870 Size: 73 966 19 831 405 38 4545 Fragments arranged by size: 4545 966 831 405 73 38 19 BamHI G'GATC_C Cuts at: 3683 4207 4574 3683 Size: 524 367 5986 Fragments arranged by size: 5986 524 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2840 4198 5653 618 Size: 325 217 627 1053 1358 1455 1842 Fragments arranged by size: 1842 1455 1358 1053 627 325 217 BanII G_rGCy'C Cuts at: 1391 2963 3041 3257 3310 3530 3630 4196 1391 Size: 1572 78 216 53 220 100 566 4072 Fragments arranged by size: 4072 1572 566 220 216 100 78 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2061 2925 3561 928 Size: 971 162 864 636 4244 Fragments arranged by size: 4244 971 864 636 162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2579 2613 3587 3621 4006 4040 5068 Size: 34 642 34 974 34 385 34 1028 Cuts at: 5068 5102 1903 Size: 34 3678 Fragments arranged by size: 3678 1028 974 642 385 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4769 5979 4769 Size: 1210 5667 BclI T'GATC_A Cuts at: 1548 1548 Size: 6877 BfrBI ATG'CAT Cuts at: 2677 2677 Size: 6877 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5488 136 Size: 107 122 71 631 605 3816 1525 Fragments arranged by size: 3816 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4101 6872 4101 Size: 2771 4106 BlpI GC'TnA_GC Cuts at: 4294 4294 Size: 6877 Bme1580I G_kGCm'C Cuts at: 1790 2843 3041 3310 3600 3630 4938 6094 1790 Size: 1053 198 269 290 30 1308 1156 2573 Fragments arranged by size: 2573 1308 1156 1053 290 269 198 30 BmgBI CAC'GTC Cuts at: 1318 2377 1318 Size: 1059 5818 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2108 3532 4608 Size: 416 53 83 399 24 691 1424 1076 Cuts at: 4608 5566 442 Size: 958 1753 Fragments arranged by size: 1753 1424 1076 958 691 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6877 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2443 4084 4153 4190 2443 Size: 1641 69 37 5130 Fragments arranged by size: 5130 1641 69 37 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2874 3514 3989 4064 5537 1239 Size: 851 784 640 475 75 1473 2579 Fragments arranged by size: 2579 1473 851 784 640 475 75 Bpu10I CC'TnA_GC Cuts at: 3256 3347 3359 3256 Size: 91 12 6774 Fragments arranged by size: 6774 91 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5002 5867 6165 5002 Size: 865 298 5714 Fragments arranged by size: 5714 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3440 5540 882 Size: 617 1941 2100 2219 Fragments arranged by size: 2219 2100 1941 617 BsaAI yAC'GTr Cuts at: 493 1204 2709 4668 493 Size: 711 1505 1959 2702 Fragments arranged by size: 2702 1959 1505 711 BsaBI GATnn'nnATC Cuts at: 6871 6871 Size: 6877 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2670 4684 5066 5701 Size: 53 83 186 936 1137 2014 382 635 Cuts at: 5701 275 Size: 1451 Fragments arranged by size: 2014 1451 1137 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5310 5708 5982 6129 6540 5310 Size: 398 274 147 411 5647 Fragments arranged by size: 5647 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2945 2975 3512 3542 4037 Size: 30 165 30 1542 30 537 30 495 Cuts at: 4037 4067 1178 Size: 30 3988 Fragments arranged by size: 3988 1542 537 495 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3422 4019 4112 1186 Size: 2236 597 93 3951 Fragments arranged by size: 3951 2236 597 93 BseYI C'CCAG_C Cuts at: 2741 2799 3470 6080 6576 2741 Size: 58 671 2610 496 3042 Fragments arranged by size: 3042 2610 671 496 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3917 1163 Size: 573 2181 4123 Fragments arranged by size: 4123 2181 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4327 5088 6116 1540 Size: 275 186 2326 761 1028 2301 Fragments arranged by size: 2326 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 3600 4938 5023 6094 2076 Size: 1524 1338 85 1071 2859 Fragments arranged by size: 2859 1524 1338 1071 85 BsmI GAATG_Cn' Cuts at: 3151 4388 4481 3151 Size: 1237 93 5547 Fragments arranged by size: 5547 1237 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2843 2963 3041 3257 3310 3530 Size: 399 286 767 120 78 216 53 220 Cuts at: 3530 3600 3630 4196 4938 5023 6094 1391 Size: 70 30 566 742 85 1071 2174 Fragments arranged by size: 2174 1071 767 742 566 399 286 220 216 120 85 78 70 53 30 BspEI T'CCGG_A Cuts at: 6540 6540 Size: 6877 BspHI T'CATG_A Cuts at: 3237 4766 6496 3237 Size: 1529 1730 3618 Fragments arranged by size: 3618 1730 1529 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2152 2234 2913 3939 4232 844 Size: 1308 82 679 1026 293 3489 Fragments arranged by size: 3489 1308 1026 679 293 82 BsrBI CCG'CTC Cuts at: 1394 1823 3963 4764 1394 Size: 429 2140 801 3507 Fragments arranged by size: 3507 2140 801 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2752 3132 3168 5372 5546 65 Size: 1183 1504 380 36 2204 174 1396 Fragments arranged by size: 2204 1504 1396 1183 380 174 36 BsrFI r'CCGG_y Cuts at: 1700 1810 3806 5521 5708 1700 Size: 110 1996 1715 187 2869 Fragments arranged by size: 2869 1996 1715 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6877 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6877 BssSI C'ACGA_G Cuts at: 4937 5949 4937 Size: 1012 5865 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6877 BstBI TT'CG_AA Cuts at: 5681 5681 Size: 6877 BstEII G'GTnAC_C Cuts at: 1184 2386 1184 Size: 1202 5675 BstXI CCAn_nnnn'nTGG Cuts at: 2612 2971 3830 4042 2612 Size: 359 859 212 5447 Fragments arranged by size: 5447 859 359 212 BstYI r'GATC_y Cuts at: 1972 2637 3078 3213 3683 4101 4207 4574 4959 Size: 665 441 135 470 418 106 367 385 Cuts at: 4959 4976 6428 6514 6526 6872 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 665 470 441 418 385 367 346 135 106 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2336 2336 Size: 6877 BtgI C'CryG_G Cuts at: 513 1065 2004 2964 513 Size: 552 939 960 4426 Fragments arranged by size: 4426 960 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2224 2509 3925 487 Size: 683 56 998 285 1416 3439 Fragments arranged by size: 3439 1416 998 683 285 56 BtsI GCAGTG_nn' Cuts at: 3877 4393 5187 5207 3877 Size: 516 794 20 5547 Fragments arranged by size: 5547 794 516 20 ClaI AT'CG_AT Cuts at: 1554 3393 1554 Size: 1839 5038 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6842 DraI TTT'AAA Cuts at: 4181 4181 Size: 6877 DraIII CAC_nnn'GTG Cuts at: 3751 4062 4114 3751 Size: 311 52 6514 Fragments arranged by size: 6514 311 52 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5884 809 Size: 507 4568 1802 Fragments arranged by size: 4568 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2329 2733 2895 Size: 54 1425 50 275 18 499 404 162 Cuts at: 2895 3030 3733 3808 3897 4324 5213 6842 8 Size: 135 703 75 89 427 889 1629 43 Fragments arranged by size: 1629 1425 889 703 499 427 404 275 162 135 89 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4324 1537 Size: 275 2512 4090 Fragments arranged by size: 4090 2512 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3183 4807 1353 Size: 1830 1624 3423 Fragments arranged by size: 3423 1830 1624 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3944 5464 5838 5984 387 Size: 1064 138 2355 1520 374 146 1280 Fragments arranged by size: 2355 1520 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2218 2503 2720 2874 3161 3514 Size: 393 458 128 285 217 154 287 353 Cuts at: 3514 3989 4064 4940 5537 6324 6592 1239 Size: 475 75 876 597 787 268 1524 Fragments arranged by size: 1524 876 787 597 475 458 393 353 287 285 268 217 154 128 75 EcoNI CCTnn'n_nnAGG Cuts at: 2417 3350 2417 Size: 933 5944 EcoO109I rG'GnC_Cy Cuts at: 2501 2902 3037 3038 3306 3307 3355 4132 4142 Size: 401 135 1 268 1 48 777 10 Cuts at: 4142 2501 Size: 5236 Fragments arranged by size: 5236 777 401 268 135 48 10 1 1 EcoRI G'AATT_C Cuts at: 4186 4564 4186 Size: 378 6499 FseI GG_CCGG'CC Cuts at: 3810 3810 Size: 6877 FspI TGC'GCA Cuts at: 5383 5383 Size: 6877 HaeII r_GCGC'y Cuts at: 1678 2884 6024 1678 Size: 1206 3140 2531 Fragments arranged by size: 3140 2531 1206 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2264 2296 2622 2654 3157 Size: 32 135 32 692 32 326 32 503 Cuts at: 3157 3189 3512 3544 3644 3676 5518 5550 5592 Size: 32 323 32 100 32 1842 32 42 Cuts at: 5592 5624 1373 Size: 32 2626 Fragments arranged by size: 2626 1842 692 503 326 323 135 100 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2125 2344 2650 3091 4221 4467 Size: 988 281 187 219 306 441 1130 246 Cuts at: 4467 669 Size: 3079 Fragments arranged by size: 3079 1130 988 441 306 281 246 219 187 HindIII A'AGCT_T Cuts at: 748 748 Size: 6877 HpaI GTT'AAC Cuts at: 4467 4467 Size: 6877 KpnI G_GTAC'C Cuts at: 4202 4202 Size: 6877 MfeI C'AATT_G Cuts at: 4476 4476 Size: 6877 MluI A'CGCG_T Cuts at: 6753 6753 Size: 6877 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5991 6175 1305 Size: 4686 184 2007 Fragments arranged by size: 4686 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2735 2897 10 Size: 54 1425 343 903 162 3990 Fragments arranged by size: 3990 1425 903 343 162 54 MslI CAynn'nnrTG Cuts at: 518 1166 2268 2610 2682 2858 3828 4835 5194 Size: 648 1102 342 72 176 970 1007 359 Cuts at: 5194 5353 518 Size: 159 2042 Fragments arranged by size: 2042 1102 1007 970 648 359 342 176 159 72 MspA1I CmG'CkG Cuts at: 1606 2399 4293 4970 6118 6363 1606 Size: 793 1894 677 1148 245 2120 Fragments arranged by size: 2120 1894 1148 793 677 245 NaeI GCC'GGC Cuts at: 3808 3808 Size: 6877 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5386 Fragments arranged by size: 5386 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6877 NgoMIV G'CCGG_C Cuts at: 3806 3806 Size: 6877 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6877 NotI GC'GGCC_GC Cuts at: 4324 4324 Size: 6877 NsiI A_TGCA'T Cuts at: 2679 2679 Size: 6877 NspI r_CATG'y Cuts at: 1132 1229 3389 4235 5780 1132 Size: 97 2160 846 1545 2229 Fragments arranged by size: 2229 2160 1545 846 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6877 PciI A'CATG_T Cuts at: 5776 5776 Size: 6877 PflMI CCAn_nnn'nTGG Cuts at: 1108 2806 3522 1108 Size: 1698 716 4463 Fragments arranged by size: 4463 1698 716 PmeI GTTT'AAAC Cuts at: 4181 4181 Size: 6877 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4037 4069 4946 4978 6459 6491 4037 Size: 32 877 32 1481 32 4423 Fragments arranged by size: 4423 1481 877 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2902 4132 2902 Size: 1230 5647 PshAI GACnn'nnGTC Cuts at: 1613 3839 1613 Size: 2226 4651 PsiI TTA'TAA Cuts at: 4447 4447 Size: 6877 PspOMI G'GGCC_C Cuts at: 3037 3306 3626 3037 Size: 269 320 6288 Fragments arranged by size: 6288 320 269 PstI C_TGCA'G Cuts at: 830 3046 3921 4229 830 Size: 2216 875 308 3478 Fragments arranged by size: 3478 2216 875 308 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6877 SalI G'TCGA_C Cuts at: 1936 4219 1936 Size: 2283 4594 SbfI CC_TGCA'GG Cuts at: 4229 4229 Size: 6877 ScaI AGT'ACT Cuts at: 5125 6837 5125 Size: 1712 5165 SfcI C'TryA_G Cuts at: 826 1045 3042 3917 4225 5360 6041 6232 826 Size: 219 1997 875 308 1135 681 191 1471 Fragments arranged by size: 1997 1471 1135 875 681 308 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6877 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6877 SmaI CCC'GGG Cuts at: 1872 3761 4204 1872 Size: 1889 443 4545 Fragments arranged by size: 4545 1889 443 SmlI C'TyrA_G Cuts at: 820 1017 1943 4981 5882 6144 820 Size: 197 926 3038 901 262 1553 Fragments arranged by size: 3038 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4668 493 Size: 4175 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6877 SphI G_CATG'C Cuts at: 1132 4235 1132 Size: 3103 3774 SspI AAT'ATT Cuts at: 5 52 4801 5 Size: 47 4749 2081 Fragments arranged by size: 4749 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6877 StyI C'CwwG_G Cuts at: 513 1065 2004 2898 3330 3516 3622 3633 4316 Size: 552 939 894 432 186 106 11 683 Cuts at: 4316 513 Size: 3074 Fragments arranged by size: 3074 939 894 683 552 432 186 106 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 2978 3076 3464 4904 4921 5074 Size: 1255 609 789 98 388 1440 17 153 Cuts at: 5074 5259 5704 325 Size: 185 445 1498 Fragments arranged by size: 1498 1440 1255 789 609 445 388 185 153 98 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3192 3813 5123 Size: 274 80 33 51 1443 1215 621 1310 Cuts at: 5123 6835 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1310 1215 621 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2945 2977 3414 3446 6837 6869 2945 Size: 32 437 32 3391 32 2953 Fragments arranged by size: 3391 2953 437 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3265 1316 Size: 1949 4928 XbaI T'CTAG_A Cuts at: 4213 4213 Size: 6877 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6877 XmaI C'CCGG_G Cuts at: 1870 3759 4202 1870 Size: 1889 443 4545 Fragments arranged by size: 4545 1889 443 XmnI GAAnn'nnTTC Cuts at: 2922 5006 2922 Size: 2084 4793 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2671 4685 276 Size: 53 83 186 936 1137 2014 2468 Fragments arranged by size: 2468 2014 1137 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI