Restriction map for
pFN21AB5048
(Linear) (Six-base) MAPSORT of: KIBB5048.seq Check: 5834 from: 1 to: 2172
KIBB5048.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 147 229 1934 2172
Size: 147 82 1705 238
Fragments arranged by size:
1705 238 147 82
AatII G_ACGT'C
Cuts at: 0 668 2172
Size: 668 1504
AccI GT'mk_AC
Cuts at: 0 716 2172
Size: 716 1456
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 498 715 1156 2172
Size: 213 285 217 441 1016
Fragments arranged by size:
1016 441 285 217 213
AflIII A'CryG_T
Cuts at: 0 701 2172
Size: 701 1471
AleI CACnn'nnGTG
Cuts at: 0 263 2172
Size: 263 1909
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 695 2172
Size: 695 1477
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1670 1702 2032 2064 2172
Size: 1670 32 330 32 108
Fragments arranged by size:
1670 330 108 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 801 1077 1258 1353 2172
Size: 801 276 181 95 819
Fragments arranged by size:
819 801 276 181 95
ApaI G_GGCC'C
Cuts at: 0 1036 1305 1625 2172
Size: 1036 269 320 547
Fragments arranged by size:
1036 547 320 269
ApaLI G'TGCA_C
Cuts at: 0 1591 2172
Size: 1591 581
AvaI C'yCGr_G
Cuts at: 0 904 923 1754 2159 2172
Size: 904 19 831 405 13
Fragments arranged by size:
904 831 405 19 13
BamHI G'GATC_C
Cuts at: 0 1678 2172
Size: 1678 494
BanI G'GyrC_C
Cuts at: 0 835 2172
Size: 835 1337
BanII G_rGCy'C
Cuts at: 0 958 1036 1252 1305 1525 1625 2172
Size: 958 78 216 53 220 100 547
Fragments arranged by size:
958 547 220 216 100 78 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 920 1556 2172
Size: 56 864 636 616
Fragments arranged by size:
864 636 616 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 574 608 1582 1616 2001 2035 2172
Size: 574 34 974 34 385 34 137
Fragments arranged by size:
974 574 385 137 34 34 34
BfrBI ATG'CAT
Cuts at: 0 672 2172
Size: 672 1500
BglII A'GATC_T
Cuts at: 0 2096 2172
Size: 2096 76
Bme1580I G_kGCm'C
Cuts at: 0 838 1036 1305 1595 1625 2172
Size: 838 198 269 290 30 547
Fragments arranged by size:
838 547 290 269 198 30
BmgBI CAC'GTC
Cuts at: 0 372 2172
Size: 372 1800
BmrI ACTGGGnnnn_n'
Cuts at: 0 103 1527 2172
Size: 103 1424 645
Fragments arranged by size:
1424 645 103
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 438 2079 2148 2172
Size: 438 1641 69 24
Fragments arranged by size:
1641 438 69 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 869 1509 1984 2059 2172
Size: 85 784 640 475 75 113
Fragments arranged by size:
784 640 475 113 85 75
Bpu10I CC'TnA_GC
Cuts at: 0 1251 1342 1354 2172
Size: 1251 91 12 818
Fragments arranged by size:
1251 818 91 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 1435 2172
Size: 1435 737
BsaAI yAC'GTr
Cuts at: 0 704 2172
Size: 704 1468
BsaHI Gr'CG_yC
Cuts at: 0 665 2172
Size: 665 1507
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 940 970 1507 1537 2032 2062 2172
Size: 940 30 537 30 495 30 110
Fragments arranged by size:
940 537 495 110 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1417 2014 2107 2172
Size: 1417 597 93 65
Fragments arranged by size:
1417 597 93 65
BseYI C'CCAG_C
Cuts at: 0 736 794 1465 2172
Size: 736 58 671 707
Fragments arranged by size:
736 707 671 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1912 2172
Size: 1912 260
BsiHKAI G_wGCw'C
Cuts at: 0 71 1595 2172
Size: 71 1524 577
Fragments arranged by size:
1524 577 71
BsmI GAATG_Cn'
Cuts at: 0 1146 2172
Size: 1146 1026
Bsp1286I G_dGCh'C
Cuts at: 0 71 838 958 1036 1252 1305 1525 1595
Size: 71 767 120 78 216 53 220 70
Cuts at: 1595 1625 2172
Size: 30 547
Fragments arranged by size:
767 547 220 216 120 78 71 70
53 30
BspHI T'CATG_A
Cuts at: 0 1232 2172
Size: 1232 940
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 147 229 908 1934 2172
Size: 147 82 679 1026 238
Fragments arranged by size:
1026 679 238 147 82
BsrBI CCG'CTC
Cuts at: 0 1958 2172
Size: 1958 214
BsrDI GCAATG_nn'
Cuts at: 0 747 1127 1163 2172
Size: 747 380 36 1009
Fragments arranged by size:
1009 747 380 36
BsrFI r'CCGG_y
Cuts at: 0 1801 2172
Size: 1801 371
BstEII G'GTnAC_C
Cuts at: 0 381 2172
Size: 381 1791
BstXI CCAn_nnnn'nTGG
Cuts at: 0 607 966 1825 2037 2172
Size: 607 359 859 212 135
Fragments arranged by size:
859 607 359 212 135
BstYI r'GATC_y
Cuts at: 0 632 1073 1208 1678 2096 2172
Size: 632 441 135 470 418 76
Fragments arranged by size:
632 470 441 418 135 76
Bsu36I CC'TnA_GG
Cuts at: 0 331 2172
Size: 331 1841
BtgI C'CryG_G
Cuts at: 0 959 2172
Size: 959 1213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 219 504 1920 2172
Size: 219 285 1416 252
Fragments arranged by size:
1416 285 252 219
BtsI GCAGTG_nn'
Cuts at: 0 1872 2172
Size: 1872 300
ClaI AT'CG_AT
Cuts at: 0 1388 2172
Size: 1388 784
DraIII CAC_nnn'GTG
Cuts at: 0 1746 2057 2109 2172
Size: 1746 311 52 63
Fragments arranged by size:
1746 311 63 52
EaeI y'GGCC_r
Cuts at: 0 324 728 890 1025 1728 1803 1892 2172
Size: 324 404 162 135 703 75 89 280
Fragments arranged by size:
703 404 324 280 162 135 89 75
EarI CTCTTCn'nnn_
Cuts at: 0 1178 2172
Size: 1178 994
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1939 2172
Size: 1939 233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 213 498 715 869 1156 1509 1984
Size: 85 128 285 217 154 287 353 475
Cuts at: 1984 2059 2172
Size: 75 113
Fragments arranged by size:
475 353 287 285 217 154 128 113
85 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 412 1345 2172
Size: 412 933 827
Fragments arranged by size:
933 827 412
EcoO109I rG'GnC_Cy
Cuts at: 0 496 897 1032 1033 1301 1302 1350 2127
Size: 496 401 135 1 268 1 48 777
Cuts at: 2127 2137 2172
Size: 10 35
Fragments arranged by size:
777 496 401 268 135 48 35 10
1 1
FseI GG_CCGG'CC
Cuts at: 0 1805 2172
Size: 1805 367
HaeII r_GCGC'y
Cuts at: 0 879 2172
Size: 879 1293
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 617 649 1152 1184 1507 1539
Size: 259 32 326 32 503 32 323 32
Cuts at: 1539 1639 1671 2172
Size: 100 32 501
Fragments arranged by size:
503 501 326 323 259 100 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 120 339 645 1086 2172
Size: 120 219 306 441 1086
Fragments arranged by size:
1086 441 306 219 120
MscI TGG'CCA
Cuts at: 0 730 892 2172
Size: 730 162 1280
Fragments arranged by size:
1280 730 162
MslI CAynn'nnrTG
Cuts at: 0 263 605 677 853 1823 2172
Size: 263 342 72 176 970 349
Fragments arranged by size:
970 349 342 263 176 72
MspA1I CmG'CkG
Cuts at: 0 394 2172
Size: 394 1778
NaeI GCC'GGC
Cuts at: 0 1803 2172
Size: 1803 369
NgoMIV G'CCGG_C
Cuts at: 0 1801 2172
Size: 1801 371
NsiI A_TGCA'T
Cuts at: 0 674 2172
Size: 674 1498
NspI r_CATG'y
Cuts at: 0 1384 2172
Size: 1384 788
PflMI CCAn_nnn'nTGG
Cuts at: 0 801 1517 2172
Size: 801 716 655
Fragments arranged by size:
801 716 655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2032 2064 2172
Size: 2032 32 108
Fragments arranged by size:
2032 108 32
PpuMI rG'GwC_Cy
Cuts at: 0 897 2127 2172
Size: 897 1230 45
Fragments arranged by size:
1230 897 45
PshAI GACnn'nnGTC
Cuts at: 0 1834 2172
Size: 1834 338
PspOMI G'GGCC_C
Cuts at: 0 1032 1301 1621 2172
Size: 1032 269 320 551
Fragments arranged by size:
1032 551 320 269
PstI C_TGCA'G
Cuts at: 0 1041 1916 2172
Size: 1041 875 256
Fragments arranged by size:
1041 875 256
SfcI C'TryA_G
Cuts at: 0 1037 1912 2172
Size: 1037 875 260
Fragments arranged by size:
1037 875 260
SmaI CCC'GGG
Cuts at: 0 1756 2172
Size: 1756 416
StyI C'CwwG_G
Cuts at: 0 893 1325 1511 1617 1628 2172
Size: 893 432 186 106 11 544
Fragments arranged by size:
893 544 432 186 106 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 973 1071 1459 2172
Size: 184 789 98 388 713
Fragments arranged by size:
789 713 388 184 98
TatI w'GTAC_w
Cuts at: 0 1187 1808 2172
Size: 1187 621 364
Fragments arranged by size:
1187 621 364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 940 972 1409 1441 2172
Size: 940 32 437 32 731
Fragments arranged by size:
940 731 437 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1260 2172
Size: 1260 912
XmaI C'CCGG_G
Cuts at: 0 1754 2172
Size: 1754 418
XmnI GAAnn'nnTTC
Cuts at: 0 917 2172
Size: 917 1255
ZraI GAC'GTC
Cuts at: 0 666 2172
Size: 666 1506
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbsI
BcgI BfrBI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I FseI
HaeII Hin4I HincII MscI MslI MspA1I NaeI NgoMIV
NsiI NspI PflMI PpiI PpuMI PshAI PspOMI PstI
SfcI SmaI StyI TaqII TatI TstI Tth111I XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BclI
BglI BlpI BmtI BpuEI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV FalI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NcoI NdeI NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI
XcmI XhoI