Restriction map for
pFN21AB5029
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI AspEI
AviII BamHI BbeI BbvCI BcgI
BclI BfrI BglII BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrFI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HpaI KasI
KspI MamI MfeI MluI MroI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1361 GGTACC G^GTACC
AccI 1555 GTCTAC GT^[AC][GT]AC
AccII 257 CGCG CG^CG
AflIII 338 ACATGT A^C[GA][CT]GT
AocI 38 CCTCAGG CC^T[ACGT]AGG
ApaI 590 GGGCCC GGGCC^C
Asp718I 1361 GGTACC G^GTACC
AspHI 1126 GAGCAC G[AT]GC[AT]^C
AspI 694 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1395 CCTAGG C^CTAGG
BbrPI 1366 CACGTG CAC^GTG
BbsI 267 GAAGAC GAAGAC(2/6)
BbvI 1475 GCTGC GCAGC(8/12)
BciVI 249 GGATAC GTATCC(6/5)
BcnI 1298 CCCGG CC^[GC]GG
BlnI 1395 CCTAGG C^CTAGG
BpuAI 267 GAAGAC GAAGAC(2/6)
BsaAI 1366 CACGTG [CT]AC^GT[GA]
BsiHKAI 1126 GAGCAC G[AT]GC[AT]^C
BsmFI 50 GTCCC GGGAC(10/14)
BspLU11I 338 ACATGT A^CATGT
BsrBI 777 CCGCTC CCGCTC(-3/-3)
BsrDI 1260 CATTGC GCAATG(2/0)
BssHII 256 GCGCGC G^CGCGC
BssSI 630 CACGAG CACGAG(-5/-1)
BstAPI 357 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 257 CGCG CG^CG
BstXI 807 CCAGAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 38 CCTCAGG CC^T[ACGT]AGG
Eco81I 38 CCTCAGG CC^T[ACGT]AGG
Fnu4HI 1475 GCTGC GC^[ACGT]GC
HapII 1299 CCGG C^CGG
HincII 1219 GTTGAC GT[CT]^[GA]AC
HindII 1219 GTTGAC GT[CT]^[GA]AC
HpaII 1299 CCGG C^CGG
ItaI 1475 GCTGC GC^[ACGT]GC
KpnI 1361 GGTACC GGTAC^C
MspI 1299 CCGG C^CGG
MvnI 257 CGCG CG^CG
NciI 1298 CCCGG CC^[GC]GG
NcoI 938 CCATGG C^CATGG
NspI 338 ACATGT [GA]CATG^[CT]
PflFI 694 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 240 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1366 CACGTG CAC^GTG
PmlI 1366 CACGTG CAC^GTG
PshAI 1222 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 241 CAGCTG CAG^CTG
ScaI 173 AGTACT AGT^ACT
TseI 1475 GCTGC G^C[AT]GC
Tsp45I 679 GTCAC ^GT[GC]AC
Tth111I 694 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 240 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 420 CCAGAAACAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 1135 GGCGTC G[GA]^CG[CT]C
1226 GGCGTC
Asp700I 394 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
411 GAAAAAGTTC
AvaI 64 CCCGAG C^[CT]CG[GA]G
470 CCCGAG
BalI 755 TGGCCA TGG^CCA
935 TGGCCA
BanII 590 GGGCCC G[GA]GC[CT]^C
889 GAGCCC
BglI 748 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
937 GCCATGGAGGC
BpmI 1145 CTGGAG CTGGAG(16/14)
1501 CTGGAG
BsaHI 1135 GGCGTC G[GA]^CG[CT]C
1226 GGCGTC
BsmI 1455 GAATGC GAATGC(1/-1)
398 GCATTC
BsoBI 64 CCCGAG C^[CT]CG[GA]G
470 CCCGAG
BspHI 11 TCATGA T^CATGA
1139 TCATGA
BstYI 359 AGATCC [GA]^GATC[CT]
1327 GGATCT
DrdI 691 GACGACCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1108 GACCTTATTGTC
DsaI 938 CCATGG C^C[GA][CT]GG
1265 CCACGG
EaeI 755 TGGCCA [CT]^GGCC[GA]
935 TGGCCA
EcoT14I 938 CCATGG C^C[AT][AT]GG
1395 CCTAGG
FauI 776 CCCGC CCCGC(4/6)
926 CCCGC
Hin1I 1135 GGCGTC G[GA]^CG[CT]C
1226 GGCGTC
HindIII 1090 AAGCTT A^AGCTT
1342 AAGCTT
MaeII 1367 ACGT A^CGT
1388 ACGT
MaeIII 679 GTCAC ^GT[ACGT]AC
1076 GTTAC
MflI 359 AGATCC [GA]^GATC[CT]
1327 GGATCT
MluNI 755 TGGCCA TGG^CCA
935 TGGCCA
MscI 755 TGGCCA TGG^CCA
935 TGGCCA
MspA1I 241 CAGCTG C[AC]G^C[GT]G
280 CAGCGG
PpuMI 48 GGGTCCC [GA]G^G[AT]CC[CT]
1529 AGGACCC
PstI 355 CTGCAG CTGCA^G
406 CTGCAG
RcaI 11 TCATGA T^CATGA
1139 TCATGA
StyI 938 CCATGG C^C[AT][AT]GG
1395 CCTAGG
TaqI 288 TCGA T^CGA
402 TCGA
TfiI 285 GAATC G^A[AT]TC
1198 GATTC
TthHB8I 288 TCGA T^CGA
402 TCGA
XhoII 359 AGATCC [GA]^GATC[CT]
1327 GGATCT
XmnI 394 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
411 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BfaI BmyI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII FokI
HaeIII HgaI HhaI HinP1I HinfI
HphI Ksp632I MaeI MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
Tsp509I TspRI